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Enzyme Kinetic Analysis with Inhibitor and Immobilization Effects

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Monika Dwi Lestari

Academic year: 2024

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[I] 15 mM

[E] 5 mM

Tanpa Inhibitor Inhibitor A Immobilized

[S] (mM) Vo (mM/menit) Vo (mM/menit) Vo 1/[S]

5 5 2.87 2.2 0.2

7 6 3.6 2.8 0.142857

8 6.5 4 3 0.125

9 6.8 4.3 3.2 0.111111

10 7 4.7 3.4 0.1

15 8.5 6 4 0.066667

17 8.8 6.4 4.3 0.058824

20 9.1 6.8 4.6 0.05

25 9.8 7.4 4.9 0.04

y = 1.544x + 0.1416 R² = 0.9984

y = 1.3644x + 0.0785 R² = 0.9986

y = 0.6132x + 0.0783 R² = 0.9978

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5

0 0.05 0.1 0.15 0.2 0.25

Tanpa Inhibitor Inhibitor A Immobilized Linear (Immobilized) Linear (Inhibitor A) Linear (Tanpa Inhibitor)

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Tanpa Inhibitor Inhibitor A Immobilized

1/V0 1/V0 1/V0

0.2 0.348432056 0.454545455 0.166666667 0.277777778 0.357142857

0.153846154 0.25 0.333333333

0.147058824 0.23255814 0.3125 0.142857143 0.212765957 0.294117647

0.117647059 0.166666667 0.25

0.113636364 0.15625 0.23255814 0.10989011 0.147058824 0.217391304 0.102040816 0.135135135 0.204081633

Tanpa Inhibitor Inhibitor A Immobilized

Slope 0.6132 1.3644 1.544

Intercept 0.0783 0.0785 0.1416

Vm 12.77139208 12.7388535 7.062146893

Km 7.831417625 17.38089172 10.9039548

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