Universitas Kristen Satya Wacana
Refleksi 10 tahun Magister Biologi
Kamis, 15 Januari 2015
Universitas Kristen Satya Wacana
Venny Santosa
Perwakilan alumni MB
Fakultas Biologi UKSW Class of 1998 (1998-2002)
~2006 worked in consumer goods companies
Double-degree program Magister Biologi UKSW
Class of 2006 (September 2006-Augusts 2007)
Kwansei Gakuin University, Kobe, Japan September 2007-March 2009
On scholarship
= FREE education
September 2007-March 2009
Kwansei Gakuin University, Kobe, Japan April 2010-March 2013
Kwansei Gakuin University, Kobe, Japan April 2009-March 2010
Kwansei Gakuin University, Kobe, Japan April 2013-September 2013
Traveling!!!
Research and
publication
Determination of research topics and goal
Browsing of published data
Research designs; hypothesis
Action!
Idea !
Action!
Progress evaluation and adjustment/alteration
Data analysis and peer review
Publication : paper and seminar
Paper draft writing and revision, grammar check
Application
Overview of Tanaka Lab Laboratory
Science and Technology Fac., Bioscience Dept.
Kwansei Gakuin University
Members: 1 Prof, 1 secretary, 8-15 students (undergraduate-postgraduate), PD
Working hours: 9-17 (official), Mon-Fri (official)
Weekly seminar: progress report and journal club
Seminar with other lab: 1-2x /week
Discussion : 1-2x/week
Task per month (every member):
2x progress report (every 2 weeks), there has to be NEW DATA
1 journal club (3-4 papers)
1 seminar with other lab
Japanese working ethics
Attitude Time to come to work early
Start to work on time Working style hectic
Time to go home late, work long hours Go home early embarrassed
Tanaka Sensei’s working hours:
Mon-Fri : 8.30 – 19.00/19.30 + 23.00 – 2.00
Sat (Sun): 8.30 – 17.00
Death from overwork
Go home early embarrassed
Hardwork appreciated, admired Social pressure high
Ideology live to work' Work on weekend normal
working long hours = sign of diligence and hardworking attitude
idle person = useless and incompetence person
mating
Conjugation
Mei S phase
Meiosis I
Meiosis II
Sporulation
Sample of experimental design (I)
Crossing/mating (day 1) 5 min/plate
Incubation period (1-2 day, check daily)
Pick 25-40 mature sacks/plate (0.5->1 h/plate) Incubation period (overnight)
Separate spores (0.5->1 h/plate)
Mon
Tue/Wed
Wed/Thu
Fri
Sat
Sun
The meiotic cycle of
Schizosaccharomyces pombe
Separate spores (0.5->1 h/plate)
Wed/Thu
3-5 plates
25C 34C 6h
mcb1
mcb1L254P
The DNA damage by heat in temperature-sensitive mutants
Grow cells in 25C (2 strains)
Take YFP photos with fluorescence microscope
Move to 34C
Incubate for 6h Take YFP photos
Sample of experimental design (II)
% cel ls w ith Rad 22-Y FP si gnal
mcb1 mcb1L254P mcb1 mcb1L254P
25C 34C 6h 0 10 20 30 40 50 60
70 1 focus 2/more foci
mcb1L363P mcb1L363P Take YFP photos
•Repeat experiment 3x
•Count cells with 1 focus and 2/more foci
•At least 200 cells/experiment
Academic community
•
Professor and Lab members (junior and senior, PD, secretary)
•
Other professor and their lab members in the same research field
•
Neighboring labs
Social life
•
Seminar
•
Invited presentation
•
Lab trip
•
Research at other lab
•
Research at other lab
