Evalua&on of Tumor Infiltra&ng Lymphocytes
(TILs)
in High-‐grade Serous Ovarian Carcinoma
Guidelines for TILs assessment from the “Interna5onal
Immuno-‐Oncology Biomarker Working Group”
Authors: Giancarlo Pruneri and Andrea Vingaini European Ins5tute of Oncology and University of Milan, Italy.
• TILs within the borders of the invasive tumors are evaluated
• The invasive edge is included in the evalua5on, but not
reported separately
• Immune infiltrates outside of the tumor borders are not included
Define area for TILs evalua&on
Evaluate TILs in this area
Exlude this area
• TILs within the borders of the invasive tumors are evaluated
• The invasive edge is included in the evalua5on
• Immune infiltrates outside of the tumor borders are not included
Define area for TILs evalua&on
Evaluate TILs in this area Exclude this area
Include TILs in this area
= stromal TILs
Define area for TILs evalua&on
Include TILs evalua5on in stroma pertaining to fibro-‐
vascular cores of papillary structures
Define area for TILs evalua&on
Include TILs evalua5on in stroma pertaining to fibro-‐vascular cores of papillary structures
Define area for TILs evalua&on
Include TILs evalua5on in stroma pertaining to fibro-‐vascular cores of papillary structures
Define area for TILs evalua&on
In case of ovarian or
peritoneal superficial tumor localiza5ons, evaluate TILs in connec5ve 5ssue just beneath the epithelial layer within 1mm.
Include this area
1mm
Areas of necrosis or fibrosis are not
included in the evalua5on
Do not include this area
Include TILs in this area
= stromal TILs
Define area for TILs evalua&on
Determine the localiza&on of inflammatory infiltrate
Stromal TILs (sTILs) Intra-‐tumoral TILs
(iTILs)
