릤䨸䨸릤䨸산산산릤산
릤䬬ꮠ갬산䨸 릤산 鮼ઈퟰઈὨ뀨牐牐Ὠ뀨뀨牐 魤牨산ꮠ산릤䬬䬬ɬ산ꮠ䬬牨산䨸ퟰ䀸산 牨
EFFECT OF NON IONIC SURFACTANT ADDITION TO CELLULASE PERFORMANCE
IN HIGH-SUBSTRATE-LOADING-HYDROLYSIS
OF PALM OIL EFB AND WATER-HYACINTH
Teuku Beuna Bardant1.*, Sudiyarmanto1, Haznan Abimanyu1, and Aisha Kania Hanum2
1
Research Center for Chemistry, Indonesian Institute of Science, Komplek PUSPIPTEK Serpong Tangerang Banten 15413
2
Department of Chemistry, Faculty of Science, University of Diponegoro, Jl. Prof. Soedharto Kampus Tembalang Semarang
Received October 21, 2012; Accepted February 7, 2013
ABSTRACT
Enzymatic hydrolysis with high substrate loading of palm oil (Elaeis guineensis empty fruit bunch (EFB) and
water-hyacinth (Eichhornia crassipes) were investigated as a prior part of ethanol production from lignocelluloses. Commercial surfactant Span 85 and Tween 20 were used as cellulase performance enhancer in hydrolysis process
with substrate loading above 20% (w/w). Cellulase performances were compared based on hydrolysis conversion.
Hydrolysis conversions of EFB using cellulase with concentration 10 and 15 FPU/g-substrate was 38.55% and
88.80% respectively. Addition 2% (v/v) of Tween 20 to EFB hydrolysis reaction with cellulase concentration
10 FPU/g-substrate gave the conversion 87.30%. This addition enhance the cellulase performance up to 226.5% or
similar with the performance of cellulase 15 FPU/g substrate. Addition 2% (v/v) of Span 85 to the similar reaction only
enhances cellulase performance to 174.7%. Hydrolysis conversion of boiling-pretreated water-hyacinth and autoclave-pretreated water-hyacinth using cellulase 15 FPU/g-substrate was 45.84% and 52.29% respectively.
Addition 2% (v/v) of Tween 20 and Span 85 to boiling-pretreated water-hyacinth hydrolysis with cellulase
concentration 15 FPU/g-substrate enhance cellulase performance of 128.9% and 153.5% respectively. Addition
1% (v/v) of Tween 20 and Span 85 to the similar reaction with cellulase concentration 10 FPU/g-substrate gave
conversions 51.00% and 53.79% respectively, or similar with conversion of autoclave-pretreated water-hyacinth hydrolysis with 15 FPU/g-substrate.
Keywordscellulose enzymatic hydrolysis; Tween 20; Span 85; bioethanol lignocellulose
ABSTRAK
Proses hidroliss enzimatik dengan beban substrat tinggi menggunakan Tandan Kosong Kelapa Sawit (TKKS) dan eceng gondok telah dikaji sebagai tahap awal proses produksi etanol dari lignoselulosa. Surfaktan komersial Span 85 dan Tween 20 digunakan sebagai pemacu kinerja selulase dalam proses hidrolisis dengan beban substrat
di atas 20% (w/w) ini dan kinerja diukur dari konversinya. Konversi hidrolisis pulp TKKS dengan konsentrasi selulase
10 dan 15 FPU/g-substrat berturut turut adalah 38,55% dan 88,80%. Penambahan 2% (v/v) Tween 20 pada hidrolisis
TKKS dengan selulase 10 FPU/g-substrat memberikan konversi 87,30%. Artinya penambahan ini memacu kinerja
selulase hingga 226,5% lebih tinggi atau setara dengan kinerja selulase 15 FPU/g-substrat. Penambahan 2% (v/v)
Span 85 hanya memacu kinerja selulase hingga 174,7% lebih tinggi. Konversi hidrolisis eceng gondok yang sebelumnya didihkan pada tekanan ruang (1 atm) dan pada tekanan autoclave (2 atm) menggunakan selulase
15 FPU/g-substrat berturut turut adalah 45,84% dan 52,29%. Penambahan 2% (v/v) Tween 20 dan Span 85 pada
hidrolisis eceng gondok yang sebelumnya didihkan pada tekanan ruang (1 atm) memacu kinerja selulase berturut
turut 128,9% dan 153,5%. Penambahan 1% (v/v) Tween 20 dan Span 85 pada reaksi berbahan baku sama dan
selulase 10 FPU/g-substrat berturut turut adalah 51,00% dan 53,79% atau setara dengan konversi hidrolisis eceng gondok yang sebelumnya didihkan pada tekanan autoclave (2 atm) menggunakan selulase 15 FPU/g-substrat.
Kata Kuncihidrolisis enzimatik; selulosa; Tween 20; Span 85; etanol lignoselulosa
INTRODUCTION
쀔ꮠꮠ산ꮠ ꮠ갬릤䨸䬬릤䬬 산䬬 릤ꮠ산 산䘐 牨산릤ꮠ산䬬ꮠꮠઈ릤릤갬䀸릤䬬䬬릤䬬 산䬬릤릤䬬䨸ꮠ릤 릤 릤䬬ꮠ릤䀸 魤䀸牨산ꮠ 䀸䀸䬬ꮠ䬬 릤䨸䬬릤 ꮠ
릤䨸䨸릤䨸산산산릤산 릤䀸牨산ꮠ릤䬬ꮠ 산牨ꮠElaeis guineensis 릤牨䀸 䨸ꮠ䨸 산䘐산릤ઈ 䀸산ꮠ (Eichhornia
crassipes). 산䘐牨산릤ꮠ산䬬䘐릤릤 䬬릤䨸릤
릤ꮠ산䨸산릤
릤산ꮠ갬䘐 산릤 릤ꮠ산䬬ꮠ릤䬬릤ꮠ릤䬬
Eichhornia crassipes E. crassipes 산䬬 산䨸䬬릤
릤牨䬬ꮠ산릤䬬산ꮠ릤䬬 䨸갬 ઈ䨸 릤䘐ꮠ䬬 릤 릤牨䬬릤 ꮠꮠ릤산산䬬릤䬬 䨸ꮠ릤䬬산 䨸산䬬 牨산䨸산산䘐산䬬릤䘐산릤䬬ꮠ䬬䬬䬬ꮠ릤 산 릤 산릤䬬릤ꮠ牨산䬬䬬 E. crassipes牨ꮠ갬 릤 䨸䬬릤릤릤 䨸 릤ꮠ산䬬ꮠ䬬산ꮠꮠ산䨸䬬릤 E.
crassipesꮠ牨산䬬䬬 산䬬릤릤ꮠ牨ꮠ릤䬬ꮠ산갬릤갬산ꮠ
牨䬬ꮠ갬산䬬왌㮴
EXPERIMENTAL SECTION
Materials
Hydrolysis experiments
릤릤산ꮠ䬬䘐릤릤ꮠꮠꮠ산릤䀸릤산ꮠ갬릤䀸牨릤 䬬䨸ꮠꮠퟰ牨魤릤牨릤䀸릤䀸牨ꮠ ꮠ갬릤䨸산䬬릤산
릤䨸䨸릤䨸산산산릤산
Table 1.⟴䨸 산산 릤牨ꮠ산䬬䨸䨸릤
Fig 1.魤䨸 䀸䀸䬬ꮠ䬬릤䬬ꮠ
Fig 2.릤䬬ꮠ릤산䨸䘐산릤릤䨸䬬릤릤䨸산䬬릤ꮠ릤산ꮠ릤䨸산䬬릤릤산ꮠ산ꮠ산䨸䬬릤䀸ꮠ릤릤䬬ꮠ릤
ꮠ릤산ꮠ릤䘐릤릤릤䨸䬬릤 산ꮠ산릤䨸산䬬릤릤릤䬬ꮠ릤릤䨸䬬릤ઈ릤䨸산䬬릤ꮠ릤산ꮠ산䨸䬬릤䀸릤䨸산䬬릤 릤산갬릤산릤ꮠ릤䨸䬬릤䀸䬬산ꮠ릤䬬䨸䨸릤﵌산산산릤산䨸䬬릤䀸ꮠ릤릤䬬ꮠ릤ꮠ릤산ꮠ 릤䘐릤릤 䀸 ꮠ산 릤䨸산䬬릤䘐ꮠ ꮠ갬ꮠ魤ꮠ䬬䬬ઈ릤䬬䬬ઈ릤릤
릤䨸牨릤릤산 뀨牨⟴ꮠ䬬䨸䬬산릤 䀸䀸䬬ꮠ䬬 릤 릤ꮠ牨릤䬬 릤릤갬䨸䀸산ꮠ갬Ⳉ䘐䘐 릤 䬬릤릤䬬䨸䬬산릤 릤릤 릤ꮠ牨릤䬬䘐릤릤릤 牨릤ꮠ 牨릤牨릤산䨸릤 릤 䀸䀸䬬ꮠ䬬 䘐산䬬릤牨ꮠ산릤䀸 ꮠ릤ꮠ갬 릤牨ꮠ 䨸릤 䨸갬 ꮠ릤 산릤릤䬬ꮠ䘐산䬬산䨸산릤 牨 릤릤䬬䨸릤 䬬䨸갬산䘐 ꮠ 䘐산䬬牨릤산䬬䨸릤산䬬릤릤䨸ꮠ갬릤 䨸䬬ꮠ갬䨸 ⟴ 牨릤 산ꮠ갬⟴ퟰઈ牐ઈ ퟰ牐牐
릤릤䨸산䬬릤䘐산䬬산ꮠ릤ퟰ산ퟰ뀨쀔갬 䬬䨸䬬산릤ꮠ䀸䘐릤ꮠ갬 ⟴䨸 산산산ꮠꮠ䘐산䬬산ꮠ릤ퟰ
릤䨸䨸릤䨸산산산릤산
RESULT AND DISCUSSION
EFB Hydrolysis and Surfactant Effect
苨ꮠꮠ 릤릤릤䬬릤ꮠꮠ䬬䨸 산산䘐산䬬
Water-hyacinth Hydrolysis
릤䨸䨸릤䨸산산산릤산
⟴䨸䬬산릤䬬䨸䨸산 산갬릤䬬산䨸䬬릤䀸䬬䨸 산산䘐릤릤 릤ꮠ갬牨릤 산ꮠ산릤牨릤 산ꮠ䬬牨 ꮠ ꮠ䬬산䬬릤 䘐릤릤牨릤릤 릤䬬ꮠ릤䬬䨸䀸䘐릤릤 릤ɬ䨸ꮠ릤 릤 산ꮠꮠ갬 䘐 릤䬬䨸䨸산 산갬릤䬬산䘐 䀸 릤릤䘐 䬬䨸䨸릤牨산릤 릤䬬䨸䬬산릤릤산䬬ꮠ릤릤 䀸䀸릤䀸 릤䀸牨릤
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