LAMPIRAN 2 Distribusi Ukuran Droplet Emulsi Tipe wo Tabel Ukuran Droplet Air Sediaan Emulsi Tipe wo

(1)

(2)

(3)

LAMPIRAN 2

Distribusi Ukuran Droplet Emulsi Tipe w/o

Tabel Ukuran Droplet Air Sediaan Emulsi Tipe w/o

Perbesaran skala okuler = 10 x

Perbesaran skala obyektif = 10 x

1 skala obyektif = 0.01 mm

Skala okuler ∑ Droplet Ukuran (µm)

(Skala okuler x 9,71)

(4)

Rentang = nilai max – nilai min

Interval kelas = Rentang kelas

Jumlah kelas

= 5,83/9

= 0,65

Jarak ukuran (µm) Rata-rata jarak ukuran ∑ droplet dalam tiap ukuran

0,97-1,62 1,30 89

1,63-2,28 1,45 148

2,29-2,94 2,61 43

2,95-3,60 3,27

-3,61-4,26 3,93 9

4,27-4,92 4,59 7

4,93-5,58 5,25

-5,59-6,24 5,91 1

6,25-,6,90 6,57 3

(5)

m nd

nd

d_wm 4,05



3691,66 4693,30

3 4

 

 



(6)

LAMPIRAN 3

Distribusi Ukuran Droplet Mikroemulsi Etanol 1:6 Tipe w/o

Diameter Droplet

(nm)

Jumlah (%)

139,8 38,26

154,4 25,19

170,4 15,73

188,1 9,35

207,7 5,42

229,3 2,98

253,1 1,59

279,1 0,85

308,4 0,43

340,5 0,21

100,00

Rentang = nilai max – nilai min

= 253,1-139,8

= 200,7

Jumlah kelas = 1 + 3,3 log n

= 1+ 3,3 log 100

= 1 + 6,6

= 7,6~ 8

Interval kelas = Rentang kelas

Jumlah kelas

= 200,7/8

(7)

Jarak ukuran (µm) Rata-rata jarak ukuran ∑ droplet dalam tiap ukuran(%)

139,8-164,89 152,35

63,45

164,90-189,99 177,45

25,08

190,00-215,09 202,55

5,42

215,1-240,19 227,65

2,98

240,20-265,29 252,75

1,59

265,30-290,39 277,85

0,85

290,4-315,49 302,95

0,43

315,5-340,59 328,05

0,21

∑ = 100,00

(8)

LAMPIRAN 4

Hasil Uji Homogenitas Natriuml Diklofenak dalam Sediaan Gel

Perlakuan sampel :

250,0 mg sediaan (mengandung 1% natrium diklofenak )

≈

100 ppm + PBS

ad 25,0 mL, ambil 0,5 ml + PBS sampai 10,0 mL (ukur serapan)

≈

5 ppm

Contoh perhitungan :

data diambil dari Formula I replikasi 1

Penimbangan = 250,8 mg

Tabel Pengukuran Homogenitas Natriuml Diklofenak

dalam Basis Gel Carbomer 940

Formula

Penimbangan

(mg)

Absorban

Kadar

(ppm)

1 _0,2508 _0,167 _5,3429

2 _0,2504 _0,17 _5,438

I

3 _0,2503 _0,174 _5,5659

1 _250,5 _0,161 _5,1375

2 _250,0 _0,161 _5,1375

II

3 _250,0 _0,168 _5,3829

Tabel Hasil Perhitungan Homogenitas Natriuml Diklofenak

dalam Basis Gel Carbomer-940

Formula

% Konsentrasi natrium diklofenak

Rata-rata ± SD

108,76 ± 2,338 2,15

1 102,54

2 102,75

II

3 107,32

(9)

LAMPIRAN 5

Hasil Uji Daya Sebar Sediaan Gel Natrium Diklofenak

Kurva hubungan antara diameter penyebaran (cm) vs beban (g)

Diameter Penyebaran (cm)

Formula 1 Formula 2

Beban

(g)

R1 R2 R3 Rata-rata ± SD R1 R2 R3 Rata-rata ± SD

0 _{4,90 5,50 4,50} _4,97_±_{0,50 10,20}_11,40_{12,30 11,30}_±_1,05

5 _{5,20 5,70 4,70} _5,20_±_{0,50 10,70}_12,70_{13,70 11,83}_±_1,53

10 _{5,30 5,90 4,70} _5,30_±_{0,60 10,80}_13,30_{14,00 12,53}_±_1,68

15 _{5,50 5,90 4,90} _5,43_±_{0,50 11,00}_13,60_{14,30 12,83}_±_1,74

20 _{5,50 6,10 4,90} _5,50_±_{0,60 11,30}_13,80_{14,40 3,07}_±_1,64

25 _{5,60 6,10 4,90} _5,53_±_{0,60 11,40}_13,90_{14,40 13,20}_±_1,61

30 _{5,60 6,10 5,00} _5,57_±_{0,55 11,40}_13,90_{14,40 13,23}_±_1,61

35 _{5,60 6,10 5,00} _5,57_±_{0,55 11,40}_13,90_{14,40 13,23}_±_1,61

40 _{5,70 6,10 5,00} _5,60_±_{0,56 11,40}_13,90_{14,40 13,23}_±_1,61

45 _{5,90 6,20 5,00} _5,70_±_{0,62 11,40}_13,90_{14,40 13,23}_±_1,61

50 _{5,90 6,20 5,00} _5,70_±_{0,62 11,40}_13,90_{14,40 13,23}_±_1,61

(10)

LAMPIRAN 6

Hasil Uji Pelepasan Natrium Diklofenak

1. Tabel perhitungan Formula I (Replikasi 1)

Waktu

Jumlah Kumulatif Persatuan Luas

(µg/cm2)

120

10,9545

3,7620

3,7957

1897,8335 287,2086

150

12,2475

4,2621

4,2997

2149,8600 325,3490

180

13,4164

4,9130

4,9556

2477,8105 374,9794

210

14,4914

5,3497

5,3988

2699,4150 408,5159

240

15,4919

6,1468

6,2003

3100,1485 469,1609

270

16,4317

6,6952

6,7567

3378,3340 511,2601

300

17,3205

7,2084

7,2754

3637,6760 550,5077

330

18,1659

8,0442

8,1163

4058,1420 614,1389

360

18,9737

8,4962

8,5766

4288,3210 648,9730

390

19,7484

8,8922

8,9772

4488,5810 679,2794

420

20,4939

9,2492

9,3381

4669,0610 706,5923

450

21,2132

9,4082

9,5007

4750,3460 718,8936

480

21,9089

10,0462

10,1403

5070,1410 767,2897

(11)

2. Tabel perhitungan Formula I (Replikasi 2)

(µg/cm2)

120

10,9545

4,2976

4,3331

2166,5580 327,8760

150

12,2474

4,4730

4,5160

2257,9880 341,7126

180

13,4164

5,5155

5,5602

2780,1150 420,7287

210

14,4914

5,9140

5,9692

2984,5775 451,6710

240

15,4919

6,3444

6,4035

3201,7700 484,5398

270

16,4317

6,8099

6,8733

3436,6720 520,0887

300

17,3205

6,7909

6,8590

3429,4995 519,0033

330

18,1659

8,1169

8,1848

4092,4045 619,3240

360

18,9737

8,3659

8,4471

4223,5345 639,1686

390

19,7484

8,6459

8,7296

4364,7795 660,5439

420

20,4939

9,2459

9,3324

4666,1795 706,1562

450

21,2132

9,3889

9,4814

4740,6795 717,4307

480

21,9089

10,6329

10,7268

5363,3945 811,6693

(12)

3. Tabel perhitungan Formula I (Replikasi 3)

(µg/cm2)

120

10,9545

3,9536

3,9867

1993,3640 301,6657

150

12,2474

4,1927

4,2322

2116,1180 320,2427

180

13,4164

5,0121

5,0540

2527,0135 382,4256

210

14,4914

5,5095

5,5596

2779,8105 420,6826

240

15,4919

6,2204

6,2755

3137,7475 474,8510

270

16,4317

6,7369

6,7991

3399,5520 514,4712

300

17,3205

7,8942

7,9616

3980,7845 602,4320

330

18,1659

8,2320

8,3109

4155,4710 628,8682

360

18,9737

8,9970

9,0793

4539,6600 687,0094

390

19,7484

8,8510

8,9410

4470,4850 676,5408

420

20,4939

9,2270

9,3155

4657,7550 704,8813

450

21,2132

9,3260

9,4183

4709,1350 712,6569

480

21,9089

10,0330

10,1263

5063,1300 766,2287

(13)

4. Tabel perhitungan Formula II (Replikasi 1)

(µg/cm2)

120

10,9545

9,2080

9,2778

4638,9100 702,0294

150

12,2474

10,1610

10,2531

5126,5400 775,8249

180

13,4164

10,9390

11,0406

5520,3050 835,4153

210

14,4914

11,5450

11,6544

5827,1950 881,8585

240

15,4919

12,3550

12,4705

6235,2250 943,6077

270

16,4317

12,7750

12,8986

6449,2750 976,0010

300

17,3205

12,9800

13,1078

6553,8750 991,8306

330

18,1659

14,2390

14,3688

7184,4000 1087,2511

360

18,9737

15,6640

15,8064

7903,1950 1196,0299

390

19,7484

15,1350

15,2916

7645,8200 1157,0801

420

20,4939

15,5840

15,7354

7867,6750 1190,6545

450

21,2132

16,0940

16,2498

8124,9200 1229,5847

480

21,9089

16,0940

16,2549

8127,4700 1229,9706

(14)

5. Tabel perhitungan Formula II (Replikasi 2)

(µg/cm2)

120

10,9545

7,1510

7,2135

3606,7300 545,8245

150

12,2474

8,3690

8,4405

4220,2550 638,6723

180

13,4164

9,1340

9,2177

4608,8450 697,4795

210

14,4914

10,1350

10,2263

5113,1700 773,8015

240

15,4919

10,8970

10,9984

5499,1750 832,2176

270

16,4317

11,4780

11,5870

5793,4850 876,7570

300

17,3205

12,0800

12,1948

6097,3900 922,7485

330

18,1659

12,5300

12,6508

6325,4000 957,2544

360

18,9737

13,5720

13,6973

6848,6500 1036,4404

420

20,4939

13,9990

14,1347

7067,3600 1069,5389

450

21,2132

15,4370

15,5770

7788,4950 1178,6718

480

21,9089

15,6350

15,7894

7894,6850 1194,7421

(15)

6. Tabel perhitungan Formula II (Replikasi 3)

(µg/cm2)

120

10,9545

9,3190

9,3990

4699,4950 711,1980

150

12,2474

10,4160

10,5092

5254,5950 795,2041

180

13,4164

11,3430

11,4472

5723,5800 866,1779

210

14,4914

12,2360

12,3494

6174,7150 934,4504

240

15,4919

12,6820

12,8044

6402,1800 968,8739

270

16,4317

13,6260

13,7528

6876,4100 1040,6414

300

17,3205

14,0660

14,2023

7101,1300 1074,6494

330

18,1659

14,3470

14,4877

7243,8300 1096,2449

360

18,9737

15,4180

15,5615

7780,7350 1177,4975

390

19,7484

15,6790

15,8332

7916,5900 1198,0571

420

20,4939

16,3010

16,4578

8228,8950 1245,3197

450

21,2132

16,6800

16,8430

8421,5050 1274,4684

480

21,9089

17,3310

8665,5000 1311,3934

(16)

LAMPIRAN 7

Hasil Pengolahan Statistik dengan SPSS

1. Hasil analisis statistik

independent sample t-test

pH sediaan gel natrium

diklofenak

Independent Samples Test

Levene's Test for

Equality of

Variances t-test for Equality of Means

95% Confidence Interval of

the Difference

F Sig. t df

Sig.

(2-tailed)

Mean

Difference

Std. Error

Difference _Lower _Upper

Equal

variances

assumed

2.632 .180 1.591 4 .187 .03000 .01886 -.02235 .08235 pH

Equal

variances not

assumed

1.591 3.039 .209 .03000 .01886 -.02958 .08958 Group Statistics

Formula N Mean Std. Deviation Std. Error Mean

Mikro 3 5.9967 .01528 .00882 pH

(17)

2. Hasil analisis statistik

independent sample t-test

daya sebar sediaan gel

natrium diklofenak

Levene's Test for

Equality of

95% Confidence Interval of

the Difference

F Sig. T df

Sig.

(2-tailed)

Mean

Difference

Std. Error

Difference _Lower _Upper

Equal

variances

assumed

2.574 .184 -6.159 4 .004 -.0361333 .0058667 -.0524218 -.0198449 DS

Equal

variances

not assumed

-6.159 2.130 .022 -.0361333 .0058667 -.0599552 -.0123114 Group Statistics

mikro 3 .011867 .0018037 .0010414 DS

(18)

3. Hasil analisis statistik

independent sample t-test

diameter pada beban nol

sediaan gel natrium diklofenak

Levene's Test

for Equality of

95% Confidence Interval

of the Difference

F Sig. t df Sig.

(2-tailed)

Mean

Difference

Std. Error

Difference Lower Upper

Equal

variances

assumed

1.166 .341 -9.395 4 .001 -6.33333 .67412 -8.20500 -4.46166 Bebanol

Equal

variances not

assumed

-9.395 2.868 .003 -6.33333 .67412 -8.53579 -4.13088 Group Statistics

mikro 3 4.9667 .50332 .29059 Bebanol

(19)

5. Hasil Analisis statistik

independent sampel t test

fluks sediaan natrium

diklofenak

Group Statistics

Mikro 3 37.118467 .2828274 .1632905 Fluks

emulsi 3 59.695667 1.9467765 1.1239719

Levene's

Test for

Equality of

95% Confidence Interval of the

Difference

F Sig. t df Sig.

(2-taile

d)

Mean

Difference

Std.

Error

Differen

ce Lower Upper

Equal

variances

assumed

8.894 .041 -19.878 4 .000 -22.5772000 1.13577

14

-25.7306069 -19.4237931 Fluks

Equal

variances

not assumed

-19.878 2.084 .002 -22.5772000 1.13577

14

(20)

LAMPIRAN 8

Tabel Distribusi r

Degrees of

Freedom

(DF)

5 Percent

1 Percent

Degrees of

Freedom

5 Percent

1 Percent

1 .997 1.000

24 .388 .496

2 .950 .990

25 .381 .487

3 .878 .959

26 .374 .478

4 .811 .917

27 .367 .470

5 .754 .874

28 .361 .463

6 .707 .834

29 .355 .456

7 .666 .798

30 .349 .449

8 .632 .765

35 .325 .418

9 .602 .735

40 .304 .393

10 .576 .708

48 .288 .372

11 .553 .684

50 .273 .354

12 .532 .661

60 .250 .325

13 .514 .641

70 .232 .302

14 .497 .623

80 .217 .283

15 .482 .606

90 .205 .267

16 .468 .590

100 .195 .254

17 .456 .575

125 .174 .228

18 .444 .561

150 .159 .208

19 .433 .549

200 .138 .181

20 .423 .537

300 .113 .148

21 .413 .526

400 .098 .125

22 .404 .515

500 .088 .115

(21)