ABSTRAK
HUBUNGAN EKSPRESI IMUNOHISTOKIMIA
VASCULAR ENDOTHELIAL GROWTH FACTOR
(
VEGF
) DAN
TUMOR-INFILTRATING LYMPHOCYTES
(
TILs
) DENGAN TIPE
HISTOPATOLOGI DAN STADIUM KLINIS KARSINOMA NASOFARING
Nancy Sartika Tambunan
1, Soekimin
1, Joko S. Lukito
1 1Departemen Patologi Anatomik Fakultas Kedokteran USU
Latar belakang. Karsinoma nasofaring merupakan karsinoma yang berasal dari mukosa nasofaring yang secara mikroskopis tampak berupa diferensiasi skuamosa. Beberapa target molekuler telah diidentifikasi dalam spesimen tumor penderita karsinoma nasofaring. VEGF
merupakan faktor proangiogenik, yang berperan dalam angiogenesis untuk pertumbuhan tumor, invasi, dan metastasis tumor. Limfosit T dikenal sebagai main effector dari respon imun anti tumor, yang sering dihubungkan dengan gambaran klinis yang lebih baik. Penderita karsinoma nasofaring dapat diklasifikasikan berdasarkan stadium klinis dengan menggunakan sistem TNM.
Tujuan. Untuk mengetahui hubungan ekspresi imunohistokimia VEGF dan Tumor-infiltrating lymphocytes (TILs) dengan tipe histopatologi dan stadium klinis karsinoma nasofaring.
Bahan dan Metode. Penelitian ini merupakan penelitian deskriptif analitik dengan pendekatan cross sectional. Sampel pada penelitian ini adalah slaid dan blok parafin dari jaringan nasofaring yang telah didagnosa sebagai karsinoma nasofaring. Blok parafin tersebut kemudian dipulas dengan Hematoxilin-Eosin dan imunohistokimia VEGF. Ekepresinya akan dianalisa dengan Kruskal-Wallis untuk melihat hubungannya dengan tipe histopatologi dan stadium klinis karsinoma nasofaring.
Hasil. Berdasarkan analisa statistik Kruskal-Wallis terhadap 42 sampel kasus karsinoma nasofaring menguji hubungan ekspresi imunohistokimia VEGF dengan tipe histopatologi karsinoma nasofaring, diperoleh p-value = 0,501 (p>0,05), hubungan ekspresi imunohistokimia VEGF dengan stadium klinis karsinoma nasofaring, diperoleh p-value = 0,772 (p>0,05), hubungan TILs dengan tipe histopatologi karsinoma nasofaring, diperoleh p-value = 0,884 (p>0,05) dan hubungan TILs dengan stadium klinis karsinoma nasofaring, diperoleh p-value = 0,886 (p>0,05).
Simpulan. Tidak ada hubungan yang bermakna antara ekspresi imunohistokimia VEGF dan TILs dengan tipe histopatologi dan stadium klinis karsinoma nasofaring.
Kata kunci : karsinoma nasofaring, VEGF, TILs, tipe histopatologi, stadium klinis
ABSTRACT
CORRELATION BETWEEN THE EXPRESSION OF
IMMUNOHISTOCHEMISTRY VASCULAR ENDOTHELIAL GROWTH
FACTOR (VEGF) AND TUMOR-INFILTRATING LYMPHOCYTES (TILs)
WITH HISTOPATHOLOGICAL TYPE AND CLINICAL STAGING OF
NASOPHARYNGEAL CARCINOMA
Nancy Sartika Tambunan
1, Soekimin
1, Joko S. Lukito
1 1Department of Anatomical Pathology, Faculty of Medicine, USU
Background. Nasopharyngeal carcinoma is a carcinoma arising in the nasopharyngeal mucosa that shows light microscopic of squamous differentiation. Several molecular targets have been identified in tumour specimen of patients with nasopharyngeal carcinoma. VEGF is an angiogenesis factor, which contributes to angiogenesis and subsequent growth, invasion and metastasis of tumours. T lymphocytes are recognized as the main effectors of anti tumor immune responses. They often associated with better clinical outcomes. Nasopharyngeal carcinoma patients can be classified according to their clinical stage in the system. The system that we used are TNM stage, T-stage and metastasis of nasopharyngeal carcinoma.
Objective. To investigate correlation between the expression of immunohistochemistry Vascular Endothelial Growth Factor (VEGF) and Tumor-infiltrating lymphocytes (TILs) with histopathological type and clinical staging of nasopharyngeal carcinoma.
Material and Methods. This is an analytical descriptive study with cross sectional approach. The sample of this study are slides and paraffin blocks of nasopharyngeal tissue which diagnose histopatologically as nasopharyngeal carcinoma. Then the samples were stained with Hematoxilin Eosin and VEGF. Their expressions will be analyzed with Kruskal-Wallis statistical analysis to investigate the correlation with histopathological type and clinical staging of nasopharyngeal carcinoma.
Results. Based on an analysis statistic Kruskal-Wallis test to 42 sample cases nasopharyngeal carcinoma to evaluated the correlation between the expression of VEGF with histopathological type with p-value = 0,501 (p>0,005), the correlation between the expression of VEGF with clinical staging with p-value = 0,772 (p>0,005), the correlation between TILs with histopathological type with p-value = 0,884 (p>0,005), the correlation between TILs with clinical staging with p-value = 0,886 (p>0,005).
Conclution. There is no significant correlation between the expression of immunohistochemistry VEGF and TILs with histopathological type and clinical staging of nasopharyngeal carcinoma.
Keywords: nasopharyngeal carcinoma, VEGF, TILs, histopathological type, clinical staging
