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molecular characterization of ifn t expressed in buffalo embryonic

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Academic year: 2017

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Fig. 1. RNA on 1.5% agarose gel revealing two distinct bands- 28s RNA and 18s RNA (A).PCR amplified product of BuIFN-T (694 bp) (B)
Fig. 2. PCR amplicolonies for both isoforms from transformed top 10 cells: showing three positive clones each for BuIFN-T1 and BuIFN- T8 isoform (B)
Fig. 3. SDS PAGE analyses of expressed and puriIPTG: lane M- Marker, lane 1 and 6- Uninduced (control), lane 2 and 3- BuIFN-T1a2 pellet and soluble fraction, respectively, lane 4 and 5- BuIFN-T8 soluble and pellet fraction,respectively) (B)

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