ICoBio 2015
International Conference on Bosciences
Program Book
Table of Contents
Welcome Speech of Chairman of Organizing Committee ... 1
Opening Remark of Dean of the Faculty of Mathematics and Natural Sciences ... 3
Opening Remark of Rector of Bogar Agricultural University ... 4
Committee ... 6
Conference Program ... 7
IPB ICC Conference Layout.. ... lO Speaker Profiles ... ll Abstracts of Keynote and Invited Speakers ... 25
Oral and Poster Presentation Schedule ... 37
Abstracts of Oral Presentation ... 63
Abstracts of Poster Presentation ... 193
Tour destination: Taman Mini Indonesia Indah and Bogar Botanical Garden ... 237
Bogar Interesting Places ... 238
Common Indonesian Language ... 239
Index ... 240
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International Conference on Biosciences (ICoBio) 2015 · Bogor, 5-7 August 2015 /CoBio.eventipb.ac.id
Welcome Speech of Chair
Our knowledge in understanding of application or explo industrial, medical, advances in applie implications, such a sources, the develo1 of understanding disorders. Nevertheless, the preseno molecular biology, bioinformatics, bic between biological and social bioscienc better world. Advances in biology wil individuals and appreciate human biodi
International Conference on Bioscie "Appreciating Unity in Diversity". Thil current trends in research and teachin approaches that are important for unt Moreover, to encourage the formation stakeholders to accelerate our efforts I their applications.
The ICoBio 2015 is attended by particiJ Malaysia, India, Pakistan, Germany, Th< register are 220 participants. Howevt accepted as a presenter, so there is s' (attendant). Due to the wide scope of bit four main groups focusing on Biodi· physiological, developmental, and beha biotechnology, and omic technology (!
biology (group 4).
In addition, a series of activities were <
namely the activity of "One day lecture i August-Universitat Gi.ittingen, German) Bogar entitled "Symposium on Bogor Institute of Science and Technology (J<
Through those activity the participan regarding bioinformatic and the current
left blank.
, 5-7 August ZOlS
Welcome Speech of Chairman of Organizing Committee
Our knowledge in biology has allowed us to achieve a better understanding of many biological phenomena and their application or exploitation in agriculture, food safety and security, industrial, medical, and environmental conservation. However, advances in applied biology ca'l also produce some negative implications, such as reducing biodiversity, the imbalance in food sources, the development of new agents of infection, and the lack of understanding of non-infectious diseases or metabolic disorders. Nevertheless, the presence of the interdisciplinary sciences such as molecular biology, bioinformatics, bio-nanotechnology, and even the relationship between biological and social biosciences, will be a very useful to help us in creating a better world. Advances in biology will be able to expand our views to be unique individuals and appreciate human biodiversity to live in peace and harmony.
International Conference on Biosciences (ICoBio) 2015 taking the theme of "Appreciating Unity in Diversity". This conference is intended to gain insight into current trends in research and teaching related to biology, such as interdisciplinary approaches that are important for understanding the biology and its applications. Moreover, to encourage the formation of networks between biologists and relevant stakeholders to accelerate our efforts to understand the biological phenomena and their applications.
The ICoBio 2015 is attended by participants from several countries including japan, Malaysia, India, Pakistan, Germany, Thailand, and Indonesia. Total participants who register are 220 participants. However not all participant who register can be accepted as a presenter, so there is some participant suggested as non-presenter (attendant). Due to the wide scope of bioscience, the ICoBio 2015 topic is divided into four main groups focusing on Biodiversity, ecology, and evolution (group 1); physiological, developmental, and behavioral sciences (group 2); Molecular biology, biotechnology, and omic technology (group 3); and Applied and interdisciplinary biology (group 4).
In addition, a series of activities were also conducted before the conference begins, namely the activity of "One day lecture in Bioinformatics by Prof Rolf Daniel - Georg-August-Universitat Gi.ittingen, Germany" and mini symposium NAIST japan - IPB Bogor entitled "Symposium on Bogor Agricultural University (Indonesia) - Nara Institute of Science and Technology (Japan) Graduate Student Exchange Program". Through those activity the participants are expected to gain extensive insight regarding bioinformatic and the current techniques in modern biotechnology.
The I CoBia 2015 is the first international conference organized by the Department of Biology, Faculty of Mathematics and Natural Sciences, Bogar Agricultural University, Indonesia and is expected to serve as an initial step to be held continuously every two years (bianually). This activity is also the first step in the framework of collaboration between the Faculty of Mathematics and Natural Sciences (especially Department of Biology) Bogar Agricultural University, Indonesia with the Faculty of Science, Kasetsart University, Thailand. Through such activities will be able to foster a love of bioscience and the exchange of information on bioscience and the development of networking between researchers. I am sure you will be able to find on the conference program is really fun, encourage intellectual and also provide important insights on the way forward how to appreciate bioscience.
Special welcome is intended to young scientists and students on whom the future of biosciences depends. You have a great opportunity at this meeting to get to know the main leaders in field of bioscience and to network with your colleagues. The need for international collaboration is essential to take advantage of bioscience and related sciences optimally.
Finally, I hope you will find your experience in Bogar, Indonesia to be unique, satisfying, and enjoyable.
With warmest welcome,
Prof. Aris Tri Wahyudi
Chairman
Organizing Committee
International Conference on Biosciences (ICoBio) 2015- Bogar,· 5-7 Augu st 201 5 ICoBio.event. ipb.ac.id
Opening Remark of Dean o:
Natur
Bismillahirrahmanirrahim
In the name of Allah the Most Gracious ·
Ladies and gentlemen
First of all I would like to express my keynote and invited speakers for carr. Conference on Biosciences (!CoBia ~ Faculty of Mathematics and Natural : forum is very important to all of us, res countries, to share and discuss new vie Your contribution in this seminar will and research in biosciences, it will alsc our life and our future. Biosciences con the Conference "Appreciating Unity in utilize our diverse knowledge to explo1 and sustainability of humanity.
On this occasion, I especially would li~
Kasetsart University, Thailand for cooperation with the Faculty of Math en University, particularly in the area cooperation will mutually benefit and s
Last but not least, I also would like to I of Biology, FMIPA-IPB, all of whom international seminar. I hope this sem advantages to all participants.
Dr. Jr. Sri Nurdiati, M.Sc
conference organized by the Department of Sciences, Bogor Agricultural University,
step to be held continuously every two first step in the framework of collaboration Natural Sciences (especially Department of Indonesia with the Faculty of Science,
activities will be able to foster a love of on bioscience and the development of you will be able to find on the conference and also provide important insights on
and students on whom the future of at this meeting to get to know the network with your colleagues. The need for take advantage of bioscience and related
Bogor, Indonesia to be unique,
ICoBio.eventipb.ac.id
3
Opening Remark of Dean of the Faculty of Mathematics and
Natural Sciences
Bismil/ahirrahmanirrahim
In the name of Allah the Most Gracious the Most Merciful
Ladies and gentlemen
First of all I would like to express my sincere gratitude to all participants especially keynote and invited speakers for coming to this international event, International Conference on Biosciences (ICoBio 2015) organized by Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. This forum is very important to all of us, researchers from Indonesia as well as from other countries, to share and discuss new views and information in the field of biosciences. Your contribution in this seminar will not only support and strengthen our teaching and research in biosciences, it will also demonstrate how important bioscience is for our life and our future. Biosciences comprise many disciplines, therefore the theme of the Conference "Appreciating Unity in Diversity" should be able to encourage us to utilize our diverse knowledge to explore nature in a spirit of harmony for the benefit and sustainability of humanity.
On this occasion, I especially would like to thank the Dean of the Faculty of Science, Kasetsart University, Thailand for her presence which served to strengthen cooperation with the Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, particularly in the area of Biological Sciences. We expect that this cooperation will mutually benefit and strengthen researches in both universities.
Last but not least, I also would like to thank to the entire team from the Department of Biology, FMIPA-IPB, all of whom worked hard to prepare and organize this international seminar. I hope this seminar will be successful and will provide many advantages to all participants.
Dr. Ir. Sri Nurdiati, M.Sc
Opening Remark of Rector of Bogor Agricultural University
Ladies and Gentlemen,
Welcome to Bogar, Indonesia
Welcome to Bogar Agricultural University, Indonesia
It is an honor for us upon your arrival this morning to attend the "International Conference on Biosciences (!CoBia) 2015" held in Bogar Agricultural University (Institut Pertanian Bogar, IPB), Indonesia. This conference is an important part in relation to the basics and applied sciences related to bioscience. In this case, the basic science is an important part in the development of the applied sciences, especially in IPB, is also stated in the vision of IPB which will be the front-runner in strengthening the nation's dignity through excellent higher education on a global level in agriculture, marine and tropical biosciences. In addition, lPB is also a university that is heading toward an international research based university, so that this conference will be an important moment to achieve the IPB vision.
Through this opportunity, we would like to express our gratitude to Director General of Learning and Student Affairs, Ministry of Research, Technology and Higher Education, Prof. In tan Ahmad; our collaborators speakers (Prof Hiroshi Takagi - Nara Institute of Science and Technology, japan; Prof Rolf Daniel - Georg-August-Universitat Giittingen, Germany; Prof Supa Hannongbua - Kasetsart University, Thailand; and Dr. Mashuri Waite- University of Hawaii, USA) and other speakers (Dr. Reymond Tjandrawinata - PT Dexa Medica jakarta, Indonesia; Prof Daniel Murdiyarso - Bogar Agricultural University, Indonesia; Dr. Achmad Dinoto - Indonesian Institute of Sciences, Indonesia; Dr. Miftahudin - Bogar Agricultural University, Indonesia; and Dr. Seiji Mori- Ibaraki University, japan) for their participation in ICoBio 2015. Also, I would like to give sincere gratitude to the Organizing Committee and Steering Committee for the efforts they provide for this international conference .
The main theme of this conference is the "International Conference on Biosciences: Unity in Diversity". This theme is in accordance with the present condition, where we can meet each other and exchange information on the development of biosciences in Indonesia in particular, and also in the world. Through this conference, we also expect an establishment of network or cooperation between researchers or participants. ICoBio 2015 is organized by Department of Biology, Faculty of Mathematics and Natural Sciences, Bogar Agricultural University, as a forum of knowledge sharing in very wide and global research area in field of bioscience and others related sciences. This suggests that IPB is active in the development of science and technology, not only in Indonesia but also in the world.
International Conference on Biosciences (ICoBio) 2015- Bogar, 5-7 Augu st 2015 ICoBio.eventipb.ac.id
Finally I express my sincere gratitude t< of !CoBia 2015.
Thank you for your participation in th1 be enjoyable.
Rector of Bogar Agricultural University
Prof Herry Suhardiyanto
Bogor Agricultural University
Indonesia
morning to attend the "International " held in Bogar Agricultural University This conference is an important part in to bioscience. In this case, the basic of the applied sciences, especially in will be the front-runner in strengthening higher education on a global level in In addition, IPB is also a university that based university, so that this conference IPB vision.
express our gratitude to Director General of Research, Technology and Higher speakers (Prof Hiroshi Takagi - Nara Prof Rolf Daniel - Georg-August-Hannongbua - Kasetsart University, of Hawaii, USA) and other speakers (Dr. Jakarta, Indonesia; Prof Daniel Murdiyarso Dr. Achmad Dinoto - Indonesian Institute Agricultural University, Indonesia; and r their participation in !CoBia 2015. Also, I the Organizing Committee and Steering
international conference.
"International Conference on Biosciences: with the present condition, where we on the development of biosciences in . Through this conference, we also expect n between researchers or participants. of Biology, Faculty of Mathematics and , as a forum of knowledge sharing in of bioscience and others related sciences. of science and technology, not
Bogor, 5-7 August 2015
Finally I express my sincere gratitude to all participants who attended the conference of !CoBia 2015.
Thank you for your participation in the !CoBia 2015 and I hope this conference will be enjoyable.
Rector of Bogar Agricultural University - Indonesia
Prof Herry Suhardiyanto
Committee
Steering Committe
ProfDr Antonius Suwanto (Chairman) ProfDr Hiroshi Takagi
Prof Dr Alex Hartana Prof Dr uharsono Dr Dedi Duryadi Solihin Prof Dr Aris Tri Wahyudi
Organizing Committee
ProfDr Aris Tri Wahyudi (Chairman) Prof Dr YM Diah
Ratnadewi (Secretary) Dr Rika Raffiudin (Secretary) Dr Triadiati
Dr Berry }uliandi Mafrikhul Muttaqin, MSi Dr Miftahudin
Dr Tatik Chikmawati Dr Dyah Perwitasari Prof Dr Anja Meryandini
Dr Utut Widyastuti Dr Sulistijorini Dr Sri Listyowati Dr Nisa Rachmania Dr Kanthi Arum Puji Rianti, MSi Dr Achmad Farajallah Dr Tri Atmowidi DrYohana CS Dr Aris Tjahjoleksono
The Committee of International Conference on Biosciences (ICoBio) 2015 Department of Biology, Faculty of Mathematics and Natural Sciences Darmaga Campuss, Bogor Agricultural University (IPB)
}1. Agathis-Kamp':ls IPB Darmaga Bogor 16680 Indonesia Website: http:/ jicobio.event.ipb.ac.id/
International Conference on Biosciences (ICoBio) 2015- Bogar, 5-7 August 2015
ICoBio.eventipb.ac.id
Confere1
WEDNESDAY, AUGUST 5, 2015 Time allocation 08.00-08.45 08.45-09.00 09.00-09.10 Activity Registration Welcome speech: Chair of Organizing Co Opening Speech: Rector of IPB
Plenary Session
Keynote Speaker: Prof. Dr. In tan Ahmad:
09.10-09.30 General of Learning ar Affairs, Ministry of Rel Technology and Highe 09.30-10.00 Prof. Dr. Supa Hannollj
10.00-10.15 Morning tea break
Invited Speakers:
10.15-10.45 Prof. Dr. Rolf Daniel 10.45-11.15 Dr. Achmad Dinoto 11.15-11.45 Prof. Dr. Daniel Murdi)
11.45-12.00 Discussion
12.00-12.30 Poster Introduction Pr (P01-P22)
12.30-13.30 Lunch
13.30-14.00 Poster Session
14.00-16.00 Parallel Oral Session
16.00-16.15 Afternoon tea break
16.15-17.15 Parallel Oral Session
18.45-21.00 Welcome Dinner
Dr Utut Widyastuti Dr Sulistijorini Dr Sri Listyowati Dr Nisa Rachmania Dr Kanthi Arum Puji Rianti, MSi Dr Achmad Farajallah Dr Tri Atmowidi Dr Yohana CS Dr Aris Tjahjoleksono
on Biosciences (!CoSio) 2015 and Natural Sciences rsity (IPB)
Indonesia
Boga r. 5· 7 August
Conference Program
I
WEDNESDAY, AUGUST 5, 2015 Time allocation 08.00-08.45 08.45-09.00 09.00-09.10 Activity Registration Welcome speech:Chair of Organizing Committee Opening Speech:
Rector of IPB
Plenary Session 09.10-09.30 09.30-10.00 10.00-10.15 10.15-10.45 10.45-11.15 11.15-11.45 11.45-12.00 12.00-12.30 12.30-13.30 13.30-14.00 14.00-16.00 16.00-16.15 16.15-17.15 18.45-21.00 Keynote Speaker:
Prof. Dr. lntan Ahmad: Director General of Learning and Student Affairs, Ministry of Research, Technology and Higher Education
Prof. Dr. Supa Hannongbua
Morning tea break
Invited Speakers:
Prof. Dr. Rolf Daniel Dr. Achmad Dinoto
Prof. Dr. Daniel Murdiyarso
Discussion
Poster Introduction Presentation (P01-P22)
Lunch
Poster Session
Parallel Oral Session
Afternoon tea break
Parallel Oral Session
Welcome Dinner
Room
IPB International
Ballroom 1 & 2
Ballroom 1 & 2
Ballroom 1 & 2
Ballroom 1 & 2
IPB ICC
Ballroom 1 & 2
Ballroom 1 & 2
IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D Ballroom 1 & 2
THURSDAY, AUGUST 6, 2015
Time
allocation Activity Room
Plenary Session 09.00-09.30 09.30-09.45 09.45-10.15 10.15-10.45 10.45-11.15 11.15-11.30 11.30-12.00 12.00-12.30 12.30-13.30 13.30-15.30 15.30-15.45 15.45-16.45 16.45-17.00 Keynote Speaker:
Dr. Raymond Tjandrawinata
Morning tea break
Invited Speakers:
Prof. Dr. Hiroshi Takagi
Dr. Mashuri Waite
Dr. Miftahudin
Discussion
Poster Introduction Presentation (P23-P42)
Poster Session
Lunch
Parallel Oral Session
Afternoon tea break
Parallel Oral Session
Closing Ceremony
and see you in !CoBio 2017
Ballroom 1 & 2
IPB ICC
Ballroom 1 & 2
Ballroom 1 & 2
IPB ICC
IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D Dean of the Faculty of Mathematics and Natural Sciences, IPB
Friday, August 7, 2015: Excursion
07.00
08.30-14.00
14.00
Depart to Beautiful Indonesia Miniature Park (Taman Mini Indonesia Indah, TMJJ), jakarta
TMII: visit the Pavilliun of East java, West Sumatra, Papua, Sulawesi, Komodo Dragon Park, Lunch, *Friday pray for moslems at
Diponegoro Prince Mosque
Depart to Bogor or to Soekarno Hatta International Airport
International Conference on Biosciences (ICoBio) 2015 · Bogor, 5-7 August 201 5 /CoBio.eventipb.ac. id
Side event
1. One day Lecture on Bioinformatics
The lecture is given by Prof. Dr. Rolf Da1 held at Meeting Room of Faculty of Ma1 4, 2015 from 9 am-4 pm. This even\ collaboration with CRC 990 -EFForTS.
Topics of lecture: (1) Next-generation analyze and assemble data derivl Genomeftransciptome analysis and f
metatranscricptomic approaches, (4) microbial communities
2. Symposium on Bogor Agriculture Science and Technology (Japan) Grac
The symposium is held at the Researc Bogor Agricultural University, Gedung August 2015, from 9 am-3 pm. This ev collaboration with Research Center Agricultural University. During the sy Agricultural University and Nara lnst research results. The topic of symposiu on plant, microbe and animal.
8
Room
Ballroom 1 & 2
IPB ICC
Ballroom 1 & 2
~ entation
Ballroom 1 & 2
IPB ICC
IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D IPB ICC
Ballroom 1, Ballroom 2, Meeting Room A,B,C,D Dean of the Faculty of
17 Mathematics and Natural
Sciences, IPB
.. . .
1nes1a Miniature Park (Taman Mini IJakarta
iof East java, West Sumatra, Papua, Sulawesi, fnch, *Friday pray for moslems at
~ue
ekarno Hatta International Airport
i · Bogor, 5·7 August 2015 /CoBio.eventipb.ac.id
i
Side event
1. One day Lecture on Bioinformatics
The lecture is given by Prof. Dr. Rolf Daniel (Georg-August-Universitiit Gottingen) and held at Meeting Room of Faculty of Mathematics and Natural Sciences IPB on August 4, 2015 from 9 am-4 pm. This event is organized by Department of Biology in collaboration with CRC 990-EFForTS.
Topics of lecture: (1) Next-generation sequencing technologies and ways to initiale analyze and assemble data derived from the different technologies, (2) Genomeftransciptome analysis and functional annotation, (3) Metagenomic and metatranscricptomic approaches, ( 4) Taxonomic and functional assessment of microbial communities
2. Symposium on Bogor Agricultural University (Indonesia}-Nara Institute of Science and Technology (Japan} Graduate Student Exchange Program
The symposium is held at the Research Center for Bioresources and Biotechnology, Bogor Agricultural University, Gedung PAU, Jl. Kamper, Kampus IPB Darmaga on 4 August 2015, from 9 am-3 pm. This event is organized by Department of Biology in collaboration with Research Center for Bioresources and Biotechnology, Bogor Agricultural University. During the symposium, the graduate students from Bogor Agricultural University and Nara Institute of Science and Technology share their research results. The topic of symposium covers molecular biology and biotechnology on plant, microbe and animal.
DEVELOPMENTAL AND BEHAVIORAL
Lestari
Kamon
TITLE
Productivity and Specific Growth Rate of Mixed Microalagae Cultured in Outdoor Pond
Physiological Responses of Transgenic Tobacco (Nicotiana
tabacum L.) Carrying an Aluminum
Tolerance Gene Candidate to Aluminum Stress
The Role of Phosphorus in
Improving Sorghum Crop (Sorghum bicolor L. Moench) Tolerance to Aluminum Stress
Salinity Stress Affects the Decrease of Cab Gene Expression and
""n~r<'""'"etic Rate in Maize
The Metabolic Regulation
Mechanism and Physiological Role ofValine in Yeast
Inhibition of Soft Coral
Nephthyigorgia sp. Extract Against
Melanin Biosynthesis in
The Cytotoxic Effect of
Methoxyacetic Acid on Mll Cell Line of the Swiss Webster Mice Emb
Disturbance of Cortical Microtubule Orientation by Sulfamethizole Leads to Abnormal Secondary Cell Wall Patterning during Xylem Vessel Cell Differentiation
15.18-15.30 Discussion
15.30-15.45 AFTERNOON TEA BREAK
MODERATOR: Prof. Dr. Diah Ratnadewi
15.45-15.57 PDB-0-21
15.57-16.09 PDB-0-22
16.09-16.21 PDB-0-23
16.21-16.33 PDB-0-24
16.33-16.45 Pornsawan Sutthinon Nilubol Nuanjunkong Ari Sunandar FAUZIYAH HARAHAP Discussion
Microspore, Pollen Development anrl. Callose Wall Deposition of Microspore Tetrad in Mangosteen
(Garcinia mangostana L.) Compared
with Seachore Mangosteen
(Garcinia hombroniana Pierre.)
Features of Embryo Development and Oil Reserves of Jatropha curcas
in Relation to Pollination Methods Induction of Somatic
Embryogenesis in Sengon
(Fa/cataria molluccana) through
Thidiazuron, Proline, and Light Treatments
Callus Induction and Viability of Mangosteen (Garcinia Mangostana
L.) from in vitro Bud
168
EMIBillYOtGE:NE:SIS IN SENGON (Falcataria
PROLINE, AND LIGHT TREATMENTS
em a tics and Natural Sciences, Bogar Darmaga, Bogar 16680 Indonesia address: encedarmo@ipb.ac.id
growing leguminous tree and important for ia. In order to meet the Indonesia future F. culture of F. molluccana is an alternative the large-scale of plant propagation. The the effect of thidiazuron, proline, and light in F. molluccana. The effect of thidiazuron (continuous light, seven days dark then to of somatic embryogenesis in leaf explant of somatic embryo was obtained in 5 !lM days followed by incubation at 25±2 oC •wu"""'"nted by 1.2 gjl proline. The effect of of somatic embryogenesis in leaf explant increasing of proline concentrations could Histological analysis was confirmed the , asymmetric division in epidermis were found in cotyledon-stage and indicated embryogenesis. This study suggested the somatic embryogenesis in F. molluccana for
molluccana, somatic embryogenesis,
- Bogar, 5-7 August 2015
169
PDB0 24: CALLUS INDUCTION AND VIABILITY OF MANGOSTEEN (Garcinia
mangostana L.) FROM IN VITRO BUD
Fauziyah Harahap l', Suci Rahayu 2, Hasruddin l, Cicik Surianil, Devi Riana Sagala l
Department of Biology, Faculty of Mathematics and Natural Sciences, State Universi ty of Medan, UNIMED,jln. Willem Iskandar Psr V Medan Estate, 20221 , Indonesia .. Tel :
+6281376817918, Fax : +626161 '3319.
Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatera,]ln.Bioteknologi No.1 , Kampus USU Medan, 20155, Indonesia.
' Corresponding author e-mail address: fauz iyahharahap@gmail.com
Mangosteen is a unique tropical fruit that originated from Indonesia that has a good prospect. One of the main problem is the slow rate of growth of this plant, so it is difficult to get seed. The need to produce numerous and good quality plantlet in a short time has been carried out with in vitro culture. To solve the problem is getting from callus induction by tissue culture method. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro mangosteen callus induction. In vitro bud were treated with six level treatment, a) without plant growth regulator, b) 1 ppm 2,4 D, c) 1 ppm 2,4 D and 1 ppm Kinetin, d) 1 ppm 2,4 D and 0.5 ppm TDZ, e) 1 ppm Kinetin and 0.5 ppm TDZ, f) 1 ppm 2,4 D and 1 ppm Kinetin and 0.5 ppm TDZ for callus induction in Murashige and Skoog (MS) medium. After five week treatment, callus is transfered to 0.5 ppm NAA + 0.2 ppm Kinetin + five concentrations of Benzyl Amino Purine (BAP) (0, 1, 2, 3, 4 ppm) . Our result show that plant growth regulator positively affected mangosteen callus induction (time of callus appearance, biomassa, colour, texture, browning). The best medium for mangosteen callus induction was found to be MS + 1 ppm Kinetin + 0.5 ppm Thidiazuron. After transfer, colour, size and texture of the callus is changes, but not for biomassa.
Key words : Mangosteen, callus, 2,4 D, TDZ, Kinetin.
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INTERNASIONAL
:
o'CallusInduction And
Viability
Of
Mangosteen
(Garcinta
mangostanaL.)
From
In
VitroBuil'
:
Fauziyah Harahap, Suci Rahayu, Hasruddin,Cicik
Suriani,Devi
Riana Sagala:
a. JudulProsiding
: International conference on Bosciencesb.ISBN
c. Tahun
Terbit
d. Penerbit
e. Jumlah halaman
918-602-711-681-8 August 2015
Agricultural University
(IPB )169 PDBO 24
Kategori Publikasi
Makalah
W
(beri
u'pada kategori yang tepat)l-l
l{asil
Penilaian Peer Review :Pros iding Folum
Ilmiah
Internasional Prctsiding Forum Ilmiah NasionalKomponen
Yang
Dinilai
Nilai
Maksimal ProsidinNilaiAkhir
Yang Diperoleh
a. Kelengkapan unsur isi artikel (10%)
b. Ruang lingkup dan kedalaman pembahasan (309/o)
c. Kecukupan dan kemutahiran
data/infornasi
dand. Kelengkapan unsur dan kualitas penerbit (30%)
Totat
=
(100%)
Medan,
September 2015Reviewer
-
3a
Prof.
Dr.
Herbert
Sipahutar,
MS,
M.Sc
NrP.
19610626 198710
1001