Cloning of cryIA Fragment Encoding Toxin Domain Using pGEM-T Vector
Bebas
6
0
0
Teks penuh
Dokumen terkait
[r]
Bagian ujung 5’ dan tengah gen MmPMA telah diisolasi dengan PCR menggunakan cDNA total sebagai cetakan dan primer pma yang didesain dari beberapa tanaman, sedangkan bagian ujung
Results Molecular engineering was applied to create a fusion gene construct scEDIII-PIGS consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue
marneffei โดยวิธี Northern blot analysis พบการสะสมของ RNA transcript ใน conidia ซึ่งเปนสภาวะสงบของเชื้อ แตในขณะ ที่เชื้อมีการเจริญเปนสายรา ที่ 25 องศาเซลเซียสจะพบการลดลงของ RNA
The gene encoding the native GI-MCP failed to express under any of the culture conditions, however, the modified synthetic gene encoding the GI-MCP expressed the recombinant iridovirus
To isolate the cDNA sequence, primers were designed according to the sequence data obtained and used in a two-step RT–PCR reaction as fol- lows: First-strand cDNA synthesis was
Tạo cấu trúc chuyển gen và A.tumefaciens tái tổ hợp mang gen codA Các vector biểu hiện gen thực vật pBTII mang promoter 35S, rd29A và HSP tương ứng để điều khiển biểu hiện gen codA ở
