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Nur Hi dayat i
Committee Chair Committee :
Dwi Fajar Saputra, M.M (UPNV Jakarta- Indonesia) Scientific Committee :
Andri Pramesyanti, Ph.D (UPNV Jakarta – Indonesia) Ifdil, Ph.D (Universitas Negeri Padang - Indonesia)
Dr. Aditya H.P.K Putra (Universitas Muslim Indonesia - Indonesia)
Dr. Prantasi Harmi Tjahjanti, M.T (Universitas Muhammadiyah Sidoarjo - Indonesia)
Fredy Kurniawan, ST, MT, M.Eng., Ph.D (Universitas Narotama - Indonesia) Dr. rer. nat. Bintoro Anang Subagyo (Institut Teknologi Sepuluh Nopember - Indonesia)
Ahmad Fudholi, Ph.D (Universiti Kebangsan Malaysia - Malaysia)
Organizing Committee :
Mochammad Tanzil Multazam, M.Kn (Universitas Muhammadiyah Sidoarjo - Indonesia)
Andista Candra Yusro, M.Pd (Universitas PGRI Madiun - Indonesia) Busro, M.Ag (UIN Sunan Gunung Djati Bandung - Indonesia)
Misbah, S.Pd., M.Pd (Universitas Lambung Mangkurat - Indonesia) Robbi Rahim, M.Kom (Universiti Malaysia Perlis - Malaysia)
Utama Alan Deta, M.Pd., M.Si (Universitas Negeri Surabaya - Indonesia)
Amipriyadi Gerha Permana, S.IP (Universitas Pancasila - Indonesia)
Welcoming Note
We are delighted to introduce the 2nd Annual Conference on Health and Food Science Technology with theme “Covid-19, Food Security and Panic Buying”. The technical program has brought researchers and practitioners around the world to a good forum for discussing, leveraging and developing all scientific and technological aspects that are relevant to technology, food and biology. Moreover, it is with a great pleasure to have the keynote and invited speakers of 2nd ACHOST, Dr. Wahyu Caesarendra and Andri Pramasyanti, Ph.D who will share their knowledge and best innovative research findings.
This conference is held by Kresna Acitya Media Nusantara in collaboration with Relawan Jurnal Indonesia. Kresna Acitya Media Nusantara is a company that dedicated to maximize impact of scientific publication, and Relawan Jurnal Indonesia, is a non- profit organization in field of scientific publication. The conference will be held virtually using Zoom webinar on November 25, 2020. This conference was successfully acquire 150 participants and 71 presenters. Thus, all selected papers will be submitted for publication to our publishing partner IOP. We hope that the future ACHOST will be as successful and stimulating, as indicated with the contributions presented in this volume.
Yogyakarta, 23 November 2020 Conference Chair
Dwi Fajar Saputra
Rundown
Time Event PIC
08.00 – 08.30 AM Waiting Room Zoom PIC Event
08.30 – 09.00 AM Preparation PIC Event
09.00 – 09.15 AM Opening Remarks Director Kresna Nusantara 09.15 – 09.30 AM Information About
Publication Publication Chair 09.30 – 10.30 AM Technology Role Playing
Within COVID-19 Dr. Wahyu Caesarendra (Universiti Brunei Darussalam) 10.30 – 11.30 AM Antimicrobial and antivirus
potential from natural product
Andri Pramesyanti, Ph.D (Universitas Pembangunan Nasional Veteran Jakarta)
11.30 – 12.00 AM Discussion Moderator
12.00 – 12.30 PM Closing Moderator
Table of Contents
Article ID Title of
Abstracts
AR-0003 The Effect Of Indonesian Honey Tetragonula Sp. And Indonesian Royal Jelly Apis Mellifera (Ceiba Pentandra) To Human Preputium Cell Proliferation In Serum-free DMEM
AR-0005 The Relationship Between The Use Of Rational Antibiotics With Body Temperature In Patient With Pneumonia
AR-0006 The Role Of Diffusion Of Innovation In Agricultural Business To Compete In Economic Asean Community
AR-0008 Material and Service Life of The Septic Tank Have an Influence On The Biological Pollution Of Groundwater (Case Study Kelurahan Pademangan Barat, North Jakarta)
AR-0009 Green Open Space Implementation On The Underground Building AR-0015 Social Consideration For Blue Carbon Management
AR-0017 The Use Of Bioremediation Technology On Oil-Contaminated Soil AR-0018 The Strategy Of Conservation Development For Cacatua Sulphurea In
Bontomarannu Education Park, Gowa Regency Animal Park.
AR-0019 Somatic Embryogenesis Of Todolo Toraja Coffee Leaf Cells (Coffea Arabica Var. Typica) With The Addition Of 2,4-Dichlorophenoxyacetic Acid (2,4-D) And 6- Furfurylaminopurine (Kinetin) In Vitro.
AR-0021 Isolation, Characterization, Molecular Identification Of Probiotic Bacteria From Meconium
AR-0023 Analysis Of Changes In Health Of Coastal Mangrove Forest On The East Coast Of Lampung
AR-0027 COVID-19 Human Movements Monitoring System
AR-0032 Proteolytic and Clot Lysis Activity Screening of Crude Proteases Extracted from Tissues and Bacterial Isolates of Holothuria Scabra AR-0033 Actualization Of Local Community Participation In Critical Land
Management In Gorontalo
AR-0034 Meistera chinensis fruit properties: Chemical compound, antioxidant, antimicrobial, and antifungal activity
AR-0037 Natural Antibacterial And Its Use
AR-0038 Collaborative Empowering Model of Dry Lands Farmer (Case Study West Halmahera District, North Maluku Province)
AR-0040 The Effect Of Temparature Change On Ant Diversity In Haruku Island AR-0042 Acanthocephaliasis And Sparganosis Occurrence In An Asian Vine
Snake (Ahaetulla Prasina): A Perspective Of Neglected Zoonotic Disease
AR-0043 Specific Immunomodulatory Effect Of Water Extract Of Stachytarpheta Jamaicensis Herbs
AR-0044 Antihypertensive Effects Of Purslane (Portulaca Oleracea) Extract In Animal Model Of
AR-0046 Application Of Supply Chain Requirements For Smallholders: Impact On Sustainable Palm Oil Management Policies In Indonesia
AR-0047 Papalele's Household Economic Dynamics In Increasing Income AR-0048 Analysis Of Chemical Quality Of Dried Sendfish Sea Cucumber
(Holothuria Scabra) Using Different Enzyment Methods
AR-0049 Epidemiological Characteristics Of The First Cluster Of Covid-19 In Magetan District – East Java, 2020
AR-0053 The Effect Of Microwave Aid Extraction Temperature Of Kedawung Leaves (Parkia Biglobosa) On Antioxidant Activity And Flavonoid Levels
AR-0054 Variation Of Nutrition Intake For Pregnant Women In Tembuku Region
AR-0055 Literatur Review: Potential Pharmacological Activity Of Luffa Acutangula L. Roxb
AR-0057 Effect Of Ammonium Chloride And Disodium Hydrogen Phosphate With Molasses As A Substrate For Saccharomyces Cerevisiae In The Production Of Β-Glucan.
AR-0060 Twenty Weeks Of Centella Asiatica Improved Cognitive Function Of Women Elderly With Dementia
AR-0064 Bontomarannu Education Park Reviewed From Conservation, Social, And Environmental Aspects
Proteolytic and Clot Lysis Activity Screening of Crude Proteases Extracted from Tissues and Bacterial Isolates of Holothuria Scabra
(AC-0449 Nur Hidayati / Magister Study Program of Medical Laboratory Science, Universitas Muhammadiyah Semarang, Indonesia), (AC-0450 Hayatun Fuad / Magister Study Program of Medical Laboratory Science, Universitas Muhammadiyah Semarang, Indonesia), (AC-0451 Hendra Munandar / Balai Bio Industri Laut - LIPI
(Lembaga Ilmu Penelitian Indonesia) Lombok, Nusa Tenggara Barat, Indo), (AC-0452 Dewi Seswita Zilda / Research and Development Center for Marine and Fishery Product Processing and Biotechnology, Ministr), (AC-0453 Nurrahman Nurrahman / Food Technology Study Program, Faculty of Nursing and Health Sciences,
Universitas Muhammadiyah Sema), (AC-0454 Oedjijono Oedjijono / Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia), (AC-0455 Amin Samiasih / Magister Study Program of Nursing Science, Universitas Muhammadiyah Semarang, Indonesia), (AC-0456 Stalis Norma Ethica / Magister Study Program of Medical Laboratory Science, Universitas Muhammadiyah Semarang, Indonesia), (AC-0533 Miswan Fattah /
Departemen of Molecular Biology Prodia Widyahusada Laboratory Jakarta Indonesia),
Correspondent author : (RJI-1-0046 Stalis Norma Ethica) Universitas Muhammadiyah Semarang Abstract
Cardiovascular diseases (CVDs) are still the leading mortality causes in the last decades. The ailments are multifactorial characterized by excessive clot (thrombus) formation in the blood vessels. Thrombus could be degraded through thrombolysis mechanism by plasmin activated by various fibrinolysis agents including, urokinase, nattokinase, or streptokinase. However, the use of these agents is restricted by relatively high cost, short half-life, allergic reaction, and bleeding effects. The search for more economical and safer thrombolytic (clot lysis) agents are essential to address the underlying problem in CVD therapy. Among Holothurians, H. scabra has been known to have the highest protein content making it ideal substrate for protease enzymes including fibrinolytic types with clot lysis properties. However, isolation of a proteases with antithrombotic activities either from tissue or from bacteria of H. scabra has not been reported. This study aimed to screen proteolytic and clot lysis activities of crude protease extracts from tissue and bacteria isolated from fermented intestine of H. scabra. Crude protease of tissue of H. scabra was extracted by cold centrifugation, which activity was measured using UV-spectrophotometer. Crude protease of proteolytic bacteria selected by clear zones on skim milk agar (SMA) medium was isolated from nutrient broth (NB). Extracts showing proteolytic activity were subjected to gravimetry-based clot lysis test. As results, crude proteases isolated directly from the H. scabra’s tissue showed low proteolytic activities, thus were no proceed to clot lysis activity test. Crude protease extracted bacteria could show both proteolytic and clot lysis activities. In conclusion, based on this screening study, intestine of H. scabra is a rich source of proteolytic bacteria. Some of them could produce crude enzymes showing competitive thrombolysis activities with Nattokinase. Thus, they appeared to have more potentials to be developed as thrombolysis agent than those directly extracted from the organism’s tissue.
Keyword : Holothuria scabra, cardiovascular disease, anti-thrombotic agent, fibrinolytic protease Subject : food and disease