A study of some effects of progesterone and pregnant mares' serum (PMS) on reproductive phenomena in the anoestrous Romney ewe : a thesis presented at Massey Agricultural College in part fulfilment of the requirements for the degree of Master of Agricultural Science in the University of New Zealand

Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author.

( I THE 0 T OU

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At 1 tdat

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MASSEY AGRICULTURAL CO!...LEGE LIBRARY PALMERSTON NORTH, N.Z.

a s y Agi ieul tur 1 Coll:... o in . r·q 1:re ent for Degr of

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MASSEY AGRICULTURAL COLLEGE LIBRARY

PALMERSTON NORTH. N.Z.

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n.

a l"st oft t o into 10 g

tb t

a ind1 at d in Tabl 2. It ill b not 2

t grou 2 c nd 3 c inj t c d ily; and

t rou

5

a eiv d 1 rr.l. of oil ( chi ) per ~ for

4 •

2. The 1nj ctio

Inj ctions eomr .cnc on Janu y2n t 9.30 ornin

}

injections were commenced thereafter between

a.oo

and 8.30 am.

and completed by 10.00 am; and evening inj.ections ere given b tween

6.45

and

7.45

^pm. (group 2 e and

3

c only).

The hormones ere injected subcutaneously in the neck region, alternately on the right and left sides.

3. Detection of oestrus

The vasectomised rams were raddl.ed with blue tupp1ng paste and the others w1 th red. Each ram as marked twice daily until January 12th and thence only once daily until January 20th, by which time all ewes had been slaughtered.

The e es were yarded and inspected t ice daily for signs of oestrus. A ewe as listed as showing positive signs of oestrus if she as clearly marked over the rump and tail region.

4.

The slaughter and collection of specimens

The five members

ot

each group which were to be

slaughtered were selected at random the day before slaughter, and kept in a pen that night. Early next morning they

were taken to the local freezing orks and ere the first sheep to go along the chain. The ewes were killed in batches of

fifteen, since this as the largest number than could be handled each day without causing too much i.nconvenicnce at the orkso As the ewes went along the chain the head and reproductive

tract of each as removed and labelled. The pituitary gland of each e e was then removed as quickly as possible and

placed in 10 ml. of acetone. All glands were in acetone within 75 minutes of killing. The ovaries were placed in

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over a period of about 8 days under the influence of

progesterone. The effect of the cell enlargement and the formation of the uniform high columnar epithelium has been to cause an increase in folding of the epithelium lining the

villi. The stromal nuclei have become rounded and definitely stain more clearly. The oedema ·Of the stroma is quite rrarked and the number of lymphocytes is greatest, as is th development of the base of the crypts at the end of this period.

The next stage probably includes secretion and storage, but there ere no sheep killed for a further 8 days. What is probably the third stage, ho ever - that of regression to the anoestrous state - is apparently seen in the ewes of group Lt. a ii. The villi had become lea ell defined and in some

cases the ^atrorr~ nuclei had become shrunken. The most striking change was that of the epithelium, hiD h~ though still fairly high, had taken on a psuedostratified appearance. The presence of occasional distended cells in the epithelium lining the villi is taken as evidence of a previous secretory phase.

4.

Vagina

I.n Table 16 are presented the data concerning the vaginas in the control groups - 5 a and 5 b. Table 17 contains the findingsin groups

4

a and

4

b, whilst Table 18 comprises the remainder. It is intended that a full description be given of the picture as represented by the control e es and that this will serve as a basis for comparisOn bet een all groups.

a. Detailed study of the results in Table 16

The most strikin feature in the anoestrous ^e~e is the

variability bet een rr.ost sheep, in the majority of characteristics examined. There was also noticed a considerable lack of

1 ics -

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1 115 H

121 H

150 H 329.49

11 l AG 9 ^H 55 H

256.49

4

b 1 19 .H

122 H 110.49 168.49 280,49

4 b 11 3 AG 48 H 56.47 229.49 385.49

-68-

TABLE

l l

______

^_,..

Vaginal characteristics

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14 ⁰ AOS

+

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++ DDSS D-I ++ DSS D-I

+++ DDSS D ++ DS D-I ++ DS D

++ DDSS D

+++ DDSS D-I +++ SS I +++ DDSS D-I

++ DDSS D-I +++ DSS D-I

++ DSS D-I +++ DSS D-I ++ DDSS D-I ++ DDSS D-I

++ DS D-l-_~ I

~m CX)Q) rlr-t ..-1

::s ^{~ r-t} v

r-t 0 Q) r-t . r-1 _(I)

t:.

^C1l _Q) ⁽⁾ _::s

0 0 I:Q~

GF 0 GF 0 GF

o-

GF

o-

G 0+

GF O DG 0 G 0

GF +

F

o-

GF 0 GF 0- GF 0

GF 0+

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G 0+

GF ⁰⁺

GP 0+

- group 4

0 ~ oM

+=>

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oM

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CXl () oM ~ oM ~

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0 0

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+ 0

Height of Epithelium

bO

Q)

Jt.t

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c:r:~ ~

30 55 80 60

'50 50 55 50

50 50 80 120 80

60 50 60 5 45

(I) (t) ~

~t::::

20-35 35-70 35-120 35-140

20-80 35-80 20-75 30-80

35-95 35-60 45-120 85-140 35-120

35-95 35-60 20-95 70-140

20-50

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..

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++ D + + 0

++

s

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++

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^{I ++ 0}

++ D +

++ + 0

3 c 1 ++ + + 0

++ D + + 0

++ D-I 0 ++ 0

++ D + 0

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1 ++ ^I 0 ++ 0

++

s

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(

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11.

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Accor in the qui

on lin 1 s t1 f tory for qu ti tiv. a It 1 llno r ov r autfielent r , tho v r1 ·ea

it 1 rel t ve~ teep 1n rel t1 t th

vari

•

to corr ct ror b - eight !f:C nc • Th1 :ituat on al old

for the r sul of t e n ay f th pituit y gl for go o phin •

6. ^{ults of} snaya of the 1tu1t go dot

gl a for

Th 100 p.itu1 y lan of e e ro

2, .3, l~ d 5 re a y 1n five eeri ry of

t r oult Al o 11 t 1n

table r th ro lt o t o le el {15 iu 30 1u ¹ for ch eerie a

cont 1 chicks.

l l th re to of the

len of ou s2 a 3 ( S rie 1)

a~oG.ff'-"1 u ing th I/' thod. It can be

.

^~ n that do level ( 15 /ch c ~9

to give

sult

hieh e

lo e ounte to helf th chick

t1 factory.

d the t et r

1ght of the rem It 111

r did not itfer tro the cont ol • o serv th t 1n Seri 2 t ere no 1f't' renee etwcen the four sub-group • In

s

^r 3

·ere

follo ng onoly is of v 1nd1ct t t the l.o er v rage 1n 3 c 1 i t signi cantly diffe t 3 c 11,

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J. Counc. Sci. en~

s., .... Aust. 12:18.

(1943). Aust. Counc. Sci.

Res.... Bul... ~6.

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