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Supplemental Table 1. Discontinuous EEG Activity in Neonates

PMA*

EEG Features

Term Clinical Correlate Suppression

Periods Length

Suppression Amplitude (Voltage)

Burst Period Length

Burst Amplitude

(Voltage) Reactivity

> 30 weeks >10 seconds <5 μV 1-2 seconds

Theta and delta waves 75 to 250 μV

Not reactive to

external stimuli Burst Suppression

Associated with poor outcomes such as encephalitis and hypoxemic injury

30-35 weeks > 2 seconds 5-25 μV

(often <10 μV) - up to 300 μV Reactive to external stimuli

Discontinuous EEG activity

Normal discontinuous tracing of the very immature premature infant

> 36 weeks 3-15 seconds >25 μV

(25–50 μV) -

Slow waves 100 to 200 μV (50-150)

Reactive to external

stimuli Tracé alternant Quiet sleep of the infant. May persist up to 4 weeks after birth

*Postmenstrual Age (PMA). In older neurological literature the term “postconceptional age,” defined as gestational age (in weeks) plus the number of weeks since birth, is used instead of PMA.

Reactivity: A phenomena in which the EEG pattern clearly and reproducibly changes with sensory (visual, auditory, or noxious) stimulation. Clinical response includes active movements and respiratory pattern changes. EEG response may include changes in frequency, morphology, and/or amplitude, including attenuation of activity after stimulus and increased continuity.

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Supplemental Figure 1. Example of EEG Reactivity.

Example of EEG reactivity: Spectrogram of electroencephalogram of a 9-week-old premature infant (GA 29, PMA 38; ASA status III) under spinal anesthesia for inguinal herniorrhaphy. The spectrogram above shows discontinuous activity (~50-52 minutes) characterized by an alternating pattern of burst events lasting a few seconds followed by periods of suppression of variable duration and amplitude. At ~52 and ~56 minutes high-power activity across all the frequencies appears consistent with muscle activity from patient movement induced by clinically annotated “chin-lift.” After these events the EEG activity regains continuity.

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