Arcangelisia Flava Increases Rats' Leukocytes but has Biphasic Effect on Rats' Lymphocyte

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The

l"t

lnternational

conference

on

Pharmaceutics

&

Pharmaceutical

Sciences

Proceedings

ISBN : 97

8-5A2-72333-0-0

(Letter of National ISBN Agency No' 4L27lE'slp/03'2015 Date 18 March 2015)

1st edition Proceeding

Published bY:

Faculty of Pharmacy Universitas Airlangga

Surabaya, lndonesia

Address:

Kampus B Jl. Dharmawangsa Dalam

Surabaya 60286

Phone +62315033710

Fax +62 31 5020514

Website : www.icpps2014.com or wwwff'u na i r'ac'id

Email: icppinfo@gmail.com

rsBN

1?A-t0e-?e333-0-tl

(3)

PREFACE

From Chairman

It

is

our

pleasure

to

present

you

the

proceedings

of

The Lst lnternational

Conference on

Pharmaceutics

and

Pharmaceutical Sciences (ICPPS) organized

by The

Faculty

of

Pharmacy

Universitas Airlangga Surabaya lndonesia.

The proceeding was produced based

on

papers and posters presented at The

1st

lnternational

Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS), held in Surabaya, lndonesia,

14-15November2014.

The proceeding clearly reflects broad interest, from the participants that coming from all around the world.

The papeJ$presented were pharmaceutics and biopharmaceutics; requirements on howto evaluate

molecufp!

in

discovery

and their

appropriateness

for

selection

as potential

candidate;

their

development

in

context

of

challenges

and

benefits,

together

with

associated

time and

cost

implications and also requirements to progress through pre-clinical and clinical.

ln this an opportunity, lwould like to express my appreciation to the editorialteam of the proceeding

who have been working hard to review manuscripts, and making the first edition of this proceeding

be possible.

I would

like also

to

thanks

to

all

invited speakers and presenters

who

participated

in

The 1st

lnternational

Conference

on

Pharmaceutics

and

Pharmaceutical Sciences (ICPPS)

and

your

contribution to this proceeding.

Finally,

I

hope

this

proceeding

will

_{give contribution}

to

the

Pharmaceutics and Pharmaceutical

Sciences research.

Chairman,

(4)

COMMITTEE

Advisor

Chairman

Editor

n

,rtn

Committee:

: Dean

of

Faculty

of

Pharmacy Universitas Airlangga

:

Dra.

Esti

Hendradi,

M'Si"

Ph'D' APt'

: Dr.

Wouter

U. Hinrichs (Netherlands)

Prof. Kozo TakaYama (JaPan)

Dr.

Srinivas

Mutalik

(lndia)

or.

ttuttakorn

Baisaeng

{Thailand)

Prof.

Dr.

WidiiSoeratri'

DEA

(lndonesia)

Prof.

Dr.

Widji

Soeratri,

DEA'

APt'

Prof. Dr.

Siswandono, MS'' APt'

Prof

.Dr.

Tutuk Budiati' MS'' APt'

Prof. Dr.

rer'nat'

Muhammad Yuwono' MS''

Apt'

Drs.

Hadi Poerwono, MSc''Ph'D'

APt'

Drs.

Marcellino Rudyanto' MSi''

Ph'D'

Apt'

Drs.

Bambang Widjaja, M'Si' APt'

Drs.

SugiYartono,

MS,

APt'

Dra.Tutiek Purwanti, MSi' APt'

Dra.Tristiana Erawati, MSi' APt'

Dra.Retno

Sari, MSc,

APt'

Dra.Dewi lsadiartuti, MSi' APt'

Dra.Noorma

Rosita,

MSi' APt'

Dr.

DwiSetyawan,

SSi,

MSi" APt'

Or.

ni"tt.

Primaharinastiti'

SSi'

MSi'

Apt'

Dewi

MelaniHariyadi'

SSi'

M'Phil''

Ph'D'

Apt'

HelmY Yusuf, SSi,

MSc'' Ph'D' APt'

Dr.

rer. nat. wtaria

l-ucia

Rrdhani Dwi Lestari' SS|'MPharmSci''

Apt-tvt"lanny lka Sulistyowati' SFarm'

M'Sc'

Apt'

Febri

Annuryanti, SFarm'M'Sc'

Apt'

-

'

M.

Faris

Adrianto, MFarm' APt'

(5)

TABLE

ofCONTENT

Preface

from

Chairman

Committee

...

ii

Table

of

Contents

....--..--..--.

...

iii

Author

lndex

...

iii

AUTHQR INDEX

COMPARISON OF SODIUM STARCH GLYCOLATE AND CROSSCARMELLOSE SODIUM AS

SUPERDISINTEGRANT IN MEFENAMIC ACID FAST DISINTEGRATING TABLET

Adeltrudis Adelsa D, Oktavia Eka Puspita, Amalia Ayuningtyas, Marulita lsadora ,...,... 1

STUDY EXPRESSION OF HUMAN ERYTHROPOIETIN EXPRESSSION IN MAMMALIAN CELL

Adi Santoso, Popi Hadiwisnuwardhani, Yana Rubiana, Yulaika Romadhani, Endah Puji

Septisetyani, Dyaningtyas D.P. Putri

ANTIOXIDANT STABILITY ASSAY OF ALPHA TOCOPHERYL ACETATE IN SOL]D LIPlD NANOPARTICLE

SYSTEM (LlPlD BASE BEESWAX AND MONOSTEARIC GLISERYL 50:50) Anggie Widhi, Noorma Rosita, Widji Soeratri ...

A BIOACTIVE BOVINE HYDROXYAPATITE4ELATIN IMPLANT FOR IN VITRO GENTAMIC]N RELEASE

Aniek Setiya Budiatin, M. Zainuddin, Junaidi Khotib, Diah Himawati ... 13

EFFECT OF COMPARISON SURFACTANTAND COSURFACTANT IN WATER/OIL MICROEMULSION IN

RELEASE OF OVALBUMIN Microemulsion

Water/Oilwith

Surfactant (Span 8O-Tween 80) :

C-osurfactant (Ethanol)

=5:L,6:!,

and 7:1)

Anisa Rizki Amalia, Riesta Primaharinastiti, Esti Hendradi

...,...,

... 18

ANALYSIS OF MYCOLIC ACIDS CLEAVAGE PRODUCT OF Mycobacterium tuberculosis BY GAS

CHROMATOGRAPHY-FLAM E IONIZATION DETECTOR

Asri Darmawati, Deby Kusumaningrum, lsnaeni, Muhamad

Zainuddin

... 21

PERIPLASMIC EXPRESSION OF GENE ENCODING ANTI-EGFRvIIISINGLE-CHAIN VARIABLE FRAGMENT

ANTIBODY USING PelB LEADER SEQUENCE lN ESCHER/CHIA COLI

lGrtika Sari Dewi, Debbie Sofie Retnoningrum, Catur Riani, Asrul Muhamad Fuad ...24

IN VIVO ANTIMALARIAL ACTIVIry OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION OF

Alectryon serrotus LEAVES ON Plasmodium bergheilNFECTED MICE

Aty Widyawaruyanti, Uswatun Khasanah, Lidya Tumewu, Hilkatul llmi, Achmad Fuad Hafid,

Indah S Tantular

...

... 30

PROFILE OF COMMUNIry PHARMACISTS KNOWLEDGE IN PATIENT ASSESSMENT WITH INFLUENZA

SYMPTOMS AND ITS PRODUCTS

(6)

&

SOLUBILIry AND D]S5OLUTION STUDY OF KETOPROFEN

-

HIDROXYPROPYL'P-CYCLODEXTRIN

INCLUSION COMPLEX (Prepared by Kneading Method)

BambangWidiaia, Achmad Radiaram, Arafah Zulhana

"""""':""""

"""""""""

37

FoRMUIATIoNANDSTABILITYTESTINGoFMELoXICAMSoLIDDISPERSIoNGEL

BudipratiwiWisudyaningsih,lnkaDewiNurAnggaraini,FersiyaWardani...'..40

EFFECT OF MENTHOLAS PENETRATION ENHANCER TO DICLOFENAC SODIUM MEMBRANE-TYPED

TRANSDERMAL PATCH

CHARACTERIZATION

^a Destria lndah Sari, Esti Hendradi, Junaidi

Khotib

""""

rJ

PHYSICALCHARACTERISTICSANDRELEASESTUDYoFoVALBUMINFRoMALGINATEMICRoSPHERES

PREPARED BY DIFFERENT coNcENTRATIoN oF ALGINATE AND BaCl, UsING AERoSoLlzATloN

TECHNIQUE

-!-:r:- .--^.r.-L.,..*inah^c

---..-... 40

Dewi Melani Hariyadi, Tristiana Erawati, sisilia Ermawahyuningtyas

MUCOADHESIVE TABLET OF ETHANOLIC EXTRACT OF SAMBILOTO (Andrographis paniculata)

AS ANTIDIABETIC USTNG CHITOSAN

DhadhangWahyuKurniawan,HeningPratiwi,andlingga|kaditya...50

PHYSICALINTERAqtIoNsTUDYoFIBUPRoFEN-STEARICAcIDBINARYMIXTURE

Diaieng Putri Paramita, Dwi Setyawan, Dewi lsadiartuti

"""""""'

""""""""""'

59

.

MOLECULAR MODELTNG AND SYNTHES]S OF L-(3,4-Dichlorobenzoyl)-l',3-dimethylurea

' ;;"';;;

r""-t-"riur*"

Siswandono, BambangTri

Purwanto

""""""""""""

53

EXPRESSIoNoFRECoMBINANTHUMANGRANULocYTE-coLoNYSTIMULATINGFAcToRWITHIN

PERIPLASMIC COMPARTMENT OF FSChETiChiO COIi USING PEIB LEADER PEPTIDE

Dian Fitria Agustiyanti, Asrul Muhamad Fuad

EVALUATION OF ANTIHYPERURICEMIC ACTIVITY FROM BULBS OF BAWANG TIWAT (EICUIhET|NC

patmifolia (1.) Merr.) BY lN VITRO AND lN VIVO STUDIES

Dian Ratih Laksmitawati, Rininta Firdaus, Yulinda, Mediana Astika

""""'

"""""72

ANTIOXIDANT ACTIVITY OF Xil%ETHANOL EKTRACT OF COMBINATION OF STRAWBERRY FRUIT

(Fragoria

x

anan(rssa Duch.) AND STARFRU 11 (Averrhaa carambola L') uslNG ABTS FREE RADICAL

SCAVENGING

METHOD

-?

Diana Serlahwaty, lndira Natalia

Timang

"""""""""'

'o

ENHANCEMENT OF SOLUBILITY AND DISSOLUTION ATORVASTATIN BY MICROCRYSTALLIZATION

METHOD

Dolih Gozali, Yoga Windu Wardhana, Ronny

Tandela

""""""""""'

rz

INVITR}ANTIMALARIALACTIVITYoFDIcHLoRoMETHANESUB.FRACTIoNoFEuco/yptusglobulus

L. Stem AGAINST Plasmodium folciporum

Elis Suwarni, Achmad Fuad Hafid, Aty

Widyawaruyanti

""""""""'

85

ATcangelisiaflovaINCREASESRATS.LEUKoCYTESBUTHASBIPHASICEFFECToNRATS'LYMPHoCYTE

(7)

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Medamrc-32'

Fdu-aqr Reorn: tK6 epCI1

Conierence

& Pharmaceutical Sciences

Arcangelisia

flava

INCREASES RATS, LEUKOCYTES

BUT HAS BIPHASIC

EFFECT

ON

RATS' LYMPHOCYTE

frsari.

Faculty of Pharmacy University of Jember; Jl. Kalimantan 37 Jember, _{lndonesia, e.puspitasari@}

Frf l'.tmavah Ulfa, Faculty of Pharmacy University of Jember,

il.

Kalimantan 37 jember, lndonesia;_@

ulillrr c: Pharmacy University of Jember, Jl. Kalimantan 37 Jember, _{lndonesia; Mohammad Sulthon Habibi.}

4ar,nary University of Jember, Jl. Kalimantan 37 Jember, lndonesia.

@

rcPtfuol4

rich

of

natural

resources,

espe-untl

rrdicinal

plant,

lndonesia has _many

to

develop medicinal

sources

fiom

plants. One

of the

potential

:'lant

used

for

cancer

chemopre-agpnt is Arcangelisia flava. This plant

to

have antioxidant and cytotoxic

aganst

breast cancer

cell line,

MCF-=nr[ity

were

considered

to

be

corre-:D

the

alkaloid content,

especially

Keawpradub

et al.,

2005). As

this

E

=ry

to

be

found in Meru Betiri

Na-;itr.rated in Jember (Koran Jakarta,

re

are eager

to

explore

further

about

mreloped as medicine,

we

have to

;afety. The sub-chronic

toxicity

assay

to

study

the

safety

of

ethanolic

nf 4- flava leaves (EEAfL) use. This

re-,mms Cone _{to determine the}EEAfL effect

,les

dnd lymphocytes

cell count

of

sub chronic EEAfL.

;ll

was done

to

determine the

EE-:'r

rmmune response, especially on

;rd

lymphocytes cell count

of

rats

,;l

chronic EEAfL.

AND METHODS

.=1s _and_extraction

frmr: ieaves were collected

from

Meru

M::nal

Park, Jember, lndonesia. They

le::ed

for

their

freshness,

old

age,

thoroughly

with

water,

then, were air

dried

followed

by oven

drying at

50 0C" The dried

leaves _{were grounded and}_sieved._The

etha-nolic extract were prepared

using

500

g

of

leaves

powder according

to

the

with

a slight modification (Keawpradub

et

al.,

2005).

The ground-dried

leaves was

sequentially extracted

with

n-hexane,

chloro-form, and ethanol. The extraction was

repeat-ed

three

times

for

each solvent. The ethanol

extract

was evaporated

under

reduced pres-sure _{(Heidolph, Laborota) resulting}_EEAfL.

EE-AfL was then suspensed in CMC Na 1% before

being _{administered to the animal.}

Animals

Male Wistar

rats (weighing 100-150

g)

were

housed

at a

constant

temperature and

light-dark cycle. Rats were

fed with

standard feed

and

water

as

libitum.

The rats

were

acclima-tized and quarantined

for

at

least 10

days

prior

to the

experiment. The animal handling

protocols

of

this

study were

in

accordance

with

the guidelines of

the

animal care

of

Uni-versity of Jember.

Experimental design

Fifteen rats

were

devided

into three

groups.

Group

I

as control,

received CMC

Na

1

%.

Group

ll

received EEAfL 500 mC/kC BW Group

lll

received EEAfL 1,500

mglkg

BW The

treat-ment was done orally

for

11 days. At

the

12th

day,

the

blood sample was collected and

ana-lyzed

further

for

leukocytes and lymphocytes

cell count.

Statistical analysis

All

data

were

presented as mean +

the

stan-I

n

(8)

@rcpfuor4

nrelofrternational

koceeding

on Pharmaceutics _&pharmar

dard error of the

mean _{(SEM). Kruskal Wallis}

assay

followed by Mann Whitney test

were

used

to

know the

significance

difference

_{be_}

tween

groups, since

the

data could

not

meet

the

_{requirement for}_{Anova analysis (p}_< 0,05).

RESULTS AND DISCUSSION

Leukocytes cell count

The leukocytes cell count _{(Figure 1)}

of

_rats_{re_}

ceiving EEAfL were increased _{significantly than}

that ofthe

control group,

either

_at_the_dose

of

500

or

1,500

mglkg

BW

Lymphocytes cell count

The

lymphocytes

cell count

of

rats

_receiving

500 mg/kg BW

EEAfL

was

increasing signifi_

cantly,

but the

_rats

treated with

_EEAiL

ai

tn"

dose

of

1,500

mg/kg

BW had

U".r""r"J

tyr_

phocytes (Figure _2).

Discussion

EEAfL seems

to

increase

the

of

normal

rats, as

it

was

cytes cell

count.

But

it

had

lymphocytes, _as

the

_higher

the

_{lymphocytes cell count.}_We

high dose

of

EEAfL decreased

system

as

it

_decreased

the

count.

the

berberine content. Berberire

rmmunosuprressive

agent.

lt

tivation and proliferation

of

_T

cytotoxic effect known

(Xu

et aL

I

AfL might

increase

the

non-spesft

system.

Leukocytes

consist of

lymphocytes,

_monocytes,

basophyls. Lymphocytes plays

rob

rmmune response,

while

_others

d

specific immune response.

Leukocytes

cells count

that

_was

while

the

lymphocytes _cell_count

creasing

after

_treated

with

_high suggested

that the other

_kinds

d

involving

in

non-specific immurp

were _{higher. Water}_extract_of_A.

EEAfL

contained berberine

_(pusp

Ulfa, 2013).

The

higher

the

dose,

macrophage

activity

_(Florentina,

EEAfL might also increase

the

_m;

rofag secara ln

Vitro

pada Tikus

rgrorp I

[image:8.535.12.535.15.735.2]

f6roup I qgrsup _ilt

Figure

1.

Leukocytes

cell count

of

rats.

_Data

represented _as_mean_{+ SEM (n =}

5). Different letter n otatio _{n exp ressed}_s_ign_{ifi ca}

ni

d iff" r"

n."

according

to

_{Mann Whitney test}_(p

< 0,05).

agrolp I

ilgralp tr

Figure 2.

LymphocyJ*,,

_count

of

_rats._Data

represented as mean + SEM (n ₌_{5). Different}

letter n otatio _nexp ressed, ign in.a

ni

O

iff*r"

*u

according

to

_{Mann Whitney}

test

_(p

< 0,05).

-2(m

g 1ym

Er(}m

- 5m0

0

2mm

1Sm

:. 1S0O 5 1SS

: lDS

g 1lxf,F 48ffi € oinc

E

i 400

lffu

o

tivity. Still, we

need

to

examined

these hypothesis.

CONCLUSION

Based

on

the

_results,

we

_can

EEAfL

increased

the

_immune

rats,

but it

_{had biphasic}_effect_on suggesring

that

_{high dose}

of

EEAfl_

creased non-specific

_immune stead

ofthe

_{specific one.}

REFERENCES

1.. Florentina, _D._{(2013). pengaruh}

Larut

Air

_Kayu_Kuning_{(Arcangelisiel}

(1.) _{Merr.) terhadap}_Fagositosis

tt*

'*,'

33-

u,*

*

-

*-".'

'-

]

i

"t'."

o''."rou,

:';

,r-f

.r,u**

'tL$,:it1--;1i:#

l

s-"r,::i:;,

$;r_li{i:'rli1i+tr*.1r! .

l'.1*,:jil9+!,,

i'

r

(9)

I

6

t6

saftr

e

€r

s

tse

rhc

ffi[

E

fie'

tln

,laY

b

hat

rse

cf

f

le nEl

20131.-d

fu!+5ir

onclttde

respcne

Aft

m-gfn

resporse

aruhr

Ekfd

lngelliia

tosis h|l"ar

Tikus Gatur

hoceeding

@

rcP#p2o*4

tblo

herndionat

Gonf

eneme

qr _{Pharmaceutics & Pharmaceutical Sciences}

Wistar. Yogyakarta: Skripsi

Universitas 4.

Puspitasari, E. and Ulfa, E.U' (20L3). Gadjah Mada. Hal.

39.

Pengembangan Ekstrak Etanol Ar

L

Keawpradub, N., Dej-adisai, A., and

Yue

cangelisia flava Terstandar sebagai Agen nyongsawad, S. (2005).

Antioxidant

Pendamping Kemoterapi Doxorubicin

rd

Cytotoxic Activities of Thai

Medicinal

untuk Pengobatan Kanke; Laporan Ta

NartsNamedKhaminkhruea:Arcangeli

hunanPenelitian,LembagaPenelitian

fr,arra, Coscinium blumeanum

and

Universitas Jember'

illUzrea

_{tinctoria.The}

Songklanakarin

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_{Xu, L',}_Liu,Y., and He, X. (2005)' lnhibi

Jhurqd

of Science and

Technology.2T(2\.

tory

Effects of Berberine on The Action

@27-

and Cell Cycle Progression of Human Pe

f,uran Jakarta (2}1I2l. Peneliti

Temukan

ripheral Lymphocytes' Celluler and Mo

&,ntikanker di Meru Betiri, Koran

Ja

lecular lmmunology- 2(41' 295-3OO'

fngital Edition, http://kora n-jakar