ELECTRONIC SUPPLEMENT
eFigure 1. Plasma Ang-2 was negatively correlated with platelet count while plasma Ang-1 was positively
correlated.
eFigure 2. Correlation of Ang-2 levels with days alive and free of pressors or mechanical ventilation, or
with composite outcomes involving any combination of pressors, mechanical ventilation, and renal
failure. Renal failure was defined as a moderate to severe Brussels score, determined by a plasma
creatinine greater than 2.0mg/dL.
eFigure 3: Plasma Ang-2 was associated with increased plasma lactate on days 1-5.
eFigure 4. Plasma lactate was associated with fluid overload on days 0-3.
eFigure 5. Increased level of plasma Ang-2 was associated with a requirement for the use of acute
corticosteroids.
eFigure 6. Plasma Ang-2 levels positively correlated with CVP, measured at the time of lowest MAP, for
days 0-3.
eFigure 7. Plasma Ang-2 levels negatively correlated with days alive in patients who died within 28 days.
eFigure 8. Plasma Ang-2 levels were not associated with the use of norepinephrine or vasopressin.
eTable 1. Summary of spearman correlation of log transformed plasma Ang-2 and 1 levels with log- transformed plasma cytokine levels.
Ang-2 Ang-1
Baseline 24 Hours Baseline 24 Hours
Cytokine Spearman r P-value Spearman r P-value Spearman r P-value Spearman r P-value CCL11 0.2156 0.0002 0.137 0.0169 -0.006732 0.9068 0.009159 0.8734 CCL2 0.397 < 0.0001 0.3404 < 0.0001 -0.1465 0.0104 -0.1739 0.0023 CCL22 0.09104 0.1132 -0.03158 0.5833 0.1102 0.0545 0.09961 0.0824 CCL3 0.316 < 0.0001 0.2241 < 0.0001 -0.15 0.0087 -0.1597 0.0052 CCL4 0.3267 < 0.0001 0.2175 0.0001 -0.08072 0.1597 -0.09266 0.1063 CCL7 0.343 < 0.0001 0.2063 0.0003 0.03558 0.5359 -0.006367 0.9118 CD40LG -0.02374 0.6802 -0.1139 0.0472 0.5167 < 0.0001 0.3856 < 0.0001 CSF2 0.3334 < 0.0001 0.2719 < 0.0001 -0.04925 0.3914 -0.06978 0.2243 CSF3 0.3834 < 0.0001 0.2599 < 0.0001 -0.1795 0.0017 -0.1757 0.0021 CX3CL1 0.3029 < 0.0001 0.1708 0.0028 0.01153 0.8411 0.01648 0.7743 CXCL1 0.06935 0.228 -0.09493 0.0985 0.3845 < 0.0001 0.2747 < 0.0001 CXCL10 0.2685 < 0.0001 0.2144 0.0002 -0.1744 0.0022 -0.1784 0.0018
EGF 0.2059 0.0003 0.09729 0.0904 0.1728 0.0025 0.1239 0.0305
FGF2 0.2745 < 0.0001 0.1101 0.0553 0.07012 0.222 0.05628 0.3272 FLT3LG 0.1751 0.0022 0.0751 0.1916 -0.03608 0.5302 -0.05611 0.3287 IFNA2 0.31 < 0.0001 0.1558 0.0065 0.04044 0.4816 0.03322 0.5633 IFNG 0.2415 < 0.0001 0.1037 0.071 -0.02341 0.6838 -0.009151 0.8735 IL10 0.376 < 0.0001 0.2685 < 0.0001 -0.1653 0.0038 -0.1843 0.0012 IL12B 0.2816 < 0.0001 0.1404 0.0143 0.02088 0.7165 0.02349 0.6829 IL12P70 0.3237 < 0.0001 0.1562 0.0064 -0.01274 0.8246 -0.004562 0.9368 IL13 0.2589 < 0.0001 0.06187 0.2823 0.007481 0.8965 -0.02717 0.6365 IL15 0.3361 < 0.0001 0.2818 < 0.0001 -0.004094 0.9432 -0.04416 0.4422 IL17A 0.3055 < 0.0001 0.1745 0.0023 -0.06711 0.2426 -0.04433 0.4404 IL1A 0.2829 < 0.0001 0.2237 < 0.0001 -0.05733 0.3183 -0.04494 0.4342 IL1B 0.2164 0.0001 0.05489 0.3402 0.009261 0.872 0.004781 0.9337 IL1RN 0.3449 < 0.0001 0.2318 < 0.0001 -0.05124 0.3725 -0.03254 0.5714 IL2 0.232 < 0.0001 0.09715 0.0909 -0.02059 0.7202 -0.03864 0.5014 IL2RA 0.4031 < 0.0001 0.3153 < 0.0001 -0.05562 0.333 -0.02155 0.7078
IL3 0.1197 0.037 0.06556 0.2545 -0.07375 0.199 -0.04767 0.4068
IL4 0.2496 < 0.0001 0.1217 0.0339 0.01959 0.7333 -0.02489 0.6651
IL5 0.06874 0.2321 -0.02256 0.6953 0.03412 0.5528 0.04531 0.4304
IL6 0.3918 < 0.0001 0.2945 < 0.0001 -0.09601 0.0942 -0.1146 0.0455
IL7 0.3053 < 0.0001 0.09312 0.1051 0.02784 0.6282 0.04835 0.4001
IL8 0.4824 < 0.0001 0.3825 < 0.0001 -0.1509 0.0083 -0.1597 0.0052
IL9 0.2604 < 0.0001 0.1422 0.013 -0.02168 0.7061 -0.04098 0.4758
LTA 0.2474 < 0.0001 0.08887 0.1221 0.003349 0.9536 -0.03144 0.5844 TGFA 0.2968 < 0.0001 0.172 0.0026 0.1001 0.081 0.01357 0.8134
TNF 0.5065 < 0.0001 0.413 < 0.0001 -0.1547 0.0068 -0.1531 0.0074 VEGFA 0.299 < 0.0001 0.1713 0.0027 0.05622 0.3278 0.03381 0.5563