CD36-ABCA1+ Macrophages and CAD Risk Markers in T2DM (Irawati N, et al.) Indones Biomed J. 2023; 15(6): 400-10 DOI: 10.18585/inabj.v15i6.2625
Supplementary 1.
Gating strategy for flowcytometry analysis for Non-DM/healthy donor subject.
Non-DM (-) Non-DM (+) Ox-LDL Non-DM (+) LPS
S1
The Indonesian Biomedical Journal, Vol.15, No.6, December 2023, p.351-436 Print ISSN: 2085-3297, Online ISSN: 2355-9179
DM (-) DM (+) Ox-LDL DM (+) LPS
Supplementary 2.
Gating strategy for flowcytometry analysis for DM subject.
S2
CD36-ABCA1+ Macrophages and CAD Risk Markers in T2DM (Irawati N, et al.) Indones Biomed J. 2023; 15(6): 400-10 DOI: 10.18585/inabj.v15i6.2625
*
Non-DM DM 8
0.8
0.6
0.4
0.2
p=0.06
M=0.34 Non-DM
DM M=48
M=119 600
500 400 300 200 100 0
30 40 50 60 70 80 90
Triglyceride (mg/dL)
HDL (mg/dL)
HDL/LDL Ratio
A B
Supplementary 3.
Relationship between T2DM status, triglyceride, HDL/LDL ratio. A: Distribution of HDL-C and triglycerides in T2DM and non- T2DM subjects. The area was divided into 4 quadrants with a horizontal line that represent the median of triglyceride concentration (119.0 mg/dL) and a vertical line that represent the median of HDL (48 mg/dL). The red dot indicated T2DM patient and the green one indicated healthy donor. B: HDL/LDL ratio in healthy donors and T2DM subjects. HDL/LDL ratio was presented as boxplots and the significancy was assessed by Mann-Whitney test.
Supplementary 4.
IL-1β and IL-10 concentrations of macrophages cultures from healthy donors and T2DM subjects.
Cytokines Stimuli Healthy Subjects
(n=11)
T2DM Subjects
(n=13) p -value
IL-1β Ox-LDL, mean±SD 9.94±4.97 9.1±6.86 0.741
LPS, mean±SD 89.43±68.20 110.37±60.88 0.435
Unstimulated, median (min-max) 7.26 (3.10-18.47) 7.48 (0.78-20.38) 0.649 IL-10 Ox-LDL, median (min-max) 41.13 (18.05-91.01) 45.60 (5.54- 117.38) 0.608 LPS, median (min-max) 664.71 (146.93-2300.4) 851.61 (73-1744.42) 0.649 Unstimulated, median (min-max) 53.04 (13.25-168.96) 34.85 (2.89-222.68) 0.459
IL-1β/IL-10 Ox-LDL, mean±SD 0.23±0.09 0.35 ± 0.31 0.195
LPS, median (min-max) 0.09 (0.07-0.27) 0.13 (0.06-0.59) 0.106
Unstimulated, median (min-max) 0.12 (0.05-1.25) 0.18 (0.01-0.97) 0.277
S3
