optimization of fermentation medium

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Optimization of Streptomyces sp.A11 Medium Cultivation on Cyclo(Tyrosyl-Prolyl) Production Using The Response Surface Methodology - Diponegoro University | Institutional Repository (UNDIP-IR) BRE 02 Rofiq S

Optimization of Streptomyces sp.A11 Medium Cultivation on Cyclo(Tyrosyl-Prolyl) Production Using The Response Surface Methodology - Diponegoro University | Institutional Repository (UNDIP-IR) BRE 02 Rofiq S

The response surface methodology is a three factorial design which provides the relationship between one or more measured dependent response and a number of input (independent) factors. Response surface methodology was used for optimization of fermentation medium for antibacterial agent cyclo(tyr-pro) production by Streptomyces sp A11. The optimal response region of the significant factor was predicted by using a second order polynomial model fitted to the results obtained by applying the Central Composite Design (CCD) statistical design.
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Isolation, purification, identification, and fermentation medium optimization of antibiotic produced by marine actinomycetes

Isolation, purification, identification, and fermentation medium optimization of antibiotic produced by marine actinomycetes

Pengamatan nilai pH medium fermentasi dapat digunakan untuk mengetahui adanya aktivitas pertumbuhan sel. Apabila dilihat dari profil perubahan pH, terlihat bahwa dari jam ke-0 sampai dengan jam ke-45 terjadi penurunan pH seiring dengan penurunan konsentrasi gula. Hal ini disebabkan terjadinya hidrolisis gula yang diubah menjadi asam-asam organik yang menyebabkan suasana medium fermentasi menjadi asam. Dengan demikian secara tidak langsung bahwa penurunan pH menunjukkan adanya konversi substrat menjadi senyawa lain seperti asam organik, dan protein. Secara umum penurunan pH bersamaan dengan penurunan konsentrasi gula. Pengamatan pH setelah jam ke-45 terjadi kenaikan pH pada medium fermentasi, hal ini disebabkan oleh terjadinya deaminasi protein yang dapat menyebabkan kondisi kaldu fermentasi menjadi lebih basa. Menurut Wang et al. (1979) penggunaan sumber nitrogen organik cenderung memicu naiknya pH fermentasi yang disebabkan oleh terjadinya deaminasi asam amino. Lisis sel atau rusaknya sebagian sel dalam medium fermentasi juga dapat mempengaruhi kenaikan pH medium fermentasi. Sel disusun oleh beberapa protein organik, apabila terjadi kerusakan sel maka terjadi deaminasi asam amino yang mengakibatkan naiknya pH kaldu fermentasi. Kisaran pH selama proses fermentasi terlihat pada kisaran pH 5,8-7,6. Nilai pH ini masih pada batas toleransi aktinomisetes pada umumnya. Menurut Goodfellow et al. (1988) aktinomisetes mampu tumbuh baik pada kisaran pH 6-8. Actinohordin yang dihasilkan oleh Streptomyces coelicolor A3(2) memiliki kisaran pH 7,2-8. Seperti halnya Streptomyces sp. A11, Streptomyces coelicolor A3(2) memiliki profil pH yang mirip dengan Streptomyces sp. A11. Pada fase eksponensial terjadi penurunan pH sampai mendekati fase stasioner, selanjutnya terjadi kenaikan pH sampai dengan pH 8 sampai akhir fermentasi (Ulgen dan Mavituna 1993).
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OPTIMIZATION OF BACTERIOCIN PRODUCTION BY LACTIC ACID BACTERIA ISOLATED FROM AMPEL BAMBOO SHOOTS (Bambusa vulgaris) PICKLE UNDER DIFFERENT FERMENTATION CONDITIONS USING SUPPLEMENTED WHEY MEDIUM OPTIMASI PRODUKSI BAKTERIOSIN BAKTERI ASAM LAKTAT YANG DIISOL

OPTIMIZATION OF BACTERIOCIN PRODUCTION BY LACTIC ACID BACTERIA ISOLATED FROM AMPEL BAMBOO SHOOTS (Bambusa vulgaris) PICKLE UNDER DIFFERENT FERMENTATION CONDITIONS USING SUPPLEMENTED WHEY MEDIUM OPTIMASI PRODUKSI BAKTERIOSIN BAKTERI ASAM LAKTAT YANG DIISOL

If it is proven in the future that partially or whole thesis is the result of plagiarism, therefore I am willing to be revoked with all the consequences in accordance with the law and regulations applied at Soegijapranata Catholic University and/or valid law and regulations.

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OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium).

OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium).

Hasil uji optimasi sumber nitrogen (Gambar 3) menunjukkan bahwa isolat SLK1 lebih optimum untuk tumbuh dan memproduksi selulosa pada sumber nitrogen dengan kadar nitrogen tinggi (Ammonium sulfat dan ZA) dibandingkan sumber N dengan kadar N lebih rendah seperti urea dan pepton. Isolat SLK1 mencapai angka produksi selulosa tertinggi pada sumber nitrogen ammonium sulfat sebesar 0.34 g/100 ml, diikuti ZA sebesar 0.31 g/100 ml, urea 0.18 g/100 ml dan paling rendah pepton sebesar 0.16 g/100 ml. Secara umum penambahan sumber N sangat penting dalam medium fermentasi selulosa utamanya untuk memacu pertumbuhan sel bakteri asam asetat (Kurosumi et al, 2009).
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Network Optimization Of IKN.

Network Optimization Of IKN.

To do this research, it requires a hardware and also software that support analysis features for that network. Every element of this network will be collected and study so the researcher can find the weakness of that network. The limitation of this project is to gather data with the company where the location are far from the researcher location and researcher study schedule full with class and hard to collect data in weekdays.

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Handbook of Yoghurt Science and Technology

Handbook of Yoghurt Science and Technology

Due to the taste and health benefits, yoghurt soon became popular in the Western world. In the early 1500s, King Francis I of France was reportedly cured of a debilitating illness after eating yoghurt made from goats’ milk. Scientific interest in the health benefits of yoghurt was initiated by Élie Metchnikoff in the early 1900s (Metchnikoff, 1908). Metchnikoff proposed that the lactic acid microbes of fermentation must be antagonistic to the putrefying microbes of the gut, and once introduced into the intestine, they would prevent the breeding of the noxious microbes that required an alkaline environment. His hypothesis was stimulated by the fact that populations such as those living in the Balkans regularly ate yoghurt and were noted for their longevity.
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Computer Network Reliability Optimization Calculation Based on Genetic Algorithm

Computer Network Reliability Optimization Calculation Based on Genetic Algorithm

Genetic algorithm is used to simulate the process of data transmission by using biological natural selection and genetic mechanism. Establish the optimized information search function suitable for the whole network through population search and information exchange between individuals. It requires easy operation, comprehensive functions and outstanding advantage, which is suitable for the whole network, so as to solve the problem of data transmission. When using genetic algorithm to conduct optimization calculation of the network reliability, firstly, it is required to establish genetic algorithm solution, take sequence as the chromosome of coding, and elements composed of the coding as the gene of genetic algorithm, so as to form the initial population of a set of chromosomes. Finally, produce optimized individuals which meet the stop standard through fitness operation, selection operation, crossover operation, mutation operation and other operations, so as to be suitable for the backbone network data transmission design with the highest comprehensive degree.
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application of ammoniation fermentation technology based

application of ammoniation fermentation technology based

Using urea 4% (w/w) and EM-4 8% (v/v) of palm fiber. Urea mixed with palm fiber and then is sprayed by using EM-4 solution. Put the mixture into plastic bags and than compact so that an anerob condition could be achieved. After that, the plastic bag is tied and stored at room temperature for 21 days. After 21 days, the plastic bag is opened and ammoniation-fermentation of palm fiber are ready given to buffalo. Composition of feed rations substances that used on research shown on Table 1.

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Isolation of Rhizopus oryzae From Rotten Fruit and Its Potency For Lactic Acid Production in Glucose Medium with and without Addition of Calcium Carbonate | Kurniawati | Agritech 9507 17592 1 PB

Isolation of Rhizopus oryzae From Rotten Fruit and Its Potency For Lactic Acid Production in Glucose Medium with and without Addition of Calcium Carbonate | Kurniawati | Agritech 9507 17592 1 PB

Lactic acid production in glucose medium (without CaCO 3 ) showed lower lactic acid concentration, compared to glucose+CaCO 3 medium. pH of medium might play important role on it because it affected lactic acid production strongly, due to its role in activating enzyme in R. oryzae metabolism. Initial pH of glucose medium was 4.2 and decreased to 2.7 in 1 day fermentation then slightly decreased at 2 until 5 days fermentation (pH=2.23 at day 5). This acidic medium did not support R. oryzae to grow well, also LDH to optimally convert pyruvate into lactate. Pyruvate was utilized by PDC enzyme better than LDH enzyme in this condition, so that lactic acid was produced slowly, as shown by its productivity which tended to decrease (Table 2). This slow productivity also related to inhibition activity which was mentioned bySkory (2003) that at pH near or below pK of lactic acid (pK=3.8), inhibition on lactic acid production may occur. Highest lactic acid concentration in glucose medium was reached in 4 days fermentation (11.61 g/l), then decreased at day 5 (7.83 g/l). It might be caused by reversible reaction between pyruvate and lactate, catalyzed by LDH enzyme (Moore and Landeckerin Hidayat, 2006). In this acidic environment, PDC enzyme activity was higher than LDH enzyme activity, causing ethanol produced better than lactate. Lactate which had been formed tended to reverse back into pyruvate then utilized by PDC to form ethanol.
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Cultivation Medium Selection and Optimization Agitation Speed of Bacterial Cellulose Production by Acetobacter pasteurianum in Shaking Culture

Cultivation Medium Selection and Optimization Agitation Speed of Bacterial Cellulose Production by Acetobacter pasteurianum in Shaking Culture

speed obtained from second research step. Analysis that caried out in ' this step comprised of yield of dried pure bacterial cellulose, optical density of culture medium, weight of dried biomass and rest of sugar concenraion. The analysis was carried out in ten day. First day was every 4 hour. Second until forth day was every 6 hour. Fifth day s every 8 hour and sixth until tenth day s every 12 day.

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THE UTILIZATION OF CANNA STARCH (Canna edulis Ker.) AS AN ALTERNATIVE HYDROCOLLOID ON THE MANUFACTURING PROCESS OF YOGURT DRINK

THE UTILIZATION OF CANNA STARCH (Canna edulis Ker.) AS AN ALTERNATIVE HYDROCOLLOID ON THE MANUFACTURING PROCESS OF YOGURT DRINK

Starch is a complex food hydrocolloid, polymer of α -D-glucose and partially crystalline polymer. Starch granules absorb water resulting in swelling up to several times of their original size and losing their crystallinity. The complete process is known as gelatinization. Gelatinization of starch involves changes in amylase and amylopectin (Ahmed et al., 2008). Indonesian Canna starch contained 24.06% amylose and 63.27% amylopectin that has a very high capability of water holding capacity as to increase the viscosity and maintain the stability of yogurt (Carolina and Ilmi, 2016). Recently, Canna edulis Ker as local Indonesian food only processed into several products such as vermicelli noodles, cookies, crackers, and traditional product including cendol, porridge, and biscuits. Therefore, the objectives of this study were to investigate the effect of Canna starch (Canna edulis Ker) addition on the physicochemical and sensory properties of the yogurt drink.
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A Self-Adaptive Chaos Particle Swarm Optimization Algorithm

A Self-Adaptive Chaos Particle Swarm Optimization Algorithm

In practical applications, since the initialization of the basic PSO is random, it has certain blindness. Although the random initialization can basically guarantee the uniform distribution of the initial population, it can’t guarantee the quality of every particle and it may cause some particles get far away from the optimal solution and affect the convergence speed of the algorithm [2]. PSO can easily get trapped in local extremum and it can’t have global optimal solution. The convergence speed of PSO is quite slow. It usually takes some time to reach the corresponding accuracy in solving practical problems. Sometimes it is not worthy to spend a long time to get a feasible solution[3]. The reason to cause this problem is that PSO doesn’t make full advantage of the information from the computation; instead, it only uses the information of global optimum and individual optimum in every iteration. Besides, the algorithm itself doesn’t have an optimization mechanism to eliminate the bad candidate solution so that it has a slow convergence speed [4],[5].
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Methane Fermentation Method and Methane Fermentation Device

Methane Fermentation Method and Methane Fermentation Device

[0008] Further, conventionally, the amount of the sludge from the solid-liquid separation unit returned to the methane fermentation tank and the amount of the liquid waste from the solid-liquid separation unit discharged out of the system are set constant without being particularly controlled. However, when the load (due to charging amount and concentration) of the organic waste in the methane fermentation tank increases, while the viscosity of the fermentation liquid in the methane fermentation tank increases, thus also increase the sludge concentration and the viscosity of the fermentation liquid taken out of the methane fermentation tank. The sedimentation speed of sludge in the sedimentation type solid-liquid separation unit tends to decrease as the sludge concentration and the viscosity of the fermentation liquid increase. Therefore, the sedimentation time may not be ensured sufficiently due to the fluctuation in the load of the organic waste in the methane fermentation tank, and it may be difficult to return the sludge that is enough concentrated to some required concentration to the methane fermentation tank. Further, the properties of the liquid discharged from the sedimentation type solid-liquid separation unit become worse, which makes the discharge treatment cumbersome. Therefore, in the case where methane fermentation is performed with the return amount of sludge from the sedimentation solid-liquid separation unit to the methane fermentation tank and the discharge amount of a liquid from the solid-liquid separation unit out of the system being constant, it is necessary to take into account a safety factor considering the fluctuation in the load of the organic waste in the methane fermentation tank and increase the tank capacity of the sedimentation type solid-liquid separation unit, which complicates and enlarges the apparatus configuration. [0009] Patent Publication 1: Japanese Patent Application Laid-open No. 2006-255571.
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Konsep Data Warehouse - Repository UNIKAMA

Konsep Data Warehouse - Repository UNIKAMA

Partial backup - As the name suggests, it does not create a complete backup of the database. Partial backup is very useful in large databases because they allow a strategy whereby various parts of the database are backed up in a round-robin fashion on a day-to- day basis, so that the whole database is backed up effectively once a week.

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PPSI02 Konsep Informasi

PPSI02 Konsep Informasi

Empirics Empirics Study of the Study of the physical characteristicsphysical characteristics of the medium of of the medium of communication communication Terkait dengan hasil pene[r]

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Implementasi MultiObjective Optimization On The Basis Of Ratio Analysis (MOORA) Untuk Menentukan Kualitas Buah Mangga Terbaik | Pasaribu | JURIKOM (Jurnal Riset Komputer) 1 PB

Implementasi MultiObjective Optimization On The Basis Of Ratio Analysis (MOORA) Untuk Menentukan Kualitas Buah Mangga Terbaik | Pasaribu | JURIKOM (Jurnal Riset Komputer) 1 PB

Metode Multi-Objective Optimization on the Basis of Ratio Analysis (MOORA) termasuk dalam bagian MCDM yang mampu melakukan proses sekaligus mengoptimalkan dua atau lebih Atribut (sasaran) yang bertentangan dengan batasan tertentu dari rating kinerja pada setiap alternative pada semua atribut[5]. Metode MOORA dapat membantu dalam pengambilan keputusan suatu kasus, akan tetapi perhitungan dengan menggunakan metode MOORA ini hanya yang menghasilkan nilai terbesar yang akan terpilih sebagai alternatif yang terbaik.

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comparison of ruminal fermentation characteristics and microbial

comparison of ruminal fermentation characteristics and microbial

tration of buffalo were not different with those found in cat- tle. Furthermore, the most pronounced effects were remarkably significantly higher in nutrient digestibilities of buffalo than those in cattle particularly those of DM, OM, CP, NDF and ADF of digestibility. However, the feed efficiency utilization by cattle and buffalo depends on the type of diet and of the animal ability to digest and utilize the end prod- ucts of the digestion keeping losses to a minimum. The utili- zation of poor quality feeds rich in structural carbohydrates of cell wall only is possible due the presence of microorgan- isms exiting in the rumen-reticulo and large intestine. Wide differences have been detected between cattle and buffalo in relation to the total process of digestion and utilization of various feeds. These differences include: anatomy and physi- ology of digestive system, feed intake and digestibility, rumen microbial metabolism, rumen ciliate protozoa popula- tion, etc. (Franzolin, 1994) Calabro et al. (2008) have carried out in vitro studies with rumen fluid incubated with common feedstuffs for ruminants. It was found that gas production was lower for inoculum derived from buffalo than for sam- ples from the rumen of cattle. On the other hand, Wanapat (1984) who reported that DM degradability in buffalo was higher than that in cattle, regardless of type of rice straw
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Percubaan UPSR BI Julau - Sumber Pendidikan UPSR(ANSWERS)

Percubaan UPSR BI Julau - Sumber Pendidikan UPSR(ANSWERS)

Personal Pronoun & Possessive Adjective Medium 20.. Comparison of Adjectives Medium 21.[r]

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Profil Fermentasi Rumen Sapi Pejantan Feedlot Dengan Administrasi Sikat Stimulator Mekanis Rumen (Rumenfibe ®).

Profil Fermentasi Rumen Sapi Pejantan Feedlot Dengan Administrasi Sikat Stimulator Mekanis Rumen (Rumenfibe ®).

This master study would not succeeded w ithout the firm support and prayers of my beloved parents Zakir Has and Nurbayani, and my beloved brothers Farhan, and Ises. Thanks a lot of the support from huge family of H. Ramli; Nurzakiah, Wiwit, Inel, Cecep, Desi, Sian, Neneng, and special for Asmadi that always thought me wise thinkings. I am also grateful to my classmates and others such, Ali, Ridho, Dila, Rossy, Tekad, Andre, Putri (Alm.), Nur, Mega, Teguh Wahono, Kaleem Saleem and to all of them whose names are not mentioned here. A special thanks to Devide Maric Hersade who always accompany me in any moment since studying in IPB. I am very grateful to Gita Swara Pascasarjana IPB and those members such Dr. James Unitly, F. Ochieng (Alm.), Silvia, Weni, Donal, Lista, Merry, Janet, Dedi, Desi, Iswahyudi, Ella, and many others that gave me spirit in a voice.
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Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium | Hidayat | Indonesian Journal of Biotechnology 24195 48078 1 SM

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium | Hidayat | Indonesian Journal of Biotechnology 24195 48078 1 SM

Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potential as a medium for lipase production. The objective of this research was to characterize lipase from Aspergillus niger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate speciicity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate speciicity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and v max about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.
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