ACKNOWLEDGEMENT
The reverence is to the omnipotent “All mighty Allah”
who enabled the author to complete this study successfully.
The author wishes to express his gratitude to his supervisor Professor DR. A.K.M. Saifuddin, Director of clinic:
for his suggestions, guidance, encouragement, and cordial support throughout the period of study.
The author expresses his deepest sense of gratitude and profound regard to Prof. Dr. N. C. Debnath, VC, Chittagong Veterinary and Animal Sciences University.
The author avails himself of the opportunity of expressing his sense of appreciation and sincere gratitude to Prof. Dr. Matin Pradhan, Dean, Faculty of Veterinary Medicine.
The author desires to express his profound appreciation, gratefulness and heartfelt thanks to Ashraf Ali Biswash, Director of External Affairs, Chittagong Veterinary and Animal Sciences University.
The author is very much grateful to Dr. s. K. m. Azizul islom'' Assistant professor, Deportment of physiology, pharmacology and Biochemistry for his constant inspiration, suggestion and extending helping hand to perform this research also for providing me all type of facilities in Biochemistry laboratory.
The author is deeply thankful to DR. A. S. M. Golum Kibria, Lecturer Department of Anatomy and Histology for his kind suggestion and cordial help through out the laboratory work and also help in report writing.
The author expresses his gratefulness to Dr. shormin choudhury, Assistont Professor, Department of pathology and porasitofogy, chittogong veterinary and Animol sciences university., Bangladesh, for assisting the author with statistical analysis of data and his valuable advices to write report.
The author would like to give thanks to the staffs, employees, laboratory technicians and the personnel of Laboratory working in the Department of Physiology' Pharmacology and Biochemistry, Chittagong veterinary and Animal Sciences University (CVASU) for their assistance in chemical analysis of samples.
The author expresses his boundless gratitude and deepest appreciation to his parents for their encouragement and well wishes through out the academic life.
The author also expresses his gratefulness to all of his friends for their kind co-operation and encouragement to complete the study.
The author
CONTENTS
Chapter Title Page no.
Acknowledgement iii-iv
Abbreviations vi
Abstract vii
1 Introduction & Objectives 01-03
2. Review of literatures 04-10
3. Materials and Method 11-16
5. Results 17-19
6. Discussion 20-22
7. Recommendation 23
8. Conclusion 24-25
9. References 26-28
10 Annex 29
-:
List of the Tables
:- SerialNo.
Title Page No.
01 TLC-result of antibiotic residues (Ciprofloxacin) found in liver Samples
17
02 Analysis of TLC-result of antibiotic residue (Ciprofloxacin) found in broiler liver:
17
-:
List of the Figure
:-Serial
No. Title Page No..
01 TLC result of antibiotic residue in broiler liver 18
-: List of the Plates:- Serial
No. Title Page No.
01 Collected liver sample in Petri dishes 19
02 Filtration of liver extract (LE). 19
03 Separation of liver extract from fatty portion 19 04 Prepared liver extract in Eppendorf tube 19 05 Pointing on TLC paper by liver extract 19
06 Detection of AR in LE under UV light 19
LIST OF ABBREVIATIONS
Abbreviation Elaboration
AGP Antibiotic Growth Promoter
BBS Bangladesh Bureau of Statistics
DNA Deoxiriboneuclic Acid
DLS Directorate of Livestock Services
DW Distilled Water
EU European Union
et al.
AssociatesFAO Food and Agriculture Organization
FPT Four Plate Test
GC Gas Chromatography
GDP Gross Domestic product
HPLC High performance Liquid Chromatography
MRL Maximum Residual Level
NOAH National Office of Animal Health
TLC Thin Layer Chromatography
UV Ultra Violate
USA United States of America
WHO World Health Organization
ABSTRACT
Nowadays broiler may treat most important source of animal protein for the large number of people of Bangladesh. Day by day poultry industry develops gradually and the use of antibiotics also increases not only as growth promoter as well as for controlling infectious diseases. But indiscriminate use of these drugs deposits some residue in different body tissues of poultry. Antibiotic residue in poultry tissues is an important issue which needs accurate detection, proper monitoring and sufficient legislation to ensure food safety of people. This research
highlights the importance and existence of antibiotics residue in meat. My present study was conducted for the detection of existence of antibiotic residue in broiler liver.
Here I use the TLC for the detection of residual antibiotic in broiler liver as it is easy to perform, allow simultaneous analysis of large numbers of samples and give rapid and accurate results in comparison to other methods. In this survey liver extract prepared from 2gm of liver by using trichloroacetic acid 30% and diethyl ether. After pointing and running on TLC plate, chromatograph observed under UV light. Among the 10 samples from various shops 6 positive samples were found, so about 60% of samples contain antimicrobial residue with the standard error of
0.090341. It was stated that probably in some cases chicken meat producers do not respect regulations about withdrawal periods of the veterinary products.
The author
Key words: Antibiotic, residues, public health hazard, maximum residual limits.
