PREVALENCE OF NEWCASTLE DISEASE IN WINTER SEASON IN CHITTAGONG AND EFFICACY OF CHALLENGE VACCINE AGAINST
NEWCASTLE DISEASE
A Report Submitted by:
Intern ID: A-08 Roll No. : 2006/09 Reg. No. : 247
Session: 2005-2006
AS THE PARTIAL FULFILLMENT FOR THE DEGREE OF
DOCTOR OF VETERINARY MEDICINE (DVM)
Faculty of Veterinary Medicine,
Chittagong Veterinary and Animal Sciences University,
Khulshi, Chittagong - 4202.
May, 2012.
PREVALENCE OF NEWCASTLE DISEASE IN WINTER SEASON IN CHITTAGONG AND EFFICACY OF CHALLENGE VACCINE AGAINST NEWCASTLE DISEASE
A Report Submitted as Per Approved Style and Content
Signature of Author Name: Md. Saiful Bari
Signature of Supervisor Name: Dr. Md. Masuduzzaman
Intern ID: A-08 Roll No. : 2006/09 Reg. No. : 247 Session: 2005-2006
Designation: Professor Depatment of
Pathology and Parasitology.
Chittagong Veterinary and Animal Sciences University
Khulshi, Chittagong - 4202.
Chittagong Veterinary and Animal Sciences University Khulshi, Chittagong - 4202.
May, 2012.
CONTENTS
Chapt
er Topics Page
No.
Acknowledgement i
Acronyms used ii
Abstract iii
I Introduction 1-2
II Review of literature 03-10
III Materials and methods 11-17 IV Result and discussion 18-22
V Conclusion 24
VI References 25-28
VII Appendix 29
-: List of the Tables:-
Table
no. Title Page
No.
4.1 Diagnosis of the diseases on the basis of
postmortem examination 19
4.2 Comparison of mortality rate before and after
challenge vaccination in ND affected flock 20 4.3
Results of HI titre of Newcastle disease in Broiler (Infected flock; Before challenge vaccination)
21
4.4 Results of HI titre of Newcastle disease in Layer
(Infected flock; Before challenge vaccination) 22 4.5 Results of HI titre of Newcastle disease in
broiler (After challenge vaccination) 22 4.6 Results of HI titre of Newcastle disease in layer
(After challenge vaccination) 23
-: List of the Figures:-
Figur
e no. Title Page
No.
3.1 Photograph of Postmortem Findings of
Newcastle disease. 17
3.2 Photograph of HI Test procedure. 17
-: List of the Graphs:-
h No.Grap Title Pag
e No.
4.1 Comparison on HI titre level in broiler (before and
after vaccination) 23
4.2 Comparison on HI titre level in layer (before and
after vaccination) 24
ACKNOWLEDGEMENT
All praises are due to Almighty “Allah” who has created everything of the nature and who enable the author to complete this report. The author does not have adequate words to express his heartfelt sense of gratification sincere appreciation to his benevolent teacher and report supervisor. The author expresses his sincere gratitude, heartful respect and immense indebtness to his supervisor Dr. Md. Masuduzzaman, Professor. Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University for his valuable advice, guidance, suggestions, inspiration and who was involved with this study through its inception. Special thanks to Ashraf Ali Biswas, Professor & Director (External affairs), Department of Animal science and Animal nutrition, for his valuable advice and co-operation. I would like to give special thanks to DR. Md. Mahmudul Hasan, Assistant professor. and DR. Md.
Shahneaz Ali khan, Assistant professor. Department of Physiology,Biochemistry and Pharmacology, Chittagong Veterinary and Animal Sciences University for his valuable advice about scientific report writing. FinallyI want to give special thank to DR. Md. Zohorul Islam, Lecturer. Dept. of Microbiology, Chittagong Veterinary and Animal Sciences University for giving valuable advice about report writing and for providing opportunity to test the collected sample. I would like to express my deep sense of gratitude and thanks to Vice Chancellor, Dr. A S Mahfuzul Bari and Dr.
Md. Masuduzzaman, Dean, Faculty of veterinary medicine, Chittagong Veterinary and Animal Sciences University.
Supervisors’ help and co-operation have been received from many persons during the tenure of this place of report. The author is immensely grateful to all of them, although it is not possible to mention every one by name.
The Author:
i
ACRONYMS USED
ND Newcastle Disease BBS Bangladesh Bureau of Statistics
BLRI Bangladesh Livestock Research Institute
CDC Center for Disease Control GDP Gross Domestic Product HA Haemagglutination
HI Haemagglutination Inhibition NA Neuraminidase
OIE Office International des Epizooties’
SPSS Statistical Package for Social Sciences
ii
PREVALENCE OF NEWCASTLE DISEASE IN WINTER SEASON IN CHITTAGONG AND
EFFICACY OF CHALLENGE VACCINE AGAINST NEWCASTLE DISEASE
Abstract
A study was undertaken to determine the prevalence of Newcastle disease in poultry farms of Chittagong region during the month of January and February, 2012. In this period the efficacy of challenge vaccine against Newcastle disease was determined.
Disease diagnosis was conducted on the basis of history, clinical findings and post- mortem lesions. And challenge vaccine efficacy was determined on the basis of laboratory test (HI test before and after vaccination) and field trials. Birds taken from 52 broiler farms and 15 layer farms were examined during the study (mortality before and after vaccination). Prevalence of Newcastle disease was (8.96%). Due to vaccination with ND killed, (Lasota) at initial stage of infection the mortality rate reduces to zero and HI titre of the broiler farms increases as follows 5.4 to 7.55, 4.6 to 7.25, 4.65 to 7.70 and in the layer farms as follows 4.56 to 7.13, 5.0 to 7.81, 4.93 to 8.13. The HI titre level were insignificant in difference (P>0.05) among the farms
in case of both before and after vaccination. For HI test 60 blood samples (20 from each of 3 broiler farms) and 48 eggs (16 from each of 3 layer farms) were collected and tests were performed.
Key words: Clinical, Necropsy findings, HI test, Mortality, Vaccination.
iii
