1
Improved survival in a long-term rat model of sepsis is associated with reduced mitochondrial calcium uptake despite increased energetic demand
Supplemental Digital Content 3 – Characterization of cohort 1
Clinical severity score, blood pressure and biochemical parameters of disease.
Figure 1: Clinical severity and mean arterial pressure. A - Septic animals presented with clinical signs of illness including marked piloerection, hunched back, a bloated abdomen, conjunctival injection, markedly decreased movement and less interaction with their surroundings. Consequently, septic animals had a higher clinical severity, returning to control level at 72 h. B – Blood pressure was similar in septic animals and sham-controls. Legend: a: p<0.05 vs. sham. MAP: mean arterial pressure.
2 Table 1: Biochemical parameters of oxygenation, metabolism and inflammation. Blood oxygenation was unaffected by sepsis. At 24 h septic animals presented higher lactate levels. IL-6 and IL-10 were elevated in sepsis but due to a large variation and relatively small sample size this difference didn’t reach statistical significance. Legend: Hb – haemoglobin; n.s. - non significant; p-value is for T-test or Mann-Whitney; SaO2 –
3 oxygen saturation; * - p<0.05 vs. 6h. At 6 h n= 4 (sham) and 9 (sepsis). At 24 h n=4 (sham) and 8 (sepsis).
Sham Sepsis p-value
pH 6h 7.45±0.01 7.50±0.01 0.014
24h 7.45±0.04 7.51±0.03* ns
PaO2 (kPa) 6h 14.2±0.9 12.8±0.3 n.s.
24h 14.0±0.2 15.3±1.9 n.s.
PaCO2 (kPa) 6h 4.5±0.3 3.5±0.2 0.047
24h 4.3±0.1 3.6±0.3 n.s.
SaO2 (%) 6h 96.3±0.9 97.1±0.7 n.s.
24h 97.1±0.6 98.0±0.6 n.s.
Hb (g/dl) 6h 12.9±0.6 15.2±0.4 0.012
24h 12.0±0.8 13.0±0.6* n.s.
Lactate (mmol/l) 6h 1.53±0.24 1.19±0.14 n.s.
24h 0.73±0.18* 2.29±0.31* 0.002
Glucose (mmol/l) 6h 6.81±0.52 7.50±0.86 n.s.
24h 6.30±0.61 7.60±0.87 n.s.
IL-6 (pg/ml) 6h 1846±568 3226±386 n.s.
24h 2384±1456 11086±4699 n.s.
IL-10 (pg/ml) 6h 44±35 339±68 n.s.
24h 110±35 408±112 0.034