tersebut, urutan alel dari keturunan atau anak, dapat diidentifikasi dan dianalisa sejauh mana tingkat kekerabatan dan keturunan anak tersebut. Berdasarkan alel yang muncul, dapat juga dianalisa, tingkat ketepatan diagnosa atau uji kekerabatan dari sampel yang diuji, dalam hal ini berapa prosentase ketepatan dari uji tersebut untuk dapat diyakini, bahwa hasil yang diperoleh mendekati 100%.
Selanjutnya untuk menguji apakah mikrosatelit tersebut dapat digunakan sebagai marker dalam uji keturunan dan identitas ternak dilakukan penghitungan EXP (Exclusion Probability) dan kombinasi EXP (cEXP) ) (Botstein et al., 1980). Sehingga, pengujian tersebut lebih meyakinkan apakah sampel tersebut benar-benar sesuai dengan pola yang diturunkan dari kedua tetuanya. Adapun rumus penentuan EXP adalah sebagai berikut :
dimana :
: Jumlah Alel
P
u ,P
v : Tingkat kemunculan relative alel ke u dan ke v pada lokusSelain itu, melalui analisa mikrosatelit tersebut, dimana dari hasil PCR dapat dilakukan penghitungan heterosigositas dari suatu populasi, yang didapatkan dari frekuensi dan jumlah alel yang dihasilkan berdasarkan rumus yang dikemukakan oleh Nei (1987) sebagai berikut :
dimana :
He : Heterozygositas k : Jumlah Alel
Pi : Frekuensi Alel dari Alel ke i N : Jumlah Sampel Ternak
KESIMPULAN
Dari uraian tersebut, maka dapat ditarik kesimpulan sebagai berikut :
1. Analisa Mikrosatelit dapat digunakan untuk pengujian keturunan dan tingkat kekerabatan pada ternak
2. Analisa Mikrosatelit merupakan metode analisa yang mempunyai tingkat ketepatan tinggi dengan analisa yang simpel, mudah dan menggunakan sedikit sampel
3. Analisa Mikrosatelit secara aplikasi dapat diterapkan dalam rangka seleksi ternak yang lebih ekonomis karena hasil yang diperoleh tidak memerlukan waktu lama
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