! " #$ % &' !&(#
ﻪﻧاﺪﮕﻧر و ناﺮﻳا هﺎﻴﺳ يﺎﭼ هرﺎﺼﻋ ﺮﺛا
نآ زا هﺪﺷ جاﺮﺨﺘﺳا ﻦﻴﺠﻴﺑورﺎﺌﺗ يﺎﻫ
يرﻮﺳ شﻮﻣ ﻚﻳرﺎﺑ هدور يرﻮﺒﻋ نﺎﻣز يور
يﺮﻔﻌﺟ شﻮﻧﺎﻴﻛ ) ، ﺮﺘﻛد ﺐﻳﺮﻏ رﺎﻳﺮﻬﺷ هداز 2 ، ﺮﺘﻛد ﻲﻬﻴﻘﻓ ﻪﻳﺪﻬﻣ * 3 , ﺮﺘﻛد ﻀﺗﺮﻣﺪﻴﺳ نﺎﻴﻤﻳﺮﻛ ﻲ 4 ، هﺰﻤﺣ ﺪﻴﺠﻣ ﻮﻟ 5 ، ﺮﺘﻛد زروﺎﺸﻛ رﻮﺼﻨﻣ 6 ﻪﺻﻼﺧ ﻪــﻣﺪﻘﻣ : !
(Camellia sinensis var sinensis) "#" $%& ' ( )* +%,-) !/01 ' ( 3 +0 4 -5 6 $0 . شور : !/01 8 3 0 ' 9 )* 8 . " 0 5 #"" 3 +0 4 ) : ;<= >" ( ? . ﻪﺘﻓﺎﻳ ﺎﻫ : 0 @A 4 B' @C BD- E0 F%< 0 >0 3 +0 ) GH / G P< .( !/01 JAC K" ' D" " 0 @C BD- ,' 0 >0 @A "#" ! L 3 +0 4 B' 0 ' ) GH / G P< .( ﻪﺠﻴﺘﻧ ﺮـﻴﮔ ي : M 0 K" $ F" 0 ! "#" $ ( ' ," $C @NOP $P0 ,0 Q 4 . هژاو يﺪﻴﻠﻛ يﺎﻫ : R 01 / ! ' , 4 0 + , 3 ! % * +, -./ 0 1 2 3 45 6 , 531 8 4 6 9 29 : : 9: :;< = % 3.: >:3.95? :8 :2+; 29: 9 .@+A B, C % 1 8 3 45 6 , 9 29 , 9 ;< , # % 1 8 . 3 45 6 , 9: :;< D % ? 8 – 1 3 > , 29 , ;< F % 1 8 3 45 6 , 9 29 , 9: : .:2;? G 2:/ – : ;< * لوﺆﺴﻣ هﺪﻨﺴﻳﻮﻧ : 64531 8 , 9 29 , ;< 9 , • >.95 HI J0 : faghihi2002@yahoo.com ﻪﻟﺎﻘﻣ ﺖﻓﺎﻳرد : $F / $ / !&(# ﻪﻟﺎﻘﻣ ﺖﻓﺎﻳرد هﺪﺷ حﻼﺻا : $D / !! / !&(# ﻪﻟﺎﻘﻣ شﺮﻳﺬﭘ : $D / !! / !&(# وﮋﭘ ﺔﻟﺎﻘﻣ ﻲﺸﻫ
ﺪﻘﻣ ﻪﻣ T & U( V& $"" ! WX ) Y .( $Z% [" 0 5
sinensis(L.) O. Kuntze (Theaceae) camellia ) ( Sinensis R Lasiocalyx Assamica ) \ ( ] Sinensis $ ) H .( 3 M 0 ^" (Black tea) R _, (Oolong tea) D4 (Green tea) X ) H` .( ^" ! @? $%& @4 B> D W"( Z Z' D4 0 Z' [/" $ . WX $- J aG % 0 Jc R \d % D4 Jc 0 \ % Jc 0 _, ) d .( $%& @4 "#" 0 $ZD"( B> 3* [ $' $01 0 !X [Thearubigins (TRs)] !O-1 [Theaflavins (TFs)] J4 $ " . ( JZ 0 @eef , D4 gP " $"( @ ,' > $" "h i $ ( ) Hj .( ( JZ 0 @eef D" " ? @ ,' ( $"( @ kZ' "> $ "#" !O-1 ' $01 !X ( 0 ) HG ( 0 ? @ !,kZ' ) a ( h R " P [ ) ` .( , P 35 l> 0 @ ? @ $C [/" mef lP ) H\ ( n "#" 01 ' !/ $C" $0W Z0 kZ' $5 '
(inflammatory bowel disease)
) HH .( # @eef [/" @ ) Assamica ( " @C ! $ B' BD- o4 " @C ! $ ( 4 ;W kZ' $5 $C @NOP ? ) p , q .( $/"( ^" Sinensis D ! $%& @4 Z "#" +%,-) HY ( ! 0 $0 ! !,kZ' !/01 $%A "#" ' 3 + @C P& . شور ﺳرﺮﺑ ﻲ هﺎﻴﺳ يﺎﭼ هرﺎﺼﻋ ﻪﻴﻬﺗ : W 0 % HGG Camellia sinensis sinensis
D @eef $ ? . o 0 j / HR sR j / ` t [5 HGG $% U( * X e V& j 0 ;%f e6 TA ( ;%f 0 00 o 0 j / H %R s %R j / ` % t % -5 F" " ) s .( F%< u- ' C ° sa 0 5 _" % v @A 0 in vivo 3 0 5 . ﻪﻧاﺪﮕﻧر جاﺮﺨﺘﺳا هﺎﻴﺳ يﺎﭼ زا ﻦﻴﺠﻴﺑورﺎﺌﺗ يﺎﻫ : "#" ' )* 3 0 mef ! @4 8 9 X 8 ) j .( 0 HG [5 sGG $% * X D" U( HG 0 e6 TA JAC ;%f V: H\G $% ;%f 0 YG $% * " [-% V& Hj c C 0 " c c e6 w5 Jc - . $ % !1- C J @4 X !"D5 . c [-% ? 0 # 0 JZ !Z' V: . * " JAC $0( N V: 0 ( `G $% J -i @ V& Hj e6 c c HG c C 0 " e6 c - w5 J . C $( -!O-1 X ' . c # 0 ! !O-1 " X $0( - ' . $0( - V: * " ( 0 `G $% -i ;"0 . V& Hj c c e6 HG C 0 e6 w5 c . c 0 D" JZ ! !/01 " X $(- ' . JC [Z ;OC l - *4 # % 0 ' R ,P0 x6 " X D-. !/01 $Z gP ZP )* ' 3 % 0 xh ' B?,0- $y $/,
(UV-Visible spectrophotometry) Z/" e" ;D" WX 0 -56 $0 $c [X ! 3D5 ' "#" 0 8X $%46 e0 ' ) H` , t .( ﻲﻫﺎﮕﺸﻳﺎﻣزآ تﺎﻧاﻮﻴﺣ : 3 ) Balb/c ( " V,X $" f \G sG ? [5 . @"C 5 ' z $ C D- 5 "P v #" $ W#" W $cD& [% " Hd B& " C 0 B ( ^ ) U( 0 $ 0 w< ( , 6 . هزاﺪﻧا يرﻮﺒﻋ نﺎﻣز يﺮﻴﮔ (Transit time) : " 0 5 #"" 4 ) >": ; ( 0 3
charcoal propulsion test
) {q , p ( ? . W 0 ; >": H\ 0 ; [5 \ |ZA [5 }%* $0 HsG $% * e U( . ; " ;, 5 0 ! ) gr HGG ml/ j / H ( L 5 _" v C . V& sG e6 ; #"" ) gr HGG ml/ j / H ( C 0 V& sG C e6 lc 0 V& D 0 [c %& Jf + RC 0 + ;h J 0 $h -> , 0 A o>C GIT% (Gastrointestinal Transit%) 4 4 . 5 ' !/01 0 $ ' )* R F%< 0 ' j / HR sR j / ` t [5 HGG $% W D 0 gr HGG ml/ j / H C ° sa 3 ' . ;%f !/01 0 ' sG R `G R `j R YG $% [5 0 0 W 3 0 [5% ' D gr HGG ml/ j / H " C . V& sG ; #"" e6 gr HGG ml/ j / H 3 0 sG C e6 X ( + ;h J 0 #"" $h -> D + , 0 GIT% 4 . يرﺎﻣآ ﻲﺳرﺮﺑ : ( D"( 0 [" ' D-SPSS ( ( ANOVA Zc > % $ tukey ? " @A 0 K Mean±SE 3D5 ) GH / G P< ( 0 $, ~ , -5 F" . ﺞﻳﺎﺘﻧ A ;, 5 GIT \ / jY % 4 5 ' ( B 0 F%< ' j / HR sR j / ` t [5 HGG $% A o 0 GIT Y / YG % R \ / aY % R dH % ja % 4 ) Z" H .( رادﻮﻤﻧ 1 : ./0 1 2 3 45 * +@ MN./ O.+, 8 ? P! / P P< SE) ± Mean ( $ ' F%< ' ml HGG / gr s ml HGG gr/ j / ` $, BD- ) GH / G P< ( 5 0 4>" + @C / ;, F%< " ml HGG / gr t , $ + @C / " c . 5 ' ,, -!/01 ' ) (TRs 0 sG R `G R `j YG $% 0 [5%' 0 [5 A C GIT o 0 Hj / YG % R aH %R a` % YH % 4 . ' 0 + @C `G `j $% [5 ) (TRs 0 C 0 [5% ' $, " ;, 5 0 >e ) GH / P< ( G YG $% $, [5 " ' + @C ) Z" \ .( 10 20 30 40 50 60 70 80 90 100 O.+, %!/D * * & % #/D R S HTU 6 5 B V 6 ) 2A ( Y % 5
60 HTU ?Z< [ (mg/kg) 8 دﻮﻤﻧ را 2 : 89: ./0 3 45 * +@ MN./ O.+, 8 ? \) / \ P< (Mean±SE ) ﺚﺤﺑ $ ( 0 K" 0 X 0 ?5 !, ! Camellia sinensis var sinensis @C BD- E0 F%< 0 >0 @A 0 3 +0 $ . /"( 5 ( 0 K" ' ,, -/01 ! ' D" " +0 @C BD- ,' "#" ! L 0 >0 @A 0 ' , ! " ^Z/ K " Be" ,' "#" ' 01 !/ X $ 0 . 0 !-5 F" F%< T T ' ) s j / ` [5 HGG $% ( $ J + , 0 +,& ) lF, ,' @C ,, 35 ( 0 Z . ( 0 K" 0 X 0 B'& L [/" Chaudhuri @ +,& Camellia sinensis var Assamica
> 5 mh, ) ` ( @C 35 " ,"Z' W 'Z>"" Be" ,' NO !"O5& D" > ' , l v BD- + +0 @C R X 0 0 @4 X 0 ) J !"D5 "#" ' $%& ' ( ' $ ^" BD- 0 '> X !/01 $" @C ' >" JZf . Z 0 X 0 4 D 0 "#" ' Sinensis 0 >e Assamica F%< @C N0 ' ) j / ` [5 HGG $% ( 0 Sinensis 0 >e s [5 HGG $% 0 ) Assamica ( $ J06 X ) Z" H .( M 0 K" $ FCO ( 0 0 F%< BD- 0 5 t [5 HGG $% ) t (% @C $Z" . !/01 !,kZ' ' YG $% $Z" ' @C $D- [5 5 . & BD- @NC v @C + Z' ;W ," ' & l> mh y @C B' o4 ) s .( ! $ /" 5 5 ^" 0 >0 l> D- ' v $ + B' BD- o4 " @C 35 F%< 4 T ' ' >" $- Assamica Sinensis BD- $ @C . ! M 0 [/" mef 0 $ -5 /" $C" Z" A 4 kZ' $5 ' . 0 10 20 30 40 50 60 70 ;< 100 ;, 30 40 45 B V 6 ) 2A ( 80
Summary
Effect of Iranian Black Tea Extract and Its Isolated Thearubigins on Intestinal Transit Time in Mice
Jafari K, M.Sc.,1 Gharibzadeh Sh, M.D., Ph.D.,2 Faghihi M, Ph.D.,3 Karimian SM, Ph.D.,4 Hamzehloo M, M.Sc.5 and
Keshavarz M, Ph.D.6
1. Master of Science in Physiology, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Assistant Professor, Department of Bioelectrics, School of Medical Engineering, Amirkabir University of Technology, Tehran, Iran 3. Associate Professor, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran Iran 4. Professor, Department of Physiology, School of Medicine, Tehran University Of Medical Sciences, Tehran, Iran 5. Lecturer, Department of Physical Chemistry, School of Science, Tehran University, Tehran, Iran 6. Associate Professor of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Introduction: In this study, the effects of black tea extract of Iranian tea plant (Camellia sinensis var
sinensis) and its polyphenolic pigment thearubigins were investigated on mice intestinal transit time.
Method: Thearubigins were isolated by liquid-liquid fraction method. The effects of black tea extract and
isolated thearubigins on intestinal propulsion were measured by charcoal propulsion test.
Results: According to the obtained results, black tea extract increases intestinal motility and conesquently
reduces transit time in a concentration dependent manner (P<0.01). Thearubigins, also, increase intestinal motility and decrease transit time dose dependently (P<0.01).
Conclusion: Based upon these results, Iranian black tea extract and its major polyphenolic pigment,
thearubigins, could be useful in the treatment of some gastrointestinal motility disorders.
Key words: Iranian black tea extract, Thearubigins, Mice, Intestinal transit time.
Journal of Kerman University of Medical Sciences, 2006; 13(1): 37-42
ﻊﺑﺎﻨﻣ ) . NU ; : +38 * 6R , . ^ G 2/ _, R 6 6R V 6V ;` GV , V +1 -3 .I )Ca= , " ) .
2. Barua DN: Science and practice in tea culture. Calcutta, Tea Research Association, 1989; PP9-11.
3. Besra SE, Gomes A, Ganguly DK, Vedasiromoni
JR. Antidiarrhoeal activity of hot water extract of
black tea (Camellia sinensis) Phytother Res
2003; 17(4): 380-4.
4. Chaudhuri L, Basu S, Seth P, Chaudhuri T, Bersa
SE, Vedasiromoni JR, et al. Prokinetic effect of black tea on gastrointestinal motility. Life Sci
2000; 66(9): 847-54.
5. Chen Ch, Ho Ch. Antioxidant properties of
polyphenols extracted from green and black teas.
Journal of food lipids 1995; 2: 35-46.
6. Gomes A, Das M, Vedasiromoni JR, Ganguly
DK. Proconvulsive effect of tea (Camellia sinensis) in mice.Phytother Res 1999; 13(5): 376-9.
7. Gomes A, Vedasiromoni JR, Das M, Sharma
RM, Ganguly DK. Anti-hyperglycemic effect of
black tea (Camellia sinensis) in rat. J
Ethnopharmacol 1995; 45(3): 223-6.
8. Gupta S, Chaudhuri T, Ganguly DK, Giri AK.
Anticlastogenic effect of black tea (world blend) and its two active polyphenols theaflavins and thearubigins in vivo in Swiss albino mice. Life Sci 2001; 69(23): 735-44.
9. Haslam E. Thoughts on thearubigins.
Phytochemistry 2003; 64(1): 61-73.
10. Leung LK, Su Y, Chen R, Zhang Z, Huang Y,
Chen Zy. Theaflavins in black tea and cathechins in green tea are equally effective antioxidants. J Nutr 2001; 131(9): 2248-51.
11. Maity S, Ukil A, Karmakar S, Datta N,
Chaudhuri T, Vedasiromoni JR, et al.
ameliorates mucosal injury in trinitrobenzen sulfonic acid-induced colitis. Eur J Pharmacol
2003; 470(1-2): 103-12.
12. Maity S, Vedasiromoni JR, Chahdhuri L,
Ganguly DK. Role of reduced Glutathione and nitric oxide in the black tea extract mediated protection against ulcerogen-induced changes in motility and gastric emptying in rats. JPN J Pharmacol 2001; 85(4): 358-64.
13. Nagakura Y, Naitoh Y, Kamato T, Yamano M,
Miyata K. Compounds possessing 5-HT3
receptor antagonistic activity inhibit intestinal propulsion in mice. Eur J Pharmacol 1996; 311(1): 62-72.
14. Peterson J, Dwyer J, Jacques P, Rand W, Prior R,
Chui K. Tea variety and brewing techniques
influence flavenoid content of black tea. Journal of Food composition and analysis 2004; 17: 397-405.
15. Vinson J.A, Dabbagh Y. Tea phenols:
antioxidant effectiveness of teas components, tea fractions and their binding with lipoproteins.
Nutrition Research 1998; 18(6): 1067-75.
16. Vinson J.A, Dabbagh Y. Tea phenols:
antioxidant effectiveness of teas components, tea fractions and their binding with lipoproteins.
Nutrition Research 1998; 18(6): 1067-75.
17. Wilson KC, Clifford MN: Tea cultivation to
consumption. 1st ed., Chapman & Hall, 1992; P414.
