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The results showed that the RS content of arrowroot starch persisted after steam cooling treatment. Then, arrowroot starch substitution without or with modification also reduced the lightness and tensile strength of wet noodles. The tensile level of wet noodles decreased by 20% due to the replacement of wheat flour with arrowroot starch, without treatment or modification.

Furthermore, statistical analysis found that there was a significant difference between control and wet noodles with modified arrowroot starch replacement of 20%. It can be seen that the moisture content of wet noodles with modified arrowroot starch replacement was higher than that of control wet noodles, and arrowroot starch replaced wet noodles without treatment. This suggests that the substitution of modified arrowroot starch caused an increase in the moisture content of wet noodles.

The value of ash content in wet noodles with or without substitution of modified arrowroot starch experienced a decrease in the reduction of mineral content of arrowroot starch during the steam cooling cycle. In the statistical analysis, significant differences were found between the samples of control and arrowroot starch substitution without treatment and wet noodles with modified arrowroot starch substitution. Wet noodles with modified or unmodified arrowroot starch will contribute more carbohydrates than wet noodles at 100%.

Table 1.   Resistant Starch content and color of arrowroot  starch  with  and  without  steam-cooling  treatment
Table 1. Resistant Starch content and color of arrowroot starch with and without steam-cooling treatment

Assessing the Genotoxicity Effect of a Commercial Chlorpyrifos Formulation in Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae) Under Acute

The treatment was carried out in a plastic container containing 3 L of the test solution with ten Gosner 25 tadpoles [41] with three replications for each concentration. Most of the erythrocytes exposed to chlorpyrifos had a single micronucleus (Fig. 1A), while some cells had more than one micronucleus (Fig. B), and some had larger micronucleus sizes. 25], exposure to chlorpyrifos from the tadpoles Bufo bufo gargarizans showed the presence of nuclear pyknosis, binucleated erythrocytes and changes in erythrocyte cell morphology.

The results of the acute exposure genotoxicity test showed the potential for low doses of commercial chlorpyrifos formulations to cause DNA damage in erythrocyte cells of F. This variation is most likely caused by differences in the resistance of the test species. Long-term exposure of the commercial formulation of chlorpyrifos showed the potential to cause DNA damage with the formation of micronuclei in erythrocytes of F.

The results of the genotoxicity test showed a positive correlation between the concentration of chlorpyrifos and an increase in the frequency of micronuclei (MN). In summary, the commercial formulation of chlorpyrifos (Dursban 200EC) can cause DNA damage in erythrocyte cells of F. MN and morphological changes of the erythrocyte nucleus can trigger genetic mutations, metabolic damage, pathology and pathophysiological conditions, so it can affect or reduce physical conditions, species survival and loss of local diversity.

As this is the first study to examine the genotoxic effect of chlorpyrifos on F. Understanding the impact of chemicals on amphibians: a meta-analytic review: the impact of pollution on amphibians. Assessment of genotoxicity in Bufo raddei toads exposed to petrochemical pollutants in the Lanzhou region of China.

Micronucleus induction in erythrocytes of Hyla pulchella tadpoles (Amphibia: . Hylidae) exposed to insecticide endosulfan. Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at the larval stage of development.

Figure 1.   Erythrocytes of F. limnocharis tadpole exposed to the commercial organophospate insecticide Chlorpyrifos (1 µg.L -1 )
Figure 1. Erythrocytes of F. limnocharis tadpole exposed to the commercial organophospate insecticide Chlorpyrifos (1 µg.L -1 )

Phytochemical Analysis of Purple Sweet Potatoes (Ipomoea batatas) Roots Extract From Lawang and Kawi Mountain Cultivar, East Java, Indonesia

Phytochemical analysis of root extract of purple sweet potato (Ipomoea batatas) from Cultivar Lawang and Kawi mountains, East Java, Indonesia. The differences of Lawang and Mount Kawi total anthocyanin purple sweet potatoes were analyzed using an independent sample t-test by SPSS software. Meanwhile, the IC50 difference between ascorbic acid of cultivar Lawang and Kawi Mountain was analyzed using one-way ANOVA.

The PSP Lawang cultivar was not significantly different from the PSP Kawi Mountain cultivar at the IC50 value, i.e., 2.5 and 2.3 µg.mL-1 for Lawang and Kawi Mountain, respectively (Fig. 2). Next, the antioxidant activity of Lawang cultivar was relatively similar to that of Kawi Mountain cultivar. The higher antioxidant activity is also influenced by the presence of phenolics and flavonoids in purple sweet potatoes [21].

Although both cultivars have similar wavelength hump peaks of absorbance, the 521 nm absorbance was higher in the Lawang cultivar than the Kawi Mountain cultivar. The Lawang and Kawi Mountain region have distinct environmental characteristics such as temperature and soil pH. The soil pH in the Kawi mountain region is more acidic than Lawang, i.e. 4.71 and 5.25 respectively.

Purple sweet potatoes from Lawang and Kawi Mountain region of East Java Indonesia have similar qualitative phytochemical profiles and antioxidant activity. Effect of purple sweet potato anthocyanin against TNF-α, apoptosis and spatial memory in hippocampus of rat model of Diabetes Mellitus. Effect of extract purple Ipomoe batatas cultivar Kawi mountain chronic inflammation in Wistar rats with atherogenic diet.

Differential amino acid profiling of two morphologically purple sweet potatoes from cultivars of Mount Kawi, East Java, Indonesia. Optimization of natural pigment extraction from purple sweet potato by response surface methodology and its stability.

Figure 1.   Qualitative phytochemical profies from extracts  of  purple  sweet  potatoes  Lawang  and  Kawi  Mountain  cultivars
Figure 1. Qualitative phytochemical profies from extracts of purple sweet potatoes Lawang and Kawi Mountain cultivars

Analysis of Microplastics in Water and Biofilm Matrices in Metro River, East Java, Indonesia

This study aims to analyze the content of microplastics in the water and the biofilms growing in the Metro River. The high temperature value will accelerate the degradation process so that the abundance of microplastics in the waters is high [14]. The relationship between current velocity and abundance of microplastics in waters is unidirectional.

The identification of microplastics carried out in this study identified several types of microplastics, namely films, fibres, fragments and beads. Abundance of microplastics in water The abundance of microplastics in the water samples obtained in this study varied at each sampling station (Figure 3). The highest abundance of microplastics was at station 2, because the site was affected by waste from anthropogenic activities from densely populated settlements.

The amount of microplastics inside the biofilm materials obtained in this study was different at each sampling station, as shown in Figure 4. To compare the amount of microplastics in the biofilm matrix and water, the amounts of microplastics in the biofilm and the river water around the biofilm were compared (Fig .5). The results showed that the amount of microplastics in the biofilm was ten times higher than the amount of microplastics in water at each sampling station.

This study was conducted to identify the abundance of microplastics in the Metro River, Malang. The types of microplastics at all sampling stations consisted of fibres, fragments, films and beads. The amount of microplastics in river water varied from particles.mL-1 and inside the biofilm matrix varied from 7.4 - 9.5 particles.g-1.

The presence of microplastics in water, sediment and milkfish (Chanos chanos) at the downstream area of ​​Citarum River, Indonesia. Impacts of biofilm formation on the fate and potential effects of microplastics in the aquatic environment.

Figure 1. Sampling location
Figure 1. Sampling location

Synergism of Lecanicillium lecanii (Zimm) and Chromolaena odorata L. Leaf Extract to Control Aphis gossypii (Glover) in Chili Plants

Measurement of the diameter of fungal colonies on the 7th day after application (DAA) with a ruler. The results of observations of the number of conidia were collected and calculated by equation 3 [19]. Harvesting conidia on PDA media according to treatment, then taking a suspension of L.lecanii conidia and incubating on glass slides and placing them on a wet tissue placed on a tray and covered with plastic wrap for 24 hours.

Aphid inoculation is done using a soft brush on the underside of the chili pepper leaves. The effectiveness of an antifungal substance is influenced by the concentration of the given substance [24]. Saponin compounds in Ambon banana stem also have an antifungal activity that inhibits the growth of Candida albicans fungi [30].

In LK1 treatment, the addition of C. leaf extract with a concentration of 10% significantly inhibited L. lecanii germination with a decrease of 8.24% with a viability value of 69.00%. This was followed by LK2 treatment with the addition of C. leaf extract with a concentration of 25%, which has a viability value of 67.60% with a decreasing percentage of germination 10.11%. odorata L. leaf extract with a concentration of 40%) showed a viability value of 67.00% and a percentage decrease in germination of 10.90%. The higher the concentration of C. Conidia germination rate and high sporulation can be seen from good fungal physiology, where high conidia germination rate will also increase the virulence ability of the fungus [34].

Persistence is positively related to the level of fungal infection, where the higher the spore persistence, the faster the fungus produces spores, making the infection process in insects faster [35]. The results of the toxicity test showed the isolated application of the suspension of L. In the control treatment that was sprayed with aquadest, none of the aphid nymphs died.

The evaluation was evident from the change in the color of the body of the aphid, which was black and. The body surface of the host insect is more rapidly hydrolyzed by enzymes produced by the fungi, resulting in insect death [39]. Compatibility of the fungus Beauveria bassiana (Bas.) Vuill. Deuteromycetes) with neem seeds and leaves and emulsifying oil.

All types of published corrections can also be peer-reviewed at the editor's discretion.

Table 1 shows that the addition of  C. odorata  L.  leaf  extract  in  various  concentrations  also  significantly  affected  the  number  of  spores  (sporulation) of L
Table 1 shows that the addition of C. odorata L. leaf extract in various concentrations also significantly affected the number of spores (sporulation) of L

Title Typed in Bold, Capitalize each First Letter of Each Word, Except Conjunctive, Scientific name should not be Abbreviated

Calibri 14 Bold Center, should not exceed 12 words, except conjuctive)

Title in bold, capitalize every first letter of every word, except for the link, the scientific name must not be abbreviated. Halftone combination, combine image and text (image containing text) and graphics in color or grayscale format. Black and white image should be in grayscale mode, while color images should be in RGB mode.

If the figures come from the third party, they must transfer the copyright of the sources. References typed into numbering list (format number 1,2,3,…), arranged sequentially as they appear in the text (Vancouver system or author number style). In the manuscript, only the reference number is typed (not the author and year), for example: Obesity is an accumulation of fat in large quantities that would cause excessive body weight (overweight) [1].

The conclusions of the study's findings are written in a short, concise and solid manner, without additional new interpretations. This section can also be written about the novelty of the research, advantages and disadvantages of the research, and recommendations for future research. (Calibri 10 Justify). This section describes the acknowledgment to those who have helped in kind as well as financially.

Importance of CD80/CD86-CD28 interaction in the recognition of target cells by CD8+CD122+ regulatory T cells.

Table  should  be  submitted  within  the  manuscript  and  in  separated  file  of  Microsoft  Excel (xls.)
Table should be submitted within the manuscript and in separated file of Microsoft Excel (xls.)

Gambar

Table 1.   Resistant Starch content and color of arrowroot  starch  with  and  without  steam-cooling  treatment
Table  1  also  presents  the  lightness  level  of  arrowroot starch without treatment, 1 cycle to 3  cycles, which were found at 93.33, 82.54, 82.115,  and  80.93,  respectively
Table 3. Chemical properties of wet noodles
Table  4  shows  the  consumer  acceptance  of  wet noodles formulated with modified arrowroot  starch  substitution  using  formulations  of  5%,  10%,  and  20%
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