DNA ISOLATION ON CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK: A FIRST STEP TOWARDS ITS ID CARD

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DNA ISOLATION ON CAPTIVE

SUMATRAN ELEPHANT IN ELEPHANT

TRAINING CENTER, WAY KAMBAS

NATIONAL PARK: A FIRST STEP

TOWARDS ITS ID CARD

Figure 1 . Isolated DNA genome in buf fer solution



Twenty eight individual elephants have been whole blood

sampled.



DNA was successfully separated from protein and other

component (Figure 1) and kept in -40

0

_{C. Qualitative test}

using agarose gel electrophoresis tec hnique.

Figure 2. Visualization of qualitative test for isolation of

DNA genome under UV transiluminator



In electrophoresis process 1% agarose gel was applied as

stationer phase, and Tris-acetate EDTA (TAE) as movement

phase.



DNA genome was separated in agarose gel based on electro

mobility of its negative c harge of DNA molecule in cer tain

distance.



Loading dye addition on DNA may clarify migration distance.



Sumatran elephant’s DNA genome was predicted more than

10kb. It is shown by its shor t migration distance from the

well.



Isolated DNA will be used for fur ther molecular analysis,

PCR-RAPD and DNA sequencing.



Twenty eight whole blood samples have been successfully

prepared for isolation of DNA . DNA samples were available

for fur ther individual genetic ID building, and kept in -20

o

_C.

Our high gratitude to Directorate of Researc h and Community

Ser vices, Higher Education Indonesia for researc h grant, Way

Kambas National Park for the scientific collaboration and

Veterinar y Bureau Lampung Province for Biomolecular analysis

works. Our thanks to Dr. Mikael Jazdzyk for literature suppor ts.