Content
Conference Report of IBSC2014 Organizing Committee... 1
Committee - National Organizing Committee... 2
- International Advisory Committee... 2
- Scientific Program Committee... 2
- Reviewers………. 3
- Local Organizing Committee………. 4
List of Titles and Authors... 5
Full Proceedings Track 1: Plant Animal and Environmental Science………... 12
Track 2: Engineering and Economy in Agriculture………... 90
Track 3-1: Biotechnology………... 114
Track 3-2: Food Science & Technology... 242
Track 4: Bioactive natural products………... 299
Track 5: Bio-Sensing Technology and Biomaterials………... 375
IBSC2014 proceedings are published in electronic form only.
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Conference Report of IBSC2014 Organizing Committee
On behalf of the organizing committee of The International Bioscience Conference and the 5th Joint international PSU-UNS Bioscience Conference 2014 between the Prince of Songkla University, Thailand and the University of Novi Sad, the Republics of Serbia, the Prince of Songkla University is delighted to host this Conference in 2014. The International Bioscience Conference is organized every two years to enhance the collaboration among various bioscience disciplines and hence help to shape the science of the 21st century together. This year, the 5th International Bioscience Conference 2014 has its main theme “Achieving Sustainability through Integrated Sciences” and takes place on 29-30 September, 2014, in Phuket, Thailand. Top research workers from the Prince of Songkla University, the University of Novi Sad and other research scientists worldwide are gathering to share their experiences of bioscience research.
There are ninety-three papers from both national and international institutes, to share their knowledge. Hopefully, this conference will encourage and inspire young scientists to develop and strengthen their research for the benefits of mankind.
Finally, I would like to express a deep appreciation to our main sponsor the Petroleum Authority of Thailand for their generous support.
Thank you,
Associate Professor Dr. Wilaiwan Chotigeat
Committee
National Organizing Committee
1.Assoc. Prof. Dr. Chusak Limsakul President 2. Prof. Dr. Amonrat Phongdara Vice President 3. Assoc. Prof. Dr. Chutamas Satasook Vice President 4. Assoc. Prof. Dr. Wilaiwan Chotigeat
5. Asst. Prof. Taweesak Niyombandith 6. Asst. Prof. Dr. Sirirat Pinsuwan 7. Assoc. Prof. Dr. Wirote Youravong
8. Assoc. Prof. Dr. Anchana Prathep Secretariat
International Advisory Committee
1. Prof. Dr. Miroslav Vesković President 2. Prof. Dr. Neda Mimica-Dukivić
3. Prof. Dr. Slobodanka Pajević 4. Prof. Dr. Saša Orlović 5. Prof. Dr. Branko Ćupina 6. Prof. Dr. Dubravka Štajner 7. Prof. Dr. Jovan Matović 8. Dr. Anamarija Mandić 9. Dr. Marija Lesjak 10. Miss Gordana Vlahović
Scientific Program Committee
Reviewers
Track 1: Plant Animal and Environmental Science
1. Assoc. Prof. Dr. Anchana Prathep 2. Assoc. Prof. Dr. Pornsilp Pholpunthin 3. Dr. James Dominic True
4. Mr. Kitsanai Charoenjit 5. Dr. Raymond James Ritche 6. Asst. Prof. Dr. Jarun Leerativong 7. Dr. Jaruwat Nabhitabhata 8. Prof. Dr. Slobodanka Pajević
Track 2: Engineering and Economy in Agriculture
1. Dr. Kobchai Worrapimphong
2. Assoc. Prof. Dr. Buncha Somboonsuke 3. Assoc. Prof. Dr. Ayut Nissapa
4. Assoc. Prof. Dr. Yutthana Tirawanichakul 5. Asst. Prof. Dr. Chinnawat Surussavadee 6. Prof. Dr. Branko Ćupina
Track 3: Biotechnology and Food Science & Technology
1. Prof. Dr. Amornrat Phongdara 2. Assoc. Prof. Dr. Wilaiwan Chotigeat 3. Asst. Prof. Dr. Warapond Wanna 4. Dr. Monwadee Wonglapsuwan
5. Assoc. Prof. Dr. Duangporn Kantachote 6. Assoc. Prof. Dr. Prasert Suntinanalert 7. Assoc. Prof. Dr. Nongporn Towatana 8. Assoc. Prof. Dr. Sa-ad Riyajan
9. Assoc. Prof. Dr. Pakamas Chetpattananon 10. Dr. Yanisa Laoong-u-thai
11. Prof. Dr. Soottawat Benjakul 12. Asst. Prof. Dr. Mutita Meenune 13. Assoc. Prof. Dr. Suppasil Maneerat 14. Assoc. Prof. Dr. Wirote Youravong 15. Dr. Supachai Pisuchpen
16. Assoc. Prof. Dr. Aran Hanpongkittikun 17. Prof. Dr. Dubravka Štajner
Track 4: Bioactive natural products
1. Assoc. Prof. Dr. Juraithip Wungsintaweekul 2. Assoc. Prof. Dr. Anuchit Plubrukarn
3. Dr. Sukanya Dej-adisai 4. Asst. Prof. Dr.Sirirat Pinsuwan
5. Assoc. Prof. Dr. Pharkphoom Panichayupakaranant 6. Assoc. Prof. Dr. Roongnapa Srichana
7. Assoc. Prof. Dr. Ruedeekorn Wiwattanapatapee 8. Professor Dr. Vatcharin Rukachaisirikul
Reviewers
Track 5: Bio-Sensing Technology and Biomaterials
1. Assoc. Prof. Dr. Proespichaya Kanatharana 2. Assoc. Prof. Dr. Panote Thavarungkul 3. Asst. Prof. Dr. Waraporn Limbut 4. Asst. Prof. Dr. Chongdee Thammakhet 5. Asst. Prof. Dr. Chittanon Buranachai 6. Dr. Chutintorn Punwong
7. Dr. Apon Numnuam
Local Organizing Committee
1. Asst. Prof. Dr. Pattara Aiyarak 9. Mrs. Nattaya Kanhajaroenpon 2. Asst. Dr. Kamontam Umsakul 10. Miss Janewadee Hiranrat 3. Dr. Phuvadol Thanakiatkrai 11. Miss Kusuma Achirasena
4. Dr. Sureerat Homhuan 12. Mrs. Jittima Phosena
5. Mr. Komwit Surachat 13. Miss Thidawan Saelao
6. Miss Kamontorn Prompitak 14. Miss Wassamon Meesen
7. Miss Nittaya Raknun 15. Mr. Isarapab Chumruksa
List of Titles and Authors
Track 1: Plant Animal and Environmental Science
No. Title Page
T1-I1
Phytoextraction of elevated heavy metals in soil by using fast Growing trees (Salix sp. and Populus sp.)
Authors: Slobodanka Pajević*, Milan Borišev, Nataša Nikolić, Jadranka Luković, Milan Župunski,
Danijela Arsenov, Saša Orlović
13-18
T1-I2 Forest ecosystems in Serbia–monitoring and adaptation
Authors: Saša Orlović*, Zoran Galić, Andrej Pilipović, Srdjan Stojnić, Milan Drekić, Dejan Stojanović
19-22
T1-O4
Physiological and yield responses of yam (Dioscorea rotundata -Poir) vine cuttings to varying rooting chemicals
Authors: Olusola Jacob Oyetunji, Ezekiel Taiwo Afolayan*
23-27
T1-O6 Use of chitosan of shrimp Penaeus sp. as cadmium adsorbent
Authors: Swara Yudhasasmita*
28-30
T1-O7
The use of Mangrove Crab (Scylla sp.) shell chitosan as heavy metal lead (Pb) adsorbent
Authors: Ilma Fistannisa Zette* , Andhika Puspito Nugroho
31-34
T1-O8
Arsenic determination in human hair in Ron Phibun district, Nakhon Si Thammarat province by hydride generation atomic absorption spectrometry
Authors: Nucharee Juansai, Thitima Rujiralai, Wilairat Cheewasedtham*
35-39
T1-O9
Effects of organic fertilizers on uptake and accumulation of cadmium in Merigold (Tagetes erecta L.)
Authors: Natthapong Pechampai, Weeradej Meeinkuirt*, Theerawut Phusantisampan, Jittawan Kubola
40-43
T1-O11 Species Composition and Host Specificity of Fig Wasps in Ficus schwarzii Koord.
Authors: Jirapan Yimkaew*, Chutamas Satasook
44-47
T1-O14
Seaweed and seagrass mapping in Thailand measured by using landsat 8 optical properties
Authors: Satomi Kakuta*, Wataru Takeuchi,Anchana Prathepc
48-52
T1-O21
Vitamin E role as heavy metal (cadmium chloride) antitoxic on albino male rat (Rattus norvegicus, Berkenhout, 1769) hepatic cells
Authors: Alfian Silvia Krisnasari*, Selvi Rahmawati, Qonita Shalihah Firdausyiah, Swara Yudhasasmita, Dita Nurtjahya
53-56
T1-P2
Recruit pattern, mortality and exploitation ratio values of Rhinoclavis sinensis
Gmelin 1791 (Gastropoda: Cerithiidae) from Krakal Beach, Yogyakarta, Indonesia
Authors: Felicia Zahida*, Jusup Subagja, Jesmandt Situmorang, Kamiso H. Nitimulyo
57-60
T1-P6
Photosynthetic performance carbohydrate contents and yield affected by kaolin particle film application in grape
Authors: Teerapat Tepkaew, Sutsawat Duangsrisai, Pilanee Vaithanomsat, Kanapol Jutamanee*
61-65
T1-P7
Induction of polyploid in in vitro shoot of vetiver grass by colchicine for improving salt tolerance
Authors: Mattanaporn Maikami, Lily Kaveeta, Surin Peyachoknagul, Malee Nanakorn*
66-70
T1-P8
Effect of initial pH and alkalinity on hydrogen production from palm oil mill effluent by thermophilic batch fermentation
Authors: Sukonlarat Chanthong*, Sompong O-Thong,Poonsuk Prasertsan
Track 1: Plant Animal and Environmental Science
No. Title Page
T1-P9
Effect of magnetic irrigation on plant height and seed protein of mung bean (Vigna radiata L.) under water stress conditions
Authors: Omid Sadeghipour*
76-78
T1-P13 Screening of RR141 decolorizing bacteria from Khao Yai National Park, Thailand
Authors: Chalermwoot Sompark, Theerachai Thananata, Niramol Sakkayawong*
79-83
T1-P14 Point and non-point sources of pollution of Tisza River (Serbia)
Authors: Srdjan Kolakovic, Svetlana Vujovic*, Borislav Masic, Slobodan Kolakovic,Goran Jeftenic
84-89
Track 2: Engineering and Economy in Agriculture
No. Title Page
T2-01
Contribution of Phytocrene palmata to the Research and Development of plant ingredients for the cosmetic industry
Authors:Eric Gardette*,Pramoth Kheowvongsri, Theerayut Meelap
91-94
T2-02
Effect of sludge pretreatment methods to improve methane production from palm oil mill effluent
Authors:Wiyada Khangkhachit*, Wiriya Duangsuwan, Poonsuk Prasertsan
95-98
T2-03
Potential tuber of wild type banana (Musa acuminata Colla and Musa balbisiana
Colla) for alternative food and bioethanol
Authors: Zanne Sandriati P.*, Ruly Budiono, Moh. Nurzaman
99-102
T2-P1
Potential of heat recovery from air compressor and benefits of its utilization for a greenhouse heating
Authors: Milovan Medojević* , Jovan Petrović, MilanaPerić
103-109
T2-P2
The potential use of agricultural biomass in order to satisfy energy needs of municipal building
Authors: Milana Perić*, Milovan Medojević, Marko Pavlov
110-113
Track 3-1: Biotechnology
No. Title Page
T3-OB4
Isolation and characterization of potential probiotic bacteria for swine feeding
Authors: Uriwan Parneesong, Kornkamol Udomsri, Kesinee Pluamjai, Natchanok Pachara, Kamoltip Promnares*
115-118
T3-OB7
Comparative study of 2,3-butanediol production in C5 and C6 sugar by solventogenic Clostridium spp.
Authors: Chompunuch Glinwong, Jantarush Comwien, Angwara Vudthi, Warawut Chulalaksananukul*
119-122
T3-OB8
Optimizing oleaginous biomass production of Yarrowia lipolytica Po1g for biodiesel feedstock
Authors: Thu T. Phan* , Thien D. Tran, Huong T. Nguyen
123-131
T3-OB9
Optimization of harvesting process and characterization of marine Chlorella sp. biomass
Authors: Yohanis Irenius Mandik, Benjamas Cheirsilp*
Track 3-1: Biotechnology
No. Title Page
T3-OB10
Cultivation of oleaginous fungi Aspergillus tubingensis TSIP9 on palm empty fruit bunches for enzymes and lipid production
Authors: Chonikan Junied, Benjamas Cheirsilp*
137-141
T3-OB11 Cultivation of oleaginous microalgae for carbon dioxide fixation and lipid production
Authors: Tipawan Thawechai, Benjamas Cheirsilp*
142-145
T3-OB12 Optimization of hydrogen production from crude glycerol by Chlorella sp.
Authors: Dennapa Sengmee*, Benjamas Cheirsilp
146-149
T3-OB13 The effect of heat treatment on physical-chemical characteristics of chitosan
Authors: Goragot Supanakorn, Sorada Kanokpanont*
150-153
T3-PB24
Evaluation of oxidative stability and biological activities of liposome-encapsulated curcumin
Authors: Panchika Prangkio*, Supamas Supasri
154-158
T3-PB25
Effect of Phagocytosis Activating Protein (PAP) gene against Aeromonas hydrophila in Cyprinus carpio by oral immunization
Pataraporn Kongmee*, Naraid Saunyuk, Panchalika Dechamag, Wilaiwan Chotigeat*
159-162
T3-PB26
Graphene-conducting polymer nanocomposite modified electrochemical sensor for free radical scavenger screening of ascorbic acid
Authors: Ruksuda Tirawattanakoson, Poomrat Rattanarat, Nadnudda Rodthongkum*, Nattaya Ngamrojanavanich*, Orawon Chailapakul*
163-166
T3-PB27
Physicochemical and antioxidant properties of curry leaves (Murraya koenegii) chutney powder
Authors: Palanivel Ganesan*, Yogeswari Murugan, Anuthida Phaiphan, Badlishah Sham Baharin
167-171
T3-PB28 Characterization of alternative transcript of the 14-3-3 epsilon gene
Authors: Chutintorn Chauywong, Warapond Wanna*
172-175
T3-PB29
The optimization of primary cell culture systems from the ovarian and lymphoid organ of Banana shrimp
Authors: Nathakan Jaturonlak, Wilaiwan Chotigeat*
176-179
T3-PB30 Study of cytokine gene expression during chikungunya virus infection
Authors: Pattra Chalaem, Kamoltip Promnares*
180-183
T3-PB31
Induction of ovarian maturation in Banana shrimp by Emilia sonchifolia (L.) DC. plant extract
Authors: Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat*
184-187
T3-PB32
Conversion of pentoses, hexoses and disaccharide to be acetone-butanol-ethanol (ABE) by Clostridium sp. Hdi
Authors: Supattra Lertsriwong, Nassapat Boonvitthya, Warawut Chulaluksananukul, Chompunuch Glinwong*
188-192
T3-PB33
Screening of lipase-producing thermophilic bacteria from hot springs in northern Thailand
Authors: Sukumarn Kittivisarn, Tikamporn Yongvanich, Warawut Chulalaksananukul*
193-197
T3-PB34
Optimization of biodiesel production by mutant catalytic cells of Candida orthopsilosis from crude palm oil using Response Surface Methodology
Authors: Wannisa Pansuk, Marisa Jatupornpiput, Aree Rittiboon*
198-202
T3-PB35 Functional analysis of shrimp 14-3-3 protein involved in apoptosis
Authors: Kittisak Thongpijit, Warapond Wanna*
Track 3-1: Biotechnology
No. Title Page
T3-PB36
Adsorption of iron(III) and copper(II) by bacterial cellulose produced from
Rhodococcus sp. MI 2
Authors: Pariyaporn Yingkong, Somporn Tanskul*
207-210
T3-PB37 Dust mites detection by multiplex PCR
Authors: Thanyarat Teerawattananon, Sirinthra Rittipong, Thatsanee Thet-Em, Nat Malainual*
211-214
T3-PB38 Using crude glycerol for growth and lipid production by Mortierella elongata NR06
Authors: Supavadee deelai, Panida Unagul, Niramol Sakkayawong*
215-218
T3-PB39 Polyhydroxyalkanoates production from municipal wastes by Bacillus licheniformis
Authors: Kanokphorn Sangkharak*, Poonsuk Prasertsan
219-221
T3-PB40
The production of cholesterol-lowering agent (mevastatin) by solid-state cultivation of Monascus sp. SS14
Authors: Pharita Thongsuknok, Somchai Krairak*
222-225
T3-PB41 Structural analysis and heterologous expression of human ROBO1
Authors: Kanokporn Boonsirichai*, Suwimol Jetawattana
226-229
T3-PB42
Design and testing of long throat venturi- and jet-type bubble generators for using in palm oil industry
Authors: Wiriya Duangsuwan*, Poonsuk Prasertsan, Annop Chawalitsittikul
230-233
T3-PB43
Detection of oil palm variety tenera by Loop-Mediated Isothermal Amplification Method (LAMP)
Authors: Sarawut wannapat*, Alisa Nakkaew, Amornrat Phongdara
234-237
T3-PB44 Development of a simple test for detecting the White Spot Syndrome Virus (WSSV)
Authors: Nittaya Bunsanong, Benyapa Intharasiri, Piyaporn Khunthongkae, Suchera Thananimit*
238-241
Track 3-2: Food Science & Technology
No. Title Page
T3-IF1 Antioxidants in Serbian functional foods
Authors:Dubravka Štajner*, Boris M. Popović, Jasna Čanadanović-Brunet, Nataša Milić
243-246
T3-OF6
Feasibility of utilizing skimmilk powder and whey protein powder in Greek-style yogurt production
Authors:Chanokphat Phadungath*
247-250
T3-OF7 Development of seaweed sorbet using Kappaphycus alvarezii
Authors:Liyana Mohammed*, Sharifudin Md Shaarani
251-255
T3-OF8
Nutritional composition, antioxidant activity and total phenolic content of bread incorporated with seaweed (Sargassum Polycystum sp.)
Authors:Haini N*, Matanjun P, Song T.T, Yasmin O.B.H
256-262
T3-OF9
Histopathological alterations and antioxidant enzymes activities in organs of hyperlipidaemic rats fed red seaweed, Gracilaria changii powder
Authors:Pei Teng Chan*, Patricia Matanjun, Suhaimi Md Yasir, Tek Song Tan
263-269
T3-OF10 Pomergranate seed oil encapsulation in the zein nanoparticles
Authors:Hamid Tavakolipour*, Ashkan Madadlou, Mohsen Mokhtarian
Track 3-2: Food Science & Technology
No. Title Page
T3-PF7
Characterization of digestive enzyme extracted from Nile tilapia (Oreochromis niloticus) Viscera
Authors:Tatchaporn Chaijaroen*, Chakree Thongraung
274-277
T3-PF8
Effect of sodium alginate coating on product characteristics, water loss, solid gain, ascorbic acid degradation during the osmotic dehydration of guava
Authors:Opasinee Kongmaneepitak, Saowanee Lertworasirikul*
278-281
T3-PF9
Effect of sorbitol stress on polyamine content and agmatinase activity in wild type and ∆speB2 mutant of Synechocystis sp. PCC 6803
Authors:Supapid Eknikom, Aran Incharoensakdi, Saowarath Jantaro*
282-285
T3-PF10 Characterization of GABA-producing probiotic isolated from Thai fermented foods
Authors:Nantana Yuthaworawit*, Chalat Santivarangkna*
286-289
T3-PF11
Polyhydroxybutyrate (PHB) content and its biosynthetic gene expression in cyanobacterium Synechosystis sp. PCC 6803 under mineral nutrient conditions
Authors:Suthira Utharn, Aran Incharoensakdi, Saowarath Jantaro*
290-293
T3-PF12
Molecular characterization of estrogen receptor gene in the black tiger shrimp (Penaeus monodon)
Authors:Sripapan Tharanat, Natthaporn Khwanmuang, Rachanimuk Hiransuchalert*
294-298
Track 4: Bioactive natural products
No. Title Page
PL-2 Native plants in Serbia-Opportunity for new herbal drugs
Authors:Neda Mimica-Dukić*, Ivana Beara, Marija Lesjak, Emilija Jovin, Nataša Simin
300-304
T4-O2
Protective role of N-trans-feruloyltyramine in hydrogen peroxide-induced cell death
Authors:Ei Ei Phyo Myint, Rungtip Soi-ampornkul, Wanphen Katanyoo, Wipawan Thangnipon, Patoomratana Tuchinda, Saksit Nobsathian, Chalermchai Mitrpant*
305-309
T4-O3
Extraction and determination the effectivess of teh-tehan leaf (Acalypha siamensis) against Staphylococcus aureus bacteria
Authors:Anggraeni Wulandari*, Nikmahtul Eviana, Reni Desiriana*, Arniz Hanifa, Munji Lestari
310-312
T4-O5 Anti-HIV proteins from two varieties of Momordica charantia cultivated in Thailand
Authors:Wichet Leelamanit*, Thitidaj Luetrakull
313-314
T4-P3
Setting time, surface pH and fluoride release of novel chitosan modified glass-ionomer cement
Authors:Sareepah Manmoo*, Ureporn Kedjarune-Leggat, Chanothai Hengtrakool
315-318
T4-P4
Effects of Musa sapientum extract on biofilm formation and acidogenicity of
Streptococcus mutans
Authors:Uraiwan Sungchum*, Sissada Tannukit, Suwanna Jitpukdeebodintra
319-322
T4-P5
Antimicrobial activities of Zingiberaceae extracts against fungi producing aflatoxin isolated from stored rice seeds and grains of Khoa Dawk Mali 105
Authors:
Puntip Tan-a-ram*, Pituk Maolee, Anusara Janklang, Anuchida Chokmongkon, Oratai Faina
Track 4: Bioactive natural products
No. Title Page
T4-P6 Effect of degree of hydrolysis on the antioxidant activities of Skipjack tuna skin
Authors:Worrapanit Chansuwan, Pavinee Chinachoti*
328-332
T4-P7
-Glucosidase inhibitors from Java tea Orthosiphon aristatus (Blume) Miq.
Authors:Doungkamon Toumsuk, Preecha Phuwapraisirisan*
333-337
T4-P8 Anti-HIV-1 integrase activity of Albizia procera (Roxb.) Benth bark
Authors:Pattarapan Panthong, Chatchai Wattanapiromsakul, Supinya Tewtrakul*
338-339
T4-P9
Antioxidant and antimicrobial activities of Delonix regia (Bojer) Raf. Extracts
Authors:Supakorn Boonyuen*, Niramol Sakayawong, Pariya Na Nakorn, Thanatphat phetcharat, Kamonthip Mungkalasawat
340-344
T4-P10 Biological activities of Aquilaria crassna leaf extracts
Authors:Tipakorn Mungprom*, Kanokporn Boonsirichai, Wichet Leelamanit
345-348
T4-P11 Biological activities of Momordica cochinchinensis oil seed extract
Authors:Nhunpong Daechacupt*, Wichet Leelamanit
349-352
T4-P12 Effects of polymer coating on drug granules for controlling drug delivery systems
Authors:Boonyong Punantapong*, Sudarath Suntaropas
353-357
T4-P13
A glucose/mannose-specific lectin with alpha-glucosidase inhibitory activity from
Sterculia monosperma Vent seeds
Authors:Aphichart Karnchanatat*, Polkit Sangvanich
358-362
T4-P14
Effects of ethanolic extracts of some plants of Zingiberaceae and Lamiaceae on the growth of pathogenic and probiotic bacteria
Authors:Monton Visutthi*
363-365
T4-P15
Metabolite profiles of two cultivars of Thai rice Oryza sativa L. that are different in salt tolerance
Authors:Kwankao Karnpakdee*
366-369
T4-P16
Screening of potential anti-fungal enzymatic activity in elicited callus culture of Drumstrick tree (Moringa oleifera Lam.)
Authors:Wanutchaya Duanginta, Srisulak Dheeranupattana, Nopakarn Chandet, Lalida Shank*
370-374
Track 5: Bio-Sensing Technology and Biomaterials
No. Title Page
T5-O1
Raman spectroscopy: A new alternative tool for food safety
Authors:Philippe Daniel*, Fabienne Lagarde-Abribat, Jean-François Pilard, Gérald Thouand, Pranee Phinyocheep
376-379
T5-O2 Preliminary results of texture analysis in breast cancer microscopic image
Authors:Sirinapa Jitaree, Pornchai Phukpattaranont*
380-383
T5-P4
Graphene/polyaniline nanocomposite modified electrode for biosensors
Authors:JutipornYukird, Ratthapol Rangkupan, Trairak Pisitkun, Nadnudda Rodthongkum*
, Orawon Chailapakul*
Track 5: Bio-Sensing Technology and Biomaterials
No. Title Page
T5-P5 Colorimetric detection of trinitrotoluene via glutathione-capped silver nanaporticles
Authors:Pichaya Wilaivarangkul, Panote Thavarungkul, Proespichaya Kanatharana, Warakorn Limbut*
388-392
T5-P6
Cryogel entrapped acetylacetone sol-gel sensor for formalin detection
Authors:Lapasrada Aksornneam, Proespichaya Kanatharana, Panote Thavarungkul, Chongdee Thammakhet*
393-396
T5-P7 Mixed-mode cation exchange for the extraction of -agonists
Authors:Supattri Noosang, Opas Bunkoed, Panote Thavarungkul, Proespichaya Kanatharana*
397-400
T5-P8
Electrochemical detection of trinitrotoluene using a poly(1,3,5-triazine-2,4,6-triamine) modified glassy carbon electrode
Authors:Suparat Cotchim, Sirinun Kanjun, Panote Thavarungkul,Proespichaya Kanatharana, Warakorn Limbut*
401-404
T5-P9
Bismuth-modified poly(glutamic acid)/glassy carbon electrode for zinc detection by anodic stripping voltammetry
Authors:Anisah Dueraning, Proespichaya Kanatharana, Panote Thavarungkul, Warakorn Limbut*
405-409
T5-P10
Electrochemical sensor for carbaryl detection based on ordered mesoporous carbon modified electrode
Authors:Jiranan Sangduangyang, Panote Thavarungkul, Proespichaya Kanatharana, Apon Numnuam*
410-414
T5-P11
Silver nanodendrite/carbon nanotubes modified electrode for hydrogen peroxide detection
Authors:Jittima Choosang, Proespichaya Kanatharana, Panote Thavarungkul, Apon Numnuam*
415-419
T5-P12
Extracted substances of mangosteen pericarps from methanol extraction and their monomer types analyzed from UPLC/MS
Authors:Narita Khundamri, Chahinez Aouf, Hélène Fulcrand, Eric Dubreucq, Varaporn Tanrattanakul*
420-423
T5-P13
Synthesis of waterborne polyurethane containing natural rubber and poly(ethylene glycol)
Authors:Kanokporn Puttaro*, Chuanpit Khaokong
T1-O7
The use of Mangrove Crab (
Scylla
sp.) shell chitosan as heavy metal lead
(Pb) adsorbent
Ilma Fistannisa Zette
a,* , Andhika Puspito Nugroho
aa
Faculty of Biology, University Gadjah Meda, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia 55281
*Corresponding author. Tel: +6281215521422.
E-mail address: ilmazette@ugm.ac.id
Abstract. Heavy metal pollution of lead (Pb) in the environment can be addressed by lowering the concentration of Pb using adsorption techniques. Chitosan is a biopolymer chitin deacetylation modifies it into a potential heavy metal adsorbent. One source is the chitosan shell from mud crab (Scylla sp.) common as household eating waste. This study aims to determine the efficiency of the use of mud crab shells as raw material for chitosan and to determine the most optimal chitosan concentration and stirring time for the adsorption of Pb. Isolation of chitin done with deproteination and demineralization of mud crab shell powder. Chitosan is obtained from chitin deacetylation. Adsorption by chitosan performed on 27 samples of 1 ppm Pb 10 ml with chitosan concentration variation of 0.1;0.3;0.5 grams and stirring time for 5;10;15 minutes. Determination of heavy metal content in the samples was done using Atomic Absorption Spectrophotometer (AAS). The results showed that the efficiency of the mangrove crab shell chitosan as raw materials amounted to 29.67%. The most optimal chitosan concentration and stirring time for 1 ppm Pb adsorption is 0.5 grams chitosan with 15 minutes stirring time.
Keywords: chitosan; lead; Scylla sp.
1. Introduction
Heavy metal pollution especially in water is very harmful to organisms. Heavy metals have toxic effects that influence biological processes and even can cause death. This is because heavy metals are not able to be broken down through biological processes and tend to settle to the bottom of waters associated with organic compounds in the sediment. Various cases of heavy metal pollution affects humans. One of the first known cases of the world is the death of cats and crows in Minamata Bay area, Japan and unexplainable disease among some residents of surrounding Jintsu River. They suffered from mercury poisoning from the Chisso factory wastewater (Iyoda, 2010). Heavy metals into the aquatic environment is usually derived from industrial waste. Concentration increases due to continuous discharge unchecked will degrade water quality (Banerjee, 2002). One of the heavy metals is lead (Pb) which is one of the most dangerous heavy metals for human health (Scoullos et al., 2001).
Lead is a heavy metal that is bluish-gray. Lead is present in nature in rocks, soil, and vegetation. While lead is released as a result from mining, smelting, refining, and secondary reprocessing (Anies, 2006). Lead is used as a material for producing products such as ammunition, wire coatings, pipes and solder, chemicals, dyes, and others. However, lead is most widely used to manufacture batteries. In addition in the form of particles, lead also we often encounter in the form of gas (metal fumes). Gases that pollute the air with lead is largely tetraethyl and tetramethyl-Pb-Pb derived from the combustion of motor vehicle gasoline additives (Fardiaz, 1992).
To overcome Pb pollution caused by industry requires an effort. Efforts are made to reduce the concentration of pollutants to below the threshold of environmental standards before being discharged into the environment. The method that is themost inexpensive and practical is an adsorption technique using a low-cost adsorbent such as seafood processing waste (Umaningrum et al., 2010). Waste crab shell waste is one that can be used as an adsorbent for heavy metal Pb. Chitin can be converted to chitosan by a deacetylation process. Chitosan is able to absorb heavy metal ions cations effectively because it has a hydroxyl group and an amino along the polymer chain (Sanjaya and Yuanita, 2007).
In the manufacture of chitosan, there are three main processes deproteinisation, demineralization, and deacetylation. Deproteinisation and demineralization is achieved by using a solution of acids and bases, to remove proteins and minerals in order to obtain purified chitin. Chitin is then converted into chitosan by deacetylation by heating in an alkaline solution (Rege and Lawrence, 1999; Tolaimatea, et al., 2003)
Mangrove crabs are crabs that live in coastal waters, particularly in the mangrove forests (mangroves). Indonesia is known as a large exporter of crabs. Mangrove crab (Scylla sp.) is one of Indonesia’s fishery commodities that is in demand both at home and abroad. Consumption is high due to their delicious taste and are highly nutritious. However, the waste generated from the consumption of shell mud crab cannot be used optimally. Mangrove crab shells contain chitin and are readily available, therefore we have studied the adsorption of chitosan from mangrove crab shells against one particularly dangerous heavy metal, namely lead (Pb).
Based on information from a variety of references, the efficiency of mud crab shells as a source of chitosan is 50-60%. In addition, the concentration and the duration of stirring in the adsorption stage metal lead (Pb) by chitosan will affect a decrease in metal content in the solution, ie the higher the concentration and stirring time, the adsorption of Pb will be even greater. This study aims to determine the efficiency of mud crab shells as raw material for chitosan and to determine the most optimum chitosan concentration and stirring time for the adsorption of Pb.
2. Materials and Methods
The mud crab shells were collected from a local market in Pati, Central Java, Indonesia. The viscera and tissues were carefully removed. The shells were washed and dried with no sun light. The sample was then deproteinized with 3% NaOH at a ratio of 1:6. The mixture was heated to 80-85 °C for 30 min. After it was filtered, the residue was washed with distilled water until no longer alkaline. The powder was dried in an oven at a temperature of 70-80 °C for 24 hours. Crab shell powder that has undergone deproteinisation was then demineralized at 1.25 N HCl in the ratio 1:10. This mixture is stirred using a magnetic stirrer for 2 hours. The residue was heated in a temperature of 70-75 °C for 1 hour. The resulting product ischitin in the form of a pale cream-colored powder. Chitin was deacetylated with 50% NaOH at a ratio of 1:10 and then heated at a temperature of 95-100 °C for 30 min. Chitosan powder obtained was washed with distilled water until neutral, then filtered and dried in an oven at a temperature of 80 °C for 24 hours. The chitosan yield was calculated by comparing the weight measurements of the raw material to the chitosan obtained after treatment. Adsorption by chitosan performed on 27 samples of 1 ppm Pb 10 ml with chitosan concentration variation of 0.1;0.3;0.5 grams and stirring time for 5;10;15 minutes. The results were quantitatively and qualitatively analyzed. Quantitative comparison of the data was Pb concentration of the control and treatment group that analyzed using Atomic Absorption Spectrophotometry. The qualitative data were analyzed by one way ANOVA and Tukey HSD test.
3. Results and Discussion
Pb concentration in each treatment was significance tested and the results indicated that there was significant difference between each treatment. The data presented in the graph (fig.1) shows the correlation between time stirring with a decrease in the concentration of Pb. In addition to the effect of different chitosan concentrations the decrease in the concentration of Pb is also different.
Figure 1 shows the effect of chitosan on the Pb concentration. Control treatment did not alter the concentration of 1 ppm Pb treatment while using chitosan is able to decrease the concentration of chitosan. Sequentially the biggest drop was in 0.5 g chitosan followed by 0.3 grams and 0.1 grams. In each chitosan concentration decreased Pb concentration and binding increased over stirring time, so that the most significant time decrease the concentration of Pb is 15 minutes. Overall, the most optimal treatment lowers the concentration of Pb is chitosan concentration of 0.5 g for 15 min stirring time, amounting to 93.6% completion of absorption
Table 1. The efficiency of mud crab shell as raw material of chitosan. Weight of mud crab shell
powder
Weight of chitosan yield Efficiency
30 gram 8,9 gram 29,67%
The use of mud crab shells as raw material chitosan is an effort in the utilization of the waste feed of crab shells. From this study the efficiency of the mangrove crab shell chitosan as raw material for absorption is relatively low (29.67%) (Table1). The accuracy of the method can affect the weight of the resulting chitosan. Deacetylation reaction of chitin into chitosan is influenced by many factors that most chitin may not be perfectly transformed into chitosan.
The ability of chitosan as an adsorbent of heavy metals, particularly Pb characterized by a decrease in the concentration of Pb in solution. In this study 1 ppm Pb concentration decreased with the addition of chitosan (Figure 1). The higher the concentration of chitosan used, the greater the reduction in Pb concentration. The largest concentration of chitosan, which resulted in a decrease of 0.5 grams of the greatest concentrations of Pb. While the smallest chitosan concentration (0.1 grams), resulted in the lowest decrease in the concentration of Pb smallest. Chitosan has electrons of nitrogen in the amine group capable of covalent bonds with transition metal ions. As an electron donor, the concentration of chitosan affects the number of electrons available. The more electrons are available, the more covalent bonds are formed with metal ions. The bond formed in chitosan-metal complexes that can reduce the effects of environmental pollutants such as Pb (Suharsih, 2008).
In an adsorption process, influencing factors, among others, the stirring speed and contact time. Stirring speed will determine the speed of the adsorption process, while the contact time can produce the maximum adsorption capacity (Syauqiah et al., 2011). The longer the contact time, the greater the adsorption. From this research it has been found that the contact time of 15 minutes is an optimal time in Pb heavy metal adsorption by chitosan (Figure 1). It is not known whether this time is a time of equilibrium that produces the maximum adsorption capacity. Thus need to conduct further research with a wider range of time.
4. Conclusions
The efficiency of the mangrove crab shell as raw materials of chitosan amounted to a yield of 29.67 %. The greater the concentration of chitosan and long stirring time, the greater the concentration of Pb will decrease .The most optimal chitosan concentration and stirring time for 1 ppm Pb adsorption is 0.5 grams chitosan with 15 minutes stirring time.
5. Acknowledgements
The authors gratefully acknowledge the contributions of Faculty of Biology UGM and LPPT UGM for supporting the research.
6. Conflict of interest
Conflict of interest: none.
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