THE AND STEROID CONTENT OF SOME DEEP SEA
SPECIES FROM WEST OCEAN
Department of Product and
dun I Fisheries. Research Ministry Marine Affairs Fisheries showed about 529
kinds of deep sea fish. Some of those are presumed to be containing
as pauciradiatus, splendens,
Haplosthethus Hoplothethus sp, The work is
I the color of liquid changes to
cup of of was cold put to the filter cup, than it was diluted with 200 water, contain nitrogen, added sotne boiled
and 100 in order rha t solution became base. filter cup was
% Protein Nitrogen conversion factor
Where A = titration = acid
= titration (ml) =
I The cold fat cup was in desiccators constant
phase. The completing a. Acid hydrolysis
After that, by vacuum pressure 50
dun Perikonon I
l'he condition of occurred is:
1 . Color :
Steroid extraction was done based a reported by Touchtone and Kasparov (1 970) referred by Riris of deep sea at 20 grams.
dan I - 17-18 Juli 2007
111. AND DISCUSSION
1 . Test
of pmxitnate analysis of deep sea shows that there are of each sea fish. It depends on at species, age the at area 1969 by Septarina ! 999).
Table The of analysis
: A h
A 3 : ,
A 4 : crassipinus 10 :
A 5 I :
shows that o f deep sea fish's protein varies from
to 24.8 Based Olcott cited by 1998, deep fish's
is in high level at protein and low it comparing with pelagic fish, the of sea fish's is higher at pelagic fish,
shows in Table 2 that crassipinus contain the highest at
protein of 27.4% with other researched sea fish.
- 17-18 2007
of fat was shown in figure The of Fat Deep Sea
I Fat content
iA 4 A5 A6 A7 A9 A11
Figure 2. of percentage of sea fish's fat level Water level
sea has high salinity with 31.2 The high
rate of inorganic salt To the condition of is
deep solution body for adapting. The
condition causes water of fish is less of fresh pelagic
I . . of . . C o n t e n t of . Deep
Konferensi Sains I - Drornago, 7.18 Juli 2007
2. Amino Test
Table 3. of deep Sea Fish
: Benthodesmus :
: Ophidiidae : Ostracoberyu
: Myctophidae sp
Marine fish have 17 amino acids which are required by Nine of those are acid the other essential
acids. Both Dietinoides pauciradiatus and the
greatest quantity of atni acid and and
splanden have the amino acids Mean
sp, Myctophidae the
highest of amino acid Ophidiidac
Ostracoberyu have highest in
From all of deep sea fish analyzed, that is dominant in the all kinds of ainino acid those is an essential
acid and include in hearth. Other
acids is and 1994). The function
of acid a s biochemical by to
produced energy, then to stimulate up part of body for reflection
having as aprodisiach was in great quantity deep sea moreover it has potential as of strong medicine
with (NO) having role in width of
of ophidiidae were dominated by
acid. gives flavors and delicious smelt, the as
cod, but it was sweater acid is a amino acid, change to glucose
and glycogen by called glucogenic 1994).
Other ainino acid asparagin, acid,
glutamin, Prolin dominated in pauciradiatus and
They are glocogenic ainino acid.
The kinds of acids which are of ketogenic and
stcrol such as which will give positive (Dence,
THP 3 3
- I 17- Juli
The result the shows deep sea fishes has
content. It was seen in Liebennan Test, extract of isolation using gave result, changing extract's color to green. and ergosterol.
The of Fish great effect was shown pauciradiatus,
The changing extract color
to shows the of sterol-stigma organic indicate the existence of two more double of carbon. While extract by using ethyl acetate did not show the changing of color.
Steroid is white crystal. Steroid has shapes such little needle,
leaf, plate depends on kind of solution in crystallization, skill
and luck of Since steroid has or carbon atoms,
this component to undeleted in water & Ethyl
acetate water has possibility the steroid which is not restricted, caused the polarity of ethyl acetate is higher than chlorofonn,
The color of which does not change is predicted by the
saturated steroid It is caused the saturated chonestanol
shows negative toward test 1980).
positive result toward
test. All of the are expected showing nearness the of steroid. fish proximate, protein
w a s higher pelagic The highest content is in Hoplothethus sp, at 27.4%. In acid deep sea fish had 17 acids, 9
ofwhich essential acid and the others were non essential acid.
in the quantity some deep sea fish. Meanwhile steroid test
- Dramagn 2007
Burchard, 8 kinds of sea fish content steroid and the
is in and crassipinus
The Secret of Deep Sea. ber
Amino Acids. ZOOS].
A, D, NL, Sedamawati, S. 1 989. The
Hint of Food Analysis Laboratory. tics,
of Fishery and Research. Study Fish in Indonesian. Jakarta: of Marine and
Institution Fishery and 2005. Study Deep Sea
Stock in Suth Java and West Jakarta: Department of Marine
B.J. dan J.A. 1988. Nutrient, and Agricultural.
Translate of General Dircstorate of High
G, to Biology. Company.
A.L. Basic of Biochemical. 2. Publisher Jakarta
M.A., dan Hith: of
Education Culture. General of High Education. of
J Marine Biology. A Ecological Jakarta Publisher
(Ed). 992. Seafood Handbook, Advanced, The Benefit;
'Taste, ion Rackland. Maine.
G.M. and B. W. Tucker. 1 Sea ; Effects of technology on Marcel Inc. New York and
FPIK - 7-1 8
J . Effect of Protein and Oil in toward Quantity and
Composition of Amino Acid Brain Acid and the ability of rat
[thesis]. of Post hgriculturat University.
1999. Evaluation point pH, Power of and
of Fresh in different quality. [scripts]. Faculty
Fisheries Marine Agricultural University
J, Research of Deep Sea Fish in
Suseno, dan A. 2005. The of and Amino Acid
of Deep Sra Fish Ocean. Proceeding of
Seminar of Medicines.
1992. Chemical of Food and Jakarta:
Konferensi Sains Kelautan dan Perikanan Indonesia I Kampus FPIK - IPB Dramaga. 1 7-18 Juli 2007
THE NUTRIENT AND STEROID CONTENT OF SOME DEEP SEA FISH
SPECIES FROM WEST SUMATERA OCEAN
Sugeng Heri Suseno1), Ali Suman2),Wudianto2) and Arin Damayanti1)
Department of Fisheries Product Technology, Faculty of Fisheries and Marine Science;2)Institution of Marine and Fishery, Department of Marine and Fishery
The Purpose o f this research is to observe 1he content of nutrient and steroid of some deep sea fish from west Sumarera ocean. The result o f the research shows that I I kinds of deep sea fish such as: Dietmoides
pauciradiniin. Bcnihodeimuy tenuis, Beryx splendent, Haplasthethus iraaaipint/Xt Hoplothethtis sp, Ophidiidae, Osimobviyu dotyge, Godamus collt'tfits. Hyteragfype japontca contain protein 23,0-24,8 %, fat 1,9-4,1 %. carbohydrate 0-1,75 %, ash 1,7-2,4 %, water 70.1-72,1 %. In amino acid test, it can be identified 17 amino acid (9 essential amino acid and 8 non essential amino acid) Meanwhile in steroid test using Libermann Burchad, it can be identified 8 kinds of deep sea fish containing steroid and the greatest conccntratton is in Dietmoides pauciradiatus, Benthodesmus cenuis, Beryx splendens, Haplusthethus craaaipinus.
Based on the total prediction of Indonesian fishery ocean potential which is amount to 6.6 million tons/year, consist of 5 million ton in Indonesian ocean and 2.1 million tons in Zee ocean. The potential prediction comes from some kinds of ocean fish, such as the small pelagic fish 3.5 tons, coral fish 0.048 million tons/year (Anonymous 2000).
According to the potential estimation, production and ulilizaUon of pelagic fish in Indonesia in 2001, Malaka Street and Java Sea are in the state of over fishing (BRKP 2001) therefore it needs to look for new fishing ground area instead of area in coastal area and pelagic area. Part of ocean environment which is predicted as alternative area is deep sea area,
Deep sea area is located under shining dept area in the open ocean and deeper than continental shelf (>200m). The habitat is the widest pan in the world where seldom organisms live in; its water volume is predicted of amount up to 85% of 70% world surface (Nybakken 1992).