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Short communication

Population patterns of

Aspergillus ¯avus

group and

A. niger

group in ®eld

soils

G.J. Grif®n

a,

*, E.P. Smith

b

, T.J. Robinson

b

aDepartment of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA bDepartment of Statistics, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA

Received 9 June 1999; received in revised form 25 January 2000; accepted 20 June 2000

Abstract

The horizontal distribution of soil-borne fungi in ®eld soils is an important component of fungal ecology.Aspergillus ¯avusgroup and its antagonist, theA. nigergroup, had moderate and moderate to high degrees of aggregation (patchiness), respectively, in two peanut ®elds with a history of a¯atoxin contamination: Lloyd's index of patchiness values ranged from 2.32 to 6.55, where 1.0 is a random pattern. For theA. nigergroup, there was evidence that quadrats in close proximity had similar population densities. Clump size, rarely reported for soil-borne fungi, was found to be 13±52 m2forA. nigergroup in one ®eld. Mean population densities ofA. ¯avusgroup in both ®elds were higher than found previously in most Virginia peanut ®elds and, along with moderate aggregation, may be related, in part, to the occurrence of a¯atoxin in these ®elds.q2001 Elsevier Science Ltd. All rights reserved.

Keywords: Population patterns;Aspergillus ¯avus;A. niger

Information on population densities and horizontal patterns of soil-borne fungi is important to management of pathogens. While information on the degree of aggregation of soil-borne fungi has been presented in recent years (Campbell and Bensen, 1994; Grif®n and Baker, 1991), there is little or no information of the size and location of patches of aggregated (clumped) or high-density inoculum in ®eld soils (Gilligan, 1995; Mihail and Alcorn, 1987).

Aspergillus ¯avusgroup fungi (A. ¯avusLink andA. para-siticus Speare) have been responsible for production of

carcinogenic a¯atoxins in peanuts (Arachis hypogeae L.)

in soils worldwide. High levels ofA. ¯avusgroup

coloniza-tion of peanut fruits in soil and a¯atoxin produccoloniza-tion are favored by hot, dry conditions (Diener et al., 1987; Cotty

et al., 1994). BothA. ¯avusgroup fungi (primarilyA. ¯avus)

and A. niger group fungi may be isolated from peanut kernels, and both may colonize peanut fruits in ®eld soils. These fungi have been considered antagonists of each other by some workers (Ashworth et al., 1965; Joffe, 1969; Diener et al., 1987), and little or no information exists on the rela-tive spatial patterns of pathogens and their antagonists in ®eld soils (Gilligan, 1995). The present investigation was

undertaken to determine the horizontal pattern ofA. ¯avus

andA. nigergroups in two neighboring peanut ®elds with a

recent history ofA. ¯avusgroup colonization and a¯atoxin

contamination of peanuts in soil.

Field plots were established in two commercial ®elds, about 4 ha in size, in Virginia, USA, which contained peanuts that tested positive for a¯atoxin contamination in the previous crop. Peanuts and corn have been grown in these ®elds for several decades. The ®elds were on the same farm and were located about 300 m apart. The ®elds were sampled in June after peanuts had been planted in the ®rst week of May. Weed and disease control were good. A 25:4£25:4 m

2

lattice plot was established in each ®eld and

was divided into 49 blocks, each 3:6£3:6 m

2

:A soil-core

sample (2.5 cm diameter by 7.6 cm deep) was collected at the center of each block. These centric-systematic samples (Milne, 1959), or quadrat samples, were placed in plastic bags with pinholes for gas exchange and transported to the

laboratory. Soils were placed at 4±58C and assayed. Soil

samples were mixed by hand for 5 min previous to dilu-tion-plate assay on M3S2B medium, selective for low

popu-lation densities of A. ¯avus group and A. niger group, as

used previously (Grif®n et al., 1975, 1981). Undesired bacteria and fungi are completely or severely inhibited in

this isolation procedure, which uses Botran (2mg of

2,6-dichloro-4-nitroanaline per ml), NaCl (3%), high

tempera-ture (358C), and antibacterial antibiotics to achieve selective

inhibition. This medium was able to recover at 1021 soil

dilution very low population densities from soil [1±10

Soil Biology & Biochemistry 33 (2001) 253±257

0038-0717/01/$ - see front matterq2001 Elsevier Science Ltd. All rights reserved.

PII: S 0 0 3 8 - 0 7 1 7 ( 0 0 ) 0 0 1 3 3 - 4

www.elsevier.com/locate/soilbio

* Corresponding author. Tel:11-540-231-5049; fax:11-540-231-3221.

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G.J.

Grif

®n

et

al.

/

Soil

Biology

&

Biochemi

stry

33

(2001)

253

±

257

Table 1

Advantages, disadvantages, and results obtained for ®ve statistical estimates, tests, or plots onAspergillus ¯avusgroup andA. nigergroup population densities in two peanut ®elds with a history of a¯atoxin contamination

Statistical estimate, test, or plot

Advantages Disadvantages Results

A. ¯avusgroup A. nigergroup

Field A Field B Field A Field B

Mean population density Provides information on whether high or low overall population densities are present in a ®eld soil

Does not provide information on aggregation, randomness, or location of fungal population densities in a ®eld soil

33.9 cfuaper g

soil

29.2 cfuaper g

soil

318.8 cfuaper g

soil

301.6 cfuaper g

soil

Lloyd's index of patchiness (or similar Morrisita's index of dispersion) (Pielou, 1977; Morrisita, 1959)

Indicates if propagules exist as aggregates or have a random pattern; 1.0ˆrandom pattern and.1.0 indicates increased aggregation

Does not indicate the location or size (area) of aggregates (ˆclumps, patches), if present in a ®eld soil

2.96 2.32 6.55 3.19

Moran'sIstatistic of autocorrelation (Upton and Fingleton, 1985)

Indicates if similar population densities are in close proximity in a plot of mapped data

Choice of scale used in sampling may markedly affect results

0.173 (Pˆ0.0336)b*

zˆ1.823

0.117 (Pˆ0.0986)b*

zˆ1.295

0.517

(Pˆ,0.0001)b*

zˆ5.046

0.373

(Pˆ,0.0011)b*

zˆ3.70 Plot of Morrisita's index of

dispersion versus number of samples in a cluster (Vandermeer, 1981)

Provides information on clump size (area) in a plot of mapped population density data

Does not provide information for speci®c population densities

13±26 M2 13±26 M2 13±26 M2 13±52 M2

Krishner Iyer test (Pielou, 1977)

Indicates if high population densities are randomly distributed in a plot of mapped data

Does not provide information for speci®c population densities

(NRJ)c (P.0.05)*

(NRJ)c (P.0.05)*

(RJ)c(P,0.05)* (RJ)c(P,0.05)*

a cfuˆcolony-forming units. b Values for ranked data.P

,0:0001 andP,0:0011 indicate strong evidence,Pˆ0:0336 indicates marginal evidence andPˆ0:0986 indicates very marginal evidence for samples in close proximity

having similar values.

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colony-forming units (cfu) per g soil] ofA. ¯avusgroup in extensive evaluation tests (Grif®n and Garren, 1974; Grif®n

et al., 1975). Eight dilution plates at 1021or 1022dilution

were incubated at 358C for 3 days after which colony counts

ofA. ¯avusgroup andA. nigergroup (primarilyA. nigervan Tieghem) were made.

Several approaches have been used to study the pattern of soil-borne fungi in ®eld soils (Campbell and Bensen, 1994). Following soil assays, Lloyd's index of patchiness (Pielou, 1977) was calculated in the present study to give an estimate of the degree of aggregation of propagules in soil (Table 1).

Moran'sIstatistic (Cliff and Ord, 1973; Upton and

Fingle-ton, 1985) was used to evaluate whether similar population densities occurred in quadrats (samples) located in close

proximity in a lattice plot. Moran's I for ranked data,

found useful here, is given by:

Iˆ 12

where Xi is the rank of population density quadrat i, n

denotes the number of locations;

S0ˆ4c…c21†;

where c is the number of columns, and Wij the weight

assigned to the join between quadratsiandjusing the rook's

de®nition (quadrats which have touching sides) of contigu-ity (Upton and Fingleton, 1985). The analyses for ranked

and unranked (ˆpopulation density) data are similar; as

indicated by Upton and Fingleton (1985), the equation for

Moran'sIusing ranked data is a simpli®cation of the

equa-tion for Moran'sI using unranked data. In this study, the

conversion of the population density data to ranked data was straightforward (the lowest population density has the lowest rank): thus, the quadrat with the lowest population

density value in the lattice plot for A. ¯avus group or A.

nigergroup was given the rank of 1; the quadrat with the second lowest population density value was given the rank of 2, and the quadrat with the third lowest population density value was given the rank of 3, etc. To estimate aggregate size, Morisita's index of dispersion or patchiness index (Table 1) was calculated (Morrisita, 1959; Vanderm-eer, 1981) for clusters of one to ®ve quadrats, taken down crop rows or across crop rows. Quadrat clusters of increas-ing size were thus obtained. For each data set, Morisita's index of dispersion was plotted against the number of quad-rats in a cluster. A rapid drop in Morisita's index was taken to indicate a fungal clump size that corresponds approxi-mately to the quadrat cluster size at which the rapid drop occurred (Vandermeer, 1981). The relative advantages and disadvantages of these tests for determining population pattern are indicated in Table 1. Other workers have also used Lloyd's index of patchiness (Hampson and Coombes, 1996; Grif®n and Baker, 1991), Morrisita's index of disper-sion (Mihail and Alcorn, 1987; Campbell and Bensen, 1994)

and Moran's I (Mihail and Alcorn, 1987; Campbell and

Bensen, 1994) to study the spatial patterns of soil-borne fungi.

Box plots of the population densities of A. ¯avusgroup

andA. nigergroup in the 49 quadrats of each ®eld were done in order to visualize the pattern and association of quadrat data. Using an approach suggested by Pielou (1977), that incorporated the Krishna Iyer (1949) test, population density data were classi®ed as high-valued and low-valued for each fungal group based on the median population density in each ®eld. The resulting maps for each ®eld were marked to show all horizontal, vertical and diagonal joins (contacts) between all high-valued quadrats, and eval-uated by the Krishna Iyer test for random distribution of high-value quadrats.

Aggregated population patterns were found for both fungal groups in both ®elds (Table 1). Lloyd's index of

patchiness values for A. ¯avus group in ®elds A and B

were 2.96 and 2.32. Lloyd's index of patchiness values for

A. niger group in ®elds A and B were 6.55 and 3.19. A Lloyd's index of patchiness value of 1.0 represents a random pattern (Pielou, 1977), with increasing values indi-cating increased degrees of aggregation.

For rankedA. nigergroup population densities in ®eld A,

there was strong evidence for similar population densities occurring in quadrats (samples) of close proximity (Table

1). ForA. nigergroup in ®eld B, for ranked data (Table 1),

and for unranked population density data [Moran's Iˆ

0:135; with a z-statistic of 1.87 …Pˆ0:0307†], there was

strong and marginal evidence, respectively, for quadrats in

close proximity having similar values. ForA. ¯avusgroup

ranked data in ®eld A, there was marginal evidence for quadrats in close proximity having similar values (Table

1). For A. ¯avus group ranked data in ®eld B, there was

very marginal evidence for quadrats in close proximity

having similar values (Table 1). For A. ¯avus group in

both ®elds and A. niger group in ®eld A, using unranked

data, no evidence was found for clustering of quadrats with similar values. As indicated (Table 1), choice of scale may markedly affect the values obtained for neighboring

popula-tion densities and the results obtained for Moran'sI. Ranked

data may be less sensitive to scale effects. Ranked data were examined in these tests because of the large differences in population density among the neighboring higher

popula-tion density quadrats. ForA. nigergroup in ®elds A and B,

plots of Morisita's index versus numbers of quadrats in a cluster, down the crop rows, indicated a cluster size of one

to two quadrats (13±26 m2) and one to four quadrats (13±

52 m2), respectively. Across the crop rows, a cluster size of

one to two quadrats (13±26 m2) was indicated in both ®elds.

The same was found forA. ¯avusgroup in both ®elds, either

down or across the crop rows.

Mean population densities forA. ¯avusgroup in ®elds A

and B were 33.9 and 29.2 colony-forming units (cfu) per g soil, respectively. Population densities ranged from 0 (one quadrat only) to 283.8 cfu per g soil for ®eld A and from 2.5 to 216.8 cfu per g soil for ®eld B. Mean population densities

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for A. niger group in ®elds A and B were 318.8 and 301.6 cfu per g soil, respectively. Population densities ranged from 24.9 to 4788 cfu per g soil for ®eld A and from 35.5 to 2656 cfu per g soil for ®eld B. Repeat analysis of random soil samples gave similar results.

Box plots of the lattices in ®elds A and B for A. ¯avus

group andA. nigergroup population densities are shown in

Fig. 1. Population densities ofA. ¯avusgroup andA. niger

group are shown using a gray scale covering the range of population densities found in the ®eld. Using the approach recommended by Pielou (1977), the null hypothesis of

random mingling of high-population density quadrats was

not rejected…P.0:05†in either ®eld A or B for theA. ¯avus

group by the Krishna Iyer (1949) test (Table 1). However, the null hypothesis of random mingling of high-population

density quadrats ofA. nigergroup was rejected…P,0:05†

for both ®elds. All high-density quadrats ofA. nigergroup

in ®eld A were clustered into one large patch, measuring

316 m2, where the patch is the sum of the areas of all joined

high population density quadrats. Similarly, all high-popu-lation-density quadrats, except one, in ®eld B were

aggre-gated into one large patch measuring 303 m2.

G.J. Grif®n et al. / Soil Biology & Biochemistry 33 (2001) 253±257

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Based on Lloyd's index of patchiness values, the results

indicate thatA. ¯avusgroup had a moderate level of

aggre-gation. Many soil-borne fungi examined previously had Lloyd's index of patchiness values of less than 2, indicative of low aggregation (Grif®n and Baker, 1991). Values greater

than 5, as found here forA. nigergroup in ®eld A, have not

been commonly reported. The spatial scale we used falls about mid-way in the table (for grain or quadrat size) given by Campbell and Bensen (1994) for 44 previous studies. Orum et al. (1997) recently pointed out the need

to determine the spatial pattern ofA. ¯avusin adjacent ®elds

in order to understand the factors that determine the spatial structure within and between ®elds. In the present study, the two ®elds were separated by about 300 m of woodland. Both ®elds had similar, moderately aggregated spatial patterns

and mean population densities ofA. ¯avusgroup.

The results obtained for Moran'sIstatistic, based on ranked

data, provided evidence that similar population densities ofA.

nigergroup tended to occur in close proximity in both ®elds. Similar ®ndings were found for unranked population density

data from ®eld B. The clump size range forA. nigergroup, 13±

52 m2,was found along the rows. Since this clump size was not

found across the rows, cultivation of the ®eld may have contributed to the movement of crop debris or fungal propa-gules in the ®eld and the orientation of clumps. Rarely has clump size in a ®eld been estimated for a soil-borne fungus (Mihail and Alcorn, 1987). The results of the Krishna Iyer test

indicated that the quadrats ofA. nigergroup, having greater

than the median population density, were not randomly distributed in each ®eld. Patch size estimates of 316 and

303 m2were relatively large.A. nigergroup is recognized as

a common competitor ofA. ¯avus group (Ashworth et al.,

1965; Joffe, 1969; Diener et al., 1987), and, like A. ¯avus

group, is favored by higher temperatures. However,A. niger

group may not be as competitive asA. ¯avusgroup at very low

water potentials (Diener et al., 1987). Conversely, somewhat moister areas or sites may favor the development of

high-population densities of A. nigerin both ®elds. Low, moist

areas were not apparent in either ®eld, but slight differences in topography may be dif®cult to detect. The texture of all soil samples appeared similar. Generally, areas in the ®elds with highA. niger group population densities did not have high

population densities of A ¯avus group. The high A. niger

group population densities found here may be a result of moist periods that occurred during crop debris decomposition.

Overall, the population densities found forA. ¯avusgroup

were higher than found previously in most Virginia peanut ®eld soils (Grif®n and Garren 1974; Grif®n et al., 1975; 1981), in which a¯atoxin occurrence is uncommon. These

higher population densities of A. ¯avus group, along with

the moderate aggregation found for this group, may be related, in part, to the occurrence of a¯atoxin in these two ®elds. Moderate aggregation could lead to patches in the ®eld with high population densities (greater than 200 cfu per g soil). For management considerations, the mixing effects of plowing and disking the ®eld may help break-up

aggregates and distributeA. ¯avusgroup propagules over a

greater volume of soil, possibly diluting these higher p-opulation densities.

Acknowledgements

We thank J. Ashley for help in locating the study ®elds.

References

Ashworth, L.T., Schroeder, H.W., Langley, B.C., 1965. A¯atoxins: envir-onmental factors governing occurrence in Spanish peanuts. Science 148, 1228±1229.

Campbell, C.L., Bensen, D.M., 1994. Spatial aspects of the development of root disease epidemics. In: Campbell, C.L., Bensen, D.M. (Eds.). Epidemiology and Management of Root Disease Epidemics, Springer, Berlin, pp. 195±243.

Cliff, A.D., Ord, J.K., 1973. Spatial Autocorrelation, Pion Ltd, London (178 pp).

Cotty, P.T., Bayman, P., Egel, D.S., Elias, K.S., 1994. Agriculture, a¯atox-ins andAspergillus. In: Powell, K.S. (Ed.). The Genus Aspergillus, Plenum Press, New York, pp. 1±27.

Diener, U.L., Cole, R.J., Sanders, T.H., Payne, G.A., Lee, L.S., Klich, M.A., 1987. Epidemiology of a¯atoxin formation byAspergillus ¯avus. Annual Review of Phytopathology 25, 249±270.

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associated organisms in soil in relation to infectious inoculum. In: Katan, J., DeVay, J.E. (Eds.). Soil Solarization, CRC Press, Boca Raton, FL, pp. 3±21.

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Grif®n, G.J., Garren, K.H., Taylor, J.D., 1981. In¯uence of crop rotation and minimum tillage on the population ofAspergillus ¯avusgroup in peanut ®eld soil. Plant Disease 65, 898±900.

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Orum, T.V., Bigelow, D.M., Nelson, M.R., Howell, D.R., Cotty, P.J., 1997. Spatial and temporal patterns ofAspergillus ¯avusstrain composition and propagule density in Yuma County, Arizona, soils. Plant Disease 81, 911±916.

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