-5 min 30 min 45 min 75 min 105 min Mean arterial pressure MetNonprod+Air Median

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-5 min 30 min 45 min 75 min 105 min Mean arterial pressure

MetNonprod+Air Median

25p; 75p

82.6

72.5; 93.4

87.2

66.5; 95.8

76.2

57.9; 87.3

71.3

60.6; 79.8

84.3

71.4; 90.7

MetNonprod+IR Median

25p; 75p

68.2

60.0; 78.9

70.5

54.2; 83.0

54.2

50.6; 69.3

67.9

59.0; 79.5

64.1

52.5; 79.8

MetNonprod+IR+Met Median

25p; 75p

62.4

51.1; 76.3

83.7

64.6; 90.5

53.5

47.7; 61.1

59.8

54.4; 72.1

55.1

50.6; 79.1

MetNonprod+Met+IR Median

25p; 75p

64.9

49.8; 82.6

63.4

46.0; 86.3

48.8

38.4; 74.7

55.6

44.1; 85.8

67.8

48.7; 84.3

MetProd+IR Median

25p; 75p

69.0

61.5;82.1

78.7

66.3; 93.1

59.6

44.1; 68.3

62.4

55.9; 82.8

57.7

46.6; 82.1

Superior mesenteric artery flow MetNonprod+Air Median

25p; 75p

6.67

5.17; 8.15

8.4

4.85; 14.1

9.2

6.7; 14.2

6.7

4.7; 8.7

8.8

4.4; 10.5

25p; 75p

7.1

6.86; 8.4

0.0^a,b

0.0; 0.0

8.2^a

6.87; 11.7

7.1

4.5; 8.96

6.9

2.97; 9.1

25p; 75p

5.95

5.33; 8.72

0.0^a,b

0.0; 0.0

6.6

5.6; 7.3

4.2^a,b

2.5; 4.8

3.8^a,b

2.6; 4.0

25p; 75p

6.96

6.55; 7.75

0.0^a,b

0.0; 0.0

9.3

4.1; 13.1

5.45

3.6; 7.7

5.3^a

4.0; 6.6

25p; 75p

5.85

5.5; 7.0

0.0^a,b

0.0; 0.0

6.98

5.95; 12.03

5.0

3.7; 5.4

3.1^a

2.7; 5.2

Exhaled methane levels MetNonprod+Air Median

25p; 75p

0.46^d

0.32; 0.69

0.41

0.29; 0.5

0.45

0.19; 0.74

0.40

0.29; 0.54

0.4

0.27; 0.47

MetNonprod+IR Median

25p; 75p

0.34^d

0.21; 0.8

0.31

0.14; 0.49

0.72

034; 0.86

0.38

0.2; 0.72

0.38

0.2; 0.72

25p; 75p

0.27^d

0.09; 0.74

0.2

0.07; 0.31

0.93^a

0.7; 1.25

0.75

0.38; 0.85

0.6

0.4; 0.87

25p; 75p

1.93^c

1.4; 2.5

0.44^a

0; 0.6

2.01^b

1.4; 2.4

1.25

0.67; 1.78

1.1^b,c

0.74; 1.3

25p; 75p

1.08^c,e

0.98; 1.59

0.54^e

0.49; 0.65

1,08

0.56; 1.66

0.78

0.34; 0.97

0.66^a

0.30; 0.76

Table S2. Macrohemodynamic data and exhaled methane levels in rats. Mean arterial pressure [mmHg], superior mesenteric artery flow [mL min^-1] and exhaled methane concentrations [ppm] are shown. ^aP<0.05 within group vs. baseline values (Friedman and Dunn tests). ^bP<0.05 groups vs. MetNonprod+Air group values; ^cP<0.05 groups vs. MetNonprod+IR group values;

dP<0.05 groups vs. MetNonprod+Met+IR group values; ^eP<0.05 groups vs.

MetNonprod+IR+Met group values (Kruskal-Wallis and Dunn tests).

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-5 min 60 min Mean red blood cell velocity

MetNonprod+Air Median

25p; 75p

562.8

551.3; 583.5

550.6

461.3;624

25p; 75p

617.5

545.8; 698.5

558.8

443.7; 708.3

25p; 75p

656.6

548.8; 772.1

733.8

686.6;757.8

25p; 75p

567.9

529.6; 620.2

475.3

318.1;642.2

25p; 75p

691.8

554.8; 755.4

650.9

526.1; 727.5

Exhaled methane levels MetNonprod+Air Median

25p; 75p

0.44

0.33; 0.6

0.38

0.18; 0.53

25p; 75p

0.42

0.24; 0.78

0.21^a

0.01; 0.43

25p; 75p

0.25

0.09; 0.36

0.99^a

0.59; 1.45

25p; 75p

1.45^e

0.41; 2.26

1.19^c

0.84; 1.7

25p; 75p

0.97^e

0.72; 1.21

0.74

0.34; 0.95

Table S3. Small intestinal microcirculation in rats. Mean red blood cell velocity (RBCV) in the ileal serosa [µm s^-1] and exhaled methane concentrations [ppm]. ^aP<0.05 within group vs.

baseline values (Friedman and Dunn tests). ^bP<0.05 groups vs. MetNonprod+Air group values;

cP<0.05 groups vs. MetNonprod+IR group values;^eP<0.05 groups vs. MetNonprod+IR+Met group values (Kruskal-Wallis and Dunn tests).

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MPO XOR NOX

MetNonprod+Air Median

25p; 75p

870

588; 910

71.0

61.3; 77.9

8.16

7.52; 8.66

25p; 75p

2217^b

1711; 2389

110.2^b

97.9; 118.2

14.81^b

13.71; 15.95

25p; 75p

2196^b

1758; 2581

107.5^b

96.5; 118.1

13.75^b

12.96; 14.77

25p; 75p

2699^b

1991; 3643

110.0^b

97.1; 123.0

13.25^b

12.63; 14.91

25p; 75p

1908^b

1549; 2003

114.0^b

86.5; 148.5

16.00^b

11.00;20.00

Table S4. Tissue inflammatory markers in the rat ileum. Myeloperoxidase (MPO) activity [mU (mg protein)^-1], xanthine oxidoreductase (XOR) activity [pmol min^-1 (mg protein)^-1], and nitrite and nitrate (NOx) levels [µM (mg protein)^-1] are presented. ^bP<0.05 groups vs.

MetNonprod+Air group values (Kruskal-Wallis and Dunn tests).

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Baseline Occlusion 1 Reperfusion 1 Occlusion 2 Reperfusion 2 Occlusion 3 Reperfusion 3 Occlusion 4 Reperfusion 4 Mean arterial pressure

Median 25p; 75p

124.0 118.8; 147.3

129.0 123.5; 141.0

135.0 113.5; 153.5

136.5 132.5; 149.0

127.5 109.8; 160.5

124.0 119.5; 155.0

119.0 104.3; 136.8

109.5 102.8; 126.3

111.0 97.3; 142.8 Heart rate

Median 25p; 75p

86 77; 113

84 70; 100

80 73; 92

82 74; 87

83 73; 99

84 74; 97

89 75; 103

89 71; 94

91 66; 101 Cardiac index

Median 25p; 75p

3.16 2.16; 3.60

2.43 1.81; 3.19

2.50 2.19; 2.96

2.64 2.21; 3.20

2.66 2.21; 3.20

2.58 2.24; 3.16

2.70 2.19; 3.49

2.78 2.32; 3.18

2.71 2.42; 3.23

Table S5. Graded superior mesenteric artery occlusion in pigs. Mean arterial pressure [mmHg], heart rate [beat min^-1] and cardiac index [L min^-1 m^-2] are shown.