- Calculation
Rf of turmeric
Rf average of turmeric=0,872+0,855
2 =0,8635
Rf of suji leaf
Rf average of suji=0,909+0,927
2 =0,918 - Answer Question
1. What will happen if the eluent is used as the developer solvent in TLC too polar or less polar? Why?
Answer:
If the eluent used as the developer solvent in TLC is too polar then all the spots that are placed on the plate rise to the upper limit of the plate (solvent front) without experiencing separation.
If the eluent used as developer solvent in TLC is less polar then the spot that is applied doesn't move at all.
2. What is the function of filter paper in the Rf determination experiment?
Answer:
The function of filter paper in the Rf determination experiment is used so that the atmosphere in the glass is (chamber) is saturated with solvent vapor (saturating the chamber).
3. Why should the surface of the TLC plate not be damaged?
Answer:
The surface of the TLC plate must not be damaged because it can affect the separation results blemishes or development of blemishes 4. Why does the TLC plate that has to be used have to be dried in the
oven first?
Answer:
The TLC plate used must first be dried in an oven at 110o
Before used to remove bound water molecules and activate absorbent.
Because water is strongly bound to the absorbent, it can inhibit the occurrence of equilibrium with the analyte molecules.
5. Why should the upper and lower limits of the plate be marked with a pencil?
Answer:
The upper and lower limits of the plate must be marked with a pencil because if you use a ballpoint pen, the ballpoint stain will also be eluted or expand.