Induce polyploidy of Lilium longiflorum Thunt. Using oryzalin treatmen.

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@

19.

4Ih INTERNATTONAL CONFERENCEON BIOffIENC ES AND BIOTECHNOLOGY "Advancinglife sciencesfor health (oli agingbilqment in _Wrticular)andfodseadty"

COMPARING EXTRACTION METHODS TO ISOLAIE ANDROGRAPHOLIDE FROM

THE BIT"IER HERB @neryraphis

pticulata

Burm.f Ness)

Ni Kadek Warditiani and SrnridjiyoPramono

POSTE R PRESENTATIONS : BIODIVERSITY AND ENVIRONMENT

I.

ALLELESVARIATIONOFCOCONUTACCESSIONS

.

(Cocos nucifera L.,Arecaceae) BASED ON MICROSAIELLITE DNA

Eniek Kriswiyanti, I Gede Rai Maya Temaja, Made Sudana and G.N.

Alit

Susanta Mryra ...-.

GENETIC DIVERSITY OF SOROH CELAGI (PASEK CATUR SANAK CLAN)

BASED ON Y-CHROMOSOMAL MICROSATELITES DNA.

I Ketut Junitha, Made Pharmawati and Wayan Rosiana

TF{E STUDY OF SOCIO-ENGINEERTNG OF SUBAK SYSTEM DEVELOPMENT

WI TH Agro-ecotourism ORIENTED

BIOCATALYTIC DESULFTJRIZATION OF DIBENZOTHIOPHENE

BY Pseudomonas sp. STRAIN KWN5

Ida Bagus Wayan Gunam, I GustiAyu Lani Triani, Nyoman Semadi Antarq Agus Selamet Duniaji, Yohmes Setiyo and DewaAdi Supata

INDUCE POLIPLOIDY OF Liliunlongiflorum THUNB. USING ORYZALIN TREATMENT

Gusti Ayu Mirah Dwidaputri, Eniek Kriswiyanti and Ida Ayu Astarini

COMPOSNNG ACCELERANON OF KITCHEN WASTE USING

MICROBIAL INOCULANT

2.

3.

4.

5.

6.

.7

8.

235

24s

250

254

,t

Ni Made Indri Hapsari Arihntana,Ni Luh Yulianti

...:...

257

HEAVY METAL BIOREMEDIATION

LEAD

(Pb) TO AGRICULTURAL

LAND

ON THE EDGE OF LAKEBUYAN BALI

WITH

PLANT Sanseaieria lorentii

Ni

Luh

Suriani.-...

262

THE IDENTIFICATION

AND

INVESTIGATION OF PARASITIZATION RATE

OF PARASITOIDS OF HAIRY CATERPILLAR LYMANTRIA MARGINATA

WLK.

(LEPIDOPTERA: LYMANTRIIDAE)

IN

BULELENG

BALI

I Wayan Susila

And

I Putu

Sudiarta...-...

...----

'

263

POSTER PRESENTATIONS:

I{EALTH

1.

THE IMPLEMENTATION OF MODULE-JIGSAW COOPERATIVE

LEARNING

STRATEGY

MPROVING

THE PREVENTATIVE BEHAVIOR TOWARDS

DENGUE HEMORRHAGEIC FEVER

Dewa Nyoman Oka

CLASSIC HODGKTN'S TYMPHOMA SIMULATING ANAPLASTIC LARGE

CELL

LYMPHOMA

I Gusti

Ayu

Sri Mahendra Dewi...

EXTENDING TIME OF KOMBUCHA TEA FERMENTATION IMPROVES THE

GROWTH OF

INHIBION

CAPACITY OF Escherichia coli; THE PRODUCER OF

EXTENDED SPECTRTIM BETA LACTAMASES (ESBL) BY

IN

VITRO METHOD

Ni

Putu Aryadnyani, I Dewa Made Sukrama, I Wayan Putu Sutirta Yasa..-..-.--.---.... 277

Bali - Indonesia | 2lo '2Z'd kptember 2012

3.

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&h INTER}LATIONAL CONFERENC E Oi BIOSCIENCES AND BIOTECHNOLOGY '.ldvancing _liJe_{sciencesfor health (anti aging development}_in_particalar)_and

food searity"

CONCLUSIONS

The conclusion that can be drawn from this research were: At 37oC and pH 7 can provide optimal conditions for growth of strain KWN5 with OD values at a wavelength of 660 was I .2 and 200 ppm can degrade DBT in TD was 75.21%.

Pseudomonos sp. Strain KWN5 was great potential in degrading aromatic sulfir compound contained

in petroleum,

it

is necessary to conduct further research especially to choose an effective method in its

application in industry.

REFERENCES

Furuya T, Ishii

I

Ken-ichi Noda, Kino,

K

and Kirimura

K.

2003. Thermophilic biodesulfurization

of

hydrodesulfurized light gas

oils

by Mycobacterium

phlei WU-FI,

FEMS Microbiologt Letters

221:137-142.

Guerinik K2 and Al-Mutawah _{Q. 2003. Isolation}and characterization of oil-desulfurizing bacteria. World

Journal of Microbiologt and Biotechnologt 19,941-945.

Gunam IBW, Yaku

Y

Hirano M, Yamamura K, Tomita F, Sone T and Asano K. 2006. Biodesulfurization

of

alkylated forms of dibenzothiophene and benzothiophene by Sphingomonas subarclicaTTb. Journql

of B i o s c i e nc e and B i o e ngi ne er ing l0 l, 322-327 .

Gunam tBW, Duniaji AS, dan Triani IGAL.2009. Biodesulfurisasi minyak bumi dengan menggunakan

bakteri pendegradasi sulfur dengan teknik sel terimobilisasi. Laporan Penelitian Hibah Bersaing

Tahap II. Universitas Udayana.

Gunam IBW, DuniajiAS, and Triani IGAL, and

SitepuA.20ll.

Biodeslfurization of dibenzothiophene by

growing and immobilized cells of KWN5 strain. 3'd International Conference on "Maintaining World

Prosperity Through Bioscience, Biotechnology and Revegetation", Denpasar, Bali, Indonesia,2l-22

September 2011.

Jasrizal DC. 2009. Deskripsi Dokumen: Biodesulfurisasi Minyak bumi Sebagai Upaya Pengurangan

Pencemaran Lingkungan. Tesis S2. http://www.digilib. ui.ac.i dllopaclthemes/libri2ldetail jsp?id:92

172&lokasi:lokal. Accessed 28 February 2010.

Kirimura K, Furuyal Nishii Y, Ishii Y, Kino K, and Usami S. 2001. Biodesulfurization ofdibenzothiophene and its derivates through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic

bacterium Bacillus subtilis WU-S28. J. Biosci. Bioeng. 9l:262-266.

Laras BK. 2006. Pencemaran Oleh Hujan Asam dalam Konteks Kebijakan Global. http://www.rudyct.

c om/PPS 702 -ipb I 12 1 67 lbambang_kl.pdf. Accessed 3 0 J une 20 1 0.

Schiller JE, and Mathiason

DR.

1997. Separation method

for

coal-derived solids and heavy liquids.

Analyt i c al C hem is try 49, 1225 - 1228.

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@1!Xfi::#tr"",!l:::;:f

x,,x|:.f

::;!ifl

"'#f.!'lil,:'!',?:"1',':3i',ff

:'."',''*-INDucE

poLIpLoIDy

oF

Litiam tongifiarumTHu

N B. u SING

0RYZALIN

TREATMENT

Gusti Avu II{irah Dwidaputrir' Eniek Krl;wiVallir'1nd^ lda

Avu Astarinil

',,"t,s,';;;'H:;i'";;;i;';rt;'!:'i;i;i:[;:ii,!:;*"

udavana universi'cv' 6a"

ABSTRACT

';:;ihltr;{,t:^.}Hl,r#:fi

*t[#:::ffi #"i;i#:'ir:,'nltr{&,;'ffi

ill[ii.I""'*..-il;;.,iiH-;;

create diversitv is via poliploi-dvzauon

and proidy r"r"r or.n o*o,

i^"

i-[

i"rg,ni,rrrft*;;;;ith

orvzalin o'iii"i"

*l

0'0]'v:

R'*Ich

rvas conducted

at Biotechno,ogy

r_uuo,u;';;Ts,;;;;i{;*'""

Tj,lffiI,ih,:}il,i,,.ffi*ial?fffr"fl.,ifi?:i:iffiil:'"'1",il:

University. Treated root ipt

*"tI

fixed with'Farmll''t-,

"*u*,.n* image of the

tttt"*"t"*t'

Resuis show that chromosome type roots were then

,o,r.,

*i,1"o""i"-ot""in 27" ll

lllul'

;;ilil";nric,

whereas oryzalin

0'005%

*#:,'xtffi;"*:ffi*T.?J3iry:x",mrmH;'a"mxJ'iup"'*"0*ongtreaments;ie

stem'YU

and J on control and oryr;,'," o.oor*o*t n.nt, *t'ir-tG-tin',o'oty'

u"u*""t;;il;;;t"'

ttttp"' Chromosomes number on control prants are 24,

ffiffi;;il*,

*iu

ory*'rrid.ffii?"

r..rr,ingin 48

.tili'toti'"'

und treatrnent rvith oryzalin 0'01%

resulted in 32 chromoso;::.

il;il;.",

oo-i,"1.'o;;;;;;;oubled

chromosome in trumpet lilv.

Keywords: chromosome' mutation' trumpet lily

INTRODUCTION

Lilium(Liliumlongiflorum*To,familyLiliaceaepopularlyknownasEasterLilyorwhite

trumpet

lily.

Trumpet

lilyls

a peren,"l.r^1*

wttt'io'

3

- 4

yearproduction time' Trumpet lily

commonly

found in mountainJous areas but can utro

tir"

in

muddy areas (Marlina' 2009)'

Lilium

is a high value cut

flower;;;;

the

world'

pemand for international market increase

around26ohperyear]lti'tt'"fifthmostl'p"n**'flowerintf"N"tt'"tlandwithprodcutionmorethan

300 milrion

,r"nl

p". year (Van Tuyl dan

r#;il;;,

rqgo-1,

ttt"

usA,

1l _'5million lilium bulb was

produced

*o

r"uJJ'rJ;

r;;;rd,h";;;. ;

r'ndon"riu,

riri'*

p'"a"ction is relativelv row

so that

domestic market

iJ.ti,-r"ry

on

importediiii"*.

r-"*r

lily

value ai artund Rp 7.500 per srem' Imported

lily

vatue u,

uroui

*r.liioop:i:*T

(w;;,

200e)'

Low

o';;;;'

oriitv

in lndonesia mainlv due

i:1":-,"fii1*fu'll'ff;111il::#:ffiiil#:L',ll::*j:l::f::ff"T;i'lv

rnducemu'1a'ii'n

is become

u,

irnpoiu*1ool

to obtaln t igt, uutu.

superior curtivars

uni

_"unbe done using chemical

and

physical

,uru*"rl'o

i^tini.

u

"r,"*'i?'j,ffi;il;"i

"Urt.t"lt'a'ce

poliploidv'

oryzalin stronglv

bind tubulin

i,

pt*t

ro ro.rn u

"o*pt"*Lr-u,iJ-5o-""

,,'tt;;;lex

do not able to polimerize into

microtubulu,

,o

i

inhibit ce,l division

on'-'i'

ha' b""n

u'"

to obtain new genotype with variation

on

flower una t"ur

rir"

and colour

oroortulii.

ioool

van Tuyl '",

"i

-

onr,>dicribe

that tetraploid lilium

;ffi g'":*1;x:#:;[T"'j!Tji,E]l'iJ:'"ffiffi

o:'"T",::\:,:u'*tonginorum

Thunb. after induction using oryzalin o.ooiy"

and 0.01%.

t"rl* r"r*

benefit is to provide

more variety

in Lilium'

MATERIAI,S AND

METHOD

B i otec hno lo gv L aboratory'

D : p1n*

t

::

:'

i:t]::::T"'

:,*];"ili

t#$

,,*",I::X:',:fr

,ffi:,l,#:":"l.1"):I=T::i:*111;;'*l,l*;;;;X;Jfl:?*ffi

Each bulb

i

^T:1:i::rx::i:'#i:;:i:"#'#TT"::r:i',r;1.",-;;'-Xl,:;I,lll';"ii

*:!,-i",";v;;#T:Jili:!,!:"1fi#:#,?"i{ti}::x;;iH;i;TH

was planted

* p!vb'qr;:T::ff

}Tffi;ffi},[i;l

1*

"l

r,oi,.'

Bulb were trren moved to water

in Oryzalin solution wt

(5)

E

ly

6e

Bn 5AS

bt

iled

due

lfr -tifLst {fio.\--l L co\-FEREicE on

amrlt\cEs

iliD BIoTECH\:OLrcl

Al

--lha

tfr

ws lr

tcatth

bri

aging

&vlwnr

tu _wtiL-ubl@d

Id

secttiry "

W

srt.;:on lor

l-t

hours to remove residu

\,rash

method rvas med to examined chromosome of Lilium longiflorum after treatrnent. Root

rps aisre cL[

=

1

mm at 7 am to obtained best stage of the chromosome. Treated root tips were fixed n:rh Farner soluriorr. followed by hydrolisis with 2N HCI prior to root squash. Squashed roots were then

mrm6d

rli*

Aceto-orceinT/o for 30 minutes, flame over Bunsen btnner to obtain contrasting image

of

&c circrnoscrne (Ahmad a at-,1983). Chromosome counting and karyotyping was done on l0 cells with rmefiry&ffie a:rd pro-metaplroe stage using Olympus Light Microscope. Photograph of the chromosome

nES:rk€n using Digital Camera Kodak M863.

RESULTS

AND

DISCUSSION

Results show that Oryzalin treatments gave variation in term of size, type, form and number

of

ctrnxnosome. Control plants has chromosome size ranging 32,5 pm- 73,8

pm.

Treatment with Oryzalin

[r-O05o.,'i produced chromosome size26,3 pm - 80 pm, while Oryzalin0.0loA produced chromosome size

i6i

pm - 68,8 pm.

Control plants have the same type

of

chromosome with plant treated with Oryzalin 0.017o, i.e.

rnetacentric and submetacentric, whereas Oryzalin 0.005% treatment shows metacentric, submetacentric arrJ subtelocentric

type.

Chromosome shape varied among treatmen8; i.e. stem,

Y

U

and J on control

ayrd Ory'zalin 0.005% treatment, while Oryzalin 0.01% treafinent resulting in stem shape. Morphometric rariation on chromosome occurred due

to

Oryzalin induced mutation, causing chromosome structure aberration. Type ofaberration that can occur includes deletion, duplication, inversion and translocation

r Broun and Caligari 2008).

Chromosomes number on control plants are 24, whereas treatment with Oryzalin 0.0050/, resulting in

{8

chromosome, and treatrnent with Oryzalin 0.01% resulted in 32 chromosomes (Figure 1). This was

in accordance with researchresults by Takamura (2002) which revealed the most effective ploidy induction on in vitro Lilium using Oryzalin was at 0.005%. However, optimum concentration to produce bulblet and highest ploidy level was at 0.003%. Higher concentration inhibits bulblet formation on L. longiflorum

erplants.

Llc

Figure 1. Lilium Chromosome. a. Control, b. Oryzalin 0.005%, c. Oryzalin 0.010

lion

I and mgl-v

r into on on

$tum

iflorum

nariety

rd

Plant

$_May

tach bulb

m

soaked

d to rvater

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4th INTERNATIONAL CONFERENCE ON BIOSCIENCES AND BIOTECHNOLOGY

..Advancing _life,ri"nr"ryo,

fra*t,nii";siii

ar"ropmenr in paictrrar) andfod securitv"

CONCLUSION

In

conclusion, Oryzalin 0.005% effective to doubled chromosome

in L. longifurum, from 24 on

untreated plant to +s on

i."ut

d plants' There are 3

types

of

chromosome found'

i'e'

metacentric and

submetacentric (on contror plants and oryzalin 0.01yo,0.005% treatment),

and subtelocentric type only on

Oryzalin 0-005% treatment'

REFERENCES

Ahmad QN, Britten EJ and Byth DE. 1gg3. A Quantitavive Method

of Karyotypic Analysis Applied to

the Soybean, Glycine max. Departm"ni

i

Genetic and Plant Breeding'

Bangladesh Agriculture

Department, Mymensingh, Bangladesh'uJ

,"p*ent

Agriculture', University of Queensland' St

ur"*JrT;:3}ii;I?Srllitffili"

rntroducrion to prant Breeding. Brackweu

publishing

Ltd'

oxrord'

UK.

Marlina N. 2009. Teknik perbanyakan

Lili

Dengan Kultur

Jaringan. Buletin Teknik Pertanian

vol'

14 No'

**.",1;?l?rl;l

f'lffiraishi

M.

2000. rnduced polvploidv in Grapes vra

in

virro chromosome

Doubling. J.

"f

;;;;

aociety of Horticulture Science 69(5): 543-55 I .

Takamura T, Lim KB and van Tuyl JM. 2002. Effect of aNew compound

on the Mitotic Polyploidization

of

Lilium longiflorumand oriental Hyu'ia

titl"s'

Proc'

xx

EUCARPIA Symp'

onNew

ornamentals

,r",

r.1iffi:,ffT::Htl['*';T;'i1

.,

*

2.rheuse

ororyzarin as an Arternative ror corchicin e in in

vjrroChromosomeDoublingofLiliumandNerine.ActaHort'FlowerBulbs.325:626-627.

Van Tuyl JM and

v*

n"ir,"iin

H'ctut. lgg6. Lily Breeding Research

in the Netherlands. proc. Intl. Sym'

on Lilium' Acta Horticulturae 414: 35-36'

wuryan. 2009. analisi, rinurriut Usahatani

Lili

Lokal dan

Lili

Impor'

http://wuryan'wordpress'

com /200g/0 t/zslanalisis-finansial-usahatani-lili-lokal-dan-lili-impor/