A B C D E

A

B C

CFA 1W

40 mV

40 pA Saline 4hCFA 4h

Saline 1WCFA 1W 0

10 20 30 40 50

Time in center (s)

0 20 40 60 80

Time in open arms (s)

Saline 4hCFA 4h

Saline 1WCFA 1W EPMT OFT

Saline 1W CFA 4h Saline 4h

40 pA 200pA

400 ms 400 ms

40 mV

n.s.

*** ** n.s.

0 1000 2000 3000 4000

Total distances (cm)

Saline 4hCFA 4h

Saline 1WCFA 1W

n.s. n.s.

0 40 80 160 200

0 5 10 15 20 25

Injected current (pA)

Firing rate (Hz)

120 CFA 4h Saline 4h

0 40 80 120 160 200 0

5 10 15 20 25

Injected current (pA)

Firing rate (Hz) ***

CFA 1W Saline 1W

D

0 50 100 150 200

Saline 4h CFA 4h n.s.

Rheobase (pA)

OFT

E

0 50 100 150

200 *

Saline 1W CFA 1W

Rheobase (pA)

200pA

0 1000 2000 3000 4000

Total distances (cm)

Saline 4hCFA 4hSaline 1W CFA 1W

0 20 40 60

80 EPMT

Time in open arms (s)

0 20 40

60 OFT

Time in center (s)

0 10 20 30

Total entries

Saline 4hCFA 4hSaline 1W CFA 1W

20 lux 70 lux 120 lux

Saline 4hCFA 4hSaline 1W CFA 1W Saline 4hCFA 4hSaline 1W

CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4hCFA 4hSaline 1W CFA 1W

Saline 4h Saline 1WCFA 4h CFA 1W

20 lux 70 lux 120 lux

B

50

30

10

5 15 25

***

^n.s.

*

^n.s.

***

^n.s.

**

^n.s.

**

n.s.

**

n.s.

**

^n.s.

***

^n.s.

***

^n.s.

n.s. n.s.

n.s. n.s.

n.s. n.s.

OFT

EPMT

PF S2

Contralateral S1

VPL

SNr

S2 : mCherry Contralateral S1 : mCherry VPL : mCherry

PO : mCherry PF : mCherry SNr : mCherry

Po

B

S1HL5 : mCherry

S1HL5

AAV-DIO-mCherry

CaMKII-Cremouse S1HL5

S1HL5 : mCherry

C

A

cDLS

GFP : DsRed cDLS : GFP : DsRed

AAV-DIO-TVA-GFPDay1 AAV-DIO-RVG

Day 21

RV-EnvA-ΔG-DsRed cDLS

S1HL5

GAD2-Cremouse

A

Motor cortex Visual cortex

Auditory cortex

PF SNr PO

BLA

DRN

Motor cortex PF PO BLA

Visual cortex Auditory cortex SNr DRN

C

GABA

DsRed Merged

0 20 40 60 80 100

Percentage (%)

RV+GABA / RV

C D

A

B

E

F

SOM

mCherry Merged

mCherry PV Merged

AAV-DIO-mCherry AAV2/1-hSyn-Cre

cDLS S1HL5

cDLS : mCherry

D

40 mV

400 ms

400 pA 560 pA

Saline 1W

CFA 1W

C

160 320 480 640

0 10 20 30 40

Injected current (pA)

Firing rate (Hz)

Saline 1W CFA 1W

0 100 200 300 400 500

Rheobase (pA)

Saline 1W CFA 1W n.s.

0 10 20 30 40

Firing rate (Hz)

160 320 480 640

Injected current (pA) Saline 4h

CFA 4h

400 pA 560 pA

40 mV

400 ms Saline 4h

CFA 4h

0 200 400 600

Rheobase (pA)

Saline 4h CFA 4h n.s.

hM4Di-mCherry mCherry

0 -5 -10 -15 V ∆(mV)

hM3Dq-mCherry 5

10 10 μM CNO

1 min 6 mV

***

**