P R O C E E D T N O C B S 2 0 l 3 B O L ' c N , t Y O G Y A K A R T A I S E p T E M B E R 2 0 2 1 2 0 l 3
o-MB-l4
ID:NTIFICATION
OF POLYMORPHISM
ON SIMPLE S;QUEI!C: RsrrATS
MARKERS
ASSOCIATgD
WITH BROWITJ
PLANTHOPPER
R€SISTANCE
GENES IN
TWENTY
RICS GE't]OTYPES
AND THEIIl OENETIC RELA:IONSHIP
Nono
Carsonol',
Yessikha
V Barusa,
Sanflka
Sari5,
Winny D. W darmis,
Danar
Don02,
Yayan
Sumeka13,
and lvlurdaninqsih
H.Kl
I Slalf L€b oi Plant Biotechno ogy antt B a n d r n g S ! m e d a f g 45363 , Stafi, Lab.f Enlono ogy.
a r e e d i( ] . F a c r r t y o f A g r i c ! t L u r e . U n v e r . u t . r s p a d l r . t l . r a n J.lrnangor. E ' m a : i c a r s o n o @ L r n p a d a . i C l A L r r h o r r o f . o r r o s p o n d e n c e ) F a . L r l t y 0 t A g r l c u l u r e , U i i v e . s r l i s ? a d j a d l a r a n J a L f r a . j g o r B a n . i u n g S L r m e d a r g . l 5 3 6 3
ABSTRACT
B r o w n p anthopper i s o n e or ihe mosr deskuctive i n s e c t p e s t oi fire in Indonesra a f . r other Asian c o u n i r i e s . P y r a m i d i n g s o m e b r o w n p r a n t h o p p e r r e s i s t a n c e g e n e s is a va !ab e appfoacf ro cfeare more d L r r a b l e r e s i s i a f c e a g a r m s t t h e p e s t rhe obreclive o f l h i s s r l r c i y w 3 s t o i{ientiay p o i y n r o f p h i s r i s o f B f o w n P l a f t h o p p e r R e s i s t a n c e g e n e s (Bph) on 20 genotypes o i - r o e . a n C to obtatn g e n e t r c r e r r r r o n s h r p a m o n g g e n o t y p e s l e s t e d rrre experirrenl w a s corducted l r o m JLrrre
t o septembef 2012 at Green House and L a b o r a l o r y o f P l a n t A n a l y s t s a n d B olechnology, F a c u t y ofAgrtcrlture. U f i v e r s i i a s p a d l a d l a f a n . J a t i f a n g o r Tw-eniy genoiypes were analyzed, and two oithern were L_rsed
irs cheak varrel es Simple Sequence Repeat ( S S R ) m a r k e r s w e r e applied t o C e t e c i g r r j , Bph| Obph3 and Olrph4 n--nes. Fot;morphrc t e v e t s w e f e a n a l y z e d b y ca.ulat ng plC \polymarphc lnfomatron C o r t e r 0 . The grolrp;Ig o f r i c e g e n o r y p e s
w e r e o o n e D a s e d o n p r i n c i p a l c o m l o n e n t s a n a y s i s ( p C A ) of SSR data. arrcl t h e g e n ; t i c relationship b a s e d on the presence of 8ph geres wa s est me ted us | !l U PG tt/|t\ (.Ltn\'teighted p ai G raup With Atithr letic rneer) Resu)ts s h o w e d l h a t R M 3 T 3 . R l \ 1 8 0 7 2 , R l , 4 8 2 T 3 , R M S 9 S 3 , R l \ 4 5 8 6 , a n d R M 5 8 9 rnafkers w e r e p o t y r . o f p h r c R i c - , g e f o - t y p e s P T B 3 3 . D r a h S u c i . C r b o g o , C t s a n l a f : Dtgut Chera g tnpar 1:l,tnpaf j 0, and Memberarb had Bph3 Qbph1 Bph.1 and Qbpht. t\teanwl )e BphS. AbphS anc:lptt,1 ,ur.]fe s;ppose.t to De beronge( ,y tR 6 4 , A e k S b L r n d o f g B a l a n g c a d i s , R66 anct l \ 4 e k o n g g a l l r i mas nri Tukal penatlr
h a d AphJ. Obphs, d a f Obpr4 lR 74 had Bprsafa Obph3, and Fal ma,,! .,)It h.tt Bph3 and Bphz]. uPGtvtA ctuster ng resu ted rn tvr'o r n a r f custers. in whch lhe firsl cllste. colrsisted o f 2 subclslers prB 33 /as cosey reraiecl w i l h l , 4 e m b e r a n o T u k a t P e r a i L r , D i q u l . D l a h S L c andKairnas.Thessn|.af(ersusedrf:ti.isi|r.tywere!foven
: * " 1 : l : : ! r : l l l : : a r L r F ' ! r i o r ' ' r B p h q e n e s a r d i n e s l m a t i n g g c n e i r . f e l a l r o n s i p s o i r k r e s e n o t y p e s r r b r J W F s a q o o 0 L l o f r o r , o l r e s i s t d . c g c I e s . a s w e t a s l v t e m b e r . I n o T u k : r t p e n a i u . D i g u t , D i a h s L r c t , a n d ^ a r / m a s w r . r t t r L i r c d e ' i i F d r j p r o n ,/ng dofofs tn rice breedjng . e s s t a n c e t o brovii plallL|oppef.
K e y w o r d s : B p r ! e n e . B f o w f p t a n t l t o ! p e r , c e n e l l c fe ationship, S S R mafkers
INTRODUCTIOI\
Indonesia
and the otherAsian
cot]ntries
are currenfly
facing
problems
tn secunng
sup_
plies
of rice.
One reason
of the clecline
in rice
produciion
is the brown
planthoppers
(BpH,
Nilaparuata
lugensStal.
) pests.
BpH is a global
threat
because
not
only
attacK
ng nce
crops
n Indonesia,
but also
attacking
flce crops
ln China,
Thailand,
Vtetnam,
India,
Bangladesh,
l\,4alaysia,
PhiJippines,
Japan,
and Korea
(Baehaki,2007)
Interesting
phenomenon
in the
interaction
betlveen
the rice
varietles
to brown
planthopper
s the appearance
of biotvpe,
the
I Staft,L . r b o J W . o d S c e f . o . F a c ! 1y of Aqrcrtrure !f vcrcitas p . , i t a d t E r a r Jntnango/
I Studenl. A g r o l e c h n o t s g y S I L ' d y
t s a n d ! n g S u m o d a n g , 1 5 3 6 3 .
P r o ! f a n r F a . u l t y o f A g f c! r!re Uriivrys t a s p , r a , a C l a r a n . l . l n a r g o r A : r a i r n g S ! m e d a rg a ti 3 r j t l
j StLrdent l./lastcf
Programi n P r f l Bre.rl fg. FacLriry o f A ! l | l c L i r L r r e . U N , ! e , s t a s p a . l a . l t r i . i n J a l t i a n q n r B a n d ! n i l S r m 0 . t . . g 4 5 3 6 t j
98
r L l t