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• BLOOD PROFILE AND NUTRIENTS DIGESTIBILITY OF SUPER NATIVE CHICKENS FED FUNGTIONAL DIETS WITH DIFFERENT ENERGY AND

PROTEIN LEVEL

Hafsah1, Sri Sarjuni1, Selvi Mozin1, Fatmawati1, Amiruddin Malewa1, I. N. Sucipto2, Syamsul Saifuddin3 1Animal Science Department Faculty of Animal Husbandry and Fishery, Tadulako University

2 Under Graduate Student of Animal Science Department, Tadulako University 3 Research Staf of LITBANG Province of Central Sulawesi, Indonesia

Email: hafsah_62@yahoo.co.id

THE 3rd INTERNATIONAL CONFERENCE OF ANIMAL SCIENCE & TECHNOLOGY

TADULAKO UNIVERSITY, PALU

Makassar, November 03- 2020

Theme:” Animal Production and Sustainable Agriculture for Food

Security in The Tropical Environment”

BLOOD PROFILE AND NUTRIENTS DIGESTIBILITY OF SUPER NATIVE CHICKENS FED FUNGTIONAL DIETS WITH DIFFERENT ENERGY AND PROTEIN LEVEL

Hafsah1, Sri Sarjuni1, Selvi Mozin1, Fatmawati1, Amiruddin Malewa1, I. N. Sucipto2, Syamsul Saifuddin3 1Animal Science Department Faculty of Animal Husbandry and Fishery, Tadulako University

2 Under Graduate Student of Animal Science Department, Tadulako University 3 Research Staf of LITBANG Province of Central Sulawesi, Indonesia

Email: hafsah_62@yahoo.co.id

BLOOD PROFILE AND NUTRIENTS DIGESTIBILITY OF SUPER NATIVE CHICKENS FED FUNGTIONAL DIETS WITH DIFFERENT ENERGY AND PROTEIN LEVEL

01

INTRODUCTION This contains information

about the background, problem formulation,

research objectives

02

OBJECTIVE The present study aimed to Determine

the blood profle, pH intestine, & nutrient Digestibility of super native

Chicken fed functional Diets with different energy

& protein level

03

RESEARCH METHODS This contains information

About the research method, Time & place,

the sample Of the research Instrument Data Analysis technique

04

RESULT & DISCUSSION This contains information

About the result &

Discussion Of the research data

obtained

05

CONCLUSIONS AND SUGGESTIONS

This contain information about Research

Conclusions And Suggestions

01

Functional Feed

Local Feed +

(Moringa O + Tumeric) Genetics

Environment

Non Functional Feed

Animal Productiity

Fungtional Diets

Processing

70% feeds

02

Objective

The present study aimed to determine the blood profile, pH intestine, and nutrient digestibility of super native chicken fed fungtional diets with different energy and

protein level

03

1. The experiment was conducted at the Teaching Farm of Animal Science Department, Faculty of Animal Husbandry and Fishery Tadulako University, Palu Central Sulawesi

2. Experimental chicken used 192 DOC of Super Native Chicken that rearing up to 14 weeks old, and kept in slat cage.

3. Experimental Diets

The fungtional feed used yellow corn grain, rice bran, fish meal, soybeans, moringa leaves meals, tumeric flour, cocnut oil, and top mix. The proximate analysis of feed inggredients were analyzed using AOAC methods (2006).

Composition of feed inggredients as shown in Table 1.

Treatment diets consist of: Energy (EM1 & EM2 ) and Protein (P1 ; P2 ; P3 ; P4 )

II. Research Methods

03

Table 1.Diets Composition and Nutrient Contents of the Treatment

Diets Compositions (%)

Energy Treatment (EM₁) (2.800 kcal)

Energy Treatment (EM₂) (3.000 kcal)

P₁ (18%)

P₂ (19%)

P₃ (20%)

P₄ (21%)

P₁ (18%)

P₂ (19%)

P₃ (20%)

P₄ (21%)

Yellow corn grain Rice bran

Soybean grain Fishmeal

MO leaves flours Curcuma flours Coconut oil Top mix

60 13 10 9 5 1 1 1

60 11.5

10 10.5

5 1 1 1

56.5 12 12 12 5 1 1 1

56 11 10 15 5 1 1 1

61 8 14

7 5 1 1 1

60.5 8 11 10.5

5 1 1 1

59 7.5

11 12.5

5 1 1 1

56.5 7 14 12.5

5 1 1 1

Total 100 100 100 100 100 100 100 100

03

Table 1.Diets Composition and Nutrient Contents of the Treatment

Noted: ¹⁾ Calculated from proximate analysis of each feedstuffs (Hafsah et al. 2016) ²⁾ Calculated from NRC (1994)

Nutriens Contents

Energy Treatment (EM₁) (2.800 kcal)

Energy Treatment (EM₂) (3.000 kcal)

P₁ (18%)

P₂ (19%)

P₃ (20%)

P₄ (21%)

P₁ (18%)

P₂ (19%)

P₃ (20%)

P₄ (21%)

EM, kcal/kg^{1) 2.815 2.835 2.830 2.840 3.006 3.001 3.006 3.017} Crude Protein, %^{1) 18.25 19.00 20.15 21.33 18.19 19.07 20.10 21.02} Crude Fat, %^{1) 5.13 5.14 5.43 5.20 5.53 5.17 5.20 5.60} Fiber, %^{1) 6.33 6.07 6.17 5.84 5.72 5.51 5.37 5.39}

Ash, %^{1) 5.24 5.29 5.58 5.92 4.45 4.87 5.12 5.17}

Ca, %^{2) 0.62 0.70 0.76 0.93 0.53 0.70 0.80 0.81}

P, %^{2) 0.66 0.68 0.71 0.78 0.55 0.64 0.69 0.68}

Lysine, %^{2) 0.75 0.81 0.86 1.00 0.65 0.80 0.88 0.89} Methionine, %^{2) 0.32 0.34 0.35 0.40 0.28 0.33 0.36 0.36}

03

4. Experimental Design and Treatments

Design of experimental using Factorial Randomized Design with two factors . The firts factor is energy level ie. (EM1 = 2.800 kcal; EM2 = 3.000 kcal), and the second factor is protein level i.e. (P1 = 18%; P2

=19%; P3 =20%; P4 = 21%)

The treatment composition as shown in Table 1.

Feed treatment was applied to chicken aged from 2-12 weeks old, the sample of blood profile taken at 12 weeks old, and nutrient digestibility 12-13 weeks old, and pH intestine in 13 weeks old.

5. Variable Observed

Blood profile, small intestine pH, and nutrient digestibility

6. Data Analysis

Using ANOVA in SPSS 16, and DMRT

04

III. Results

Table 2. Average Blood Profile of each treatment

Blood Profile

Energy (Kcal)

Protein (%)

P₁ P₂ P₃ P₄ P

Hb (g/dl

EM₁ 11.10^a 8.70^b 10.20^a 10.55^a

*

EM₂ 10.30 10.30 10.45 11.20 ns

Leucicyte (10³ /m³ )

EM₁ 130.06^a 128.80^a 113.90^b 114.95^b * EM₂ 124.10^a 122.30^a 107.40^b 105.15^b * Eritrocyte

(10⁶/mm³ )

EM₁ 2530^a 1940^b 2325^a 2330^a *

EM₂ 2370 2210 2250 2400 ns

Hematocrite (%)

EM₁ 35.25 26.45 30.65 31.05 *

EM₂ 30.75 30.55 30.35 32.60 ns

Trombosite

EM₁ 70000^a 70500^a 56000^b 53500^b * EM₂ 49000^a 43000^b 40000^b 36000^b * Cholesterol

Total

EM₁ 121.00^a 111.50^b 125.5^a 109.50^b **

EM₂ 124.50 128.00 124.00 119.50 ns

3.1. Blood Profile

04

Energy (kcal)

Protein (%)

Mean P

P

₁

P

₂

P

₃

P

₄

EM

₁

6.25 5.96 6.11 6.07 6.10

ns

EM

₂

6.11 6.24 6.09 6.07 6.13

ns

Mean 6.18 6.16 6.10 6.07

3.2. pH Intestine

Table 3. Average pH intestine of each treatment

04

3.3. Nutrient Digestibility

Table 4. Average Nutrient Digestibility of each treatment

Nutrient

Digestibility Energy (Kcal)

Protein (%)

P

₁

P

₂

P

₃

P

₄

P

Dry Matter

(%) EM

₁

83.61 80.33 79.98 80.08

^ns

EM

₂

83.52 80.31 78.72 79.88

^ns

Crude Protein

(%) EM

₁

73.34 74.44 73.54 74.20

^ns

EM

₂

72.62 71.36 74.37 74.77

^ns

Crude Fat

(%) EM

₁

93.64 88.95 88.21 81.39

^ns

EM

₂

91.52 91.15 87.32 87.09

^ns

05

Conclusion & Recomendation

1. The results showed that the using of different energy and protein levels in fungtional diets could be affect the blood profile (hematocrite, bilirubin, and total cholesterol), however it does not affect the pH intestine and nutrient digestibility of super native chickens

2. The treatment gave best results from this study : diets formula in the treatment of EM

₁

P

₁

(EM = 2.800 kcal and Protein 18%) 3. Recomendation of this study in the growth rate of super native

chicken, it is recomended to use the diets formula with the

EM= 2.800 kcal and Protein 18%.

05

Acknowledgement

1. The authors would like to thanks to Ministry Research, Technology and Higher Education, that provided research funding in the skim project of excellent research universities (PTUPT) in 2017

2. Also many thanks to ICAST 3 Commite for give me a

chance to joint this seminar

Thanks for yours

attention