i
i ABSTRAK
Lailasari Hutabarat, Pemberian Beberapa Jenis Antioksidan terhadap Peningkatan Ketahanan Salinitas pada Turunan F4 Kedelai Berdasarkan Aktivitas
Enzin Peroksidase (POD) dan Superoksida Dismutase (SOD). Dibimbing oleh Dr. Diana Sofia Hanfiah, SP., MP. dan Prof. Dr. Ir. Rosmayati, MS.
Peningkatan produksi kedelai dapat dilakukan dengan memanfaatan lahan marginal seperti lahan salin melalui pemanfaatan genotipe hasil persilangan antara toleran salin dengan berproduksi tinggi dan pemberian beberapa antioksidan yaitu asam askorbat, asam salisilat, dan α-tokoferol untuk melindungi sel-sel tanaman dari efek kondisi stres garam. Penelitian dilaksanakan di rumah plastik di lahan percobaan Fakultas Pertanian Universitas Sumatera Utara, analisis karakter fisiologi dan biokimia tanaman di Laboratorium Kultur Jaringan Tanaman USU, Balai Penelitian Sungei Putih, Laboratorium Sentral USU bulan Februari sampai Agustus 2016. Penelitian menggunakan Rancangan Acak Kelompok Satu Faktor yaitu perlakuan antioksidan dan perlakuan diulang dua kali.
Hasil analisis data menunjukkan bahwa perlakuan antioksidan tidak memberikan pengaruh yang nyata terhadap peningkatan ketahanan salinitas pada turunan F4 kedelai. Secara deskriptif perlakuan asam askorbat 750 ppm memberikan rataan tertinggi terhadap klorofil daun dan bobot biji per tanaman, perlakuan asam salisilat 250 tertinggi pada enzim SOD, dan perlakuan asam salisilat 500 ppm tertinggi pada enzim POD.
Kata kunci : antioksidan, salinitas, POD, SOD, kedelai
ii
ii ABSTRACT
Lailasari Hutabarat, Giving various type of antioxidant to Increase Resilience
Salinity on Enzyme Activity Based F4 Soybean peroxidase (POD) and superoxide
dismutase (SOD). Supervisied by Dr. Diana Sofia Hanfiah, SP., MP. and
Prof. Dr. Ir. Rosmayati, MS.
The production of soybean can be increased through a marginal land use such as saline land, using genotype as the result of crossing the high-production tolerant saline and giving various of antioxidant, such as ascorbic acid, salicylic acid, and alpha-tocopherol to protect the plant cells from the effect salt-stress. This research carried out in the plastic house in experimental field of Faculty of Agriculture University of North Sumatera, physiology and biochemistry analysis in Plant Tissue Culture Laboratory University of North Sumatera, Sungei Putih Research Center and Central Laboratory of University of North Sumatera on February – August 2016. And used factorial Randomized Block Design with one factor were antioxidant and two replications.
The results showed that antioxidants not significantly effect to the increase of tolerance salinity in the F4 generation of soybean. Descriptively, 750 ppm of ascorbic acid treatment has given the highest average to chlorophyll analysis and weight of seeds per sample, the highest treatment of 250 ppm of salicylic acid was to SOD enzyme, and the highest treatment of 500 ppm of salicylic acid was to POD enzyme.
Keywords : antioxidant, salinity, POD, SOD, soybean
