A new strategy to stabilize oxytocin in aqueous solutions: i. the effects of divalentt metal ion and citrate buffer.
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![Fig. 1. Recovery of oxytocin in the presence of divalent metal ions inRP-HPLC.The results are depicted as averages of three independentconcentrations of 2, 5, 10, and 50 mM.non-buffered, pure water stored for 4 weeks at pH 4.5 and atemperature of 4°C (ligh](https://thumb-ap.123doks.com/thumbv2/123dok/948527.618375/3.595.53.295.240.650/recovery-oxytocin-presence-divalent-depicted-independentconcentrations-buffered-atemperature.webp)
![Fig. 2. Recovery of oxytocin in pure water, with or without amonomer recovery determined by HP-SEC](https://thumb-ap.123doks.com/thumbv2/123dok/948527.618375/4.595.243.534.49.486/fig-recovery-oxytocin-pure-water-amonomer-recovery-determined.webp)
![Fig. 4. Oxytocin recovery over time storage at 40°C and pH 4.5 in thesymbolsin concentrations of 10 mM (recovery determined by HP-SEC](https://thumb-ap.123doks.com/thumbv2/123dok/948527.618375/5.595.52.289.228.658/fig-oxytocin-recovery-storage-thesymbolsin-concentrations-recovery-determined.webp)
![Table I. Thermodynamics of Divalent Metal binding to Oxytocin as Determined by Isothermal Titration Calorimetry in 10 mM Citrate Buffer](https://thumb-ap.123doks.com/thumbv2/123dok/948527.618375/6.595.79.511.464.691/thermodynamics-divalent-oxytocin-determined-isothermal-titration-calorimetry-citrate.webp)
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