Supplemental Figure 3: Water maze test was performed at age of 52 wks for CVN and WT male mice.
Testing was performed in a large dark-coloredtank (120 cm in diameter) filled with clear water at a temperatureof 24.0 ± 1.0 °C. A submerged platform (14 x 14 cm; 1.5 cmbelow water surface) was placed in one of the quadrants. Animals were tested for 4 trials a day for 6 days with a hidden platform in a constant position (acquisition) after which the platform location was changed and the animals were tested again for 4 trials a day for two 2 days (reversal). Trial duration was 60 s. Animals were allowed to stay on the platform for 15 s after each trial. Latency to find the platform, swim distance and speed, and
thigmotactic behavior were recorded using HVS Image Video Tracking software (HVS Image, Mountain View CA). Statistical analysis was done using 2 way ANOVA and Prizm GraphPad Software (San Diego CA). Significance was set at p < 0.05.
CVN mice showed significantly increased latency and distance to find the hidden platform during the reversal learning when the platform location was changed to a new location. Moreover, their swim speed was significantly reduced and thigmotactic behavior increased during reversal training.
