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Table E1. Shapiro-Wilk W test for normal data. N = number of observations, W = Shapiro- Wilk test statistic. P values denote probability of non-normal distribution (P<0.0001 for completely non-normal data with N=7).
Experimental condition
Variable N W P value
Baseline
Mean systemic artery pressure 7 0.872 0.194 Mean pulmonary artery presure 7 0.962 0.837 Cardiac output 7 0.930 0.550 Mean airway pressure 7 0.937 0.611 Tidal volume 7 0.822 0.067 Arterial oxygen tension 7 0.854 0.134 Arterial carbon dioxide tension 7 0.975 0.931 Alveolar-arterial oxygen tension difference 7 0.874 0.201 Mean log (V/Q) 7 0.894 0.294 Mean SDlog (V/Q) 7 0.896 0.309
Qcvtotal 7 0.957 0.792
Qcvgravitational 7 0.962 0.839
Qcvisogravitational 7 0.925 0.511
Vcvtotal 7 0.809 0.050
Vcvgravitational 7 0.944 0.678
Vcvisogravitational 7 0.981 0.964
Q β1 7 0.917 0.449
V β1 7 0.935 0.595
V/Q β1 7 0.938 0.626
Baseline 2
Mean systemic artery pressure 5 0.964 0.837 Mean pulmonary artery presure 5 0.975 0.908 Cardiac output 5 0.826 0.129 Tidal volume 5 0.892 0.368 Arterial oxygen tension 5 0.912 0.482 Arterial carbon dioxide tension 5 0.869 0.261 Alveolar-arterial oxygen tension difference 5 0.985 0.958
Qcvtotal 5 0.916 0.502
cNOS inhibition
Mean systemic artery pressure 7 0.956 0.780 Mean pulmonary artery presure 7 0.841 0.102 Cardiac output 7 0.934 0.583 Mean airway pressure 7 0.988 0.989 Tidal volume 7 0.868 0.179 Arterial oxygen tension 7 0.829 0.079 Arterial carbon dioxide tension 7 0.971 0.903 Alveolar-arterial oxygen tension difference 7 0.975 0.932 Mean log (V/Q) 7 0.944 0.672 Mean SDlog (V/Q) 7 0.869 0.183
Qcvtotal 7 0.917 0.449
Qcvgravitational 7 0.927 0.523
Qcvisogravitational 7 0.929 0.544
Vcvtotal 7 0.873 0.195
Vcvgravitational 7 0.868 0.178
Vcvisogravitational 7 0.830 0.080
Q β1 7 0.963 0.852
V β1 7 0.923 0.493
V/Q β1 7 0.976 0.938
Q, perfusion; V, ventilation; CV, coefficient of variation; cNOS, constitutive nitric oxide synthase. β1 = slope of relationship between Q, V or V/Q and horizontal plane number.
Table E2. Effects of time on hemodynamics, gas exchange and distribution of lung perfusion.
Repeated measurements taken about 60 min apart in 5 animals
Baseline 1,
mean (SD)
Baseline 2, mean (SD)
Difference (Baseline 1 – Baseline 2),
mean (99 % CI) Arterial oxygen tension, kPa 13.1 (1.5) 13.3 (0.9) -0.2 (-3.7 to 3.3) Arterial carbon dioxide
tension, kPa
4.3 (0.6) 4.4 (0.7) -0.1 (-1.4 to 1.2)
Alveolar-arterial oxygen tension difference, kPa
3.1 (1.1) 2.9 (0.6) 0.2 (-2.3 to 2.7)
QCV total 0.43 (0.04) 0.41 (0.05) 0.02 (-0.03 to 0.08)
Cardiac output, l/min 3.9 (0.9) 3.0 (0.7) 0.9 (0.1 to 1.6) a Mean systemic arterial
pressure (mmHg)
139 (8.0) 137 (11.6) 2 (-27 to 31)
Mean pulmonary arterial pressure (mmHg)
21 (4.6) 23 (4.3) -2 (-6 to 2)
N=5 for all analyses. Q, perfusion; CV, coefficient of variation. a P=0.006 paired t-test.