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4t h Int ernat ional Conference on Biosciences and Biot echnology “ Advancing life sciences for healt h (ant i aging development in part icular) and food securit y”

TABLE OF CONTENT

Biru: fullpaper sudah masuk layout

Hijau: hanya abstrak, sudah masuk layout

ORAL PRESENTATION: FOOD SCIENCE AND TECHNOLOGY

Code Name Title

O-1-01 TRIARTI SARASWATI

Human Factors Consideration in Food Industry to Minimize Health Problems as Impact of Human-machine Interaction

O-1-02 (permintaan ybs)

NI WAYAN ARYA UTAMI

Feeding Yacon Tuber-containing Oligofructose Increases Intestinal Fermentation and Alters Microbial Community Structure in Rats

O-1-03 NANI PASARIBU THE STUDY OF STABILITY OF ANTIOXIDANT ACTIVITY IN BUTTERFLY PEA (CLITOREA TERNATEA) EXTRACT SYRUP O-1-05 IDA BAGUS PUTU

GUNADNYA

Effect of Initial Concentration of CO2 and Storage Temperature on the Characteristic of Packed Broccoli

O-1-06 NI PUTU EKA LELIQIA

CHARACTERISTICS OF MICROORGANISMS IN BLACK TEA BROTH (KOMBUCHA) IN BALI

ORAL PRESENTATION: BIODIVERSITY AND ENVIRONMENT

Code Name Title

O-2-01 Iryanti Eka Suprihatin Reducing water hardness using extract of ketapang (tropical betel nut) leaves

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O-2-03 KHALIDA KHAN Socio-ecological Landscape Change as a Preamble to Urban Watershed Rejuvenation, Kanshi of the Jhelum River Basin, Potohar Plateau,

Fertility Enhancing Effects of Gynura procumbens Methanolic Extract on Diabetic Induced Male Rats

O-3-02 (permintaan ybs)

ISSABELLINA D. TAMPUBOLON,

Detection of Jembrana Disease Virus Amplicon by Lateral Flow (Immuno) Assay

O-3-03 SYAHIDA AHMAD Anti-Neuroinflammatory Effects of Cardamonin, a Chalcone Derivative Isolated from Alpinia rafflesiana

O-3-04 I WAYAN DWISADA

PURNAMAYADI

In Vivo Antimalarial Activity of Ethanolic Extract of

Spondias pinnata (L.f) Kurz. Leaves

O-3-05 AFIONO AGUNG PRASETYO

Occult Hepatitis B Detected in Human Immunodeficiency Virus Patients in Moewardi General Hospital Surakarta

O-3-06 RASMAYA NIRURI A Pilot Project in Monitoring Serum Concentration Following Administration Amikacin Once Daily Dosing in Electrical Burn Patients at Sutomo Hospital

O-3-07 NARENDRA YOGA HENDARTA

Study Protocol: Development Multiplex Dipstick for Early Detection Human Immunodeficiency Virus -1 and Hepatitis B Virus Based on Combination of Multiplex Loop-Mediated Isothermal Amplification and Lateral Flow Dipstick

O-3-08 IRDHAWATI Voltammetric Behavior of the Transfer of Methyl Ephedrine Ion across the Water|Nitrobenzene Interface

O-3-09 NI PUTU ARIANTARI

Phytochemical Analysis and In Vitro Antimalarial Activity of Ethanolic Extract of Spondias pinnata (L.f) Kurz. Leaves

O-3-10 confirmd

RINI NOVIYANI Study on Prescribing and Antibiotic Dosage Accuracy in Ear, Nose and Throat Cases Prescriptions (Case Studies in Seven Pharmacies in Denpasar City Period January – June 2010)

O-3-11 Permintaan ybs

I MADE JAWI AQUEOUS EXTRACT OF BALINESE PURPLE SWEET POTATO

(Ipomoea batatas L.) PREVENTS OXIDATIVE STRESS AND DECREASES BLOOD INTERLEUKIN-1 IN HYPERCHOLESTEROLEMIC RABBITS

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ZUHROTUN NISA O-3-13 TENRI ASHARI

WANAHARI

A Comparative Study on Jembrana Disease Virus Detection Methods

O-3-14 KETUT SRI MARHAENI JULYASIH

Extract Ethanol Seaweeds Lowering Level of Serum HMG-CoA Reductase Enzyme of Wistar Rats Hypercholesterolemia

O-3-15 Confirmd

I N. Sujaya BIFIDOGENIC NATURE OF INDIGENOUS BALINESE ROOT CROPS

ORAL PRESENTATION: AGRICULTURE

Code Name Title

O-4-01 BAMBANGRAHAYU TRISNO

PUJIASTOMO

The Use of Chitino-Proteolytic Actinomycetes As Biological Control Agens For Root-Knot Nematodes Meloidogyne sp.

O-4-04 RINDANG DWIYANI Micropropagation of Vanda tricolor Lindl. var. suavis forma Bali Carrying KNOTTED1- LIKE Arabidopsis thaliana (KNAT1) Gene

O-4-06 MADE

PHARMAWATI

Ethyl Methanesulfonate Delayed Germination and Altered Seedling Morphology of Capsicum annuum L.

O-4-08 Confirmd

Yan Ramona IN VITRO ANTAGONISM OF BACTERIAL BIOCONTROL AGENTS ON SEVERAL FUNGAL PLANT PATHOGENS

O-4-09 BUDI PRAKOSO The Effect of Seed Treatment with Liquid Product of Shrimp Waste Fermentation on Chili

O-4-10 MARLINA Detection of toxR, tdh and trh Genes of Vibrio parahaemolyticus Obtained from White Shrimp (Penaeus indicus) Samples in Jambi, Indonesian Coastal Area

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No Name Title

P-1-01 NI W. WISANIYASA THE INFLUENCE OF SOLVENT TYPES AND pH ON THE ANTIOXIDANT ACTIVITY OF ANTHOCYANIN OF FLESH RED DRAGON FRUIT (Hylocereus costaricensis) EXTRACT

P-1-02 DIAH PUSPAWATI The Change of Antioxidant Activity of Tamarillo Fruit (Chyphomandra betacea S) after Processing Into Sauce

P-1-03 confirm d

Ida Ayu Rina Pratiwi Pudja

CHANGES OF TEXTURE AND RESPIRATION RATE OF SALAK FRUIT IN MODIFIED ATMOSPHERE USING POLYETHYLINE PLASTIC PACKAGING AT VARIOUS PERFORATION

P-1-04 (confirm ed ybs)

SRI MULYANI The Effect of Ratio Tamarind Pulp and Tamarind Leaves (Tamarindus indica L) and Cooking Time on The Antioxidant Activities

P-1-05 TIMUR INA Effect of CMC Concentration on Characteristic of Pineapple (Ananas comosus L.Merr) Juice P-1-06

confirmd

I KETUT SUTER1 Study on Storage Method of Instant Ledok

P-1-07 NI M. YUSA The Influence of Solvent Type and Ratio of Material With Solvent on Antioxidant Activity of Pulp Tamarillo Ekstract (Chyphomandra betacea Sendth).

P-1-8 BAMBANG ADMADI HARSOJUWONO

Determination of Potato Sweet Varietes As Diet Food Of Diabetes Mellitus Patients

P-1-9 confirmd

YAYA RUKAYADI* Antibacterial Activity of Fermented Pangium edule Reinw. Seeds Extract against Foodborne Pathogens

P-1-10 confirmd

YAYA RUKAYADI1 Shelf-Life Stability of Milk Chocolate Containing Inulin

P-1-11 NI NYOMAN PUSPAWATI

Survival of freeze-dried Lactobacillus rhamnosus R21 in the presence skim milk as protectant during storage

P-1-12 GUSTI AYU EKAWATI1 Extraction of Natural Colorant From Red Cabbage (Brassica oleracea var. capitata l. f. rubra (l.) thell) By Using Tartaric Acid

P-1-13 AGUNG ANAK MADE DEWI ANGGRENI

The Usage Of Beans Flour As Protein Source In Wet Noodle Processing

P-1-14 WIDNYANA YOGA Identification of Gel Forming Component (GFC) of Kacapiring Leaf ( Gardenia jasminoides Ellis)

P-1-15 Putu Ari Sandhi Wipradnyadewi

THE APPLICATION OF Rhizopus oligosporus MK 1 TO REDUCE AFB1 CONTAMINATION IN TEMPEH FERMENTATION

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WARDITIANI

TG Belawa Yadnya The Usage of Purple Sweet Potato (Ipomoea batatas) In Ration Fermented by Aspergillus niger For Improving Feed Conversion and Blood Lipid Profile of Bali Duck

Wayan Rai Widarta Extraction of Bioactive Compounds and Antioxidant Activity of The Local White, Red, and Black Rice Bran Extracts by Various Solvents

Siti Helmiyati Study Protocol: Effect of Iron Fortification of Fermented Milk with Local Probiotic Lactobacillus plantarum Dad 13 and Prebiotic Fructo Oligosaccharide on the Iron Content and Total Lactic Acid Bacteria During Storage

POSTER PRESENTATION: BIODIVERSITY AND ENVIRONMENT

Code Name Title

P-2-01 NI LUH SURIANI HEAVY METAL BIOREMEDIATION LEAD (Pb) TO AGRICULTURAL LAND ON THE EDGE OF LAKE BUYAN BALI WITH PLANT Sansevieria lorentii

P-2-02 Confirm d

ENIEK KRISWIYANTI, Alleles Variation of Coconut Accessions (Cocos nucifera L., Arecaceae) Based on Microsatellite DNA.

P-2-03 confirmd

I KETUT JUNITHA Genetic Diversity of Soroh Celagi (Pasek Catur Sanak Clan) Based on Y-Chromosomal Microsatelites DNA ,

P-2-04 confirmd

SUMIYATI Study of Socio-engineering the Subak System Development with Agroecotourism Oriented

P-2-05 I WAYAN SUSILA The Identification and Investigation of Parasitization Rate of Parasitoids of Hairy Caterpillar Lymantria marginata Wlk. (Lepidoptera: Lymantriidae) in Buleleng Bali

P-2-06 confirmd

IDA BAGUS WAYAN GUNAM

Biocatalytic desulfurization of dibenzothiophene by Pseudomonas sp. strain KWN5

P-2-07 confirmd

IDA AYU ASTARINI Induce Poliploidy of Lilium longiflorum Thunb. using Oryzalin Treatment

P-2-08 Ni Made Indri Hapsari Composting Acceleration of Kitchen W aste Using M icrobial Inoculant

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Code Name TITLE

P-3-01 Darukutni Use of Glucose Determination for Candida albicans β Glucans Examination P-3-02

confirmd

DEWA NYOMAN OKA THE IMPLEMENTATION OF MODULE-JIGSAW COOPERATIVE LEARNING STRATEGY, IMPROVING THE PREVENTIVE BEHAVIOR TOWARD DENGUE HEMORRHAGIC FEVER

P-3-3 confirmd

NI GUSTI AYU MANIK

ERMAYANTI

The Effect of Amargo Wood (Quassia amara Linn.) Infuse on the Mice (Mus musculus L.) Testical Weight and Its Recovery

P-3-4 confirmd

I GUSTI AYU SRI MAHENDRA DEWI

Classic Hodgkin’s Lymphoma Simulating Anaplastic Large Cell Lymphoma

P-3-5 confirmd

YULIA SARI Molecular Analysis of HBV Precore/Core Region of Hepatitis B Virus Isolated from Men Who Have Sex With Men in Surakarta

P-3-6 confirmd

RUBEN DHARMAWAN Fungal Infection in Human Immunodeficiency Virus Patients in Moewardi General Hospital Surakarta

P-3-7 confirmd

SUYATMI Apoptin Expression Plasmid For Expression of Apoptin in Mammalian Cells

P-3-8 confirmd

MARWOTO Mycobacterium Tuberculosis in Human Immunodeficiency Virus Patients’ Blood in Moewardi General Hospital Surakarta

P-3-9 confirmd

LELI SAPTAWATI Mycobacterium Tuberculosis Complex (MTC) and Nontuberculous Mycobacteria (NTM) in Human Immunodeficiency Virus Patients’ Blood in Moewardi General Hospital Surakarta

P-3-10 confirmd

HUDIYONO Molecular Analysis of HBV Pre-S/S Region of Hepatitis B Virus Isolated from Men Who Have Sex With Men in Surakarta

P-3-11 NI PUTU

ARYADNYANI

Extending Time of Kombucha Tea Fermentation Improves The Growth of Impediment Capasity of Bacteria Escherichia Coli ; The Producer of Extended Spectrum Beta Lactamases (ESBL) by In Vitro Method

P-3-12 NI PUTU SRIWIDYANI ROLE OF P53 IN CELLULAR AGING

P-3-13 confirmd

MADE AGUS HENDRAYANA

Identification of Vibrio cholera’s ctxA Gene by PCR Methode from Sea Water Around Bali Island

P-3-14 I MADE SUKADANA Antibacterial Terpenoid Compounds from Rhizome of Curcuma zedoaria (Berg). Roscoe

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P-3-16 confirmd

ANAK AGUNG AYU MIRAH ADI

Amplifications of Terminal Ends of Newcastle Disease Virus Genome by Rapid Amplification of Complementary Deoxyribonucleic Acid Ends

P-3-17 (confirm )

I NYOMAN SUARSANA Antioxidant Enzyme Activities In Various Tissue On Rat Under Hyperglycemia Condition

P-3-18 confirmd

I G K NYOMAN ARIJANA

Epigenetic Approach as Potential Anti-Aging Therapy of Andropause in the Future

P-3-19 confirmd

I WAYAN SUMARDIKA Antioxidative And Nephroprotective Activity Of Balinese Purple Sweet Potato (Ipomoea batatas L.) In Diabetic Rats

P-3-20 KOMANG ARDI WAHYUNINGSIH

TREATMENT OF TOPICAL ASTAXANTHIN GIVE A PROTECTIVE EFFECT ON COLLAGEN IN PREMATURE SKIN AGING INDUCED BY UV B IRRADIATION IN MICE (Mus musculus)

P-3-21 confirmd

IBN Putra Dwija Human Papilloma Virus Genotyping from Archive of Paraffin Block of Patient with CIN Diagnose

P-3-22 confirmd

K.A. Nocianitri1 Development of specific PCR-RAPD Method for detection of Lactobacillus sp F2

Confirm d

Ni Made Linawati The Role of Interleukin 22 in Lung Tuberculosis and Its Molecular Aspects

Confirm ed

IGA Dewi Ratnayanti THE ROLE OF p16INK4a IN CELLULAR SENESCENCE

Confirm d

IA Wahyuniari THE EXTRACTS OF CACAO BEANS (Theobroma cacao L.) INCREASE BLOOD SOD CONCENTRATION IN OXIDATIVE STRESS WHITE RAT (Rattus norvegicus)

Paramasari Dirgahayu

Coinfection of Toxoplasma gondii among HIV/HCV infected and uninfected people in Correctional

facility Indonesia

P-3-xx Agung Wiwiek Indrayani

The Study of Bcl-2 Expression of Red Fruit Oil Ethanol Extract on Breast Cancer Cell Line T47D

Confirm

d

Ni Made Adi Tarini DUPLEX PCR IS RAPID METHOD FOR DETECTION Legionella pneumophila and Legionella sp ON ENVIRONMENTAL WATER IN

Sri Budiarti Isolation and Characterization lytic Phage of Salmonella sp. FR38.

Made Sudarjana ADMINISTRATION OF ORAL ALPHA-LIPOIC ACID INHIBITS THE REDUCTION OF SKIN DERMAL COLLAGEN

EXPRESSION INDUCED BY UVB IRRADIATION IN BALB/C MICE

P-3-31 confirmd

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P-3-xx confirmd

N.W.Bogoriani SAPONINS APPLICATIONAS ANTI CHOLESTEROL

POSTER PRESENTATION: AGRICULTURE

Code Name TITLE

P-4-1 confirmd

I WAYAN ARNATA Production of Crude Enzyme Cellulases from Cassava Waste by Trichoderma viride

P-4-2 confirmd

NI MADE WARTINI THE INFLUENCE OF CURING TIME AND EXTRACTION TIMES ON THE YIELD AND CHARACTERISTICS ESSENTIAL OIL OF SANDALWOOD FRANGIPANI FLOWERS (Plumeria alba).

P-4-3 FACHIRAH ULFA Potential Of Plant Extracts As Growth Regulators: The Influence of Plant Extract to Growth of Cutting of Potato Seeding.

P-4-4 TUTIK KUSWINANTI, Detection of Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X (PVX) on Five Potato Varieties by Using of DAS-ELISA and RT-PCR Methods

P-4-5 confirmd

MADE RIA DEFIANI Media Composition Improved The Growth of New Leaves in Anthurium Plants

P-4-6 Confirm d

I G. L. O. CAKRA Urea-Lime Mixture as a Rumen Slow-Release Urea on In Vitro Rumen Fermentation and Microbial Protein Synthesis

P-4-7 Confirm d

IDA AYU RINA PRATIWI PUDJA

Technique " Pulsing" With Addition Of Sucrose And Antimicrobial To Change Of Texture and Freshness of Roses During Storage

P-4-8 YU JIN JUNG , KYUNG HEE HAN, HEE WAN KANG, ILL SUP NOU, KWON KYOO KANG

Soil Improvement Effects of Four Green Manure Crops in Greenhouse Cultivation

Effect of Green Manure Crop Extracts on Potato Common Scab (Streptomyces scabiei)

P-4-10 confirmd

G. A. M. KRISTINA DEWI

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P-4-11 I DEWA MADE PUTRA WIRATAMA1

The Variety Trial of Resistant Chili Peppers to Anthracnose

P-4-12 GUSTI NGURAH GEDE DHARMA PUTRA1

Monitoring of Imago of Spodoptera litura and Helicoverpa armigera Using Sex Pheromone Trap

P-4-13 I MADE SUKEWIJAYA The Misting Period Application on Postharvest Quality of Oyster Mushrooms (Pleurotus ostreatus) in Low Land Cultivation

P-4-14 Made Sritamin

Confirmation (Citrus Vein Phloem Degeneration) CVPD Infection Toward Orange Plants Varieties with PCR Technique and Total Protein Profile

P-4-15 confirmd

NI MADE INDRI HAPSARI

ARIHANTANA1

Composting Acceleration of Kitchen WasteUsing Microbial Inoculant

P-4-16 confirmd

Ketut Ayu Yuliadhi The Parasitism Level of Parasitoid Larvae Diadegma semiclausum Hellen (Hymenoptera:Ichneumonidae) on Plutella xylostella (Lepidoptera: Plutellidae) in Candikuning Village, Tabanan Regency, Bali, Indonesia

P-4-17 HILDA KARIM SCREENING AND CHARACTERIZATION OF MICROBIAL ANTAGONISTS FROM RHIZOSPHERE OF PASSION FRUITS (PASSIFLORA SPP) AS BIOCONTROL AGENTS OF FUSARIUM WILT

P-4-18 Ni Luh Yulianti DESIGN OF THE FLUIDIZED BED DRYER FOR CHILI SEED

P-4-19 Confirm ed

SUTARPA , I N.S1 The Effect of Waste From Purple Sweet Potato Syrup Production on the Performance, Lipid Blood Content, and Total Bacteria of the Digestive Tract of Kampong Chickens

P-4-xx IGA Arta Putra The Evaluation of Offering Cortisone Hormone and Ration Supplemented by Papaya (Carica papaya) Leaf Meal to the Performance of Duck on Growth Phase

P-4-25 confirmd

I. G. N. G. BIDURA THE IMPLEMENTATION OF Saccharomyces Spp.N-2 CULTURE (ISOLATION FROM TRADITIONAL YEAST CULTURE) FOR DECREASING ABDOMINAL FAT AND SERUM CHOLESTEROL CONTENTS OF BALI DUCKLING

P-4-26 confirmd

I GEDE RAI MAYA TEMAJA

First Report on Occurrence of Grapevine Leaf Roll-associated Virus Type 3 on Grapevine in Indonesia

P-4-27 Kumala Dewi The effect of Glucose and Light Quality on Vitamine E and

Chlorophyll content of Green Bean Sprout (Vigna radiata (L.) Wilcz.)

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CHANGES OF TEXTURE AND RESPIRATION RATE OF ZALACCA FRUIT IN MODIFIED ATMOSPHERE USING POLYETHYLINE PLASTIC PACKAGING AT VARIOUS PERFORATION

Ida Ayu Rina Pratiwi Pudja

Department of Agriculture Engineering, Faculty of Agricultural Technology, Udayana University

Email: dayu_rina@yahoo.co.id

ABSTRACT

The aim of this reaserch were to study changes of texture of Zalacca fruits from consequence of modified atmosphere packaging and to study of respiration rate of Zalacca fruit to preserve at modified atmosphere packaging.

The treatment of this reasearch was packaging with polyethyline plastic package or passive atmosphere with thickness 0.02 mm and perforation diameter hole is 0.6 cm. The various perforation is 0, 4, 8 and 12 hole. The storage time was set at 0, 2, 4, 6, 8, 10, 12 day in room temperature.

The result showed that the changes of texture of Zalacca fruit was increased during the storage time. The changes of texture rate was slowly at 8 hole polyethyline plastic perforation packaging. At the begining of the storage time, the respiration rate have been increased while in the midle have been decreased. Finally, at the end, the storage time have been increased. The respiration rate was slowly at 8 and 12 hole polyethyline plastic perforation packaging.

Keywords : Modified Atmosphere, Polyethyline plastic perforation packaging,

Zalacca Fruit

INTRODUCTION

There is inclination that consumer be apt to food product which are fresh, practical and high quality (Daugherty, 1990; Labuza and Breene, 1989; Myers, 1989). In last decade in the U.S.A., it was noted that consumption of fresh food product increase in 12%, frozen food product increase by 10%, but canning food product decrease by 10%. Therefore, Indonesia has good opportunity of exporting fruits and vegetables. In order to increase the export value of agro-industry, fruits and vegetables should be cared and handled very well.

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peeled off, and bruised. Microbe damage is caused by infection and microbe activity. Physiological damage occurs due to internal metabolism reaction resulting in putrefaction.

Zalacca (Salacca edulis, Reinw) is Indonesia native fruit. Production was high, reaching 662,546 tons in 1995 (Anon, 1995 in Agung et. al., 1999). The cultivation areas of zalacca in Indonesia are Bandung (Batujajar), Tasikmalaya (Manonjaya), Jogjakarta (Sleman), Malang, North Sumatera, South Sulawesi, Central Java (Ambarawa and Magelang) and Bali.

The increasing production and fulfillment of demand should be balanced with post harvest treatment. This should be considered becausezalacca as other fruits is easy to get damage. The negative environment factors along the storage cause of fast decreasing quality resulting in short storage time.

In order to increase the storage time and to avoid damage, delaying the mature is performed by decreasing oxygen absorption and release of CO2 of zalacca. This can be done with atmosphere modification.

Low oxygen and high CO2 in modified atmosphere give influence in decreasing respiration rate, ethylene

production rate, retard the maturation and others relate to mature such as soften of fruit, change of color, lose of sugar and retard the putrefaction (Brecht, 1980; Kader, 1980).

An appropriate post-harvest treatment of zalaccamay give result in added value of long storage time which means more profit for the farmer, seller and other parties.

AIMS

The aims of the research are

a. Studying changes of texture of fruit as result of storage in modified atmosphere.

b. Studying respiration speed of zalacca which are stored in modified atmosphere as function of O2.

METHODS

The research steps are preparation, respiration speed quantification, changes of the texture of fruit as result of storage in modified atmosphere, descriptive and graphical analyze.

A. Treatments

The treatments are polyethylene plastic cover with thickness of 0.6 cm (positive modified atmosphere) and storage time. The perforation of polyethylene plastic cover are 0 hole (without perforation), 4 holes, 12 holes and the storage time are 0, 2, 4, 6, 8, 10, 12 days. The samples treatment and one which is uncovered and used as the control group are stored in room temperature.

B. Respiration rates a. In tight close container

Close system is used in this experiment (Hangar et al., 1992 and Lee, 1997 in Song et al., 1992). Three samples are put in glass container/jarwith volume of 2.126 liters. Glass container is closed tightly (with glue/paint/vaseline to avoid leak).

In the middle of the lid, a hole cover with rubber is set to take the gas sample. This container is put in room temperature. Gas samples are collected every 2 days in 12 days. O2 and CO2 concentration are

detected with cosmotector. Type XP-314 cosmotector is used for O2 and XP318 for CO2. The relieved

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b. In polyethylene plastic

Three samples are put in a sealed 6.80 liters volume of polyethylene plastic. A hole cover with rubber is set in the bottom part of the polyethylene plastic to make easy for taking the gas sample. The edge of the hole is sealed with glue/paint/vaseline to avoid leak. And then is put in room temperature. Gas samples are collected every 2 days in 12 days. O2 and CO2 concentration are detected with cosmotector. Type XP-314

cosmotector is used for O2 and XP318 for CO2. The relieved volume in the container is also counted. These

treatments are repeated for three times.

c. Change of Texture

The probe with diameter of 0.52 mm of FT 327 model of Hand Penetrometer Effegi is used to test physically the stringent of Bali’s zalacca to get data of texture of Bali’s zalacca. A small area of skin of sample was peeled and then holds by one hand while the other hand pushed the sample with Hand Penetrometer Effegi. The number in kg/cm2 read from the tool shows the texture value of the fruit. The test was repeated for four times for each sample and it was done for some samples. The values were then calculated for the average number.

RESULT AND DISCUSSION

1. Changes of Texture

Texture is a decisive factor of fresh fruits. Changes of texture of Bali’s zalacca showed that there was a variation of texture of Bali’s zalacca in storage. The graph of texture changes can be seen in Figure 1.

Fig 1. The changes of texture of Bali zalacca fruits during the storage time

Figure 1 showed that the changes of texture level of control of Bali’s zalacca and all the Bali’s zalacca treated with different perforation of polyethylene plastic container decrease during the storage. The decrease intextureis caused by saturated decomposing propectin to pectin enzyme activity. The solid state rate is caused by the change of cell size and turgor. The reason of texture become lossis related with high level of respiration activity and transpiration of the fruit causing loss of liquid. Therefore, cell size and pressure of inside cell to the cell wall decrease (Suter, 1988).

5 5.5 6 6.5 7 7.5

0 2 4 6 8 10 12 14

Storage time (day) Texture (kg/cm2)

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2. Respiration Rate

The data of O2 respiration rate of Bali’s zalacca in storage is presented in Figure 2. Figure 2 showed

that in every treatment, respiration rate of O2 changed rapidly, then were going slowly, and finally in a

certain time changed rapidly until the last period of storage.

Figure 2shows that O2 respiration rates were increase in the beginning of storage, then decrease, and

increase again till the last period of storage. Decrease of respiration rate is caused by a decrease in the substrate for respiration. As addition, the O2 in the container is used by the fruits for respiration and

substrate oxidation. With limitation of O2 in the container causing the delay of chlorophyll degradation, low

production of C2H4, decrease in production rate of ascorbic acid, change in composition of unsaturated fat

and degradation of pectin were not fast as in nature. These things were reflected as the delay of mature of fruit and, therefore, storage capacity of the fruit is long (Amiarsi et al., 1996).

Fig 2. O2 Respiration rate of Balizalacca fruits during the storage time

CONCLUSION

 The weight of Balizalacca fruit was decreased during the storage time.

 At the beginning of storage time, the result showed that the respiration rate of O2 have been increased while in the middle have been decreased.

 We can use the polyethylene plastic packaging at perforation with 12 holes in modified atmosphere.

REFERENCES

Agung I.G.N, I.B.W. Gunam dan N.S. Antara. 1999. Pengaruh Panambahan Laktobacillus bulgaricus terhadap Mutu Sari Buah Zalacca Bali Selama Penyimpanan. Majalah Ilmiah Teknologi Pangan Gitayana, Vol. 5 (2): 34 – 39.

Amiarsi D., Sitorus, E., dan Sjaifullah. 1996. Pengaruh Teknik Penyimpanan Terhadap Mutu Buah Zalacca Lumut. J. Hort. Vol.6 No.4. 592-401.

Brecht P.E. 1980. Use of Controlled Atmosphere to Retard Deterioration of Produce. Food Technology 34(3) :45-50.

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Kader A.A. 1980. Prevention of Ripening in Fruits by Use of Controlled Atmosphere. Food Technology 34 (3) :51-54.

Labuza T.P., and W.M. Breene. 1989. Application of active Packaging for Improvement of shelf-life and

Nutritional Quality of Fresh and Extended Shellf-life Foods. Journal of Food Process Preservation 13 :

1-69.

Myers R.A. 1989. Packaging Consideration for Minimally Processed Fruits and Vegetables. Food Technology 43 : 129-131.

Suter I K. 1988. Telaah Sifat Buah Zalacca di Bali sebagai Dasar Pembinaan Mutu Hasil. Disertasi Doktor. Institut Pertanian Bogor, Bogor.

Song Yoonseok, Hyun Ku Kim, and Kit L. Yam. 1992. Respiration Rate of Blueberry in modified Atmosphere

Gambar

Fig 1. The changes of texture of Bali zalacca fruits during the storage time
Fig 2. O2 Respiration rate of Balizalacca fruits during the storage time

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