Supplemental table 2: Kingella kingae PCR assay performance characteristics for related bacteria, spiked samples and for clinical samples with results compared to reference results
Sample No.
Specimen
type Description
*Reference result
K. kingae PCR result 1
Reference
strain Kingella kingae (ATCC 23330) Positive Positive 2 Reference
strain Streptococcus pneumoniae
(ATCC 49619) Negative Negative
3 Reference
strain Haemophilus influenzae (ATCC
10211) Negative Negative
4
Reference strain
Pseudomonas aeruginosa
(ATCC 27853) Negative Negative
5
Reference strain
Staphylococcus aureus (ATCC
43300) Negative Negative
6
Reference strain
Staphylococcus epidermidis
(ATCC 12228) Negative Negative
7
Reference strain
Streptococcus agalactiae
(ATCC 13813) Negative Negative
8 Reference
strain Streptococcus mitis (ATCC
6249) Negative Negative
9 Reference
strain Streptococcus pyogenes (ATCC
19615) Negative Negative
10
Reference strain
Neisseria gonorrhoeae (ATCC
19424) Negative Negative
11
Reference strain
Neisseria meningitidis (ATCC
13077) Negative Negative
12
Reference
strain Vibrio cholerae (ATCC 14035) Negative Negative 13
Reference strain
Salmonella cholerasuis (ATCC
14028) Negative Negative
14
Clinical
specimen Joint fluid Negative Negative
15 Clinical
specimen Joint fluid Negative Negative
16 Clinical
specimen Joint fluid Negative Negative
17
Clinical
specimen Joint fluid Negative Negative
18
Clinical
specimen Joint fluid Negative Negative
19
Clinical
specimen Heart Valve Positive Positive
20
Clinical
specimen Blood Positive Positive
21 Clinical
specimen Joint Fluid Positive Positive
22 Spiked
specimen Synovial fluid Positive Positive
23
Spiked
specimen Tibial bone Positive Positive
24
Spiked
specimen Synovial fluid Positive Positive
25
Spiked
specimen Bone Positive Positive
26
Spiked
specimen Pericardial fluid Positive Positive
27
Spiked
specimen Synovial fluid Positive Negative
28 Spiked
specimen Ankle tissue Positive Positive
29 Spiked
specimen Bone Positive Positive
30
Spiked
specimen Knee tissue Positive Positive
31
Spiked
specimen Blood Positive Positive
32
Spiked
specimen Blood Positive Positive
33
Spiked
specimen Throat swab Positive Positive
34
Spiked
specimen ENAT swab Positive Positive
Spiking, DNA extraction and PCR assays were performed as described in the Methods section of the manuscript.
*Reference results based on information of known ATCC strains or culture and 16s rRNA gene sequencing results.