macrophage phagocytosis

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this PDF file Correlation Between Total Flavonoid Contents and Macrophage Phagocytosis Activity of Fractions From Faloak (Sterculia quadrifida R.Br.) Barks Ethanolic Extract In Vitro | Munawaroh | Majalah Obat Tradisional (Traditional Medicine Journal) 1

this PDF file Correlation Between Total Flavonoid Contents and Macrophage Phagocytosis Activity of Fractions From Faloak (Sterculia quadrifida R.Br.) Barks Ethanolic Extract In Vitro | Munawaroh | Majalah Obat Tradisional (Traditional Medicine Journal) 1

On Timor island, Nusa Tenggara Timur, faloak barks (Sterculia quadrifida R.Br.) has been used empirically to restore stamina. Faloak bark ethanolic extract proved to have immunomodulatory activity in vitro, which can increase macrophage phagocytosis activity. This research aimed: (i) to determine the immunomodulatory active fraction of faloak bark ethanolic extract, (ii) to determine the total flavonoid contents of faloak extract and fractions, and (iii) to evaluate the correlation of the total flavonoid contents of those extract and fractions with their macrophage phagocytosis activity. The simplisia powder is macerated with 96% ethanol. The extract was dissolved in methanol:water (9:1v/v) was then subsequently partitioned with n-hexane, ethyl acetate, and water to obtain n-hexane fraction, ethyl acetate fraction, water fraction, and insoluble fraction. Faloak extract and fractions at concentration , ; ; ; μg/mL were tested for their effect on the peritoneal macrophage phagocytosis of Balb/c mice in vitro by the latex beads method. Phagocytosis capacity and phagocytosis index were analyzed using one-way anova and post hoc Tukey HSD test with 95% confidence level. The results showed that ethyl acetate fraction had the highest macrophage phagocytosis capacity and the highest total flavonoid content compared to other fractions. The highest macrophage phagocytosis capacity of ethyl acetate fraction at concentration of μg/mL was 51,94±4,67%, this value was significantly different from cell control (7,50±1,29%), negative controls of 0,0625% dimethylsul phoxide , ± , % , as well as positive control of μg/mL echinaceae extract syrup® (9,97±0,33%). The total flavonoid content of ethyl acetate fraction determined by aluminum chloride method was 4,290±0.029 mg of quercetin equivalent/g fraction. There was a positive and strong correlation between the total flavonoid content of these extract and fractions with their macrophage phagocytosis capacity (Pearson correlation coefficient of 0,781) and showing linear relationship y=4,721x+19,663; R 2 =0,61.
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The Effect of Tempeh on the Ability of Macrophage Phagocytosis

The Effect of Tempeh on the Ability of Macrophage Phagocytosis

Latar belakang: Konsumsi tempe secara teratur dapat meningkatan kadar isoflavon dalam tubuh yang mampu mempengaruhi IFNγ dan Macrophage Activating Factor (MAF) sehingga menyebabkan peningkatan kemampuan fagositosis makrofag. Fagositosis makrofag memberi gambaran mengenai kemampuan makrofag sebagai sel fagosit lini pertama untuk mencerna partikel asing. Tujuan penelitain ini untuk mengetahui pengaruh tempe terhadap kemampuan fagositosis makrofag.

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UJI AKTIVITAS FAGOSITOSIS MAKROFAG SENYAWA KODE PC-2 DARI DAUN SIRIH MERAH (Piper crocatum Ruiz Pav.) SECARA IN-VIVO In vivo activity study of macrophage phagocytosis by Pc-2 compound of Daun Sirih Merah (Piper crocatum Ruiz Pav.)

UJI AKTIVITAS FAGOSITOSIS MAKROFAG SENYAWA KODE PC-2 DARI DAUN SIRIH MERAH (Piper crocatum Ruiz Pav.) SECARA IN-VIVO In vivo activity study of macrophage phagocytosis by Pc-2 compound of Daun Sirih Merah (Piper crocatum Ruiz Pav.)

activity. The isolation of Pc-2 compound of methanolic extract of red betel leaf have been done and was test- ed in vivo on macrophage phagocytic activity. Methanolic extract of red betel leaf is obtained by maceration, fractionated by vacuum liquid chromatography method, and compound code Pc-2 was isolated by prepara- tive thin layer chromatography method. The content of the compounds in the extract, fractions, and isolates were monitored by thin layer chromatography. Macrophage phagocytic activity assays using BALB/c mice in- duced with Lysteria monocytogenes bacteria. Phagocytic activity expressed in percentage of phagocytosis (PF), phagocytic index (IF), and the effectiveness of phagocytosis (EF). The results showed that mice treated with the compound code Pc-2 dose of 10 mg/kg both at day3rd, 10th, and 21st after infection with L. monocytogenes have PF, IF, and EF that significantly greater than the controlgroup mice; macrophage phagocytic activity oc- curred on day 21 after infection with L. monocytogenes, and there is no correlation between the PF and IF.
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Pengaruh Pemberian Vitamin D Terhadap Perbaikan Foto Toraks Pada Pasien TB Paru Etnik Batak Di Kota Medan Dihubungkan Dengan Polimorfisme ApaI Gen Reseptor Vitamin D

Pengaruh Pemberian Vitamin D Terhadap Perbaikan Foto Toraks Pada Pasien TB Paru Etnik Batak Di Kota Medan Dihubungkan Dengan Polimorfisme ApaI Gen Reseptor Vitamin D

Selvaraj P, Candra G, Jawahar MS, Rani MV, Rajeshwari DN, Narayanan PR. Regulatory role of vitamin D reseptor gen variants of BsmI, ApaI, TaqI, and FokI polymorphisms on macrophage phagocytosis and lymphoproliferative response to Mycobacterium tuberculosis antigen in pulmonary tuberculosis . J Clin Immunol 2004;24:523-32

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Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA induced rats

Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA induced rats

Aside from thymoquinone, some unsaturated fatty acids such as sitosterol and terpenoids have also shown to increase the activity of macrophage phagocytosis and ROI secretion, which was exposed by xenobiotic. This is done through a cytoprotective mechanism by increasing the secretion of glutathione antioxidant by glutathione S-transferase (GST) due to the activation of the antioxidant responsive element (ARE). Results showed that the hexane extracts of black cumin seeds suppressed the activity of CYP genes and stimulated the activity of GST genes and enzymes.
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In-vivo immunomodulatory and histophatological effect of two compounds isolated from red betel (Piper crocatum Ruiz & Pav.).

In-vivo immunomodulatory and histophatological effect of two compounds isolated from red betel (Piper crocatum Ruiz & Pav.).

Red betel (Piper crocatum Ruiz & Pav) leaves have been used traditionally by Indonesian People to maintain their health. Previous study of the leaves indicated that the leaves displayed immunomodulatory activity in vitro (macrophage phagocytosis test). Two principal active substances were isolated from the MeoH Extract, identified as a neolignan (Pc-1) compound and its deacetyl derivative (Pc-2). This recent study we report histophatological effect and in vivo immunomodulatory effect on mice treated with Pc-1 and Pc-2.

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Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA inducedrats.

Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA inducedrats.

Aside from thymoquinone, some unsaturated fatty acids such as sitosterol and terpenoids have also shown to increase the activity of macrophage phagocytosis and ROI secretion, which was exposed by xenobiotic. This is done through a cytoprotective mechanism by increasing the secretion of glutathione antioxidant by glutathione S-transferase (GST) due to the activation of the antioxidant responsive element (ARE). Results showed that the hexane extracts of black cumin seeds suppressed the activity of CYP genes and stimulated the activity of GST genes and enzymes.
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PROS Retno Sri I, R. Susanti, M Dafip Vitamin A induction fulltext

PROS Retno Sri I, R. Susanti, M Dafip Vitamin A induction fulltext

study tested the distribution of data is the Kolmogorov-Smirnov and showed normal distribution. Obtained measurement results indicate a normal distribution so that hypothesis testing the levels of macrophage phagocytosis index using One-way ANOVA test followed by Least Significant Difference (LSD). ROI and NOI production was used nonparametric test considering ROI and NOI calculation data in ordinal scale, ie the nonparametric Kruskal Wallis test followed by Mann Whitney U test statistical analysis assisted with SPSS 13 for windows. Significant value in this research when variables analyzed had p < 0.05.
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Directory UMM :Data Elmu:jurnal:A:Animal Reproduction Science:Vol64.Issue3-4.Dec2000:

Directory UMM :Data Elmu:jurnal:A:Animal Reproduction Science:Vol64.Issue3-4.Dec2000:

PDGF increased DNA synthesis by porcine granulosa cells (Hammond and English, 1987) and was mitogenic to porcine theca cells collected from small (1–4 mm) follicles (May et al., 1992). PDGF also stimulated porcine theca cell proliferation and activated kinase signalling pathways in a culture system containing serum (Taylor, 2000). Recent work using rat theca-interstitial cells showed 30–136% increases in DNA synthesis with PDGF doses of 3–30 ng/ml (Duleba et al., 1999). Lack of IGF-1 was previously found to be detrimental to the number of viable theca cells (Shores et al., 2000). PDGF was therefore, able to compensate to some extent for the lack of IGF-1 by increasing viable cell number when a dose of 10 ng/ml was used. In the presence of IGF-1, PDGF treatment increased viable cell number but doses of 1 and 10 ng/ml were not different. This is in agreement with Duleba et al. (1999) who observed additive effects of PDGF and IGF-1 on rat theca cell DNA synthesis. Macrophage removal increased viable cell number at 144 h and therefore, a macrophage product suppressed viable cell number. PDGF cannot mediate this theca cell/macrophage interaction since PDGF increased viable cell number.
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Immunomodulator of Ethanol Extracts of The Leaves Azadirachta indica Against Macrophage Peritoneal Cell in Mice Induced The Vaccine BCG

Immunomodulator of Ethanol Extracts of The Leaves Azadirachta indica Against Macrophage Peritoneal Cell in Mice Induced The Vaccine BCG

Menurut penelitian Susanti dkk, tentang karakterisasi kultur makrofag dari peritoneum macrophage (PMs) menunjukkan makrofag lebih matur dibandingkan Bone marrow macrophage (BMs) dan Spleenic macrophage (SPMs). 5 Jumlah rata-rata makrofag peritoneal pada kelompok kontrol negatif (K(-)) adalah 80 makrofag/mm 3 . Jumlah makrofag pada kontrol negatif sesuai dengan nilai rujukan hitung sel monosit atau makrofag yaitu 2% dari sel lekosit yaitu 80 sel/mm 3 . Hasil tersebut lebih rendah dibandingkan dengan kelompok kontrol positif (K(+)) yaitu kelompok mencit yang diinduksi vaksin BCG tanpa perlakuan pemberian ekstrak etanol daun mimba dengan rata-rata jumlah makrofag peritoneal sebesar 380 sel/mm 3 . Pada kelompok positif menunjukkan adanya aktivitas makrofag akibat imunogenik dari vaksin BCG. Adanya antigen vaksin BCG menyebabkan terjadinya makrofag melakukan proliferasi dan fagositosis. 10 Adanya mencit dengan jumlah makrofagnya 250 sel/mm 3 , lebih kecil dari mencit yang lain pada kelompok positif, kemungkinan disebabkan ekspresi sitokin oleh antigen Mycobacterium bovis tidak maksimal sehingga berpengaruh terhadap jumlah makrofag. 11 Jumlah makrofag peritoneal pada kelompok P1, P2 dan P3 yaitu kelompok mencit yang diiinduksi vaksin BCG dan diberi perlakuan pemberian ekstrak etanol daun mimba dengan dosis 200 mg/Kg BB selama 2 hari, 4 hari, dan 6 hari cenderung menurun jumlahnya apabila dibandingkan dengan kelompok kontrol positif.
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Directory UMM :Data Elmu:jurnal:A:Atherosclerosis:Vol154.Issue1.Jan2001:

Directory UMM :Data Elmu:jurnal:A:Atherosclerosis:Vol154.Issue1.Jan2001:

In apoE+ / +.LDLr − / − mice, apoE-containing lipoproteins can still bind to LRP, proteoglycans, and the putative remnant receptor, and hence no dramatic accumulation of lipoprotein remnants occurs [15]. In contrast, in apoE− / −.LDLr + / + mice, apoE, a high affinity ligand for several binding sites in the liver, is absent, resulting in a profound accumulation of lipoprotein remnants [8 – 11]. Reconstitution of macrophage apoE production rescued these apoE− / −.LDLr + / + mice from hypercholesterolemia and development of premature atherosclerosis [25 – 28]. Re- constitution of macrophage apoE production in apoE− / − .LDLr− / − mice, however, induced only a small decrease in serum VLDL cholesterol levels, despite normal serum apoE concentrations. It can be postulated that the decreased ability of macrophage- derived apoE to reduce serum cholesterol levels in the absence of the LDL receptor is either due to insuffi- cient levels of apoE on lipoprotein remnant particles or by the necessity of production of apoE by par- enchymal liver cells to trigger uptake by non-LDL receptor pathways, including LRP. In Table 1, in which a summary is given of the data obtained in this study, data of a previous study by our group, per- formed under the same conditions [29] are also in- cluded, thereby clearly demonstrating the effects of macrophage versus hepatic LDL receptor expression and apoE synthesis on serum cholesterol levels. Comparison of our recent data with the previously published data [29] reveals that in apoE+ / + .LDLr− / − “ apoE+ / +.LDLr − / − transplanted mice with apoE production by liver, macrophages, and other extrahepatic tissues, serum cholesterol levels are
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Directory UMM :Data Elmu:jurnal:A:Atherosclerosis:Vol152.Issue1.Sep2000:

Directory UMM :Data Elmu:jurnal:A:Atherosclerosis:Vol152.Issue1.Sep2000:

During atherogenesis, a pathological accumulation of lipids occurs within aortic intimal macrophages through uptake of oxidised low-density lipoprotein (LDL) via scavenger receptors. Here we investigate whether some of the anti-atherosclerotic effects ascribed to dietary fish oil are mediated through effects on macrophage intercellular adhesion molecule 1 (ICAM-1) and scavenger receptor expression. Mice were fed on a low fat diet (containing 25 g/kg corn oil) or on high fat diets containing 200 g/kg coconut oil, safflower oil or fish oil. Thioglycollate-elicited peritoneal macrophages were analysed for fatty acid composition by gas chromatography. Macrophage scavenger receptor A (MSR-A) type I + type II and ICAM-1 expression were measured by flow cytometry and the levels of mRNA coding for MSR-A type I, MSR-A type II and ICAM-1 were measured by reverse-transcription polymerase chain reaction. Feeding mice diets enriched with different fats resulted in significant changes in the fatty acid profile of macrophages, which reflected the fatty acid compositions of the diets. Macrophages from the fish oil fed mice had the lowest expression of ICAM-1 and MSR-A at the level of both mRNA and cell surface expression. The reduced expression of ICAM-1 and MSR-A on macrophages from mice fed on a fish oil-rich diet supports our hypothesis that part of the protective effect of fish oil against atherosclerosis might be due to an altered macrophage phenotype and function ameliorating macrophage-induced plaque formation. © 2000 Elsevier Science Ireland Ltd. All rights reserved.
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