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Lampiran 1. Pembentukan warna pada uji kadar nitrat (A), nitrit (B), dan amonium (C).
A
B
Lampiran 2 Kurva standar konsentrasi amonium (A) dan kurva standar konsentrasi nitrit (B)
Kurva Standar Amonium
y = 11.201x + 0.0514 R2 = 0.9963 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 Konsentrasi amonium (mM) A bs or bans i ( 640 nm)
Kurva Standar Nitrit
y = 114.81x + 0.224 R2 = 0.9087 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 0.000 0.002 0.004 0.006 0.008 0.010 0.012 0.014 0.016 Konsentrasi nitrit (mM) A bs or ba ns i A B
Lampiran 4 Kurva standar pertumbuhan isolat HF7 dan LF6 pada kondisi nitrat medium 49,41 mM
Kurva Standar Pertumbuhan Isolat HF7
y = 0.0073x + 0.0208 R2 = 0.9623 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0 10 20 30 40 50
Jumlah Organisma ( 109CFU/ml)
O
D
(620
nm)
k urva s tandar pertum buhan Isolat LF6
y = 0.091x + 0.0239 R2 = 0.9427 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0 5 10 15 20 25 30
Lampiran 5 Presentase nitrat tereduksi dan senyawa yang dihasilkan isolat LF6,HF7 dan FR2 pada kadar udara saturasi berbeda dengan inkubasi selama 4 hari suhu ruang (29-31)oC
Isolat LF6 Nitrat Awal nitrat tereduksi Nitrit terbentuk Amonium terbentuk Estimasi gas terbentuk Perlakuan udara saturasi mM % mM % mM % mM % 0% 49,41 48,52 98,19 0,05 0,00 15,02 30,95 33,45 68,95 1% 49,41 47,78 96,69 0,00 0,00 6,17 33,85 31,61 66,15 10% 49,41 47,50 96,12 0,02 0,00 8,94 18,83 38,54 81,14 30% 49,41 47,17 95,46 0,01 0,00 9,26 19,63 37,90 80,35 50% 49,41 46,04 93,17 0,04 0,00 12,00 26,06 34,01 73,86 80% 49,41 45,34 91,75 0,03 0,00 10,02 22,09 35,29 77,84 100% 49,41 45,40 91,88 0,03 0,00 9,18 20,23 36,19 79,71 Isolat HF7 Nitrat Awal nitrat tereduksi Nitrit terbentuk Amonium terbentuk Estimasi gas terbentuk Perlakuan udara saturasi mM % mM % mM % mM % 0% 49,41 48,78 98,72 0,00 0,00 14,77 30,29 34,01 69,72 1% 49,41 47,07 95,26 0,00 0,00 14,19 30,14 32,89 69,86 10% 49,41 46,13 93,35 0,00 0,00 13,15 28,50 32,99 71,51 30% 49,41 47,17 95,46 0,01 0,03 14,18 30,06 32,98 69,91 50% 49,41 45,83 92,75 0,02 0,04 16,19 35,33 29,62 64,63 80% 49,41 45,84 92,77 0,00 0,00 14,69 32,05 31,15 67,95 100% 49,41 45,67 92,42 0,03 0,07 7,29 15,96 38,35 83,96 Isolat FR2 Nitrat Awal nitrat tereduksi Nitrit terbentuk Amonium terbentuk Estimasi gas terbentuk Perlakuan udara saturasi mM % mM % mM % mM % 0% 49,41 48,37 97,88 0,00 0,00 6,73 13,91 41,64 86,09 1% 49,41 46,63 94,36 0,00 0,00 9,02 19,35 36,91 79,16 10% 49,41 46,84 94,78 0,02 0,04 9,20 19,65 37,61 80,31 30% 49,41 48,31 97,76 0,00 0,00 8,35 17,28 39,96 82,72 50% 49,41 47,89 96,91 0,00 0,00 8,90 18,58 39,77 83,05 80% 49,41 47,47 96,06 0,00 0,00 10,19 21,47 38,87 81,88 100% 49,41 44,76 90,58 0,03 0,06 7,72 17,25 41,38 92,44
Lampiran 6 Presentase nitrat tereduksi dan senyawa yang dihasilkan isolat FR1 pada kadar udara saturasi berbeda dengan inkubasi selama 4 hari suhu ruang (29-31)oC Isolat FR1 Nitrat Awal nitrat tereduksi Nitrit terbentuk Amonium terbentuk Estimasi gas terbentuk Perlakuan udara saturasi mM % mM % mM % mM % 0% 49,41 48,20 97,54 1,74 3,61 7,09 14,71 39,37 81,68 1% 49,41 47,73 96,58 1,88 3,94 9,05 18,97 36,79 89,79 10% 49,41 48,01 97,15 2,86 5,96 6,87 14,30 38,28 85,87 30% 49,41 47,28 95,68 2,55 5,39 6,08 12,86 38,65 87,14 50% 49,41 47,08 95,28 2,54 5,39 6,55 13,92 37,99 86,08 80% 49,41 46,35 93,79 3,17 6,84 6,75 14,56 36,43 85,44 100% 49,41 46,26 93,62 4,95 10,70 6,50 14,04 34,82 85,96
Lampiran 7 Perbandingan konsentrasi nitrat tereduksi, nitrit, nitrat, amonium dan estimasi gas yang dihasilkan isolat LF6 dan HF7
Keterangan Nitrat tereduksi Nitrit terakumulasi
Amonium yang dihasilkan Estimasi gas yang dihasilkan
\ Isolat LF6 0 10 20 30 40 50 0% 1% 10% 30% 50% 80% 100%
Konsentrasi udara saturasi
N it ra t, n it ri t, amo n iu m, g as (mM ) Isolat HF7 0 10 20 30 40 50 0% 1% 10% 30% 50% 80% 100%
Konsentrasi udara saturasi
Ni tr at , Ni tr it , Am on iu m , ga s (m M )
Lampiran 8 Perbandingan konsentrasi nitrat tereduksi, nitrit, nitrat, amonium dan estimasi gas yang dihasilkan isolat FR1 dan FR2
Keterangan Nitrat tereduksi Nitrit terakumulasi
Amonium yang dihasilkan Estimasi gas yang dihasikan
Isolat FR2 0 10 20 30 40 50 0% 1% 10% 30% 50% 80% 100%
Konsentrasi udara saturasi
Ni tr at ,n it ri t, am o n iu m ,g as (m M ) Isolat FR1 0 10 20 30 40 50 0% 1% 10% 30% 50% 80% 100%
Konsentrasi udara saturasi
Ni tr at ,n it ri t, amon iu m , g as (m M )
