Supplemental Material
Supplemental Tables. Changes in pupil diameters, heart rate, mean arterial blood pressure and numeric rating scale.
Change in pupil diameter
ANOVA-Type Statistic (ATS) Between-group effect: P = 0.011 Time effect: P < 0.001
Group * Time interaction effects: P = 0.507
Multiple comparisons of pupil diameters
P-value Adjusted P-value (P-value multiplied by 5) Baseline vs. surgical incision P < 0.001 P < 0.001
Baseline vs. intraperitoneal gas insufflation
P < 0.001 P < 0.001 Baseline vs. trocar insertion P < 0.001 P < 0.001 Baseline vs 5 min after trocar
insertion P < 0.001 P < 0.001
Baseline vs 10 min after trocar insertion
0.233 0.999
Change in heart rate
ANOVA-Type Statistic (ATS) Between-group effect: P = 0.927 Time effect: P < 0.001
Group * Time interaction effect: P = 0.398
Multiple comparisons of heart rate
P-value Adjusted P-value (P-value multiplied by 5) Baseline vs. surgical incision P < 0.001 P < 0.001
Baseline vs. intraperitoneal
gas insufflation P < 0.001 P < 0.001 Baseline vs. trocar insertion P < 0.001 P < 0.001 Baseline vs 5 min after trocar
insertion
P < 0.001 P < 0.001 Baseline vs 10 min after
trocar insertion
P < 0.001 P < 0.001
Change in mean arterial blood pressure
ANOVA-Type Statistic (ATS) Between-group effect: P = 0.797 Time effect: P < 0.001
Group * Time interaction effect: P = 0.774
Multiple comparisons of mean arterial blood pressure
P-value Adjusted P-value (P-value multiplied by 5) Baseline vs. surgical incision P < 0.001 P < 0.001
Baseline vs. intraperitoneal
gas insufflation P < 0.001 P < 0.001 Baseline vs. trocar insertion P < 0.001 P < 0.001 Baseline vs 5 min after trocar
insertion
P < 0.001 P < 0.001 Baseline vs 10 min after
trocar insertion
P < 0.001 P < 0.001
Change in NRS
ANOVA-Type Statistic (ATS) Between-group effect: P = 0.003 Time effect: P < 0.001
Group * Time interaction effect: P = 0.753
Multiple comparisons of NRS
P-value Adjusted P-value (P-value multiplied by 5) Admission to PACU vs. 10
min
0.019 0.095
Admission to PACU vs. 20 min
< 0.001 < 0.001 Admission to PACU vs. 30
min
< 0.001 < 0.001 Admission to PACU vs. D/C < 0.001 < 0.001 Admission to PACU vs. POD1 < 0.001 < 0.001
Supplemental Figures
The data of the box plots show median (line within the box), 1st and 3rd quartiles (lower and upper boundaries of the box), and 10th and 90th percentiles (whiskers below and above the box). *P <0.001 compared to baseline. Nonparametric rank-based method28 was used to analyze the data. T1: 10 min after induction of anesthesia, T2: Surgical incision, T3:
Intraperitoneal gas insufflation, T4: Trocar insertion, T5: 5 minutes after trocar insertion, T6:
10 min after trocar insertion, SPI: surgical pleth index.
Supplemental Figure 1. Longitudinal change in pupil diameter during surgery. P values for between-group, time, and group*time interaction effects are 0.011, <0.001, and 0.507, respectively.
Supplemental Figure 2. Longitudinal change in heart rate during surgery. P values for between-group, time, and group*time interaction effects are 0.927, <0.001, and 0.398, respectively.
Supplemental Figure 3. Longitudinal change in mean arterial blood pressure during surgery.
P values for between-group, time, and group*time interaction effects are 0.797, <0.001, and 0.774, respectively.
