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Appendix 1: Summary of the DTI Harmonization Guide for Bivalirudin and Argatroban 1. Pre-VAD implantation work-up ( <48 hours pre VAD):

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Appendix 1: Summary of the DTI Harmonization Guide for Bivalirudin and Argatroban 1. Pre-VAD implantation work-up ( <48 hours pre VAD):

 Baseline labs: CBC with diff, aPTT*, PT/INR, fibrinogen, basic metabolic panel (BMP)

 Optional labs: TEG with PM, CRP, LDH, cystatin C, HIT screen, ROTEM

2. Intra-op management

 Standard heparin anticoagulation for cardiopulmonary bypass with full protamine reversal in OR

 Standard blood product replacement to normalize coagulation parameters and establish hemostasis in OR

3.

Early post-op management

 Labs (aPTT, PT/INR, fibrinogen, BMP, CBC) within 2 hours of arrival to ICU

 Optional: dilute thrombin time (dTT), anti-Xa (heparin activity level), TEG ± PM, ROTEM

 It appears reasonable to start DTI once:

 Surgical and coagulopathic bleeding resolved ( < 2 ml/kg of chest tube output for 4 hours and no other sources of active bleeding)

 aPTT within 15 sec of baseline* ( or institutional normative range)

 INR <1.3

 Fibrinogen >100

 Platelet count >100,000

 Correct with blood product replacement as needed, being mindful of risk of dilutional coagulopathy with multiple transfusions, and correct any surgical bleeding as needed

*Baseline aPTT may be elevated if previously on anticoagulation agent, therefore use age/intuitional normative range

**Given the risk of early bleeding, most of patients will begin in the “High risk” category for the first several days on support, then transition to “Standard risk”.

 Check aPTT 2 hours after first initiation. Cautious about titrating with first level.

o If aPTT has jumped dramatically to >2-3 x baseline PTT, then decrease Bival by 50% and recheck in 2-3 hours

o If PTT has increased to 1-1.5 x baseline, make no adjustment and repeat PTT in 2-3 hours as level may continue to rise

Initial Bivalirudin Dosing Goal: aPTT

Standard risk (of bleeding): aPTT 2-3 x baseline(60-90)

**High risk (of bleeding): aPTT 1.5-2 x baseline (45-60)

Renal function Initial dosing

Normal (>60ml/min/1.73 m2) 0.3 mg/kg/hr IV infusion

Mild-moderate (30-60ml/min/1.73 m2) 0.2 mg/kg/hr IV infusion Severe (<30ml/min/1.73 m2) 0.1 mg/kg/hr IV infusion

Maintenance Bivalirudin/Argatroban titration Goal: aPTT

Standard risk (of bleeding): aPTT 2-3 x baseline (60-90) High risk (of bleeding): aPTT 1.5-2 x baseline (45-60)

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aCenters that used argatroban followed the same recommendations for baseline and follow up laboratory work as well as achieving prompt hemostasis followed by initiation of argatroban at 0.5mg/kg/min intravenous infusion and titrating aPTT to 1.5-2x baseline for first 12-24 hours and then uptitrating to 2-3x baseline if hemostasis was maintained. If patients had hepatic dysfunction, then starting dose would be decreased accordingly.

If aPTT 5 to 15 sec out of range

Increase or decrease by 0.03 mg/kg/hr OR

Increase or decrease by 10%

If aPTT 1.5X-3X baseline No change

If aPTT >15-30 sec out of range

Increase or decrease by 0.05 mg/kg/hr or Increase or decrease by 20%

If aPTT >3x baseline (~ 100 sec)**

BIVALIRUDIN:

If normal renal function- hold 15 min and by 30%

If mild to moderate renal dysfunction- hold for 45 min and reduce by 40%

If severe renal dysfunction- hold 2 hours and recheck PTT before starting ARGATROBAN:

With normal hepatic function: hold 30 min and reduce by 30%

With mild to moderate hepatic dysfunction: hold for 60 min and reduce by 40%

With severe hepatic dysfunction: hold 2 hours and recheck PTT before restarting

Initial Argatroban Dosinga Goal: aPTT

Standard risk (of bleeding): aPTT 2-3 x baseline(60-90)

**High risk (of bleeding): aPTT 1.5-2 x baseline (45-60)

Hepatic function Initial dosing

Normal 0.5 mg/kg/hr IV infusion

Mild-moderate 0.3 mg/kg/hr IV infusion

Severe 0.2 mg/kg/hr IV infusion

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