Analisis QTL sifat ketahanan penyakit BLAS (Pyricularia grisae, Sacc) pada populasi hasil persilangan IR64 dengan spesies padi liar Oryza rufipogon, Griff.

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1 ABSTRACT

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Utami. QUANIT~ATIYE Trait Loci (QTL) Analysis of Bhst

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(Pydcdda g r k a , Sam) Resistance in Popuhtian Derived from Crossing B a s g o

g2su22= between IR64 and Wild Rice Species, Orym -on, Griti) mder the direction

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Qcn.lrt Q of HAJRZAL ASWIDINNOOR, ED1 GUHARDJA, ASEP SETIAWAN, and

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3 S $ @ ' ! ? . SUGIONO MOEWOPAWIRO.

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Wild spcies of rice are the one of unique sources of genetic variation, which ~ " s n _{P, I}

can

_{be used for genehc improvement especially for quantitative traits. Blast disease}

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high

percentage of yield losses on

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rice pduction. The improvement of blast resistance

in

rice is difficult due to the 0-0 X T C s

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3.

complexity of the idmi- of this trait as well as the dynamic of the pathogen.

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f 9 g g Wild s p i e s of Orym can be exploited to emich the blast mistance controlled by

~ E ~f Squantitative trait loci (QTL) in the existing wieties. Oyza &pgon, Griff (AA

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genome; Acc No. IRGC#105491), om of the wild rice species,

has

ban

indicating to

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contab QTL of blast in

BC&

population of cross with

IR64.

Marker assisted 3

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selection (MAS), therefore, could

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osd

to s p e d up the -cry of recurrent parent

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g e t o o r n e i a ~ ~ ~ T h e ~ ~ e r a l l o b j e c t i v e o f t h i s ~ h w a s t o ~ y z e

rr

=

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a

tbe blast QTL wighated

froIll

interspeslfic muss between IR64 and wild rice spies,

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v. C Q

z:

z.9 2. 0 . d nbased on genetic, htrogression and mapping a d y s ~ s . This study was

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exp&d to

&st

the M n g program for

tbe

development of new blast mistant

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7.~3 ricelines.

$ i% o o _This study consisted of 3 experiments. First: Analysis of blast resistance

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inhexi-

to dctcd

genes action wming

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0. nJflpgon oontribvhng to

the

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resisEaace

of

three

blast t ~ l ~ e ~ . Two pamb (PI: R 64 and

Pa:

0. M p g o n ) ,

F1, Fz,

X = 3

F W B(=Pt and BCfi ~ o nwen s s c a d on the basis

of

disease leaf ares (O/&M)

s

=I 8 s rwulting h m

tht

three

bIast

races inoculation in gmxbuse.

Tht

genetic amdmof

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this study consisted: m a n peration analysis, ckcmhtiofl of genetic models and

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.

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the

Ivariance compmmt and heritability.

Tbe

d i n g t& evaluated tbe

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g o a k w of fit

of

the three parameters m h l (mid pmb [ml additive [dl

and

B dominance [h] effect). When the

~~

_{madel did not show a good fit, a}

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E six-pramdm joint scaling testwas usedto d e b m h the adequaq of a digmic

[A epistatic m o d , which provide

eshates

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of the three qmtatic pameten: additive x

0 additive [iJ, additive x dominance tj] and dominance x s

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9 wild rice s p i e s 0. ngipogon- T'he MAS technique was used to detect the E

>

O.n&gon in-on

into

IR64 in X 2 F 3 population. Third: QTL analysis for

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blast resistance on selected lines (fmm Exp. 2). Twenty selected lines were multiplied

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s 0 iadividualIy to generate a population and used for blast QTL mapping based on

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statktical approach of s u e point Bnalpk (SPA), interval mrlpping

(IM)

and

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composite interval mapping (CIM).