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Isolation and characterization of polymorphic microsatellite markers in Amorphophallus paeoniifolius (Dennst.) Nicolson, Araceae

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http://www3.interscience.wiley.com/journal/118523830/abstract?CRETRY=1&SRETRY=0

Isolation and characterization of polymorphic microsatellite

markers in Amorphophallus paeoniifolius (Dennst.)

Nicolson, Araceae

E. Santosa*§, C. L. Lian‡, Y. Pisooksantivatana† And N. Sugiyama*

*Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1 Bunkyoku, Tokyo 113–8657, Japan, †Faculty of Agriculture, Kasetsart University, Bangkok, Thailand, ‡Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho

1-1-8 Nishitokyoshi, Tokyo 188-0002, Japan

Correspondence: Chunlan Lian, Fax: +81-424655616; E-mail: [email protected]

§Present address: Faculty of Agriculture, Bogor Agricultural University, Kampus IPB Darmaga Bogor 16680, Indonesia.

Copyright © 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd

Abstract

Amorphophallus paeoniifolius is an herbaceous perennial tuber crop distributed widely in many Asian countries. We isolated 19 polymorphic loci from A. paeoniifolius using a dual-suppression-PCR technique. These loci provide microsatellite markers with high polymorphism ranging from three to 24 alleles per locus. The observed and expected heterozygosities ranged from 0.521 to 0.854 and from 0.766 to 0.930, respectively. This high allelic diversity indicates that our markers are suitable for a population study in A. paeoniifolius.

Keywords

Araceae • elephant foot yam • microsatellites • tuber crop

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