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LAMPIRAN A
DIAGRAM ALIR PENELITIAN
A.1. Diagram alir pemisahan tahap I menggunakan metode SEC dan PAGE
PEMISAHAN DENGAN KOLOM SEPHADEX G-25 M CAIRAN FLOEM PEMISAHAN PAGE PEMISAHAN SEC ANALISIS/INTERPRETASI DATA PENDETEKSIAN UNSUR (ICP QMS) PEMISAHAN DENGAN KOLOM SEPHADEX G-50 SF Eluent (20mM + NaN31mM pH 8,0): (1) Tris-HCl (2) MES (3) NaCl PENDETEKSIAN UV VIS PENENTUAN KONSENTRASI PROTEIN/ POLIPEPTIDA Eluent: MES 20 Mm / NaN31mM pH 8,0 PEMISAHAN DENGAN KOLOM SEPHADEX G-25 M CAIRAN FLOEM PEMISAHAN PAGE PEMISAHAN SEC ANALISIS/INTERPRETASI DATA PENDETEKSIAN UNSUR (ICP QMS) PEMISAHAN DENGAN KOLOM SEPHADEX G-50 SF Eluent (20mM + NaN31mM pH 8,0): (1) Tris-HCl (2) MES (3) NaCl PENDETEKSIAN UV VIS PENENTUAN KONSENTRASI PROTEIN/ POLIPEPTIDA Eluent: MES 20 Mm / NaN31mM pH 8,0
A.2. Diagram alir pemisahan tahap II spesi Mo menggunakan metode SEC dan PAGE PEMISAHAN PAGE PEMISAHAN SEC ANALISIS/INTERPRETASI DATA PENDETEKSIAN UNSUR (ICP QMS) PENDETEKSIAN UV VIS Eluent: MES 20 Mm / NaN3(1mM) pH 8,0 Fraksi SEC kolom Sephadex G-50 SF Fraksi PAGE PEMISAHAN PAGE PEMISAHAN SEC ANALISIS/INTERPRETASI DATA PENDETEKSIAN UNSUR (ICP QMS) PENDETEKSIAN UV VIS Eluent: MES 20 Mm / NaN3(1mM) pH 8,0 Fraksi SEC kolom Sephadex G-50 SF Fraksi PAGE
A.3. Diagram alir pemisahan tahap II spesi Ca menggunakan metode CE CE ANALISIS/INTERPRETASI DATA PENDETEKSIAN UV VIS Optimasi metode pH
Tegangan Temperatur Waktu analisis
Waktu injeksi Konsentrasi BGE BGE: (1) Tris-HCl (2) Na2HPO4/ NaH2PO4/CTAB (3) H3BO3/CTAB Fraksi SEC kolom Sephadex G-25 M CE ANALISIS/INTERPRETASI DATA PENDETEKSIAN UV VIS Optimasi metode pH
Tegangan Temperatur Waktu analisis
Waktu injeksi Konsentrasi BGE BGE: (1) Tris-HCl (2) Na2HPO4/ NaH2PO4/CTAB (3) H3BO3/CTAB Fraksi SEC kolom Sephadex G-25 M
LAMPIRAN B
PENGKULTURAN TANAMAN JARAK
B. 1. Rumah kaca tempat pelaksanaan kultur tanaman jarak
Gambar B.1. Rumah kaca “PhyTec”, Institute for Chemistry and Dynamics of the Geosphere: ICG-III Phytosphere, Research Center Jülich,Jülich, Jerman.
Gambar B.2. Kultur tanaman dilakukan pada rumah kaca “PhyTec”, Institute for Chemistry and Dynamics of the Geosphere: ICG-III Phytosphere, Research Center Jülich, Jülich, Jerman. Biji jarak (Ricinus communis L.) diperoleh dari perusahaan pembibitan terakreditasi Jelitto Staudensamen GmbH (Hamburg, Jerman).
B.2. Pengambilan cairan floem tanaman jarak
LAMPIRAN C
RANGKAIAN ALAT SEC KOLOM SEPHADEX G-50 DAN ICP-QMS
C.1 Rangkaian Alat SEC
Kolom sephadex G-50 Detektor UV Pengumpul fraksi Pemograman alat Kolom sephadex G-50 Detektor UV Pengumpul fraksi Pemograman alat
LAMPIRAN D
BAGAN ALAT PNC PAGE MODEL 491 PREP CELL
Buffer elusi Buffer elektroforesis atas Sistem pendingin Pita protein Ruang elusi Tabung gel Buffer elektroforesis bawah Tabung pengumpul elusi Buffer elusi Buffer elektroforesis atas Sistem pendingin Pita protein Ruang elusi Tabung gel Buffer elektroforesis bawah Tabung pengumpul elusi
LAMPIRAN E
PENGARUH UMUR TANAMAN TERHADAP VOLUME CAIRAN FLOEM YANG DIHASILKAN
Jumlah cairan floem/tanaman (µL) Umur tanaman (pekan) 1 2 3 rerata SD 5 61 51 13 42 25 6 19 52 61 44 22 7 82 89 22 64 37 8 80 20 72 57 33 9 19 64 76 53 30 10 85 45 40 57 25
LAMPIRAN F
DATA HASIL OPTIMASI HARIAN ICP-QMS Analit
Noise pada m/z
Nomor analisis
Li Mg Rh Pb U Ba BaO Ba++ Ce CeO 5 220 260
1 29584 93907 352241 259212 469452 386062 302 9511 474593 13255 12 12 13 2 30776 98884 360447 274022 528863 402173 271 9312 487008 12997 14 12 14 3 30089 98108 360816 273661 530816 403459 275 9398 488734 12837 13 14 14 4 40281 131169 470628 422288 810750 524030 557 13405 635131 19913 4 4 4 5 37226 112090 402947 293024 560551 450122 329 10714 545406 14912 12 13 14 6 2871 9066 28512 18852 34888 23235 43 623 30082 822 16 18 17 7 28137 102848 430924 311598 588691 486212 369 11299 592593 16704 13 13 12 8 35460 112730 420726 303337 578765 467488 348 11014 570101 15806 12 13 11 9 34572 115522 398069 284025 539091 448285 318 11356 545520 15049 12 11 12 10 36552 125615 457164 327226 622243 518212 383 13527 630935 18943 9 12 10 11 34534 124292 456712 323162 616839 515751 437 13978 628051 19217 9 12 11 12 30991 111533 403809 292096 546563 465984 360 11380 570463 16380 11 13 11 13 29303 115036 440945 314443 592917 486187 362 12657 588236 16685 10 12 13 14 41030 129426 398711 269457 499295 431632 367 13080 527025 14757 10 14 12 15 36266 133797 427883 294350 555708 467440 340 13683 571474 16019 10 12 14 16 33746 127455 444132 303717 548736 487447 409 13751 590078 17722 15 18 16 17 32118 133245 457160 317819 591245 503883 362 14571 611703 16872 15 18 15 18 36980 135490 434195 305019 573275 482088 358 13265 583837 16356 15 17 18 19 26935 115176 385560 263666 493060 418187 347 12266 509287 15155 18 23 19 20 36011 129935 437047 310718 576436 489401 423 13950 589019 16629 20 25 25 21 36440 124971 555495 391744 762062 573212 542 13737 707323 18995 5 5 6 22 24676 102480 486389 321165 628177 489934 471 12744 610354 17585 3 4 5
DATA HASIL OPTIMASI HARIAN ICP-QMS (lanjutan) Analit
Noise pada m/z
Nomor analisis
Li Mg Rh Pb U Ba BaO Ba++ Ce CeO 5 220 260
23 30062 115286 524106 358045 706241 538834 447 12769 660482 17090 3 6 4 24 26174 108255 550213 374438 725692 573197 513 12646 701580 18233 5 6 6 25 29575 118105 590359 414015 802399 610733 514 14084 750092 19058 4 6 6 26 26615 110624 500990 342282 662389 514839 456 14119 631400 17417 3 4 3 27 28517 116839 538463 359833 696105 536167 509 14193 663279 19006 3 4 2 28 31876 118377 489548 332216 651997 499662 476 13158 620520 17476 3 3 3 29 26860 99783 405873 281000 545821 412823 472 11045 511312 14215 3 3 3 30 35053 120134 474288 339496 663896 498309 490 14337 610306 16838 2 2 2 31 23084 91621 437987 311188 612388 456088 406 11671 560662 14098 3 3 3 32 36153 124387 496955 373180 728513 529947 523 12623 642926 18737 3 3 3 33 35594 122414 523799 404313 788508 564096 495 12362 684770 17477 2 4 5 34 29356 114245 484532 369288 716671 519817 507 12839 634391 17720 2 3 3 35 32516 122386 552768 431802 832779 595459 548 14954 718411 20218 2 3 2 36 31312 118462 509019 387631 749845 546477 497 14044 667471 17873 3 3 2 37 29188 114747 456604 339763 659593 482538 475 13195 596975 17045 2 2 2 38 31172 119252 447771 336588 651626 475087 482 12759 584735 16924 2 2 2 39 29065 105412 399842 290527 564287 421690 426 11211 521902 14688 1 2 2 40 23937 92510 389248 289159 560876 413696 413 10676 512216 13545 2 3 2 41 38140 136826 448587 408126 785916 495973 486 12634 608725 16087 4 4 4 42 30508 116782 444792 353593 702168 485296 420 11686 596609 14514 3 4 3 43 26454 114080 413157 348773 678732 451967 446 12659 560848 15765 2 2 2 44 36909 135264 409919 334919 638193 448765 510 12374 556021 16965 2 2 2 45 26321 104658 373643 286950 560551 404697 388 11575 498745 12917 2 2 2 46 29835 109051 468118 336078 655869 484622 758 12060 595733 14409 3 4 3
DATA HASIL OPTIMASI HARIAN ICP-QMS (lanjutan) Analit
Noise pada m/z
Nomor analisis
Li Mg Rh Pb U Ba BaO Ba++ Ce CeO 5 220 260
47 36111 128309 471883 340264 667097 505871 601 13708 612433 17031 3 4 3 48 37546 129929 457551 353076 670072 502173 580 13958 600525 17183 5 4 5 49 37377 127476 461011 413153 766382 523161 506 12444 633478 15587 5 5 4 50 32403 112597 397945 354130 670842 458121 461 11290 555786 13386 4 3 3 51 43697 140474 477600 470493 881231 571097 624 14122 684577 20215 4 4 3 52 40763 141587 571949 535544 975671 656579 733 13983 786521 20879 4 5 5 53 36399 145092 595294 561443 1026240 690866 795 15291 825538 22919 4 4 3 54 48467 170758 615331 584534 1098235 722542 877 16238 866005 25610 3 4 5 55 34045 133950 576383 535323 1002518 665252 770 15494 802421 21877 4 3 4 56 25949 103081 554288 453631 873783 586410 569 10716 710204 15505 5 6 5 57 34468 134786 585910 497457 955088 665641 690 15162 795593 23654 3 5 3 58 39351 149965 604521 539311 1026360 685042 635 15596 821917 21564 4 4 4 59 37435 145172 524405 451034 856797 584787 622 15088 713076 20577 3 4 4 60 44461 157245 565539 498306 946160 624675 619 16069 750170 22191 3 4 4 61 47826 161618 496575 435596 830653 556027 589 13859 681018 19846 3 3 2 62 43214 150407 470139 401376 768876 519020 540 13414 634971 17719 3 3 2 63 40281 131169 470628 422288 810750 524030 557 13405 635131 19913 4 4 4 64 41176 149056 586494 522700 986340 649729 600 15563 777606 20383 4 4 5 65 41480 140946 605945 459777 852488 617125 507 15654 742833 19530 5 5 6 66 43882 148871 605289 473778 865710 629692 548 16521 751641 20990 5 5 5 67 29821 121850 600232 485373 874654 643480 586 15964 774912 20664 3 5 5 68 36578 135036 590164 489202 893584 653650 607 15981 784441 21746 4 4 6 69 33941 125815 590240 474253 860574 649343 673 16374 783594 22711 3 3 3 70 38966 122460 447903 369871 693643 496670 582 10513 601207 17430 3 4 4
DATA HASIL OPTIMASI HARIAN ICP-QMS (lanjutan) Analit
Noise pada m/z
Nomor analisis
Li Mg Rh Pb U Ba BaO Ba++ Ce CeO 5 220 260
71 24134 95717 563399 460478 842882 609250 613 12910 727754 18881 4 5 5 72 36796 136402 618853 539070 957461 694646 700 15102 829206 23341 4 4 4 73 48985 161717 691684 585732 1050733 765017 780 16981 909103 27396 4 4 5 74 37573 134827 628532 546349 995577 701766 652 13126 838790 21717 5 6 5 75 37526 133506 631394 555389 994954 703665 754 13242 835672 22972 4 6 5 76 30197 116832 629656 545560 986740 701068 676 13350 837473 21714 5 6 5 77 28555 115195 602938 525786 951362 673284 702 13166 806647 21744 4 5 4 78 35240 125047 596569 578435 1088827 640683 661 12398 769450 21317 6 6 6 79 28126 122500 519053 522391 979798 590417 668 11957 716388 21004 4 4 5 80 20862 95706 619448 585031 1104836 682854 649 13977 824818 21506 5 4 6 81 27168 115856 507023 475738 905233 555783 685 10946 680205 19969 4 5 3 82 31610 132037 586523 592589 1118631 675721 763 12804 814358 23856 4 5 5 83 43308 142769 458297 476049 897190 544996 637 11506 665707 20100 3 3 3 84 50261 156734 513764 555012 1043047 631334 710 13409 758314 25037 3 3 3 85 25538 109766 516681 507541 951260 589132 648 11334 715024 19932 4 5 4 86 31725 122493 477549 484606 911239 556272 619 11030 671658 19043 4 3 4 87 30758 119731 522193 507067 970944 597600 617 12366 723927 19239 4 4 3 88 28587 112422 421848 415788 784871 498139 591 11449 608466 18848 3 3 3 89 22417 86041 396370 361482 704369 460269 461 10422 557321 15277 4 4 3 90 28840 106013 416051 372735 712181 472288 480 9771 579168 15380 3 4 2 91 28222 117159 454141 362793 752795 487019 548 10839 584722 16673 3 4 5 92 30638 123634 484456 372534 771945 516890 551 12599 621701 17542 3 3 4 93 29569 124795 491306 383670 793503 528592 611 12789 635229 18757 3 3 3 94 28924 78273 341792 266497 551395 389269 441 6662 453312 14654 4 4 5
DATA HASIL OPTIMASI HARIAN ICP-QMS (lanjutan) Analit
Noise pada m/z
Nomor analisis
Li Mg Rh Pb U Ba BaO Ba++ Ce CeO 5 220 260
95 21452 55029 202029 135990 287039 221296 253 4913 266258 8570 1 1 1 96 45164 88906 342030 222252 477260 347429 355 6327 410800 12434 4 3 4 97 44508 95906 372627 241703 518627 378236 392 6952 441898 13321 4 4 4 98 41508 116014 501213 316754 673621 495130 459 9009 566376 15337 8 8 6 99 36016 102686 475275 373584 827758 514093 488 7323 590948 16739 6 7 7 100 28656 87705 406730 328641 709326 434810 370 7826 503924 12946 8 7 6 101 36260 102046 517477 416844 820137 604899 611 10436 687148 21909 5 5 6 102 37929 114008 560384 435227 815689 655035 604 10305 743281 20995 6 6 6 103 28085 98802 569303 402650 759525 644604 549 9945 732762 18169 7 7 6 104 21841 90965 465072 342603 980591 469002 474 7626 553258 16493 3 2 2 105 17514 78948 453271 320002 869028 472296 584 8556 548107 18356 2 2 2 106 18306 88087 471218 282064 726316 482528 3766 12037 571519 18073 3 2 3 107 22300 82605 384244 287892 799389 411314 445 7165 485679 16115 2 2 1 108 14898 68556 390516 290232 801680 427527 460 6315 507448 14239 1 1 1 109 12408 60623 382485 248665 653968 416272 415 6208 495768 13444 2 1 1 110 8358 42267 369078 282398 821212 376237 386 4837 453525 11703 4 4 3 111 9840 51767 392221 323913 920868 414539 419 5490 495560 15900 3 3 3 112 6655 26996 383598 326393 851320 447767 499 5598 533272 17600 2 3 3 113 9696 46257 453051 331735 893847 496026 519 7016 583854 19187 3 4 3 114 11345 53684 370608 323205 853841 486209 468 6505 590643 15960 2 3 3
LAMPIRAN G
HASIL ANALISIS DAN UJI STATISTIK MATERIAL REFERENS STANDAR
G.1. Hasil analisis
Tabel 1 Hasil analisis material referens standar Bush twigs.* Hasil analisis Unsur I II III Rerata SD % recovery Mg 0,269 0,297 0,288 0,285 0,014 99,19% Ca 2,019 2,216 2,281 2,172 0,136 97,84% Mn 59,682 61,385 53,497 58,188 4,151 100,32% Zn 22,628 20,057 20,786 21,157 1,325 102,70% Mo 0,2978 0,2865 0,2071 0,264 0,049 101,46% Cd 0,131 0,142 0,169 0,147 0,020 105,24%
*Satuan konsentrasi untuk Mg dan Ca dalam persen sedangkan unsur lain dalam µg/g. (berat sampel = ± 100 mg, faktor pengenceran = 30 x; n = 3). Persen recovery dihitung berdasarkan perbandingan hasil nilai analisis dengan nilai tersertifikasi (Tabel IV.2).
Tabel 2 Hasil analisis material referens standar Bush leaves.* Hasil analisis Unsur I II III Rerata SD % recovery Mg 0,472 0,527 0,408 0,469 0,060 97,71% Ca 1,538 1,749 1,72794 1,672 0,116 99,50% Mn 64,492 66,385 56,497 62,458 5,248 102,39% Zn 51,483 60,296 58,372 56,717 4,634 103,12% Mo 0,342 0,2284 0,291 0,287 0,057 102,55% Cd 0,412 0,427 0,409 0,416 0,010 109,47%
*Satuan konsentrasi untuk Mg dan Ca dalam persen sedangkan unsur lain dalam µg/g. (berat sampel = ± 100 mg, faktor pengenceran = 30 x; n = 3). Persen recovery dihitung berdasarkan perbandingan hasil nilai analisis dengan nilai tersertifikasi (Tabel IV.2).
Tabel 3 Hasil analisis material referens standar Poplar leaves.* Hasil analisis Unsur I II III Rerata SD % recovery Mg 0,7049 0,66956 0,7909 0,722 0,062 111,04% Ca 1,141 1,9851 1,744 1,623 0,435 89,69% Mn 50,728 43,414 51,156 48,433 4,352 107,63% Zn 43,988 31,165 47,296 40,816 8,520 110,31% Mo 0,2192 0,182 0,1498 0,184 0,035 102,04% Cd 0,391 0,316 0,377 0,361 0,040 112,92%
*Satuan konsentrasi untuk Mg dan Ca dalam persen sedangkan unsur lain dalam µg/g. (berat sampel = ± 100 mg, faktor pengenceran = 30 x; n = 3). Persen recovery dihitung berdasarkan perbandingan hasil nilai analisis dengan nilai tersertifikasi (Tabel IV.2).
G.2. Hasil uji statistik
G.2.1. T-Test kandungan Mg dalam bush twigs
One-Sample Statistics
3 .284667 .0142945 .0082529 Kandungan Mg
dalam bush twigs
N Mean Std. Deviation Std. Error Mean One-Sample Test -.283 2 .804 -.002333 -.037843 .033176 Kandungan Mg dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.287
G.2.2. T-Test kandungan Ca dalam bush twigs
One-Sample Statistics
3 2.172000 .1364295 .0787676 Kandungan Ca
dalam bush twigs
N Mean Std. Deviation Std. Error Mean One-Sample Test -.609 2 .604 -.048000 -.386910 .290910 Kandungan Ca dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 2.220
G.2.3. T-Test kandungan Mn dalam bush twigs
One-Sample Statistics
3 58.188000 4.1508027 2.3964671 Kandungan Mn
dalam bush twigs
N Mean Std. Deviation
Std. Error Mean
One-Sample Test
.078 2 .945 .188000 -10.1232 10.499166
Kandungan Mn dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 58.000
G.2.4. T-Test kandungan Zn dalam bush twigs
One-Sample Statistics
3 21.157000 1.3250438 .7650144 Kandungan Zn
dalam bush twigs
N Mean Std. Deviation Std. Error Mean One-Sample Test .728 2 .542 .557000 -2.734591 3.848591 Kandungan Zn dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 20.600
G.2.5. T-Test kandungan Mo dalam bush twigs
One-Sample Statistics
3 .263800 .0494276 .0285371 Kandungan Mo
dalam bush twigs
N Mean Std. Deviation Std. Error Mean One-Sample Test .133 2 .906 .003800 -.118985 .126585 Kandungan Mo dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.260
G.2.6. T-Test kandungan Cd dalam bush twigs
One-Sample Statistics
One-Sample Test
.650 2 .583 .007333 -.041240 .055907
Kandungan Cd dalam bush twigs
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.140
G.2.7. T-Test kandungan Mg dalam bush leaves
One-Sample Statistics
3 .469000 .0595567 .0343851 Kandungan Mg
dalam bush leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test -.320 2 .779 -.011000 -.158947 .136947 Kandungan Mg dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.480
G.2.8. T-Test kandungan Ca dalam bush leaves
One-Sample Statistics
3 1.671647 .1162194 .0670993 Kandungan Ca
dalam bush leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test -.124 2 .912 -.008353 -.297058 .280352 Kandungan Ca dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 1.680
G.2.9. T-Test kandungan Mn dalam bush leaves
One-Sample Statistics
N Mean Std. Deviation
Std. Error Mean
One-Sample Test
.481 2 .678 1.458000 -11.5798 14.495819
Kandungan Mn dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 61.000
G.2.10. T-Test kandungan Zn dalam bush leaves
One-Sample Statistics
3 56.717000 4.6337362 2.6752888 Kandungan Zn
dalam bush leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test .642 2 .587 1.717000 -9.793839 13.227839 Kandungan Zn
dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 55.000
G.2.11. T-Test kandungan Mo dalam bush leaves
One-Sample Statistics
3 .287133 .0568986 .0328504 Kandungan Mo
dalam bush leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test .217 2 .848 .007133 -.134211 .148477 Kandungan Mo dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.280
G.2.12. T-Test kandungan Cd dalam bush leaves
One-Sample Statistics
One-Sample Test
6.466 2 .023 .036000 .012044 .059956
Kandungan Cd dalam bush leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.380
G.2.13. T-Test kandungan Mg dalam poplar leaves
One-Sample Statistics 3 .721787 .0624077 .0360311 MGPL N Mean Std. Deviation Std. Error Mean One-Sample Test 1.992 2 .185 .071787 -.083243 .226816 Kandungan Mg dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.650
G.2.14. T-Test kandungan Ca dalam poplar leaves
One-Sample Statistics
3 1.623367 .4347879 .2510249 Kandungan Ca
dalam poplar leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test -.743 2 .535 -.186633 -1.266706 .893440 Kandungan Ca dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 1.810
G.2.15. T-Test kandungan Mn dalam poplar leaves
One-Sample Statistics
Kandungan Mn
N Mean Std. Deviation
Std. Error Mean
One-Sample Test
1.366 2 .305 3.432667 -7.377203 14.242536
Kandungan Mn dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 45.000
G.2.16. T-Test kandungan Zn dalam poplar leaves
One-Sample Statistics
3 40.816333 8.5203810 4.9192443 Kandungan Zn
dalam poplar leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test .776 2 .519 3.816333 -17.3495 24.982133 Kandungan Zn dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 37.000
G.2.17. T-Test kandungan Mo dalam poplar leaves
One-Sample Statistics
3 .183667 .0347300 .0200514 Kandungan Mo
dalam poplar leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test .183 2 .872 .003667 -.082607 .089941 Kandungan Mo dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.180
G.2.18. T-Test kandungan Cd dalam poplar leaves
One-Sample Statistics
3 .361333 .0398790 .0230241 Kandungan Cd
dalam poplar leaves
N Mean Std. Deviation Std. Error Mean One-Sample Test 1.795 2 .214 .041333 -.057732 .140398 Kandungan Cd dalam poplar leaves
t df Sig. (2-tailed)
Mean
Difference Lower Upper 95% Confidence
Interval of the Difference Test Value = 0.320
LAMPIRAN H
KURVA KALIBRASI BERAT MOLEKUL RELATIF KOLOM SEPHADEX G-50
G.1. Kurva kalibrasi
Kurva kalibrasi berat molekul pemisahan SEC pada kolom sephadex G-50 ditentukan dengan membuat kurva hubungan antara log BM (berat molekul) dan nilai Kav, yaitu angka banding selisih volume elusi dan volume mati dengan selisih volume total kolom dan volume mati. Kav dihitung berdasarkan persamaan:
) ( ) ( Vo Vt Vo Ve Kav − − =
Dimana, Ve = Volume elusi; Vt = volume total kolom; dan Vo = volume mati. Vt = 3,14 x (1,2cm)2 x 70 cm = 317 cm3 (mL) Vo = 96 cm Kav = - 0,5189 log BM + 2,4585 R2 = 0,9998 0 0,3 0,6 0,9 3,0 3,5 4,0 4,5 5,0
Log berat molekul
Kav
Myoglobin Vit. B12
Ovalbumin
Tabel F1. Data perhitungan kurva kalibrasi BM relatif pada kolom sephadex G-50 Protein Standar Berat Molekul (BM, Da) Log BM No. Fraksi Volume elusi (Ve, mL) Kav thyroglobulin 670000 Vo Bovin globulin 158000 Vo Chicken ovalbumin 44000 4,643 15 108,0 0,054 Equine myoglobin 17500 4,243 21 151,2 0,250 Vitamin B12 1350 3,130 39 280,8 0,836
G.2. Perhitungan BM relatif spesi
Tabel F2. Data perhitungan BM relatif spesi Mg, Ca, Mn, Zn, Mo, dan Cd pada kolom sephadex G-50 (buffer 20 mM MES/ 1 mM NaN3 pH 8,0).
Spesi Volume elusi (Ve, mL) Kav Log BM BM relatif (Da) MgA1 Vo > 44.000a) MgA2 266,4 0,771 3,250 1780 ± 250 MgA3 309,6 0,967 2,874 < 1350b) CaA1 Vo > 44.000 Ca A2 295,2 0,901 2,999 < 1350 Ca A3 331,2 1,064 2,685 < 1350 MnA1 Vo > 44.000 MnA2 295,2 0,901 2,999 < 1350 MnA3 331,2 1,064 2,685 < 1350 ZnA1 Vo > 44.000 ZnA2 259,2 0,738 3,313 2057 ± 300 ZnA3 280,8 0,836 3,130 1350 ± 200 MoA1 Vo > 44.000 MoA2 316,8 0,999 2,811 < 1350 CdA1 Vo > 44.000 CdA2 158,4 0,282 4,192 15560 ± 2000 CdA3 252,0 0,706 3,376 2377 ± 300 CdA4 288,0 0,869 3,062 < 1350 CdA5 309,6 0,967 2,874 < 1350 a)
Spesi yang terelusi pada Vo mempunyai BM relatif > 44 kDa.
b)
LAMPIRAN I
DATA CACAH PERDETIK NETTO C, P, DAN S I.1 Data cacah perdetik C, P, dan S pada kolom sephadex G-25
Cacah perdetik (cps) netto
(hasil bagi cps analit dengan cps In sebagai standar internal)
Hasil pengukuran Data pada kurva profil elusi C,P,S Volume elusi (mL) C P S C P S Blanko 0,021 0,018 0,649 0,1 0,021 0,017 0,649 0,000 0,000 0,000 0,2 0,021 0,018 0,661 0,000 0,000 0,000 0,3 0,021 0,018 0,659 0,000 0,000 0,000 0,4 0,021 0,017 0,649 0,000 0,000 0,000 0,5 0,021 0,017 0,659 0,000 0,000 0,238 0,6 0,021 0,017 0,649 0,000 0,000 0,000 0,7 0,022 0,018 0,645 0,091 0,000 0,000 0,8 0,023 0,027 0,675 0,182 0,009 0,618 0,9 0,025 0,073 0,663 0,364 0,055 0,333 1,0 0,023 0,209 0,541 0,182 0,191 0,000 1,1 0,025 0,322 0,666 0,364 0,304 0,404 1,2 0,027 0,432 0,686 0,545 0,414 0,879 1,3 0,034 0,603 0,702 1,182 0,585 1,259 1,4 0,050 0,882 0,704 2,636 0,864 1,306 1,5 0,081 1,312 0,736 5,455 1,294 2,067 1,6 0,131 1,879 0,758 10,000 1,861 2,589 1,7 0,167 2,352 0,786 13,273 2,334 3,254 1,8 0,226 2,220 0,829 18,636 2,202 4,276 1,9 0,245 2,466 0,810 20,364 2,448 3,824 2,0 0,233 2,511 0,785 19,273 2,493 3,230 2,1 0,208 2,422 0,763 17,000 2,404 2,708 2,2 0,159 2,708 0,702 12,545 2,690 1,259 2,3 0,115 2,018 0,682 8,545 2,000 0,793 2,4 0,076 1,149 0,633 5,000 1,131 0,000 2,5 0,050 0,650 0,618 2,636 0,632 0,000 2,6 0,038 0,394 0,624 1,545 0,376 0,000 2,7 0,029 0,225 0,623 0,727 0,207 0,000 2,8 0,025 0,130 0,626 0,364 0,112 0,000 2,9 0,024 0,098 0,621 0,273 0,080 0,000 3,0 0,018 0,049 0,494 0,000 0,031 0,000 3,1 0,021 0,048 0,636 0,000 0,030 0,000 3,2 0,021 0,040 0,628 0,000 0,022 0,000 3,3 0,021 0,032 0,625 0,000 0,014 0,000 3,4 0,022 0,031 0,647 0,091 0,013 0,000 3,5 0,021 0,026 0,650 0,000 0,008 0,024 3,6 0,020 0,023 0,643 0,000 0,005 0,000 3,7 0,021 0,024 0,660 0,000 0,006 0,261 3,8 0,020 0,024 0,657 0,000 0,006 0,190 3,9 0,021 0,024 0,654 0,000 0,006 0,119 4,0 0,022 0,021 0,666 0,091 0,003 0,404
I.1 Data cacah perdetik C, P, dan S pada kolom sephadex G-50 Cacah perdetik netto
Hasil pengukuran Data pada kurva profil elusi C,P,S Volume elusi (mL) C P S C P S Blanko 0,051 0,066 1,149 7,2 0,036 0,062 1,172 0,000 0,000 0,056 14,4 0,035 0,071 1,192 0,000 0,001 0,106 21,6 0,035 0,068 1,186 0,000 0,000 0,091 28,8 0,048 0,067 1,199 0,000 0,000 0,124 36,0 0,035 0,067 1,121 0,000 0,000 0,000 43,2 0,035 0,066 1,122 0,000 0,000 0,000 50,4 0,035 0,068 1,122 0,000 0,000 0,000 57,6 0,032 0,065 1,183 0,000 0,000 0,084 64,8 0,039 0,067 1,192 0,000 0,000 0,106 72,0 0,039 0,064 1,193 0,000 0,000 0,109 79,2 0,056 0,062 1,189 0,045 0,000 0,099 86,4 0,058 0,065 1,196 0,064 0,000 0,116 93,6 0,068 0,073 1,216 0,155 0,001 0,166 100,8 0,064 0,077 1,196 0,118 0,002 0,116 108,0 0,054 0,072 1,184 0,027 0,001 0,086 115,2 0,039 0,065 1,212 0,000 0,000 0,156 122,4 0,036 0,067 1,202 0,000 0,000 0,131 129,6 0,047 0,069 1,212 0,000 0,001 0,156 136,8 0,046 0,066 1,181 0,000 0,000 0,079 144,0 0,045 0,068 1,184 0,000 0,000 0,086 151,2 0,047 0,063 1,192 0,000 0,000 0,106 158,4 0,046 0,067 1,212 0,000 0,000 0,156 165,6 0,046 0,064 1,200 0,000 0,000 0,126 172,8 0,046 0,067 1,206 0,000 0,000 0,141 180,0 0,046 0,069 1,207 0,000 0,001 0,143
I.1 Data cacah perdetik C, P, dan S pada kolom sephadex G-50 (lanjutan) Cacah perdetik netto
Hasil pengukuran Data pada kurva profil elusi C,P,S Volume elusi (mL) C P S C P S 187,2 0,045 0,066 1,225 0,000 0,000 0,188 194,4 0,044 0,067 1,195 0,000 0,000 0,114 201,6 0,045 0,070 1,172 0,000 0,001 0,056 208,8 0,044 0,215 1,197 0,000 0,030 0,119 216,0 0,045 0,486 1,227 0,000 0,084 0,193 223,2 0,043 0,485 1,195 0,000 0,084 0,114 230,4 0,044 0,259 1,220 0,000 0,039 0,176 237,6 0,044 0,200 1,192 0,000 0,027 0,106 244,8 0,045 0,113 1,213 0,000 0,009 0,158 252,0 0,045 0,150 1,212 0,000 0,017 0,156 259,2 0,037 0,216 1,226 0,000 0,030 0,191 266,4 0,047 0,868 1,223 0,000 0,160 0,183 273,6 0,133 2,618 1,301 0,745 0,510 0,377 280,8 0,897 3,011 1,541 7,691 0,589 0,974 288,0 0,976 2,160 1,574 8,409 0,419 1,056 295,2 0,241 2,491 1,438 1,727 0,485 0,718 302,4 0,104 3,139 1,310 0,482 0,615 0,400 309,6 0,059 3,063 1,226 0,073 0,599 0,191 316,8 0,040 1,413 1,174 0,000 0,269 0,061 324,0 0,037 0,369 1,174 0,000 0,061 0,061 331,2 0,036 0,128 1,203 0,000 0,012 0,133 338,4 0,037 0,078 1,189 0,000 0,002 0,099 345,6 0,048 0,098 1,183 0,000 0,006 0,084 352,8 0,035 0,080 1,389 0,000 0,003 0,596 360,0 0,035 0,070 1,200 0,000 0,001 0,126 367,2 0,035 0,066 1,175 0,000 0,000 0,064
LAMPIRAN J
PENGOLAHAN DATA PEMISAHAN DENGAN SEC
J.1 Contoh data mentah hasil analisis ICP MS
Date : Thursday April 08 2004 23:34:26
Sample: Fract.2
c:\userdata2\Sample\NoorFitri\030415XylemundPhloemsaft.sam c:\userdata2\Method\NoorFitri\Quant\040406 NF Quant ext.kalib. for Phloem.mth
c:\userdata2\Dataset\noorfitri\phloem of ricinus communis\ab umzug\usn\Fract.2.070
Analit Massa Konsentrasi (µg/L) SD Rerata cps Rerata cps netto* RSD cps netto B 11 7,66536 0,16567 6064 0,01202 2,16 C 13 582758 1,15483 1,61 N 15 29901 0,05926 4,11 Mg 25 2,15578 0,03404 2052 0,00407 1,58 P 31 18646 0,03696 4,58 S 34 67176 0,13312 0,77 Cl 35 208398 0,41298 1,57 Ca 43 54,06192 6,28847 1884 0,00373 11,63 Ca 44 119,88395 4,53749 67190 0,13316 3,78 Mn 55 0,11043 0,00572 2937 0,00582 5,18 Zn 66 10,55074 0,20457 23703 0,04697 1,94 Zn 68 3,81135 0,03707 5961 0,01181 0,97 Mo 95 0,04487 0,00239 291 0,00058 5,33 Mo 97 0,04077 0,00359 170 0,00034 8,81 Cd 112 0,00904 0,00152 80 0,00016 16,86 Cd 114 0,00807 0,00099 88 0,00017 12,27 In 115 504644 0,78
*Rerata cps netto adalah hasil bagi cps analit dengan cps In sebagai standar internal. Pada larutan standar untuk kalibrasi Cps analit dalam larutan standar dikoreksi dengan cps blanko. Data rerata cps netto yang dikonversi menjadi konsentrasi melalui kurva kalibrasi.
J.2 Pemisahan pada kolom sephadex G-50
J.2.1 Pemisahan Sampel 500 µL cairan floem dengan kultur Aeroponik dalam fasa gerak: 20 mM MES/1 mM NaN3 pH 8,0.
Tabel J. 1. Deret larutan standar untuk pembuatan kurva kalibrasi
larutan Mg Ca Mn Zn Mo Cd Blanko 0,000 0,000 0,000 0,000 0,000 0,000 Std 1 0,102 9,990 0,103 1,040 0,010 0,010 Std 2 1,020 49,950 0,515 5,200 0,102 0,102 Std 3 10,200 99,900 1,030 10,400 0,510 0,510 Std 4 51,000 499,500 5,150 52,000 1,020 1,020 Std 5 102,000 999,000 10,300 104,000 10,200 10,200
Tabel J. 2. Rerata cps larutan standar
larutan Mg Ca Mn Zn Mo Cd In Blanko 531 190 2385 6668 375 45 559374 Std 1 631 445 5438 8177 452 158 555231 Std 2 1139 2162 17032 14282 1094 1170 560755 Std 3 11308 4085 32534 22079 4081 5681 551461 Std 4 56916 20231 152374 84174 7716 11265 554381 Std 5 113846 40524 306527 163174 73857 115441 559119
Tabel J. 3. Rerata cps netto larutan standar
larutan Mg Ca Mn Zn Mo Cd Std 1 0,00018 0,000459 0,00550 0,00272 0,00014 0,00020 Std 2 0,00108 0,003517 0,02612 0,01358 0,00128 0,00201 Std 3 0,01954 0,007063 0,05467 0,02795 0,00672 0,01022 Std 4 0,10171 0,03615 0,27055 0,13981 0,01324 0,02024 Std 5 0,20267 0,072139 0,54397 0,27992 0,13142 0,20639
Tabel J. 4. Perhitungan persamaan regressi dan R2*
Isotop Persamaan regressi R2
Mg y = 0,001994x - 0,000493 0,999973 Ca y = 0,000072x - 0,000158 0,999991 Mn y = 0,052810x - 0,000426 0,999989 Zn y = 0,002693x - 0,000200 0,999998 Mo y = 0,012880x + 0,000056 0,999998 Cd y = 0,020245x - 0,000137 0,999997
Tabel J. 5. Konsentrasi larutan blanko larutan Mg Ca Mn Zn Mo Cd Blanko A1 0,11722 43,24020 0,03693 1,05634 0,00519 0,00289 Blanko A2 0,12704 43,70070 0,03617 0,99401 0,00437 0,00290 Blanko A3 0,11155 43,53572 0,03557 1,01274 0,00449 0,00277 Rerata 0,11860 43,49221 0,03622 1,02103 0,00468 0,00285 SD 0,00784 0,23331 0,00068 0,03198 0,00044 0,00007 RSD 6,61% 0,54% 1,88% 3,13% 9,46% 2,54% Blanko B1 0,46731 92,72965 0,07847 4,06093 0,05348 0,00385 Blanko B2 0,47482 97,49874 0,08110 3,97676 0,05276 0,00406 Blanko B3 0,48060 98,41443 0,07775 3,93166 0,05267 0,00332 Rerata 0,47424 96,21427 0,07911 3,98978 0,05297 0,00374 SD 0,00666 3,05231 0,00176 0,06561 0,00044 0,00038 RSD 1,41% 3,17% 2,23% 1,64% 0,84% 10,19% Blanko C1 0,56211 95,42761 0,11668 0,82789 0,05761 0,00342 Blanko C2 0,52546 97,09210 0,12525 0,82103 0,06049 0,00324 Blanko C3 0,53998 96,62562 0,12451 0,84932 0,06003 0,00277 Rerata 0,54252 96,38178 0,12215 0,83275 0,05938 0,00314 SD 0,01846 0,85862 0,00475 0,01476 0,00155 0,00034 RSD 3,40% 0,89% 3,89% 1,77% 2,61% 10,68% B.Deteksi 0,59789 98,95763 0,13639 0,87702 0,06402 0,00415 B.Kuantifi kasi (BK) 0,72708 104,96797 0,16963 0,98032 0,07485 0,00650 Blanko terdiri dari In 10 µg/L dalam larutan HNO3 1%. Blanko A dalam larutan air, blanko B dalam fasa gerak yang digunakan. Blanko C dalam fasa gerak yang telah melewati kolom. Batas deteksi = rerata + 3 x SD dan batas kuantifikasi = rerata + 10 x SD.
Konsentrasi akhir spesi (Ca) yang diplot dalam kurva profil elusi dihitung
berdasarkan persamaan berikut:
Ca = (C0 – BK) x fp; jika Ca < 0, maka Ca = 0
Dimana, C0 adalah konsentrasi spesi yang terukur oleh alat (Tabel G.6);
BK adalah batas kuantifikasi (Tabel G.5.) dan fp adalah faktor pengenceran (fp = 4/3,6 (1,111); pada kasus tertentu bisa 30 kali).
Tabel J. 6. Konsentrasi spesi (C0) dalam larutan fraksi yang diukur No. Fr. Ve (mL) Mg Ca Mn Zn Mo Cd Fr.1 7,2 2,148 102,738 0,109 4,294 0,051 0,009 Fr.2 14,4 2,156 101,884 0,110 3,811 0,045 0,008 Fr.3 21,6 1,949 100,964 0,114 4,446 0,050 0,007 Fr.4 28,8 2,081 103,284 0,118 4,226 0,048 0,008 Fr.5 36,0 1,845 102,900 0,104 4,441 0,046 0,007 Fr.6 43,2 1,840 103,575 0,103 4,486 0,047 0,007 Fr.7 50,4 1,964 101,940 0,112 4,979 0,049 0,008 Fr.8 57,6 1,828 102,674 0,106 4,559 0,048 0,008 Fr.9 64,8 1,830 101,181 0,102 4,841 0,046 0,008 Fr.10 72,0 1,850 102,279 0,104 4,543 0,047 0,007 Fr.11 79,2 1,883 102,708 0,101 4,551 0,046 0,007 Fr.12 86,4 1,973 110,053 0,107 4,953 0,067 0,008 Fr.13 93,6 2,014 113,784 0,127 9,700 0,358 0,016 Fr.14 100,8 2,068 116,923 0,132 11,047 0,350 0,029 Fr.15 108,0 1,957 109,733 0,121 5,787 0,092 0,015 Fr.16 115,2 1,928 109,073 0,117 6,378 0,062 0,014 Fr.17 122,4 1,876 108,464 0,105 4,765 0,059 0,012 Fr.18 129,6 1,890 108,339 0,106 4,952 0,053 0,011 Fr.19 136,8 1,901 105,620 0,107 5,422 0,053 0,011 Fr.20 144,0 1,944 108,450 0,107 5,581 0,054 0,011 Fr.21 151,2 1,860 103,952 0,103 5,050 0,052 0,015 Fr.22 158,4 1,938 107,292 0,105 5,257 0,056 0,035 Fr.23 165,6 1,960 107,244 0,112 4,872 0,050 0,038 Fr.24 172,8 1,933 107,470 0,106 4,611 0,050 0,021 Fr.25 180,0 1,904 103,439 0,102 4,756 0,049 0,013 Fr.26 187,2 1,965 102,562 0,102 5,611 0,050 0,011 Fr.27 194,4 1,934 108,222 0,104 4,489 0,048 0,010 Fr.28 201,6 1,944 105,779 0,103 4,505 0,051 0,010 Fr.29 208,8 1,903 103,536 0,103 4,414 0,051 0,010 Fr.30 216,0 1,940 106,901 0,104 4,695 0,051 0,011 Fr.31 223,2 1,966 104,779 0,108 5,417 0,051 0,014 Fr.32 230,4 2,054 104,480 0,110 7,210 0,053 0,018 Fr.33 237,6 1,971 103,876 0,113 7,778 0,050 0,023 Fr.34 244,8 1,970 106,208 0,117 9,468 0,052 0,043 Fr.35 252,0 5,988 103,809 0,127 11,123 0,052 0,044 Fr.36 259,2 381,854 110,266 0,401 13,212 0,054 0,039 Fr.37 266,4 1843,357 111,252 1,738 8,176 0,053 0,023 Fr.38 273,6 1683,506 108,974 2,421 20,760 0,055 0,013 Fr.39 280,8 1108,172 125,564 3,972 144,161 0,064 0,015 Fr.40 288,0 837,779 247,719 5,605 122,597 0,089 0,021 Fr.41 295,2 329,806 609,337 6,188 34,199 0,175 0,010 Fr.42 302,4 105,715 464,368 3,597 6,649 1,843 0,010 Fr.43 309,6 40,023 234,686 0,791 2,105 11,609 0,031 Fr.44 316,8 32,093 162,343 0,563 1,392 12,087 0,030 Fr.45 324,0 49,470 179,201 0,907 1,418 3,038 0,011
Tabel J.6. Konsentrasi spesi (C0) dalam larutan fraksi yang diukur (lanjutan). No. Fr. Ve (mL) Mg Ca Mn Zn Mo Cd Fr.48 345,6 1,548 129,958 0,775 1,091 0,083 0,004 Fr.49 352,8 1,139 114,921 0,528 1,217 0,082 0,004 Fr.50 360,0 1,028 106,084 0,423 6,550 0,075 0,004 Fr.51 367,2 0,823 103,120 0,403 1,531 0,072 0,004 Fr.52 374,4 0,850 103,041 0,400 1,004 0,068 0,003 Fr.53 381,6 0,729 97,768 0,385 0,882 0,068 0,003 Fr.54 388,8 0,737 99,843 0,382 0,916 0,071 0,004 Fr.55 396,0 0,771 99,346 0,384 2,323 0,065 0,003 Fr.56 403,2 0,705 93,090 0,362 1,447 0,067 0,003 Fr.57 410,4 0,674 94,207 0,349 0,890 0,066 0,003 Fr.58 417,6 0,660 91,811 0,334 0,781 0,064 0,004 Fr.59 424,8 0,675 95,951 0,326 0,910 0,066 0,003 Fr.60 432,0 0,630 89,938 0,308 0,848 0,066 0,003 Fr.61 439,2 0,611 94,933 0,305 0,748 0,062 0,003 Fr.62 446,4 0,611 93,410 0,294 0,796 0,064 0,003 Fr.63 453,6 0,617 91,268 0,280 0,776 0,064 0,003 Fr.64 460,8 0,614 98,428 0,272 0,787 0,067 0,004 Fr.65 468,0 0,610 92,747 0,263 0,851 0,065 0,004 Fr.66 475,2 0,677 90,273 0,256 2,639 0,062 0,003 Fr.67 482,4 0,655 93,214 0,248 0,836 0,063 0,004 Fr.68 489,6 0,729 96,359 0,248 1,066 0,063 0,003 Fr.69 496,8 0,559 95,777 0,233 0,783 0,063 0,002 Fr.70 504,0 0,598 96,293 0,221 0,849 0,064 0,004 Fr.71 511,2 0,687 95,571 0,248 0,467 0,046 0,004 Fr.72 518,4 0,773 98,264 0,130 0,984 0,069 0,003 Fr.73 525,6 0,788 95,693 0,106 0,285 0,055 0,002 Fr.74 532,8 0,860 98,477 0,127 0,485 0,057 0,002 Fr.75 540,0 0,554 95,403 0,171 0,714 0,066 0,003 Fr.76 547,2 0,442 95,306 0,159 0,241 0,052 0,002 Fr.77 554,4 0,555 98,192 0,132 0,899 0,067 0,002 Fr.78 561,6 0,927 98,539 0,111 0,176 0,064 0,003 Fr.79 568,8 0,585 92,216 0,181 0,531 0,064 0,005 Fr.80 576,0 0,176 91,569 0,143 0,847 0,063 0,003 Fr.81 583,2 0,038 90,128 0,140 0,590 0,065 0,002 Fr.82 590,4 0,953 96,088 0,116 0,454 0,067 0,003 Fr.83 597,6 0,349 94,45 0,135 0,446 0,064 0,003 Fr.84 604,8 0,670 98,416 0,120 0,920 0,054 0,003 Fr.85 612,0 0,465 98,206 1,135 0,917 0,068 0,003 Fr.86 619,2 0,786 96,032 0,108 0,065 0,062 0,003 Fr.87 626,4 0,043 90,436 0,130 0,825 0,059 0,003 Fr.88 633,6 0,456 97,402 0,122 0,428 0,065 0,002 Fr.89 640,8 0,439 97,359 0,148 0,296 0,065 0,002 Fr.90 648,0 0,065 99,105 0,110 0,249 0,063 0,003 Fr.91 655,2 0,690 95,929 0,132 0,580 0,068 0,002 Fr.92 662,4 0,737 95,903 0,153 0,386 0,064 0,002 Fr.93 669,6 0,261 99,224 0,148 0,184 0,058 0,003 Fr.94 676,8 0,392 99,507 0,150 0,999 0,064 0,003
Tabel J. 7. Konsentrasi spesi (Ca) yang diplot dalam kurva profil elusi. No. Fr. Ve (mL) Mg Ca Mn Zn Mo Cd Fr.1 7,2 1,579 0,000 0,000 3,682 0,000 0,003 Fr.2 14,4 1,587 0,000 0,000 3,146 0,000 0,002 Fr.3 21,6 1,358 0,000 0,000 3,851 0,000 0,001 Fr.4 28,8 1,504 0,000 0,000 3,607 0,000 0,001 Fr.5 36,0 1,242 0,000 0,000 3,845 0,000 0,001 Fr.6 43,2 1,237 0,000 0,000 3,895 0,000 0,001 Fr.7 50,4 1,374 0,000 0,000 4,443 0,000 0,002 Fr.8 57,6 1,223 0,000 0,000 3,976 0,000 0,002 Fr.9 64,8 1,225 0,000 0,000 4,289 0,000 0,001 Fr.10 72,0 1,248 0,000 0,000 3,958 0,000 0,001 Fr.11 79,2 1,285 0,000 0,000 3,968 0,000 0,001 Fr.12 86,4* 1,384 5,650 0,000 4,414 0,000 0,001 Fr.13 93,6 1,430 9,795 0,000 9,688 0,315 0,010 Fr.14 100,8 1,490 13,283 0,000 11,185 0,306 0,024 Fr.15 108,0 1,367 5,295 0,000 5,341 0,019 0,010 Fr.16 115,2 1,334 4,561 0,000 5,998 0,000 0,008 Fr.17 122,4 1,277 3,885 0,000 4,205 0,000 0,007 Fr.18 129,6 1,293 3,745 0,000 4,413 0,000 0,004 Fr.19 136,8 1,305 0,725 0,000 4,935 0,000 0,005 Fr.20 144,0 1,352 3,869 0,000 5,112 0,000 0,005 Fr.21 151,2 1,259 0,000 0,000 4,522 0,000 0,009 Fr.22 158,4 1,345 2,583 0,000 4,751 0,000 0,032 Fr.23 165,6 1,370 2,529 0,000 4,324 0,000 0,035 Fr.24 172,8 1,340 2,780 0,000 4,035 0,000 0,016 Fr.25 180,0 1,307 0,000 0,000 4,195 0,000 0,007 Fr.26 187,2 1,375 0,000 0,000 5,145 0,000 0,005 Fr.27 194,4 1,342 3,615 0,000 3,898 0,000 0,004 Fr.28 201,6 1,352 0,901 0,000 3,916 0,000 0,004 Fr.29 208,8 1,307 0,000 0,000 3,816 0,000 0,004 Fr.30 216,0 1,348 2,148 0,000 4,128 0,000 0,005 Fr.31 223,2 1,376 0,000 0,000 4,929 0,000 0,009 Fr.32 230,4 1,474 0,000 0,000 6,922 0,000 0,012 Fr.33 237,6 1,382 0,000 0,000 7,553 0,000 0,018 Fr.34 244,8 1,381 1,378 0,000 9,431 0,000 0,041 Fr.35 252,0 5,845 0,000 0,000 11,269 0,000 0,042 Fr.36 259,2 423,474 5,887 0,257 13,591 0,000 0,036 Fr.37 266,4 2047,366 6,983 1,743 7,995 0,000 0,018 Fr.38 273,6 1869,755 4,451 2,502 21,977 0,000 0,008 Fr.39 280,8 1230,494 22,884 4,225 159,089 0,000 0,010 Fr.40 288,0 930,058 158,613 6,039 135,130 0,016 0,016 Fr.41 295,2 365,643 560,410 6,687 36,909 0,112 0,004 Fr.42 302,4 116,654 399,333 3,809 6,299 1,965 0,004 Fr.43 309,6 43,662 144,131 0,690 1,250 12,816 0,027 Fr.44 316,8 34,850 63,750 0,437 0,458 13,347 0,026 Fr.45 324,0 54,159 82,481 0,819 0,486 3,293 0,005 Fr.46 331,2 28,650 193,444 2,290 0,531 0,313 0,000
Tabel J. 7. Konsentrasi spesi yang diplot dalam kurva profil elusi (lanjutan). No. Fr. Ve (mL) Mg Ca Mn Zn Mo Cd Fr.48 345,6 0,913 27,766 0,673 0,124 0,009 0,000 Fr.49 352,8 0,458 11,058 0,398 0,263 0,008 0,000 Fr.50 360,0 0,335 1,240 0,282 6,189 0,000 0,000 Fr.51 367,2 0,107 0,000 0,259 0,612 0,000 0,000 Fr.52 374,4 0,136 0,000 0,256 0,026 0,000 0,000 Fr.53 381,6 0,002 0,000 0,240 0,000 0,000 0,000 Fr.54 388,8 0,011 0,000 0,236 0,000 0,000 0,000 Fr.55 396,0 0,048 0,000 0,238 1,492 0,000 0,000 Fr.56 403,2 0,000 0,000 0,214 0,518 0,000 0,000 Fr.57 410,4 0,000 0,000 0,200 0,000 0,000 0,000 Fr.58 417,6 0,000 0,000 0,183 0,000 0,000 0,000 Fr.59 424,8 0,000 0,000 0,173 0,000 0,000 0,000 Fr.60 432,0 0,000 0,000 0,154 0,000 0,000 0,000 Fr.61 439,2 0,000 0,000 0,150 0,000 0,000 0,000 Fr.62 446,4 0,000 0,000 0,138 0,000 0,000 0,000 Fr.63 453,6 0,000 0,000 0,123 0,000 0,000 0,000 Fr.64 460,8 0,000 0,000 0,114 0,000 0,000 0,000 Fr.65 468,0 0,000 0,000 0,104 0,000 0,000 0,000 Fr.66 475,2 0,000 0,000 0,096 1,842 0,000 0,000 Fr.67 482,4 0,000 0,000 0,087 0,000 0,000 0,000 Fr.68 489,6 0,002 0,000 0,087 0,096 0,000 0,000 Fr.69 496,8 0,000 0,000 0,070 0,000 0,000 0,000 Fr.70 504,0 0,000 0,000 0,057 0,000 0,000 0,000 Fr.71 511,2 0,000 0,000 0,087 0,000 0,000 0,000 Fr.72 518,4 0,051 0,000 0,000 0,004 0,000 0,000 Fr.73 525,6 0,068 0,000 0,000 0,000 0,000 0,000 Fr.74 532,8 0,148 0,000 0,000 0,000 0,000 0,000 Fr.75 540,0 0,000 0,000 0,002 0,000 0,000 0,000 Fr.76 547,2 0,000 0,000 0,000 0,000 0,000 0,000 Fr.77 554,4 0,000 0,000 0,000 0,000 0,000 0,000 Fr.78 561,6 0,222 0,000 0,000 0,000 0,000 0,000 Fr.79 568,8 0,000 0,000 0,013 0,000 0,000 0,000 Fr.80 576,0 0,000 0,000 0,000 0,000 0,000 0,000 Fr.81 583,2 0,000 0,000 0,000 0,000 0,000 0,000 Fr.82 590,4 0,251 0,000 0,000 0,000 0,000 0,000 Fr.83 597,6 0,000 0,000 0,000 0,000 0,000 0,000 Fr.84 604,8 0,000 0,000 0,000 0,000 0,000 0,000 Fr.85 612,0 0,000 0,000 1,073 0,000 0,000 0,000 Fr.86 619,2 0,065 0,000 0,000 0,000 0,000 0,000 Fr.87 626,4 0,000 0,000 0,000 0,000 0,000 0,000 Fr.88 633,6 0,000 0,000 0,000 0,000 0,000 0,000 Fr.89 640,8 0,000 0,000 0,000 0,000 0,000 0,000 Fr.90 648,0 0,000 0,000 0,000 0,000 0,000 0,000 Fr.91 655,2 0,000 0,000 0,000 0,000 0,000 0,000 Fr.92 662,4 0,011 0,000 0,000 0,000 0,000 0,000 Fr.93 669,6 0,000 0,000 0,000 0,000 0,000 0,000 Fr.94 676,8 0,000 0,000 0,000 0,021 0,000 0,000
