How to cite:
Sutiningsih D, Rahayu A, Puspitasari D (2017) The Level of Egg Sterility and Mosquitoes Age after the Release of Sterile Insect Technique (SIT) in Ngaliyan Semarang. J. Trop. Life. Science 7 (2): 133 – 137.
*Corresponding author: Dwi Sutiningsih
Department of Epidemiology and Tropical Disease Faculty of Public Health Faculty Diponegoro University Jalan Prof. Soedarto, Semarang, Indonesia 50275 E-mail: [email protected]
VOL. 7, NO. 2, pp. 133 – 137, April 2017 Submitted March 2016; Revised May 2016; Accepted February 2017
The Level of Egg Sterility and Mosquitoes Age after the Release of Sterile Insect Technique (SIT)
in Ngaliyan Semarang
Dwi Sutiningsih 1*, Ali Rahayu 2, Devi Puspitasari 1
1 Department of Epidemiology and Tropical Disease, Faculty of Public Health Diponegoro University, Semarang, Indonesia 2 National Nuclear Energy Agency (BATAN), Jakarta, Indonesia
ABSTRACT
Dengue control efforts have not provided adequate results. Therefore we need other measures like sterile insect Technique (SIT). This study aimed to observe the level of egg sterility and age of Aedes aegypti mosquitoes after using SIT in Ngaliyan Semarang. The method of this research is a descriptive study with a cross-sectional design. A sample of the mosquitoes eggs are all the mosquitoes eggs on the paper trap in every house of RW2 population in Ngaliyan village. These samples are the result of the fertilization of male and female mosquitoes in nature. Data analysis using Wilcoxon sign test. The result showed that the average of egg sterility percentage in mosquitoes after using SIT indoors was 73.65% and the average of egg sterility percentage outdoors was 69.08%. Wilcoxon test showed significant differences in average age of mosquitoes egg (p < 0.05), males pupae (p < 0.05), females pupae (p < 0.05), and adult female mosquitoes (p < 0.05), before and after SIT. It can conclude from this research that the SIT release in Ngaliyan village Semarang city could increase the level of sterility mosquito’s eggs and a decrease of the age of Ae. aegypti mosquitoes in this area.
Keywords: Sterile Insect Technique (SIT), egg sterility, mosquito’s age, Aedes aegypti, Ngaliyan INTRODUCTION
Dengue Fever is still a public health problem in In-donesia; it is likely to increase the number of cases and increasingly wide-spread. In Central Java, dengue fever cases in the period of May 2013, there were up to 10.000 cases, and 48 patients died, almost close to the total amount in 2012 that were up to 12.000 cases [1]. In Se-marang, the number of dengue fever cases in May 2013 reached 1.226 cases, approaching the total cases during 2012 with 8 patients died, in 2012 the number of dengue cases was at 1.250 with 22 patients died. From the inci-dence of dengue in 16 districts in Semarang, Ngaliyan was ranked first with incident rate (IR) 207 per 100,000 population in November 2013. Although up to March 2014 Ngaliyan was the third, the number of cases re-mained high and endemic repeatedly [2]. Appropriate vector control methods are needed. Some vector control methods that can be used include the use of chemical,
MATERIALS AND METHODS
This research uses a descriptive survey method and cross-sectional study design. Data were collected by ob-servation of Ae. aegypti in the field and laboratory. Sam-ples of the mosquito eggs are all mosquito eggs con-tained in filter paper in every house in RW 2 village Ngaliyan, resulted from natural breeding between sterile male mosquitoes and normal female mosquitoes. Sam-ple houses were 100 houses in RW 2 Ngaliyan Village when the new cases occurred at the same time with re-search. The data analyzed using univariate and bivariate analysis with Wilcoxon sign test.
RESULTS AND DISCUSSION Gametophyte development
Population growth of Ae. aegypti mosquitoes in RW 2 Ngaliyan Village is done by the release of 3000 sterile male mosquitoes every week. From Table 1 and 2, it showed that the number of Ae. aegypti eggs in the house are found more than the outside. This is because of the
Ae. aegypti mosquito prefers a dark, damp, and hidden places in the house or building, such as bedroom, closet, bathroom, and kitchen. These mosquitoes are rarely found outside the house, plants, or in another sheltered spot. Inside the room, Ae. aegypti resting under the house furniture, hanging objects such as clothes, cur-tains, as well as in walls [7]. Ae. aegypti mosquitoes usu-ally lay its eggs in the clean water reservoirs located in the house, such as water reservoirs, bathtubs, water jar and sink in the bathroom [8] Additional factor that is rainfall also affects populations of Ae. aegypti [9] The temperature has an effect on the activities of eating and the development rate of eggs into larvae, larvae into pu-pae and pupu-pae into imago [10]. The state of temperature causing stadium developmental disorders of mosquitoes so adult mosquitoes in the area may be more or less in order to produce eggs.
As seen on Tables 1 and 2, it is known that the av-erage egg sterility of Ae. aegypti found indoor (73.65%) was higher than outdoor (69.08%). This shows that 73.65% of the total egg (100%) in ovi-traps in the house did not hatch. It is one of the consequences of radiation on insects, namely infertility. Infertility in a general sense is the inability of an organism to produce offspring or inability to give the genome on the further descent. The mechanism of infertility can be caused by infecun-dity, dominant lethal mutation, the failure to mate, in-activation of sperm, and aspermia [11]. The evidence from the studies has shown that the radioactive rays were able to decrease the number of fertile eggs, for ra-
dioactive rays can change the structure of chromosomes with the presence of chromosomal aberrations and re-sulting abnormal form and arrangement of chromo-somes that it becomes sterile [12]. The study results from Purwanto and Sudaryadi [13]have proved that gamma-ray irradiation dose 100Gy (10 Krad) has an ef-fect in suppressing the development of pest eggs Bruchus by 16.33% (control) to 3.50% (irradiated ). Likewise, Handayani and Sudaryadi [14] research with treatment 100Gy of gamma ray irradiation (10 Krad) against the fruit flies Drosophila melanogaster male Meigen cause the mating results with normal females were able to suppress the first generation offspring (F1) by 89%.
The sterility of the eggs is one of the parameters of population decline [15]. Sterile eggs are eggs that do not contain an embryo [16]. The percentage of infertile eggs is calculated based on the eggs produced in each ovitrap. The increased egg sterility is caused by natural breeding of infertile males and normal females to produce infertile offspring [17]. The percentage of the sterility of mos-quito eggs outdoors reached the lowest at the release I. It can occur due to the natural female mosquitoes failed to mate with sterile male mosquitoes to produce sterile offspring of 66.67%. Effects of irradiation are able to cause changes in some genetic aspects of reproduction
Table 1. The percentage of sterility of Ae. aegypti mosquito eggs found indoor in the houses in the Ngaliyan village af-ter using SIT
No. Number of Sterility (%)
Egg indoor Mosquitoes indoor
1 675 291 56.89
Table 2. The percentage of sterility of Ae. aegypti mosquito eggs found outdoor in the Ngaliyan Village after using SIT
No. Number of Sterility (%)
Egg outdoor Mosquitoes outdoor
Table 3. The average age of Ae. aegypti at every stadium before and after the release of sterile male mosquitoes in the Ngaliyan village
Table 4. Wilcoxon test on the average age of Ae. aegypti after and before using SIT
mosquitoes after – before
-2.405 0.016 The age of adult female
mosquitoes after – before
-0.402 0.688
such as semi-sterile (decreased levels of fertility); de-creasing capacity of mating and reproductive capacity. The conclusion derived from the effects of this irradia-tion is that the treatment with irradiairradia-tion doses leads to acute doses. Carpenter stated that acute dose of reality has more effective influence than the chronic doses re-garding: vitality, mating capacity, reproductive capacity and mating competition [18]. The effect of radiation on fertility cannot be immediately visible because the post-gonial cells are relatively resistant to the lethal implica-tions of the influence of irradiation. If the sperm were irradiated at moderate to high doses, it will be found in the early rankings that remain fertile and then followed by a decline infertility or sterility. The length of time this infertility depends on the dose and the proportion of gonad cells, so temporary infertility does not result in significant changes in the hormonal balance, sexuality libido, or physical abilities. The post-gonial cells have a short lifespan; the gonad cells become necessary when
viewed in terms of fertility and genetically damage [19]. Therefore, it can be said that the probability or likeli-hood of permanent damage in a cell when the cell ex-posed by the radiation is determined by two main fac-tors, namely: the amount of radiation dose interacts with cells and the degree of the sensitivity of cells to radiation that is generally associated with the ability to hold their own repairments from individuals of the related cells [20].
Based on Table 3, the lifespan of the eggs starting from the hatching into larvae before using SIT is 4.7 days in average, while the lifespan of the mosquito eggs after using SIT is 4.51 days in average. The lifespan from the larval stage into female pupae before using SIT has the average of 4.35 days, and the average for male larvae is 4.40 days, while the lifespan from the larval stage after using SIT for female larvae the average is 3.75 days and the average for male larvae is 3.55 days. It takes approx-imately 2.47 days from pupal stage to become female adult mosquitoes before using SIT, and for male pupae, the average is 2.2 days, while the lifespan from the fe-male pupal stage after using SIT has the average of 2.7 days, and for male pupae, the average is 2.4 days. The average of lifespan before using SIT for adult female mosquitoes to death is 16.71 days, and for adult male mosquitoes the average is 14.14 days, while the average lifespan for adult female mosquitoes after using SIT is 14,5 days, and for adult male mosquitoes, the average is 12.95 days. Wilcoxon test showed no significant differ-ence in the average age of the mosquito eggs (p < 0.05), male pupae (p < 0.05), female pupae (p < 0.05) and the adult female mosquito (p < 0.05) before and after using SIT (Table 4). Long-life differences may occur due to differences in survival for each of mosquitoes and the possibility of abnormal development of natural mar-riages on male and female mosquitoes sterile [21].
the number of natural population will ensure that the next generation of gradual population growth has been narrowed to zero. The results of this study showed that male mosquitoes as the outcome from gamma ray irra-diation treatment are not completely sterile because they are still able to produce offspring population. So that it is known that gamma ray irradiation treatment Co-60 only cause Ae. aegypti males to become semi-sterile. The experimental results are consistent with the statement that the treatment radio-sterilized is intended to under-mine the chromosomal DNA of the mosquitoes’ sperm, and after mating, female mosquitoes will produce infer-tile eggs so it cannot successfully forward offspring or new generation [23].
CONCLUSION
The use of SIT in the Ngaliyan village can reduce the lifespan of mosquitoes and improve the eggs sterility of Ae. aegypti. The average percentage of the eggs ste-rility of Ae. aegypti found indoor (73.65%) was higher than outdoor (69.08%).
ACKNOWLEDGMENT
Acknowledgments addressed to all staffs of P2M DKK Semarang, Ngaliyan Health Center, Ngaliyan Vil-lage, PAIR BATAN, village cadres in Ngaliyan and stu-dents of Department of Epidemiology and Tropical Dis-ease, Public Health Faculty, Diponegoro University, who have helped in the implementation of the surveys and data collection in the field.
REFERENCES
1. Balitbangkes (2004) Kajian kesehatan demam berdarah den-gue. Jakarta, Depkes RI.
2. Zuhdiar L (2013) Dinkes Semarang waspadai lonjakan kasus DBD. http://www.antarajateng.com/. Accessed: February 2014.
3. Dinas Kesehatan Kota Semarang (2014) Laporan jumlah ka-sus demam berdarah di Kota Semarang 2014. http://www.depkes.go.id/. Accessed: February 2014. 4. Simanjuntak H (2005) Efektivitas akar tanaman Tuba
(Der-ris elliptica) untuk pengendalian nyamuk Anopheles sp. Bachelor Thesis. Universitas Sumatera Utara, Fakultas Kesehatan Masyarakat.
5. Sudaryadi I (2006) Dampak iradiasi sinar Gamma Co-60 ter-hadap beberapa aspek genetika reproduksi nyamuk Aedes albopictus Skutse. Master Thesis. Universitas Gadjah Mada. Fakultas Kesehatan Masyarakat.
6. Soegijanto S (2014) Demam berdarah dengue. Surabaya, Air-langga University Press.
7. Romero-Vivas CME, Falconar AKI (2005) Investigation of relationships between Aedes aegypti egg, larvae, pupae, and adult density indices where their main breeding sites were located indoors. Journal of the American Mosquito Control Association 21: 15 – 21.
8. Vloedt AMV, Klasen W (2014) The development and appli-cation of the sterile insect technique (SIT) for new world screwworm eradication. http://www.fao.org/. Accessed: June 2014.
1. Balitbangkes (2004) Kajian Kesehatan Demam Berdarah Dengue. Jakarta: Depkes RI.
2. Zuhdiar L. Dinkes Semarang waspadai lonjakan kasus DBD 2013
[http://www.antarajateng.com/detail/index.php?id=80089] accessed date February 1, 2014.
3. Dinas Kesehatan Kota Semarang (2014) Laporan Jumlah Kasus Demam Berdarah di Kota Semarang. Semarang: DKK Semarang
4. Simanjuntak H (2005) Efektivitas akar tanaman Tuba (Derris elliptica) untuk pengendalian nyamuk Anopheles sp. Skripsi. Universitas Sumatera Utara, Fakultas Kesehatan Masyarakat.
5. Sudaryadi I (2006) Dampak iradiasi sinar Gamma Co-60 terhadap beberapa aspek genetika reproduksi nyamuk Aedes albopictus Skutse. Tesis. Universitas Gadjah Mada, Program Studi Ilmu Kedokteran Dasar dan Biomedis.
6. Soegijanto S (2014) Demam Berdarah Dengue. Surabaya: Airlangga University Press.
7. Romero-Vivas CME, Falconar AKI (2005) Investigation of relationships between Aedes aegypti egg, larvae, pupae, and adult density indices where their main breeding sites were located indoors. J Am Mosq Control Assoc. 21:15–21. 8. Vloedt AMV, Klasen W (2014) The development and application of the sterile insect technique (SIT) for new
world screwworm eradication
[http://www.fao.org/ag/aga/agap/FRG/FEEDba ck/ War/u4220b/u4220b0j.html] accessed date June 1, 2014. 9. Brady OJ, Golding N, Pigott DM et al. (2014) Global
tem-perature constraints on Aedes aegypti and Ae. albopictus persistence and competence for dengue virus transmission. Parasit Vectors 7: 338-348.
10. Delatte AH, Gimonneau G, Triboire A et al. (2009) Influ-ence of temperature on immature development, survival, longevity, fecundity, and gonotrophic cycles of Aedes al-bopictus, vector of chikungunya and dengue in the Indian Ocean. Journal of Medical Entomology 46 (1): 33–41 11. Bloem KA, Bloem S, Carpenter JE (2005) Impact of moth
and practice in area-wide integrated pest management. Dor-drecht, Springer. pp 677-700.
12. Marec F, Neven L, Robinson AS et al. (2005) Development of genetic sexing strains in Lepidoptera: from traditional to transgenic approaches. Journal of Economic Entomology 98 (2): 248-259.
13. Purwanto H, Sudaryadi I (2002) Pengendalian hama dan penyakit benih kedelai dalam kemasan dengan radiasi sinar gamma Co-60. Jakarta, Balitbang Pertanian.
14. Handayani NSN, Sudaryadi I (1997) Pengaruh radiasi sinar gamma Co-60 terhadap penekanan populasi Drosophila melanogaster tipe liar (Laporan Penelitian BPPF). Yogyakarta, Universitas Gadjah Mada.
15. Alphey L, Benedict M, Bellini R et al. (2014) Sterile-insect methods for control of mosquito - borne diseases: An analysis. Vector Borne and Zoonotic Diseases 10 (3): 295-311. doi: 10.1089/vbz.2009.0014.
16. Vreysen MJB, Hendrichs J, Enkerlin WR (2006) The steril insect technique as a component of suistanable area-wide integrated pest management of selected horticultural insect pest. Journal of Fruit and Ornamental Plant Research 14 (suppl 3): 107-131.
17. Barrera R, Amador M, Clark GG (2006) Ecological factors influencing Aedes aegypti (Diptera : Culicidae) productivity
in artificial containers in Salinas, PuertoRico. Journal of Medical Entomology 43 (3): 234–239.
18. Carpenter JE, Bloem KA, Bloem S (2001) Aplication of F1 sterility for research and management of Cactoblastic cacto-rum (Lepidoptera: Pyralidae). Florida Entomologist 84 (4): 531-536.
19. Songsri P, Suriharn B, Sanitchon J et al. (2011) Effect of gamma radiation on germination and growth characteristic of physic nut (Jatropa curcas L). Journal of Biological Sci-ences 11 (3): 268274. doi: 10.3923/jbs.2011.268.274. 20. Desai AS, Rao S (2014) Effect of gamma radiation on
germi-nation and physiological aspects of Pigeon Pea (Cajanus ca-jan L. Millsp) seedlings. IMPACT: International Journal of Research in Applied, Natural and Social Sciences 2 (6): 4752.
21. Dyck VA, Hendrichs J, Robinson AS (2005) Sterile insect technique: Principles and practice in area-wide integrated pest management. Dordrecht, Springer.
22. Knipling E (1959) Sterile-male method of population control. Science 130 (3380): 902–904.
