Bovine Serum Albumin (BSA) sebanyak 100 mg dilarutkan kedalam 100 ml ddH2O

Teks penuh

(1)

54

Lampiran 1. Kurva Standar Protein

Pembuatan Reagen Bradford

Commasive blue

sebanyak 0,01 gram dilarutkan kedalam 50 ml etanol 95% dan

ditambah dengan asam fosfor 85% sebanyak 100 ml. Larutan yang digunakan

diencerkan dengan menggunakan ddH

2

O hingga 1000 ml. Larutan tersebut kemudian di

saring dan disimpan dalam botol gelap pada suhu rendah (Bollag & Stuart, 1991).

Pembuatan Kurva Standar Protein

Bovine Serum Albumin

(BSA) sebanyak 100 mg dilarutkan kedalam 100 ml ddH

2

O

sehingga diperoleh larutan stok BSA 1 mg/ml. Selanjutnya dibuat seri pengenceran

larutan standar dengan konsentrasi 0 mg/ml; 0,1 mg/ml; 0,2 mg/ml; 0,3 mg/ml; 0,4

mg/ml; 0,5 mg/ml; 0,6 mg/ml; 0,7 mg/ml; 0,8 mg/ml; 0,9 mg/ml; dan 1 mg/ml. Dari

masing-masing konsentrasi diambil 0,1 ml larutan standar dan ditambahkan 5 ml larutan

Bradford. Kemudian didiamkan selama 30 menit dan diukur absorbansinya pada λ 595

(2)

Lampiran 2. Komposisi Reagen Analisis Molekuler

Nama Larutan

Komposisi

Larutan A

(

Acrylamide Stock Solution

), 100

ml 30%

acrylamide

, 0,8%

bis-acrylamide

29,2 gram

acrylamide

0,8 gram

bis-acrylamide

Larutan B

(4x Separating Gel Buffer), 100 ml

75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M

4 ml 10% SDS --- 0,4 %

21 ml ddH

2

O

Larutan C

(4x Stacking Gel Buffer), 100 ml

50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M

4 ml 10% SDS --- 0,4 %

46 ml ddH

2

O

10%

Ammonium Persulphate

(APS)

0,5 gram APS

5 ml DDH

2

O

Buffer Elektroforesis

3 gr Tris --- 25Mm

14,4 gram

glycine

--- 192Mm

1 gram SDS --- 0,1%

ddH

2

O sampai 1 liter

Sampel Buffer

3,028 gram Tris

0,25 ml larutan C

0,4 ml 10% SDS

25 ml gliserol

0,2 mg

bromophenol blue

0,031 gram

Dithiothreitol

(DTT)

Lampiran 3. Kurva Standar BSA (

Bovine Serum Albumin

)

Low Molecular Weight

y = -0,985x + 1,803

R² = 0,950

-0,200

0,000

0,200

0,400

0,600

0,800

1,000

0,000 0,500 1,000 1,500 2,000

Series1

(3)

Lampiran 4. Berat Molekul Fraksi Protein Glutenin Adonan Tepung Terigu Protein

Tinggi yang Mengandung Kayu Manis

Konsentrasi

Rf

Berat Molekul (kDa)

0% kayu manis (tanpa

kayu manis)

0,04

Lampiran 5. Analisis Data Hasil Penelitian Adonan tepung terigu yang mengandung

Kayu Manis

hardness

Duncana

6 207,6350

6 288,6367

6 363,6717

1,000 1,000 1,000 konsentrasi

kontrol

kayu manis 1,5% kayu manis 3% Sig.

N 1 2 3

Subset for alpha = .05

(4)

Lampiran 6. Hasil Pengolahan SPSS Roti Kukus yang Mengandung Kayu Manis

Analisis Tingkat Kekerasan (

Hardness

)

Tanpa Penambahan Kayu Manis (Kontrol)

Penambahan Kayu Manis Konsentrasi 1,5%

hardness

Duncana,b

6 118,8150

6 180,1733

6 386,8167

1,000 1,000 1,000

perlakuan proofing mixing steaming Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 119,757. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

hardness

Duncana,b

6 174,4100

6 234,3617

6 1225,8167

,164 1,000

perlakuan proofing mixing steaming Sig.

N 1 2

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 5046,095. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

hardness_kontrol

(5)

Penambahan Kayu Manis Konsentrasi 3%

Tahapan

Mixing

Tahapan

Proofing

hardness

Duncana,b

6 252,2850

6 358,7067

6 1614,6833

1,000 1,000 1,000

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 1549,066. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

hardness

Duncana,b

6 180,1733

6 234,3617

6 358,7067

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 84,606. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

hardness

Duncana,b

6 118,8150

6 174,4100

6 252,2850

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 631,594. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

(6)

Tahapan

Steaming

Analisis Warna

Tingkat Kecerahan/

Lightness

(L*)

Nilai a*

hardness

Duncana,b

6 386,8167

6 1225,8167

6 1614,6833

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 5998,717. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

L

Duncana

6 62,4533

6 66,8917

6 84,2567

1,000 1,000 1,000

konsentrasi kons 3% kons 1,5% Kontrol Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 6,000. a.

a

Duncana

6 -2,9100

6 5,1633

6 8,9817

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 6,000. a.

(7)

Nilai b*

Analisis Volume Pengembangan

Tanpa Penambahan Kayu Manis (Kontrol)

Penambahan Kayu Manis Konsentrasi 1,5%

b

Duncana

6 21,0550

6 25,2833

6 28,1817

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 6,000. a.

volume

Duncana,b

6 32,1687

6 50,1483

6 84,3860

1,000 1,000 1,000

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,284. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume

Duncana,b

6 31,3758

6 45,4053

6 69,9873

1,000 1,000 1,000

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 3,883. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume_kontrol

(8)

Penambahan Kayu Manis 3%

Tahapan

Mixing

volume

Duncana,b

6 31,1778

6 42,1075

6 63,7912

1,000 1,000 1,000

perlakuan mixing proofing steaming Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,102. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume

Duncana,b

6 32,1687

6 50,1483

6 84,3860

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,284. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume_3%

(9)

Tahapan

Proofing

Tahapan

Steaming

volume

Duncana,b

6 31,3758

6 45,4053

6 69,9873

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 4,080. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume

Duncana,b

6 31,1778

6 42,1075

6 63,7912

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,102. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

volume_proofing

(10)

Aktivitas Antioksidan

Tanpa Penambahan Kayu Manis (Kontrol)

Penambahan Kayu Manis Konsentrasi 1,5%

antioksidan

Duncana,b

6 4,4567

6 6,8017

6 7,9583

1,000 ,051

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = ,888. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

antioksidan

Duncana,b,c

5 20,0580

6 21,0150 21,0150

6 22,3033

,272 ,146

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 1,967. Uses Harmonic Mean Sample Size = 5,625. a.

The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.

b.

Alpha = ,05. c.

antioksidan_kontrol

(11)

Penambahan Kayu Manis 3%

Tahapan

Mixing

Tahapan

Proofing

antioksidan

Duncana,b

6 33,0867

6 33,9183

6 36,2167

,432 1,000

perlakuan

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 3,176. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

antioksidan

Duncana,b

6 4,4567

6 19,7500

6 33,0867

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 1,551. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

antioksidan

Duncana,b

6 7,9583

6 22,3033

6 36,2167

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,335. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

antioksidan_3%

antioksidan_mixing

(12)

Tahapan

Steaming

antioksidan

Duncana,b

6 6,8017

6 21,0150

6 33,9183

1,000 1,000 1,000

konsentrasi kontrol kons 1,5% kons 3% Sig.

N 1 2 3

Subset

Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares

The error term is Mean Square(Error) = 2,203. Uses Harmonic Mean Sample Size = 6,000. a.

Alpha = ,05. b.

Figur

Memperbarui...

Referensi

Memperbarui...