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Primary Clarification of Very High-Density Cell Culture Harvests By Enhanced Cell Settling

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Figure 1: supernatant volume as a function of time for various iEX matrices; the particle size is denoted in parenthensis in µm; 10% v/v iEX matrix was added in each case; total volume in each case was 24 mL.
Figure 3: supernatant volume as a function of time for different initial cell densities; 10% v/v si-pEi (15 µm) was added either to undiluted or diluted cell culture (solid lines); controls without iEX are represented by dashed lines; total volume in each case was 24 mL.
Table 5: summary of impurity removal during depth filtration of reactor B material that was treated by Ecs and reactor c material that was not treated
Figure 6: Hydraulic data for reactor B depth-filtration cell culture after treatment with Ecs; LEFT) millipore train D0Hc (540 cm2) → X0Hc (540 cm2) → supor 0.8/0.2 µm; ROGHT) cuno train 10m02 (25 cm2) → 60Za05a (25 cm2) → supor 0.8/0.2 µm; flux for all depth filters was 50 L/m2/h.

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Primary Clarification of Very High-Density Cell Culture Harvests By Enhanced Cell Settling

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