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Supplemental Digital Content 6

GMT for HI antibodies pre-vaccination and 7 days after first vaccination in the revaccination study in children 30−48 months of age (per-protocol cohort)

Dotted line represents the assay cut-off (10 1/dil). GMT values are shown above the bars.

CI: confidence interval; GMT: geometric mean titer; HI: hemagglutination inhibition;

Pre: pre-vaccination; Post: 7 days following vaccination

1 10 100 1000

Pre A/H1N1

Pre A/H3N2

Pre

B/Yamagata Pre

B/Victoria

Post Post Post Post

Geometric mean titer(95% CI)

Primed (same vaccine strain as 2011-2012 season) Primed (different vaccine strain to 2011-2012 season) Unprimed

65.1

553.1

13.8

161.6

35.9

237.8

14.4

222.9

20.9

81.4

18.6

65.6

11.5

68.2

7.1

36.5

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