DETECTION OF HPV 16 AND 18 FROM ANAL SCRAPING OF MALE SEX MALE BY PCR METHOD IN BALI
IBN. Putra Dwija1). I Ketut Agus Somia2). Made Yogi Oktavian Prasetia3, Ketut Tuti Parwati Merati2)
1). Department of Clinical Microbiology, Faculty of Medicine Udayana Unyversity.,2)Division of Tropical and Infectious Diseases, Department of Internal Medicine Faculty of Medicine Udayana
University.,3)Bali Medika Clinic Kuta Corresponding author:[email protected] Background
Human Papilloma Virus (HPV) is the common agent of wart at different part of body including hand, neck, cervix and the area between anus and genital. More than 100 type of HPV has been isolated and some of them are grouped into High Risk HPV (HPV-HR) which is potentially to develop cancer. HPV HR especially HPV 16 and 18, is the commonest type cause cervical and anal cancer. Male sex male (MSM) is the group at a increasing rate to develop anal cancer that caused by HPV HR.
Material and Methods.
Anal scrapings were collected from the patient who comes to clinic to do routine screening. Anal scraping was collected by sterile Dacron swab by the physician, and the specimen than placed to the micro tubes which is added with 1 ml of phosphate buffer saline (PBS) and send to the Biomolecular Udayana University laboratory for further analysis. DNA extracted by Promega® Extraction Kit and PCR using MY 09/11 primers pair and HPV 16 & 18 specific primers pair. Result
During 3 months of specimens collection, 34 samples was collected. Among them 26 (76,5%) were positive infected by HPV and 8 (24,5%) were negative. From the total positive samples, HPV 16 was detected in 11 (42,3%), HPV 18 was detected in 10 (38,5 %), dual infection was detected in 5 samples (19,2%).
Conclusion
Increasing detection of HPV among MSM patient needed more attention for routine anal pap screening and treatment should be integrated intoanal cancer prevention andcare program for
MSM.
